FvH4_5g25080

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004858
Physical Location & Seq
Forward (+)
16562223 .. 16567717
5495 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g25080.t1

Sequence Viewer

Length: 2583 bp
ATGTCCGGGGAGGCGCGTCGCCTGCACCCTTCCCTCCCCAAGGTACACTATCACACTCTCCACTCCCGACCCACCTCTGGCTTTGATTATCTCACTCAGTTCTTACCATTTTCCTCTAATAAACCCAACTCCAAGACCTCTCTAAACTCCAATGAGAGGCGTAAAGTCACTGTTGGGTTATCAAAGATTATAAAGAACCAACATGGCTATGTCTTGAGGGGATTTTCCAGAAGCTTCTGCCCATTTTTTCTTGTAAAGATTATGAAACTCTTGTGTTGTAGAGAAACTGCATTTGGGTTCTTTAAATTGGCCTTTAGAGATGATAGTCAGGACACTGTGGAGTCGTGTTGTGTGGCTTCTCATGTTTTGGCAGCCGAAAATTTACGGTTTTTGGCTCAGGATGTGGTTTCTTGTGTGATTGGGAGGATTGGGCCTAGTAGGAGTAGGGATTTGGTGAGGTGTATGTGGGGAGGGCATTGTGGATATGAGTCGGATTTTTCGGTTCTTGATACGTTAATGAGGGGGTTCTTGAATGTGGAAATGGGTTTCGAGGCTTTGGAGGTTGTGAGGAGGATGAGGGAGGTGGGAGTGAGGCCGAGTTTGTCAGCGGTTGCGATTCTGTTCAGGTTGTTGATAAGGGTTGGTGATTATGGGAGTGTGTGGAAGGTGTTTAGAGATATGATTAGAATGGGGCCTTATCCGTGTAATTACATTTATAGCACAATGATTCTTGCGTTTTGTCGAAAAGGGTTGCTTAGAGTTGGAGAGAGTTTGTTGCAGGTGATGTGGAAGTTCCGGTGTGAACCGGATGTTTTTTCTTATAACATTGTGATCTATGCAAATTGTGTGAGGGGGCAGACAGCTGATGCACTTGAGTGGGTGGAGTTGATGATTGCGAGAGGATGTAGACCGAGCATTGTTACATTTAATACAGTGATTAGTGCATTGTGTAACAAAGGAAATATGGTGGAGGCAAGGAAGCTTTTTGATGAGGTCCCATACATGGGTGTTTCTCCAAACACCGTAATGTACAATACCATGATGGATGGGTATGTTAAGGCAAGGGATGTTGGTCACGCTAACATGCTTTTTGAAGAAATGAGGGACAATAGTATTTCTCCAGACTGCATAACTTTCAATATTTTGATCGGAGGGCATTTCAAGTATGGGAGGGAAGATGATGGAGACAGGTTGTTGCGGGACTTATCTGTGTCAGGATTACTTCCAGATTCTTCGTTGTATGATATTTATGTTTCTTGGTTGTGTTGGGCAGATCGGTTGGACGATGCCATGGCAGTTTTAGAGGATATGCTTGAAAGAGGATTGCCTCTAAGTGTGGTCGCTTTTAATTCTGTTATTGCAGCTTGTAGCAGAGTAGGGTTAGAAGAGAAAGCATATAAGGCCTTTAATTTTATGATTTCATTTGGTATAACTCCTTCATCATCTACATGGAGTTCTTTGCTTATGGGTTTATCTAATAAGGGGTACTTGCAAGAAGCCAGAGAGCTTTTGTGTAAGATGATGGAGAAGGGGTATCCTATCAAAAAAGCTGCTTTTACGGTACTTTTTGATGGGTACTTTAGGATGGGTGATTTAGATCGGGCACAAAGTTTGTGGAATGAGATGGAAGGAAGAGGTATATCTCCTGATGTTGTTGCCTTTTCAGCATTAATCAATGGACTATCTAAAGCCGGTTTAATGGAAGAAGCATGTGATATGTTTCAAGTAATGTCAAGGAAAGGCTTTGTGCCCAATAATTTTGTGTATAATTCTTTGATTGGCGGCTTTTGCCACTGCGATAAGTTAAGTGATGCAATGAGGTTGGAGAAAGAGATGAGGCAAAAGGGTCTTGTTCCAGATATCTTTACTACCAATATGATCATTCATGGGTTCTGTAGACAGGGGAGGATGAAGTCAGCAATGAATATATTTATGAAAATGTTCCAAACTGGTTTAACCCCAGATATTGTCACATACAATACACTAATTGGTGGTTATTGTAAAGCATTTGACATGGTTAGGGCAGATGAGATTTTGTATAAAATGTACGCTAGTGGATGGGAACCAGATATCACAACCTATAATATACGCATGCATGGATTTTGTAGTACCCGAAAGATGAATCGAGCTGTGATGATAATGGATGAGCTTATATCAAGGGGTGTTGTTCCAGACACAGTAACATACAACACTATGATGAATGGTGTTTGTGTTGAGATACTGGATCGTGCTATGATTTTAACTGCTAAATTGCTTAAATTGGCGTTTCTTCCAAATGTTGTTACAGCTAATGTATTGTTGTCTCAGTTCCGCAGGCAGGGGATGCCTGAGAAGGCCCTATTATGGGGTCAGAAGTTGAGTCAGTTTTCCGTCTGTTTTGATGAAATTACCTACAAAATATTGGACAGAGCATATCATAATATGCAAGAAGATCCTGAAACTTCAAGGGCAACACCTGAGAAAAGCCTCTTCCTGGATTTCCTCATGTACATTACATATGATTATTTATGTAGAAATAAACCTCGCAGAGATGTAAGACAAGACCCTCTAGAAGTAATTGGTTTTAGTGGCCCTGAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 map00500
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

861

Amino Acids

97.77

Weight (kDa)

8.46

Isoelectric Point (pI)

38.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 234 - 282 1.7e-07 PPR repeat family
PPR_3 PF13812 267 - 317 2.1e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 269 - 309 1.6e-06 PPR repeat family
PPR_1 PF12854 300 - 331 2e-10 PPR repeat
PPR_2 PF13041 304 - 342 2.7e-09 PPR repeat family
PPR PF01535 307 - 336 2.9e-06 PPR repeat
PPR_3 PF13812 327 - 384 2.5e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 335 - 368 7.5e-07 PPR repeat
PPR_2 PF13041 339 - 387 6.1e-16 PPR repeat family
PPR PF01535 342 - 371 6.1e-07 PPR repeat
PPR_long PF17177 428 - 565 1.6e-08 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 430 - 541 5.8e-08 Fungal tetratrico peptide repeats
PPR_2 PF13041 445 - 492 6.5e-08 PPR repeat family
PPR_2 PF13041 479 - 526 5.1e-06 PPR repeat family
PPR_2 PF13041 518 - 563 6.9e-12 PPR repeat family
PPR_3 PF13812 538 - 595 7e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 545 - 577 1.2e-08 PPR repeat
PPR_2 PF13041 549 - 598 1.2e-14 PPR repeat family
PPR PF01535 552 - 582 9.4e-07 PPR repeat
PPR_3 PF13812 574 - 628 6.1e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 580 - 613 5.8e-08 PPR repeat
PPR_2 PF13041 584 - 633 5e-14 PPR repeat family
PPR PF01535 588 - 617 6.4e-06 PPR repeat
PPR_3 PF13812 608 - 666 4.3e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 615 - 647 8.9e-09 PPR repeat
PPR_2 PF13041 619 - 668 1.2e-16 PPR repeat family
PPR_1 PF12854 651 - 675 1.4e-07 PPR repeat
PPR_2 PF13041 654 - 702 1e-13 PPR repeat family
PPR_3 PF13812 678 - 734 1e-05 Pentatricopeptide repeat domain
PPR_2 PF13041 689 - 737 5e-14 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 191, 822
AarI CACCTGC 1 cut(s) 769
Acc36I ACCTGC 1 cut(s) 769
AccI GTMKAC 2 cut(s) 907, 1897
AccII CGCG 1 cut(s) 16
AciI CCGC 4 cut(s) 608, 1198, 1782, 2311
AclWI GGATC 2 cut(s) 2232, 2426
AcsI RAATTY 1 cut(s) 379
AfaI GTAC 8 cut(s) 45, 1031, 1487, 1563, 1577, 2048, 2110, 2489
AfiI CCNNNNNNNGG 9 cut(s) 40, 77, 156, 1003, 1004, 2317, 2343, 2344, 2473
AgsI TTSAA 7 cut(s) 532, 1094, 1138, 1162, 1316, 1724, 2445
AjnI CCWGG 1 cut(s) 2472
AjuI GAANNNNNNNTTGG 2 cut(s) 276, 308
AleI CACNNNNGTG 1 cut(s) 874
AluBI AGCT 9 cut(s) 234, 863, 982, 1364, 1507, 1550, 2129, 2149, 2288
AluI AGCT 9 cut(s) 234, 863, 982, 1364, 1507, 1550, 2129, 2149, 2288
Alw26I GTCTC 2 cut(s) 1179, 2307
AlwI GGATC 2 cut(s) 2232, 2426
AoxI GGCC 7 cut(s) 309, 431, 593, 692, 1401, 2334, 2569
ApeKI GCWGC 3 cut(s) 371, 1361, 1550
ApoI RAATTY 1 cut(s) 379
AseI ATTAAT 1 cut(s) 1670
Asp700I GAANNNNTTC 1 cut(s) 1940
AspLEI GCGC 1 cut(s) 16
AspS9I GGNCC 5 cut(s) 431, 692, 994, 2335, 2570
AsuC2I CCSGG 1 cut(s) 7
AsuHPI GGTGA 4 cut(s) 466, 656, 793, 1601
AvaII GGWCC 1 cut(s) 994
BaeGI GKGCMC 2 cut(s) 1606, 1752
BarI GAAGNNNNNNTAC 2 cut(s) 1420, 1452
BbvI GCAGC 3 cut(s) 383, 1373, 1537
BccI CCATC 8 cut(s) 1036, 1040, 1175, 1516, 1565, 1579, 1618, 2052
BciT130I CCWGG 1 cut(s) 2474
BciVI GTATCC 1 cut(s) 1545
BclI TGATCA 1 cut(s) 1878
BcnI CCSGG 1 cut(s) 7
BcoDI GTCTC 2 cut(s) 1179, 2307
BfaI CTAG 3 cut(s) 435, 2052, 2549
BfmI CTRYAG 1 cut(s) 1894
BfuAI ACCTGC 1 cut(s) 769
BfuI GTATCC 1 cut(s) 1545
BisI GCNGC 4 cut(s) 372, 1362, 1551, 1783
BlsI GCNGC 4 cut(s) 373, 1363, 1552, 1784
Bme1390I CCNGG 2 cut(s) 7, 2474
Bme18I GGWCC 1 cut(s) 994
BmgT120I GGNCC 5 cut(s) 431, 692, 994, 2335, 2570
BmiI GGNNCC 3 cut(s) 693, 996, 2064
BmrFI CCNGG 2 cut(s) 7, 2474
BmsI GCATC 4 cut(s) 856, 1276, 1801, 2313
BpmI CTGGAG 1 cut(s) 1104
Bpu10I CCTNAGC 1 cut(s) 396
BpuEI CTTGAG 2 cut(s) 235, 893
BpuMI CCSGG 1 cut(s) 7
BsaBI GATNNNNATC 1 cut(s) 1596
BsaJI CCNNGG 3 cut(s) 6, 39, 1290
BsaWI WCCGGW 2 cut(s) 795, 805
BsaXI ACNNNNNCTCC 2 cut(s) 1517, 1547
Bsc4I CCNNNNNNNGG 9 cut(s) 40, 77, 156, 1003, 1004, 2317, 2343, 2344, 2473
Bse118I RCCGGY 1 cut(s) 1691
Bse1I ACTGG 2 cut(s) 1954, 2226
Bse3DI GCAATG 2 cut(s) 1821, 1926
Bse8I GATNNNNATC 1 cut(s) 1596
BseBI CCWGG 1 cut(s) 2474
BseDI CCNNGG 3 cut(s) 6, 39, 1290
BseJI GATNNNNATC 1 cut(s) 1596
BseLI CCNNNNNNNGG 9 cut(s) 40, 77, 156, 1003, 1004, 2317, 2343, 2344, 2473
BseMI GCAATG 2 cut(s) 1821, 1926
BseMII CTCAG 5 cut(s) 110, 410, 2318, 2319, 2448
BseNI ACTGG 2 cut(s) 1954, 2226
BseRI GAGGAG 1 cut(s) 583
BseSI GKGCMC 2 cut(s) 1606, 1752
BseXI GCAGC 3 cut(s) 383, 1373, 1537
BsgI GTGCAG 1 cut(s) 8
Bsh1236I CGCG 1 cut(s) 16
BshFI GGCC 7 cut(s) 311, 433, 595, 694, 1403, 2336, 2571
BsiSI CCGG 4 cut(s) 6, 796, 806, 1692
BslFI GGGAC 3 cut(s) 980, 1118, 1214
BslI CCNNNNNNNGG 9 cut(s) 40, 77, 156, 1003, 1004, 2317, 2343, 2344, 2473
BsmAI GTCTC 2 cut(s) 1179, 2307
BsmFI GGGAC 3 cut(s) 980, 1118, 1214
BsnI GGCC 7 cut(s) 311, 433, 595, 694, 1403, 2336, 2571
Bsp1286I GDGCHC 2 cut(s) 1606, 1752
Bsp1407I TGTACA 2 cut(s) 1029, 2487
Bsp143I GATC 7 cut(s) 831, 1146, 1273, 1597, 1878, 2224, 2431
Bsp19I CCATGG 1 cut(s) 1290
BspACI CCGC 4 cut(s) 608, 1198, 1782, 2311
BspANI GGCC 7 cut(s) 311, 433, 595, 694, 1403, 2336, 2571
BspCNI CTCAG 5 cut(s) 109, 409, 2317, 2320, 2449
BspFNI CGCG 1 cut(s) 16
BspLI GGNNCC 3 cut(s) 693, 996, 2064
BspMI ACCTGC 1 cut(s) 769
BspPI GGATC 2 cut(s) 2232, 2426
BsrDI GCAATG 2 cut(s) 1821, 1926
BsrFI RCCGGY 1 cut(s) 1691
BsrGI TGTACA 2 cut(s) 1029, 2487
BsrI ACTGG 2 cut(s) 1954, 2226
BssAI RCCGGY 1 cut(s) 1691
BssECI CCNNGG 3 cut(s) 6, 39, 1290
BssMI GATC 7 cut(s) 831, 1146, 1273, 1597, 1878, 2224, 2431
BssT1I CCWWGG 2 cut(s) 39, 1290
Bst2UI CCWGG 1 cut(s) 2474
Bst4CI ACNGT 7 cut(s) 172, 337, 387, 934, 1024, 1561, 2179
Bst6I CTCTTC 3 cut(s) 1380, 1627, 2474
BstAPI GCANNNNNTGC 1 cut(s) 2323
BstAUI TGTACA 2 cut(s) 1029, 2487
BstC8I GCNNGC 3 cut(s) 23, 2093, 2315
BstDEI CTNAG 7 cut(s) 96, 396, 755, 1331, 2304, 2328, 2457
BstDSI CCRYGG 1 cut(s) 1290
BstENI CCTNNNNNAGG 1 cut(s) 38
BstFNI CGCG 1 cut(s) 16
BstHHI GCGC 1 cut(s) 16
BstKTI GATC 7 cut(s) 834, 1149, 1276, 1600, 1881, 2227, 2434
BstMAI GTCTC 2 cut(s) 1179, 2307
BstMBI GATC 7 cut(s) 831, 1146, 1273, 1597, 1878, 2224, 2431
BstMWI GCNNNNNNNGC 5 cut(s) 22, 1400, 1664, 1788, 2323
BstNI CCWGG 1 cut(s) 2474
BstNSI RCATGY 3 cut(s) 1087, 1713, 2095
BstSCI CCNGG 2 cut(s) 5, 2472
BstSFI CTRYAG 1 cut(s) 1894
BstSLI GKGCMC 2 cut(s) 1606, 1752
BstUI CGCG 1 cut(s) 16
BstV1I GCAGC 3 cut(s) 383, 1373, 1537
BstX2I RGATCY 1 cut(s) 2431
BstYI RGATCY 1 cut(s) 2431
BsuI GTATCC 1 cut(s) 1545
BsuRI GGCC 7 cut(s) 311, 433, 595, 694, 1403, 2336, 2571
BtgI CCRYGG 1 cut(s) 1290
BtsI GCAGTG 1 cut(s) 1792
BtsIMutI CAGTG 4 cut(s) 168, 333, 939, 1792
BveI ACCTGC 1 cut(s) 769
Cac8I GCNNGC 3 cut(s) 23, 2093, 2315
CfoI GCGC 1 cut(s) 16
Cfr10I RCCGGY 1 cut(s) 1691
Cfr13I GGNCC 5 cut(s) 431, 692, 994, 2335, 2570
CseI GACGC 1 cut(s) 5
Csp6I GTAC 8 cut(s) 44, 1030, 1486, 1562, 1576, 2047, 2109, 2488
CspCI CAANNNNNGTGG 2 cut(s) 1595, 1630
CviQI GTAC 8 cut(s) 44, 1030, 1486, 1562, 1576, 2047, 2109, 2488
DdeI CTNAG 7 cut(s) 96, 396, 755, 1331, 2304, 2328, 2457
DpnI GATC 7 cut(s) 833, 1148, 1275, 1599, 1880, 2226, 2433
DpnII GATC 7 cut(s) 831, 1146, 1273, 1597, 1878, 2224, 2431
DraI TTTAAA 1 cut(s) 304
Eam1104I CTCTTC 3 cut(s) 1380, 1627, 2474
EarI CTCTTC 3 cut(s) 1380, 1627, 2474
Eco130I CCWWGG 2 cut(s) 39, 1290
Eco147I AGGCCT 1 cut(s) 1403
Eco32I GATATC 2 cut(s) 1861, 2071
Eco47I GGWCC 1 cut(s) 994
EcoNI CCTNNNNNAGG 1 cut(s) 38
EcoO109I RGGNCCY 3 cut(s) 692, 994, 2335
EcoRII CCWGG 1 cut(s) 2472
EcoRV GATATC 2 cut(s) 1861, 2071
EcoT14I CCWWGG 2 cut(s) 39, 1290
EcoT22I ATGCAT 1 cut(s) 2097
ErhI CCWWGG 2 cut(s) 39, 1290
FalI AAGNNNNNCTT 4 cut(s) 738, 770, 1472, 1504
FaqI GGGAC 3 cut(s) 980, 1118, 1214
FauI CCCGC 1 cut(s) 1191
FauNDI CATATG 1 cut(s) 2497
FbaI TGATCA 1 cut(s) 1878
FblI GTMKAC 2 cut(s) 907, 1897
Fnu4HI GCNGC 4 cut(s) 372, 1362, 1551, 1783
Fsp4HI GCNGC 4 cut(s) 372, 1362, 1551, 1783
FspBI CTAG 3 cut(s) 435, 2052, 2549
GlaI GCGC 1 cut(s) 15
GluI GCNGC 4 cut(s) 372, 1362, 1551, 1783
GsuI CTGGAG 1 cut(s) 1104
HaeIII GGCC 7 cut(s) 311, 433, 595, 694, 1403, 2336, 2571
HapII CCGG 4 cut(s) 6, 796, 806, 1692
HgaI GACGC 1 cut(s) 5
HhaI GCGC 1 cut(s) 16
Hin6I GCGC 1 cut(s) 14
HinP1I GCGC 1 cut(s) 14
HindIII AAGCTT 2 cut(s) 232, 980
HinfI GANTC 7 cut(s) 341, 488, 616, 727, 1229, 2122, 2359
HpaII CCGG 4 cut(s) 6, 796, 806, 1692
HphI GGTGA 4 cut(s) 466, 656, 793, 1601
Hpy166II GTNNAC 4 cut(s) 46, 803, 908, 1898
Hpy188I TCNGA 3 cut(s) 493, 1151, 2352
Hpy8I GTNNAC 4 cut(s) 46, 803, 908, 1898
Hpy99I CGWCG 1 cut(s) 21
HpyAV CCTTC 6 cut(s) 39, 658, 1446, 1522, 1622, 2326
HpyCH4III ACNGT 7 cut(s) 172, 337, 387, 934, 1024, 1561, 2179
HpyCH4IV ACGT 1 cut(s) 512
HpyF10VI GCNNNNNNNGC 5 cut(s) 22, 1400, 1664, 1788, 2323
HpyF3I CTNAG 7 cut(s) 96, 396, 755, 1331, 2304, 2328, 2457
HpySE526I ACGT 1 cut(s) 512
HspAI GCGC 1 cut(s) 14
Ksp22I TGATCA 1 cut(s) 1878
Kzo9I GATC 7 cut(s) 831, 1146, 1273, 1597, 1878, 2224, 2431
Lsp1109I GCAGC 3 cut(s) 383, 1373, 1537
LweI GCATC 4 cut(s) 856, 1276, 1801, 2313
MaeI CTAG 3 cut(s) 435, 2052, 2549
MaeII ACGT 1 cut(s) 512
MaeIII GTNAC 7 cut(s) 166, 919, 950, 1073, 1969, 2179, 2281
MalI GATC 7 cut(s) 833, 1148, 1275, 1599, 1880, 2226, 2433
MboI GATC 7 cut(s) 831, 1146, 1273, 1597, 1878, 2224, 2431
MboII GAAGA 9 cut(s) 1106, 1187, 1224, 1397, 1644, 1715, 2261, 2441, 2461
MflI RGATCY 1 cut(s) 2431
MhlI GDGCHC 2 cut(s) 1606, 1752
MlyI GAGTC 3 cut(s) 350, 497, 2368
MmeI TCCRAC 4 cut(s) 471, 742, 1260, 1803
Mph1103I ATGCAT 1 cut(s) 2097
MroXI GAANNNNTTC 1 cut(s) 1940
MslI CAYNNNNRTG 5 cut(s) 207, 874, 1025, 1447, 2195
MspA1I CMGCKG 2 cut(s) 608, 863
MspI CCGG 4 cut(s) 6, 796, 806, 1692
MspR9I CCNGG 2 cut(s) 7, 2474
MvaI CCWGG 1 cut(s) 2474
MvnI CGCG 1 cut(s) 16
MwoI GCNNNNNNNGC 5 cut(s) 22, 1400, 1664, 1788, 2323
NciI CCSGG 1 cut(s) 7
NcoI CCATGG 1 cut(s) 1290
NdeI CATATG 1 cut(s) 2497
NdeII GATC 7 cut(s) 831, 1146, 1273, 1597, 1878, 2224, 2431
NlaIV GGNNCC 3 cut(s) 693, 996, 2064
NmeAIII GCCGAG 1 cut(s) 621
NmuCI GTSAC 3 cut(s) 166, 1073, 1969
NsiI ATGCAT 1 cut(s) 2097
NspI RCATGY 3 cut(s) 1087, 1713, 2095
OliI CACNNNNGTG 1 cut(s) 874
PaeI GCATGC 1 cut(s) 2095
PaqCI CACCTGC 1 cut(s) 769
PceI AGGCCT 1 cut(s) 1403
PdmI GAANNNNTTC 1 cut(s) 1940
PfeI GAWTC 4 cut(s) 616, 727, 1229, 2122
PfoI TCCNGGA 1 cut(s) 2472
PkrI GCNGC 4 cut(s) 373, 1363, 1552, 1784
PleI GAGTC 3 cut(s) 349, 496, 2367
PpsI GAGTC 3 cut(s) 349, 496, 2367
PpuMI RGGWCCY 1 cut(s) 994
PshBI ATTAAT 1 cut(s) 1670
PsiI TTATAA 2 cut(s) 191, 822
Psp5II RGGWCCY 1 cut(s) 994
Psp6I CCWGG 1 cut(s) 2472
PspGI CCWGG 1 cut(s) 2472
PspN4I GGNNCC 3 cut(s) 693, 996, 2064
PspPI GGNCC 5 cut(s) 431, 692, 994, 2335, 2570
PspPPI RGGWCCY 1 cut(s) 994
PsuI RGATCY 1 cut(s) 2431
PvuII CAGCTG 1 cut(s) 863
RsaI GTAC 8 cut(s) 45, 1031, 1487, 1563, 1577, 2048, 2110, 2489
RsaNI GTAC 8 cut(s) 44, 1030, 1486, 1562, 1576, 2047, 2109, 2488
RseI CAYNNNNRTG 5 cut(s) 207, 874, 1025, 1447, 2195
SatI GCNGC 4 cut(s) 372, 1362, 1551, 1783
Sau3AI GATC 7 cut(s) 831, 1146, 1273, 1597, 1878, 2224, 2431
Sau96I GGNCC 5 cut(s) 431, 692, 994, 2335, 2570
SchI GAGTC 3 cut(s) 350, 497, 2368
ScrFI CCNGG 2 cut(s) 7, 2474
SduI GDGCHC 2 cut(s) 1606, 1752
SfaNI GCATC 4 cut(s) 856, 1276, 1801, 2313
SfcI CTRYAG 1 cut(s) 1894
SinI GGWCC 1 cut(s) 994
SmiMI CAYNNNNRTG 5 cut(s) 207, 874, 1025, 1447, 2195
SmlI CTYRAG 2 cut(s) 214, 872
SmoI CTYRAG 2 cut(s) 214, 872
SphI GCATGC 1 cut(s) 2095
SseBI AGGCCT 1 cut(s) 1403
SsiI CCGC 4 cut(s) 608, 1198, 1782, 2311
SspI AATATT 2 cut(s) 1141, 2400
SspMI CTAG 3 cut(s) 435, 2052, 2549
StuI AGGCCT 1 cut(s) 1403
StyD4I CCNGG 2 cut(s) 5, 2472
StyI CCWWGG 2 cut(s) 39, 1290
TaaI ACNGT 7 cut(s) 172, 337, 387, 934, 1024, 1561, 2179
TaiI ACGT 1 cut(s) 515
TaqI TCGA 3 cut(s) 549, 742, 2125
TaqII GACCGA 1 cut(s) 925
TatI WGTACW 2 cut(s) 1029, 2487
TauI GCSGC 1 cut(s) 1785
TfiI GAWTC 4 cut(s) 616, 727, 1229, 2122
TscAI CASTG 4 cut(s) 175, 340, 939, 1799
TseFI GTSAC 3 cut(s) 166, 1073, 1969
TseI GCWGC 3 cut(s) 371, 1361, 1550
Tsp45I GTSAC 3 cut(s) 166, 1073, 1969
TspGWI ACGGA 2 cut(s) 690, 2359
TspRI CASTG 4 cut(s) 175, 340, 939, 1799
VpaK11BI GGWCC 1 cut(s) 994
VspI ATTAAT 1 cut(s) 1670
XagI CCTNNNNNAGG 1 cut(s) 38
XapI RAATTY 1 cut(s) 379
XbaI TCTAGA 1 cut(s) 2548
XceI RCATGY 3 cut(s) 1087, 1713, 2095
XmiI GTMKAC 2 cut(s) 907, 1897
XmnI GAANNNNTTC 1 cut(s) 1940
XspI CTAG 3 cut(s) 435, 2052, 2549
Zsp2I ATGCAT 1 cut(s) 2097
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.