FvH4_5g22740

Blue copper protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0005440
Physical Location & Seq
Reverse (-)
14057524 .. 14059312
1789 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g22740.t1

Sequence Viewer

Length: 684 bp
ATGGAGCTGCTCATTACCTTATCATGTTTTCTCATTCTTGTTTCAGTTTCTGGTAATTCCGTTCTTGCCTACAAGAACCTCACGGTGGGCGACTCCTTGGGTTGGTACGATAACCAAGTAAAGCCTGATGTTGATTACCAGAAATGGGCTGCAGCCAACAACTTCAGCTTGGGAGATTTTCTCATTTTTAACACTGATACAAACCACTCGGTCGTTCAAACCTATAACGTGACAATTTACAAGTTGTGCGATTATGACAATGCCGAGGAGAAGGATGCAATCCAATGGTCGCAATCTGACCCGTCAAACACCGCCACACATGATGTGTCAGTGGCAGTTCCTTTGTTGAAAGAGGGCGTCACGTATTTCTTTTCCGGTGATTATGATGGCGATCAGTGCAAGAACGGGCAGCACTTCATGATAACCGTTACCCATGGGCAAGGCTTGCCTAAGAGTGGAGACGATTCCACGGCAGGACCAGCAAGCAGTCCACAATCAGGCAATGGTGACGATGAGTCGATCCCGGAGACAATTGTCCCTCATTCCAACTTCAATGATCCCAAAGAAGAGGATGATAGTGATAATGATAAAGAGACATCTGGATCCGTTTCATTTTCAATTCCTCATGTACAAGGCAATCTGATTTTCTTTTTGCTAGGTCTTGTGTACTTTTTGTGCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

228

Amino Acids

24.82

Weight (kDa)

4.17

Isoelectric Point (pI)

23.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 43 - 137 2e-14 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 312
AclWI GGATC 4 cut(s) 514, 551, 597, 610
AcuI CTGAAG 1 cut(s) 148
AcyI GRCGYC 1 cut(s) 357
AfaI GTAC 3 cut(s) 107, 630, 668
AfiI CCNNNNNNNGG 5 cut(s) 85, 102, 145, 455, 497
AgsI TTSAA 4 cut(s) 218, 349, 553, 618
AhdI GACNNNNNGTC 1 cut(s) 514
AluBI AGCT 2 cut(s) 7, 168
AluI AGCT 2 cut(s) 7, 168
Alw21I GWGCWC 1 cut(s) 680
Alw26I GTCTC 3 cut(s) 453, 521, 587
AlwI GGATC 4 cut(s) 514, 551, 597, 610
AlwNI CAGNNNCTG 1 cut(s) 50
ApeKI GCWGC 4 cut(s) 7, 149, 152, 409
AspS9I GGNCC 1 cut(s) 476
AsuC2I CCSGG 1 cut(s) 524
AsuHPI GGTGA 2 cut(s) 389, 518
AvaII GGWCC 1 cut(s) 476
BamHI GGATCC 1 cut(s) 602
Bbv12I GWGCWC 1 cut(s) 680
BbvI GCAGC 3 cut(s) 136, 164, 421
BccI CCATC 1 cut(s) 380
BceAI ACGGC 1 cut(s) 486
BcnI CCSGG 1 cut(s) 524
BcoDI GTCTC 3 cut(s) 453, 521, 587
BfaI CTAG 1 cut(s) 656
BfmI CTRYAG 1 cut(s) 150
BisI GCNGC 4 cut(s) 8, 150, 153, 410
BlsI GCNGC 4 cut(s) 9, 151, 154, 411
Bme1390I CCNGG 1 cut(s) 524
Bme18I GGWCC 1 cut(s) 476
BmeRI GACNNNNNGTC 1 cut(s) 514
BmgT120I GGNCC 1 cut(s) 476
BmiI GGNNCC 1 cut(s) 604
BmrFI CCNGG 1 cut(s) 524
BmsI GCATC 1 cut(s) 265
BoxI GACNNNNGTC 1 cut(s) 533
BplI GAGNNNNNCTC 2 cut(s) 165, 197
BpuMI CCSGG 1 cut(s) 524
BsaAI YACGTR 1 cut(s) 363
BsaBI GATNNNNATC 1 cut(s) 390
BsaHI GRCGYC 1 cut(s) 357
BsaJI CCNNGG 4 cut(s) 96, 264, 433, 468
BsaWI WCCGGW 1 cut(s) 374
Bsc4I CCNNNNNNNGG 5 cut(s) 85, 102, 145, 455, 497
Bse3DI GCAATG 1 cut(s) 508
Bse8I GATNNNNATC 1 cut(s) 390
BseDI CCNNGG 4 cut(s) 96, 264, 433, 468
BseGI GGATG 2 cut(s) 280, 577
BseJI GATNNNNATC 1 cut(s) 390
BseLI CCNNNNNNNGG 5 cut(s) 85, 102, 145, 455, 497
BseMI GCAATG 1 cut(s) 508
BseRI GAGGAG 1 cut(s) 281
BseXI GCAGC 3 cut(s) 136, 164, 421
Bsh1285I CGRYCG 1 cut(s) 213
BsiEI CGRYCG 1 cut(s) 213
BsiHKAI GWGCWC 1 cut(s) 680
BsiSI CCGG 2 cut(s) 375, 524
BslFI GGGAC 1 cut(s) 521
BslI CCNNNNNNNGG 5 cut(s) 85, 102, 145, 455, 497
BsmAI GTCTC 3 cut(s) 453, 521, 587
BsmBI CGTCTC 1 cut(s) 453
BsmFI GGGAC 1 cut(s) 521
Bsp1286I GDGCHC 1 cut(s) 680
Bsp1407I TGTACA 1 cut(s) 628
Bsp143I GATC 4 cut(s) 391, 519, 556, 602
Bsp19I CCATGG 1 cut(s) 433
BspACI CCGC 1 cut(s) 312
BspHI TCATGA 2 cut(s) 417, 680
BspLI GGNNCC 1 cut(s) 604
BspMAI CTGCAG 1 cut(s) 154
BspPI GGATC 4 cut(s) 514, 551, 597, 610
BsrDI GCAATG 1 cut(s) 508
BsrGI TGTACA 1 cut(s) 628
BssECI CCNNGG 4 cut(s) 96, 264, 433, 468
BssMI GATC 4 cut(s) 391, 519, 556, 602
BssNI GRCGYC 1 cut(s) 357
BssT1I CCWWGG 2 cut(s) 96, 433
Bst4CI ACNGT 2 cut(s) 85, 427
Bst6I CTCTTC 1 cut(s) 561
BstACI GRCGYC 1 cut(s) 357
BstAPI GCANNNNNTGC 1 cut(s) 445
BstAUI TGTACA 1 cut(s) 628
BstBAI YACGTR 1 cut(s) 363
BstC8I GCNNGC 2 cut(s) 446, 484
BstDEI CTNAG 1 cut(s) 450
BstDSI CCRYGG 2 cut(s) 433, 468
BstF5I GGATG 2 cut(s) 280, 577
BstKTI GATC 4 cut(s) 394, 522, 559, 605
BstMAI GTCTC 3 cut(s) 453, 521, 587
BstMBI GATC 4 cut(s) 391, 519, 556, 602
BstMCI CGRYCG 1 cut(s) 213
BstMWI GCNNNNNNNGC 3 cut(s) 396, 445, 479
BstPAI GACNNNNGTC 1 cut(s) 533
BstSCI CCNGG 1 cut(s) 522
BstSFI CTRYAG 1 cut(s) 150
BstV1I GCAGC 3 cut(s) 136, 164, 421
BstX2I RGATCY 1 cut(s) 602
BstYI RGATCY 1 cut(s) 602
BtgI CCRYGG 2 cut(s) 433, 468
BtsCI GGATG 2 cut(s) 280, 577
BtsIMutI CAGTG 3 cut(s) 192, 336, 401
Cac8I GCNNGC 2 cut(s) 446, 484
CaiI CAGNNNCTG 1 cut(s) 50
CciI TCATGA 2 cut(s) 417, 680
Cfr13I GGNCC 1 cut(s) 476
CseI GACGC 1 cut(s) 346
Csp6I GTAC 3 cut(s) 106, 629, 667
CviAII CATG 6 cut(s) 24, 320, 418, 434, 626, 681
CviJI RGCY 6 cut(s) 7, 124, 149, 155, 168, 444
CviKI_1 RGCY 6 cut(s) 7, 124, 149, 155, 168, 444
CviQI GTAC 3 cut(s) 106, 629, 667
DdeI CTNAG 1 cut(s) 450
DpnI GATC 4 cut(s) 393, 521, 558, 604
DpnII GATC 4 cut(s) 391, 519, 556, 602
DriI GACNNNNNGTC 1 cut(s) 514
Eam1104I CTCTTC 1 cut(s) 561
Eam1105I GACNNNNNGTC 1 cut(s) 514
EarI CTCTTC 1 cut(s) 561
Eco130I CCWWGG 2 cut(s) 96, 433
Eco47I GGWCC 1 cut(s) 476
Eco57I CTGAAG 1 cut(s) 148
EcoT14I CCWWGG 2 cut(s) 96, 433
ErhI CCWWGG 2 cut(s) 96, 433
Esp3I CGTCTC 1 cut(s) 453
FaeI CATG 6 cut(s) 27, 323, 421, 437, 629, 684
FaiI YATR 9 cut(s) 25, 225, 255, 321, 384, 419, 435, 627, 682
FaqI GGGAC 1 cut(s) 521
FatI CATG 6 cut(s) 23, 319, 417, 433, 625, 680
Fnu4HI GCNGC 4 cut(s) 8, 150, 153, 410
FokI GGATG 2 cut(s) 287, 584
Fsp4HI GCNGC 4 cut(s) 8, 150, 153, 410
FspBI CTAG 1 cut(s) 656
GluI GCNGC 4 cut(s) 8, 150, 153, 410
HapII CCGG 2 cut(s) 375, 524
HgaI GACGC 1 cut(s) 346
Hin1I GRCGYC 1 cut(s) 357
Hin1II CATG 6 cut(s) 27, 323, 421, 437, 629, 684
HinfI GANTC 3 cut(s) 92, 464, 515
HpaII CCGG 2 cut(s) 375, 524
HphI GGTGA 2 cut(s) 389, 518
Hpy166II GTNNAC 2 cut(s) 491, 667
Hpy188I TCNGA 2 cut(s) 298, 642
Hpy188III TCNNGA 3 cut(s) 418, 600, 681
Hpy8I GTNNAC 2 cut(s) 491, 667
HpyAV CCTTC 1 cut(s) 265
HpyCH4III ACNGT 2 cut(s) 85, 427
HpyCH4IV ACGT 2 cut(s) 228, 362
HpyCH4V TGCA 3 cut(s) 152, 278, 399
HpyF10VI GCNNNNNNNGC 3 cut(s) 396, 445, 479
HpyF3I CTNAG 1 cut(s) 450
HpySE526I ACGT 2 cut(s) 228, 362
Hsp92I GRCGYC 1 cut(s) 357
Hsp92II CATG 6 cut(s) 27, 323, 421, 437, 629, 684
Kzo9I GATC 4 cut(s) 391, 519, 556, 602
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 9 cut(s) 36, 138, 152, 388, 459, 483, 492, 537, 585
Lsp1109I GCAGC 3 cut(s) 136, 164, 421
LweI GCATC 1 cut(s) 265
MaeI CTAG 1 cut(s) 656
MaeII ACGT 2 cut(s) 228, 362
MaeIII GTNAC 4 cut(s) 229, 358, 427, 506
MalI GATC 4 cut(s) 393, 521, 558, 604
MboI GATC 4 cut(s) 391, 519, 556, 602
MboII GAAGA 1 cut(s) 578
MfeI CAATTG 1 cut(s) 531
MflI RGATCY 1 cut(s) 602
MhlI GDGCHC 1 cut(s) 680
MluCI AATT 4 cut(s) 55, 234, 531, 618
MlyI GAGTC 2 cut(s) 86, 524
MmeI TCCRAC 1 cut(s) 570
MnlI CCTC 6 cut(s) 89, 259, 346, 549, 562, 633
MseI TTAA 1 cut(s) 189
MspI CCGG 2 cut(s) 375, 524
MspR9I CCNGG 1 cut(s) 524
MunI CAATTG 1 cut(s) 531
MwoI GCNNNNNNNGC 3 cut(s) 396, 445, 479
NciI CCSGG 1 cut(s) 524
NcoI CCATGG 1 cut(s) 433
NdeII GATC 4 cut(s) 391, 519, 556, 602
NlaIII CATG 6 cut(s) 27, 323, 421, 437, 629, 684
NlaIV GGNNCC 1 cut(s) 604
NmeAIII GCCGAG 1 cut(s) 289
NmuCI GTSAC 3 cut(s) 229, 358, 506
PagI TCATGA 2 cut(s) 417, 680
PfeI GAWTC 1 cut(s) 464
PfoI TCCNGGA 1 cut(s) 522
PkrI GCNGC 4 cut(s) 9, 151, 154, 411
PleI GAGTC 2 cut(s) 86, 523
PpsI GAGTC 2 cut(s) 86, 523
Ppu21I YACGTR 1 cut(s) 363
PshAI GACNNNNGTC 1 cut(s) 533
PspN4I GGNNCC 1 cut(s) 604
PspPI GGNCC 1 cut(s) 476
PstI CTGCAG 1 cut(s) 154
PstNI CAGNNNCTG 1 cut(s) 50
PsuI RGATCY 1 cut(s) 602
RsaI GTAC 3 cut(s) 107, 630, 668
RsaNI GTAC 3 cut(s) 106, 629, 667
SaqAI TTAA 1 cut(s) 189
SatI GCNGC 4 cut(s) 8, 150, 153, 410
Sau3AI GATC 4 cut(s) 391, 519, 556, 602
Sau96I GGNCC 1 cut(s) 476
SchI GAGTC 2 cut(s) 86, 524
ScrFI CCNGG 1 cut(s) 524
SduI GDGCHC 1 cut(s) 680
SetI ASST 8 cut(s) 9, 20, 81, 170, 224, 231, 365, 661
SfaNI GCATC 1 cut(s) 265
SfcI CTRYAG 1 cut(s) 150
SinI GGWCC 1 cut(s) 476
Sse9I AATT 4 cut(s) 55, 234, 531, 618
SsiI CCGC 1 cut(s) 312
SspMI CTAG 1 cut(s) 656
StyD4I CCNGG 1 cut(s) 522
StyI CCWWGG 2 cut(s) 96, 433
TaaI ACNGT 2 cut(s) 85, 427
TaiI ACGT 2 cut(s) 231, 365
TaqI TCGA 1 cut(s) 518
TaqII GACCGA 1 cut(s) 199
TasI AATT 4 cut(s) 55, 234, 531, 618
TatI WGTACW 2 cut(s) 628, 666
TfiI GAWTC 1 cut(s) 464
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
TscAI CASTG 3 cut(s) 199, 336, 401
TseFI GTSAC 3 cut(s) 229, 358, 506
TseI GCWGC 4 cut(s) 7, 149, 152, 409
Tsp45I GTSAC 3 cut(s) 229, 358, 506
TspDTI ATGAA 2 cut(s) 406, 600
TspGWI ACGGA 2 cut(s) 49, 595
TspRI CASTG 3 cut(s) 199, 336, 401
VpaK11BI GGWCC 1 cut(s) 476
XspI CTAG 1 cut(s) 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.