MD03G1156900.v1.1

Blue copper protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0005440
Physical Location & Seq
Forward (+)
18484938 .. 18488935
3998 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1156900.v1.1.491

Sequence Viewer

Length: 738 bp
ATGGAGTTATTAATATTCCTACAGAGAAAGTCCAGCTGCAGCAGCTTGTGTTTTCTTCTTCTTCTTCTTCCTCTTGTGTTTTCGTTTTCAGGAATATCAGTTGTTGAAGGTTACAAGAATTACACAGTGGGGGACTCTTCCGGTTGGTACGCAGCTGTTAACTATCAGAAATGGGCTGACGCCAAGACATTCAGCTTGGGAGATTTTCTAATTTTTAATACGGACACCAACCACTCGGTTGTGCAAACATATAACTTGACAACCTACAAGCTGTGCGATTACAACGATGCTCTAGAAAACGATACGATGCAATGGTCGGCCGCGGACCCGTCAAACATGGCCATACATGACGTCTCAGTGGCAGTGCCTTTGGTAAGAGAAGGGATGAACTATTTCTTTTCAGGGGATTATGATGGCGAGCAATGCAAGAACGGACAGCACTTTAAGATCACCGTCTCTCACGGACAAGGTTTGCCAAAGAGTTTACAGAACCCAGCCGTTCAGTCCCCAAGCCCAGCAAGCGGTCCCGGCCGGCAGTCTGCAGGTGATGGCGACAATGATGATGGTTCTGTCCCAGATACCATTGTTCCCGCCAGTTTCGACCATCCCAAAGAGGATGACACTGCCACTGGCGATCATGATAAAGATTCATCTGGATCACTTTGTGTGAAGCACAAGCACGGGTTGCTATTTAGGGTTTTCATTTTTTTAGGGTTTCTCTACTGCTGCCTCTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

246

Amino Acids

26.94

Weight (kDa)

4.87

Isoelectric Point (pI)

31.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 51 - 146 1.4e-16 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 533
AatII GACGTC 1 cut(s) 354
Acc36I ACCTGC 1 cut(s) 533
AccII CGCG 1 cut(s) 323
AciI CCGC 4 cut(s) 321, 323, 522, 591
AclWI GGATC 1 cut(s) 664
AcoI YGGCCR 3 cut(s) 318, 339, 529
AcyI GRCGYC 2 cut(s) 180, 351
AdeI CACNNNGTG 1 cut(s) 665
AfaI GTAC 1 cut(s) 149
AfiI CCNNNNNNNGG 1 cut(s) 521
AgsI TTSAA 1 cut(s) 107
AluBI AGCT 5 cut(s) 36, 45, 155, 195, 271
AluI AGCT 5 cut(s) 36, 45, 155, 195, 271
Alw26I GTCTC 2 cut(s) 358, 460
AlwI GGATC 1 cut(s) 664
AoxI GGCC 3 cut(s) 318, 339, 529
ApeKI GCWGC 5 cut(s) 36, 39, 42, 152, 726
AseI ATTAAT 1 cut(s) 11
Asp700I GAANNNNTTC 1 cut(s) 392
AspS9I GGNCC 2 cut(s) 325, 524
AsuC2I CCSGG 1 cut(s) 528
AsuHPI GGTGA 2 cut(s) 442, 557
AvaII GGWCC 2 cut(s) 325, 524
BaeI ACNNNNGTAYC 2 cut(s) 570, 603
BalI TGGCCA 1 cut(s) 341
BbvI GCAGC 5 cut(s) 23, 51, 54, 164, 713
BccI CCATC 4 cut(s) 407, 542, 557, 612
BceAI ACGGC 1 cut(s) 482
BcnI CCSGG 1 cut(s) 528
BcoDI GTCTC 2 cut(s) 358, 460
BfaI CTAG 1 cut(s) 293
BfmI CTRYAG 3 cut(s) 20, 37, 540
BfuAI ACCTGC 1 cut(s) 533
BisI GCNGC 6 cut(s) 37, 40, 43, 153, 321, 727
BlsI GCNGC 6 cut(s) 38, 41, 44, 154, 322, 728
Bme1390I CCNGG 1 cut(s) 528
Bme18I GGWCC 2 cut(s) 325, 524
BmgT120I GGNCC 2 cut(s) 325, 524
BmiI GGNNCC 2 cut(s) 327, 526
BmrFI CCNGG 1 cut(s) 528
BmsI GCATC 2 cut(s) 277, 297
BpuMI CCSGG 1 cut(s) 528
BsaHI GRCGYC 2 cut(s) 180, 351
BsaJI CCNNGG 1 cut(s) 321
BsaWI WCCGGW 1 cut(s) 140
Bsc4I CCNNNNNNNGG 1 cut(s) 521
Bse118I RCCGGY 1 cut(s) 531
Bse1I ACTGG 2 cut(s) 594, 634
Bse3DI GCAATG 2 cut(s) 317, 428
BseDI CCNNGG 1 cut(s) 321
BseGI GGATG 3 cut(s) 390, 604, 622
BseLI CCNNNNNNNGG 1 cut(s) 521
BseMI GCAATG 2 cut(s) 317, 428
BseMII CTCAG 1 cut(s) 369
BseNI ACTGG 2 cut(s) 594, 634
BseX3I CGGCCG 2 cut(s) 318, 529
BseXI GCAGC 5 cut(s) 23, 51, 54, 164, 713
BseYI CCCAGC 2 cut(s) 493, 514
Bsh1236I CGCG 1 cut(s) 323
Bsh1285I CGRYCG 2 cut(s) 321, 532
BshFI GGCC 3 cut(s) 320, 341, 531
BsiEI CGRYCG 2 cut(s) 321, 532
BsiSI CCGG 3 cut(s) 141, 528, 532
BslFI GGGAC 4 cut(s) 146, 490, 510, 557
BslI CCNNNNNNNGG 1 cut(s) 521
BsmAI GTCTC 2 cut(s) 358, 460
BsmBI CGTCTC 2 cut(s) 358, 460
BsmFI GGGAC 4 cut(s) 146, 490, 510, 557
BsnI GGCC 3 cut(s) 320, 341, 531
Bsp143I GATC 3 cut(s) 447, 634, 656
BspACI CCGC 4 cut(s) 321, 323, 522, 591
BspANI GGCC 3 cut(s) 320, 341, 531
BspCNI CTCAG 1 cut(s) 368
BspFNI CGCG 1 cut(s) 323
BspHI TCATGA 1 cut(s) 637
BspLI GGNNCC 2 cut(s) 327, 526
BspMAI CTGCAG 2 cut(s) 41, 544
BspMI ACCTGC 1 cut(s) 533
BspPI GGATC 1 cut(s) 664
BsrDI GCAATG 2 cut(s) 317, 428
BsrFI RCCGGY 1 cut(s) 531
BsrI ACTGG 2 cut(s) 594, 634
BssAI RCCGGY 1 cut(s) 531
BssECI CCNNGG 1 cut(s) 321
BssMI GATC 3 cut(s) 447, 634, 656
BssNI GRCGYC 2 cut(s) 180, 351
Bst4CI ACNGT 2 cut(s) 127, 454
Bst6I CTCTTC 1 cut(s) 142
BstACI GRCGYC 2 cut(s) 180, 351
BstAPI GCANNNNNTGC 1 cut(s) 685
BstC8I GCNNGC 3 cut(s) 419, 520, 533
BstDEI CTNAG 1 cut(s) 355
BstDSI CCRYGG 1 cut(s) 321
BstF5I GGATG 3 cut(s) 390, 604, 622
BstFNI CGCG 1 cut(s) 323
BstKTI GATC 3 cut(s) 450, 637, 659
BstMAI GTCTC 2 cut(s) 358, 460
BstMBI GATC 3 cut(s) 447, 634, 656
BstMCI CGRYCG 2 cut(s) 321, 532
BstMWI GCNNNNNNNGC 5 cut(s) 42, 423, 519, 528, 685
BstSCI CCNGG 1 cut(s) 526
BstSFI CTRYAG 3 cut(s) 20, 37, 540
BstUI CGCG 1 cut(s) 323
BstV1I GCAGC 5 cut(s) 23, 51, 54, 164, 713
BstZI CGGCCG 2 cut(s) 318, 529
BsuRI GGCC 3 cut(s) 320, 341, 531
BtgI CCRYGG 1 cut(s) 321
BtsCI GGATG 3 cut(s) 390, 604, 622
BtsI GCAGTG 2 cut(s) 369, 621
BtsIMutI CAGTG 5 cut(s) 132, 363, 369, 621, 627
BveI ACCTGC 1 cut(s) 533
Cac8I GCNNGC 3 cut(s) 419, 520, 533
CciI TCATGA 1 cut(s) 637
Cfr10I RCCGGY 1 cut(s) 531
Cfr13I GGNCC 2 cut(s) 325, 524
Cfr42I CCGCGG 1 cut(s) 324
CseI GACGC 1 cut(s) 188
Csp6I GTAC 1 cut(s) 148
CviAII CATG 3 cut(s) 337, 347, 638
CviQI GTAC 1 cut(s) 148
DdeI CTNAG 1 cut(s) 355
DpnI GATC 3 cut(s) 449, 636, 658
DpnII GATC 3 cut(s) 447, 634, 656
DraIII CACNNNGTG 1 cut(s) 665
EaeI YGGCCR 3 cut(s) 318, 339, 529
EagI CGGCCG 2 cut(s) 318, 529
Eam1104I CTCTTC 1 cut(s) 142
EarI CTCTTC 1 cut(s) 142
EclXI CGGCCG 2 cut(s) 318, 529
Eco47I GGWCC 2 cut(s) 325, 524
Eco52I CGGCCG 2 cut(s) 318, 529
Esp3I CGTCTC 2 cut(s) 358, 460
FaeI CATG 3 cut(s) 340, 350, 641
FaiI YATR 7 cut(s) 250, 252, 338, 344, 348, 411, 639
FaqI GGGAC 4 cut(s) 146, 490, 510, 557
FatI CATG 3 cut(s) 336, 346, 637
FauI CCCGC 1 cut(s) 598
Fnu4HI GCNGC 6 cut(s) 37, 40, 43, 153, 321, 727
FokI GGATG 3 cut(s) 397, 591, 629
Fsp4HI GCNGC 6 cut(s) 37, 40, 43, 153, 321, 727
FspBI CTAG 1 cut(s) 293
GluI GCNGC 6 cut(s) 37, 40, 43, 153, 321, 727
GsaI CCCAGC 2 cut(s) 497, 518
HaeIII GGCC 3 cut(s) 320, 341, 531
HapII CCGG 3 cut(s) 141, 528, 532
HgaI GACGC 1 cut(s) 188
Hin1I GRCGYC 2 cut(s) 180, 351
Hin1II CATG 3 cut(s) 340, 350, 641
HincII GTYRAC 1 cut(s) 160
HindII GTYRAC 1 cut(s) 160
HinfI GANTC 2 cut(s) 134, 647
HpaI GTTAAC 1 cut(s) 160
HpaII CCGG 3 cut(s) 141, 528, 532
HphI GGTGA 2 cut(s) 442, 557
Hpy166II GTNNAC 2 cut(s) 160, 485
Hpy188I TCNGA 1 cut(s) 168
Hpy188III TCNNGA 4 cut(s) 90, 293, 638, 654
Hpy8I GTNNAC 2 cut(s) 160, 485
HpyAV CCTTC 2 cut(s) 101, 374
HpyCH4III ACNGT 2 cut(s) 127, 454
HpyCH4IV ACGT 1 cut(s) 351
HpyCH4V TGCA 5 cut(s) 39, 244, 310, 426, 542
HpyF10VI GCNNNNNNNGC 5 cut(s) 42, 423, 519, 528, 685
HpyF3I CTNAG 1 cut(s) 355
HpySE526I ACGT 1 cut(s) 351
Hsp92I GRCGYC 2 cut(s) 180, 351
Hsp92II CATG 3 cut(s) 340, 350, 641
KroI GCCGGC 1 cut(s) 531
KroNI GCCGGC 1 cut(s) 533
KspAI GTTAAC 1 cut(s) 160
KspI CCGCGG 1 cut(s) 324
Kzo9I GATC 3 cut(s) 447, 634, 656
Lsp1109I GCAGC 5 cut(s) 23, 51, 54, 164, 713
LweI GCATC 2 cut(s) 277, 297
MaeI CTAG 1 cut(s) 293
MaeII ACGT 1 cut(s) 351
MaeIII GTNAC 1 cut(s) 110
MalI GATC 3 cut(s) 449, 636, 658
MboI GATC 3 cut(s) 447, 634, 656
MboII GAAGA 6 cut(s) 47, 50, 53, 56, 59, 129
MlsI TGGCCA 1 cut(s) 341
MluCI AATT 2 cut(s) 118, 210
MluNI TGGCCA 1 cut(s) 341
MlyI GAGTC 1 cut(s) 128
MnlI CCTC 2 cut(s) 81, 607
Mox20I TGGCCA 1 cut(s) 341
MroNI GCCGGC 1 cut(s) 531
MroXI GAANNNNTTC 1 cut(s) 392
MscI TGGCCA 1 cut(s) 341
MseI TTAA 4 cut(s) 11, 159, 216, 444
Msp20I TGGCCA 1 cut(s) 341
MspA1I CMGCKG 3 cut(s) 36, 155, 323
MspI CCGG 3 cut(s) 141, 528, 532
MspR9I CCNGG 1 cut(s) 528
MvnI CGCG 1 cut(s) 323
MwoI GCNNNNNNNGC 5 cut(s) 42, 423, 519, 528, 685
NaeI GCCGGC 1 cut(s) 533
NciI CCSGG 1 cut(s) 528
NdeII GATC 3 cut(s) 447, 634, 656
NgoMIV GCCGGC 1 cut(s) 531
NlaIII CATG 3 cut(s) 340, 350, 641
NlaIV GGNNCC 2 cut(s) 327, 526
PagI TCATGA 1 cut(s) 637
PaqCI CACCTGC 1 cut(s) 533
PdiI GCCGGC 1 cut(s) 533
PdmI GAANNNNTTC 1 cut(s) 392
PfeI GAWTC 1 cut(s) 647
PkrI GCNGC 6 cut(s) 38, 41, 44, 154, 322, 728
PleI GAGTC 1 cut(s) 128
PpsI GAGTC 1 cut(s) 128
PshBI ATTAAT 1 cut(s) 11
PspFI CCCAGC 2 cut(s) 493, 514
PspN4I GGNNCC 2 cut(s) 327, 526
PspPI GGNCC 2 cut(s) 325, 524
PstI CTGCAG 2 cut(s) 41, 544
PvuII CAGCTG 2 cut(s) 36, 155
RsaI GTAC 1 cut(s) 149
RsaNI GTAC 1 cut(s) 148
SacII CCGCGG 1 cut(s) 324
SaqAI TTAA 4 cut(s) 11, 159, 216, 444
SatI GCNGC 6 cut(s) 37, 40, 43, 153, 321, 727
Sau3AI GATC 3 cut(s) 447, 634, 656
Sau96I GGNCC 2 cut(s) 325, 524
SchI GAGTC 1 cut(s) 128
ScrFI CCNGG 1 cut(s) 528
SfaNI GCATC 2 cut(s) 277, 297
SfcI CTRYAG 3 cut(s) 20, 37, 540
Sfr303I CCGCGG 1 cut(s) 324
SgrBI CCGCGG 1 cut(s) 324
SinI GGWCC 2 cut(s) 325, 524
Sse9I AATT 2 cut(s) 118, 210
SsiI CCGC 4 cut(s) 321, 323, 522, 591
SspI AATATT 1 cut(s) 15
SspMI CTAG 1 cut(s) 293
StyD4I CCNGG 1 cut(s) 526
TaaI ACNGT 2 cut(s) 127, 454
TaiI ACGT 1 cut(s) 354
TaqI TCGA 1 cut(s) 600
TasI AATT 2 cut(s) 118, 210
TauI GCSGC 1 cut(s) 323
TfiI GAWTC 1 cut(s) 647
Tru1I TTAA 4 cut(s) 11, 159, 216, 444
Tru9I TTAA 4 cut(s) 11, 159, 216, 444
TscAI CASTG 5 cut(s) 132, 363, 369, 628, 634
TseI GCWGC 5 cut(s) 36, 39, 42, 152, 726
TspDTI ATGAA 3 cut(s) 401, 639, 691
TspGWI ACGGA 3 cut(s) 236, 447, 477
TspRI CASTG 5 cut(s) 132, 363, 369, 628, 634
VpaK11BI GGWCC 2 cut(s) 325, 524
VspI ATTAAT 1 cut(s) 11
XbaI TCTAGA 1 cut(s) 292
XmnI GAANNNNTTC 1 cut(s) 392
XspI CTAG 1 cut(s) 293
ZraI GACGTC 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.