Rorug05G0218200

Blue copper protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0005440
Physical Location & Seq
Forward (+)
22444424 .. 22444711
288 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0218200.1

Sequence Viewer

Length: 288 bp
ATGGCGGCTCTAGTTCTGTCCTCGGAGATCAAGGACACCCCGGACGGCGTCACCATCAAGTTGAGGGCCAACGTGATCGAAGTCGAAAGTCTGCGTCGGAAGTTCAACAGAAACTTCGAGCAAACCAATTTCGACTTCGAACTCGTCACTGAGGAGTCCAACGGCAAGAAGAATCTCAAGATGGAGGCTTGGTTCGGCACAGGCGAGACTAGAGCCGCCATCTGCGCCTCCTCGAGCCTCCGTGAGCGGCGTCCGCACTTTTTCATCTTTGCGGACATCCACCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

95

Amino Acids

10.89

Weight (kDa)

6.73

Isoelectric Point (pI)

44.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 247
AciI CCGC 5 cut(s) 5, 216, 247, 254, 272
AcyI GRCGYC 2 cut(s) 48, 250
AgsI TTSAA 1 cut(s) 106
AjuI GAANNNNNNNTTGG 2 cut(s) 119, 151
AloI GAACNNNNNNTCC 2 cut(s) 176, 208
Alw26I GTCTC 1 cut(s) 200
Ama87I CYCGRG 1 cut(s) 232
AoxI GGCC 1 cut(s) 66
AspLEI GCGC 1 cut(s) 227
AspS9I GGNCC 1 cut(s) 66
AsuC2I CCSGG 1 cut(s) 41
AsuHPI GGTGA 1 cut(s) 43
AsuII TTCGAA 1 cut(s) 138
AvaI CYCGRG 1 cut(s) 232
BccI CCATC 3 cut(s) 62, 175, 227
BceAI ACGGC 2 cut(s) 61, 178
BcnI CCSGG 1 cut(s) 41
BcoDI GTCTC 1 cut(s) 200
BfaI CTAG 2 cut(s) 11, 210
BisI GCNGC 3 cut(s) 6, 216, 248
BlsI GCNGC 3 cut(s) 7, 217, 249
Bme1390I CCNGG 1 cut(s) 41
BmeT110I CYCGRG 1 cut(s) 232
BmgT120I GGNCC 1 cut(s) 66
BmrFI CCNGG 1 cut(s) 41
Bpu14I TTCGAA 1 cut(s) 138
BpuEI CTTGAG 1 cut(s) 161
BpuMI CCSGG 1 cut(s) 41
BsaHI GRCGYC 2 cut(s) 48, 250
BsaJI CCNNGG 2 cut(s) 21, 39
BsaXI ACNNNNNCTCC 2 cut(s) 176, 206
BseDI CCNNGG 2 cut(s) 21, 39
BseGI GGATG 1 cut(s) 276
BseMII CTCAG 1 cut(s) 141
BseRI GAGGAG 2 cut(s) 167, 220
BshFI GGCC 1 cut(s) 68
BsiHKCI CYCGRG 1 cut(s) 232
BsiSI CCGG 1 cut(s) 41
BsmAI GTCTC 1 cut(s) 200
BsnI GGCC 1 cut(s) 68
BsoBI CYCGRG 1 cut(s) 232
Bsp119I TTCGAA 1 cut(s) 138
Bsp143I GATC 2 cut(s) 27, 75
BspACI CCGC 5 cut(s) 5, 216, 247, 254, 272
BspANI GGCC 1 cut(s) 68
BspCNI CTCAG 1 cut(s) 142
BspT104I TTCGAA 1 cut(s) 138
BsrBI CCGCTC 1 cut(s) 247
BssECI CCNNGG 2 cut(s) 21, 39
BssMI GATC 2 cut(s) 27, 75
BssNI GRCGYC 2 cut(s) 48, 250
BstACI GRCGYC 2 cut(s) 48, 250
BstBI TTCGAA 1 cut(s) 138
BstDEI CTNAG 1 cut(s) 150
BstF5I GGATG 1 cut(s) 276
BstHHI GCGC 1 cut(s) 227
BstKTI GATC 2 cut(s) 30, 78
BstMAI GTCTC 1 cut(s) 200
BstMBI GATC 2 cut(s) 27, 75
BstMWI GCNNNNNNNGC 2 cut(s) 224, 253
BstSCI CCNGG 1 cut(s) 39
BsuRI GGCC 1 cut(s) 68
BtsCI GGATG 1 cut(s) 276
BtsIMutI CAGTG 1 cut(s) 147
CfoI GCGC 1 cut(s) 227
Cfr13I GGNCC 1 cut(s) 66
CseI GACGC 3 cut(s) 37, 83, 239
CviJI RGCY 5 cut(s) 8, 68, 188, 215, 237
CviKI_1 RGCY 5 cut(s) 8, 68, 188, 215, 237
DdeI CTNAG 1 cut(s) 150
DpnI GATC 2 cut(s) 29, 77
DpnII GATC 2 cut(s) 27, 75
Eco88I CYCGRG 1 cut(s) 232
Fnu4HI GCNGC 3 cut(s) 6, 216, 248
FokI GGATG 1 cut(s) 263
Fsp4HI GCNGC 3 cut(s) 6, 216, 248
FspBI CTAG 2 cut(s) 11, 210
GlaI GCGC 1 cut(s) 226
GluI GCNGC 3 cut(s) 6, 216, 248
HaeIII GGCC 1 cut(s) 68
HapII CCGG 1 cut(s) 41
HgaI GACGC 3 cut(s) 37, 83, 239
HhaI GCGC 1 cut(s) 227
Hin1I GRCGYC 2 cut(s) 48, 250
Hin6I GCGC 1 cut(s) 225
HinP1I GCGC 1 cut(s) 225
HinfI GANTC 2 cut(s) 155, 172
HpaII CCGG 1 cut(s) 41
HphI GGTGA 1 cut(s) 43
Hpy188I TCNGA 3 cut(s) 25, 99, 287
Hpy188III TCNNGA 1 cut(s) 178
Hpy99I CGWCG 1 cut(s) 99
HpyCH4IV ACGT 1 cut(s) 72
HpyF10VI GCNNNNNNNGC 2 cut(s) 224, 253
HpyF3I CTNAG 1 cut(s) 150
HpySE526I ACGT 1 cut(s) 72
Hsp92I GRCGYC 2 cut(s) 48, 250
HspAI GCGC 1 cut(s) 225
Kzo9I GATC 2 cut(s) 27, 75
LpnPI CCDG 2 cut(s) 54, 186
MaeI CTAG 2 cut(s) 11, 210
MaeII ACGT 1 cut(s) 72
MaeIII GTNAC 2 cut(s) 49, 145
MalI GATC 2 cut(s) 29, 77
MbiI CCGCTC 1 cut(s) 247
MboI GATC 2 cut(s) 27, 75
MboII GAAGA 1 cut(s) 181
MluCI AATT 1 cut(s) 127
MlyI GAGTC 1 cut(s) 164
MmeI TCCRAC 2 cut(s) 77, 183
MnlI CCTC 7 cut(s) 31, 57, 145, 178, 238, 241, 248
MspI CCGG 1 cut(s) 41
MspR9I CCNGG 1 cut(s) 41
MwoI GCNNNNNNNGC 2 cut(s) 224, 253
NciI CCSGG 1 cut(s) 41
NdeII GATC 2 cut(s) 27, 75
NmuCI GTSAC 2 cut(s) 49, 145
NspV TTCGAA 1 cut(s) 138
PaeR7I CTCGAG 1 cut(s) 232
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PfeI GAWTC 1 cut(s) 172
PflFI GACNNNGTC 1 cut(s) 47
PkrI GCNGC 3 cut(s) 7, 217, 249
PleI GAGTC 1 cut(s) 163
PpsI GAGTC 1 cut(s) 163
PspPI GGNCC 1 cut(s) 66
PspXI VCTCGAGB 1 cut(s) 232
PsyI GACNNNGTC 1 cut(s) 47
SatI GCNGC 3 cut(s) 6, 216, 248
Sau3AI GATC 2 cut(s) 27, 75
Sau96I GGNCC 1 cut(s) 66
SchI GAGTC 1 cut(s) 164
ScrFI CCNGG 1 cut(s) 41
SetI ASST 2 cut(s) 75, 285
Sfr274I CTCGAG 1 cut(s) 232
SfuI TTCGAA 1 cut(s) 138
SlaI CTCGAG 1 cut(s) 232
SmlI CTYRAG 2 cut(s) 176, 232
SmoI CTYRAG 2 cut(s) 176, 232
Sse9I AATT 1 cut(s) 127
SsiI CCGC 5 cut(s) 5, 216, 247, 254, 272
SspMI CTAG 2 cut(s) 11, 210
StyD4I CCNGG 1 cut(s) 39
TaiI ACGT 1 cut(s) 75
TaqI TCGA 6 cut(s) 78, 84, 117, 132, 138, 233
TasI AATT 1 cut(s) 127
TauI GCSGC 3 cut(s) 8, 218, 250
TfiI GAWTC 1 cut(s) 172
TscAI CASTG 1 cut(s) 154
TseFI GTSAC 2 cut(s) 49, 145
Tsp45I GTSAC 2 cut(s) 49, 145
TspDTI ATGAA 1 cut(s) 253
TspGWI ACGGA 1 cut(s) 230
TspRI CASTG 1 cut(s) 154
Tth111I GACNNNGTC 1 cut(s) 47
XhoI CTCGAG 1 cut(s) 232
XspI CTAG 2 cut(s) 11, 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.