MD07G1092900.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004858
Physical Location & Seq
Forward (+)
9896957 .. 9898652
1696 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1092900.v1.1.491

Sequence Viewer

Length: 861 bp
ATGATTCTTGGGTTTTGTAGAAAAGGGTTGATTACGATGGGAGAGAGCTTGTTGCATGTGATGTGGAAATTTCAGTGTGATCCGGATGGTATTATGTATAACATTCTGATCAATGCAAACTGTGAGAGGGGGCAGGCAGACGATGCACTTCATTGGGTGCATTTGATGATTGCAAGGGCTTGTAAGCCGAGCACCGTTACATTTAGCACTGTCATAAATGCGTTGTGTAAGGAGGGGAATATGGTGGAAGCTAGGAAGATTTTTTATGGGATCCCAGATATGGGTATTTCTCCAAGTACTACTATATACAATACCATGATGGACGGGTACGTTAAGGCAAGGGACATTGGCCAGGCCAACATGATCTATGAAGAAATGAGAAACAAAGGCATATCTCCTGATGGCATAACTTTTAATATCTTGATTGCAGGGCATTACAAGTATGGAAGGGAAGAGGATGGAGACCGTTTGTTAAAGGATATATCTGTGTCAGGGTTGCTTCCAGATCCTTCATTGTATGACATCATGGTTTCTGGGTTATGTTGGGCAGGTCGGTTGGACGATGCCATTGAATTTTTAGAGGATACGCTAGGAAAAGGATTAGCTCTGAGTGTGGTCGCTTTCAACTCAATTATTGCAGCTTGTAGCAGAGTAGGGTTAGAGCAGAAGGCCCTTAAGGCCTATAAGTTTATGATTACATTTGGTATAACTCCTTCATCTTCTACATGTAGTTCTTTGCTTATGGGTTTATCCAAGAAGGGGAACCTGCAAGAAGCCAGAGAGTTTTTGTGTAAGATGATAGAGAAGGGGTTCCCTATCAAAAAAGCTGTTTTCACGGTGCTTTTGGATGGGTACTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 map00500
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

287

Amino Acids

31.64

Weight (kDa)

6.44

Isoelectric Point (pI)

34.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 28 - 77 1.8e-12 PPR repeat family
PPR_long PF17177 44 - 153 3.6e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 61 - 88 8.3e-09 PPR repeat
PPR_2 PF13041 63 - 99 5.8e-06 PPR repeat family
PPR_3 PF13812 92 - 142 3.2e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 98 - 146 2.4e-15 PPR repeat family
PPR PF01535 102 - 131 1.1e-06 PPR repeat
PPR_1 PF12854 165 - 194 1.9e-07 PPR repeat
PPR_2 PF13041 204 - 252 3.4e-07 PPR repeat family
PPR_2 PF13041 238 - 285 4.1e-06 PPR repeat family
PPR PF01535 242 - 271 3.4e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 539, 774
AccIII TCCGGA 1 cut(s) 82
AclWI GGATC 4 cut(s) 74, 265, 278, 500
AcoI YGGCCR 1 cut(s) 349
AcsI RAATTY 2 cut(s) 68, 572
AfaI GTAC 3 cut(s) 298, 329, 854
AfiI CCNNNNNNNGG 3 cut(s) 280, 281, 759
AflII CTTAAG 1 cut(s) 674
AflIII ACRYGT 1 cut(s) 725
AgsI TTSAA 2 cut(s) 572, 625
AjnI CCWGG 1 cut(s) 351
AluBI AGCT 5 cut(s) 48, 251, 605, 641, 827
AluI AGCT 5 cut(s) 48, 251, 605, 641, 827
Alw21I GWGCWC 1 cut(s) 194
Alw26I GTCTC 1 cut(s) 456
AlwI GGATC 4 cut(s) 74, 265, 278, 500
Aor13HI TCCGGA 1 cut(s) 82
AoxI GGCC 4 cut(s) 349, 354, 669, 678
ApeKI GCWGC 1 cut(s) 638
ApoI RAATTY 2 cut(s) 68, 572
Asp700I GAANNNNTTC 1 cut(s) 809
AspS9I GGNCC 1 cut(s) 670
BalI TGGCCA 1 cut(s) 351
BamHI GGATCC 1 cut(s) 270
BarI GAAGNNNNNNTAC 2 cut(s) 697, 729
Bbv12I GWGCWC 1 cut(s) 194
BbvI GCAGC 1 cut(s) 650
BccI CCATC 6 cut(s) 31, 80, 313, 395, 452, 842
BciT130I CCWGG 1 cut(s) 353
BciVI GTATCC 1 cut(s) 577
BclI TGATCA 1 cut(s) 108
BcoDI GTCTC 1 cut(s) 456
BfaI CTAG 2 cut(s) 252, 590
BfrI CTTAAG 1 cut(s) 674
BfuAI ACCTGC 2 cut(s) 539, 774
BfuI GTATCC 1 cut(s) 577
BglI GCCNNNNNGGC 1 cut(s) 677
BisI GCNGC 1 cut(s) 639
BlsI GCNGC 1 cut(s) 640
BmcAI AGTACT 1 cut(s) 298
Bme1390I CCNGG 1 cut(s) 353
BmgT120I GGNCC 1 cut(s) 670
BmiI GGNNCC 3 cut(s) 272, 764, 812
BmrFI CCNGG 1 cut(s) 353
BmsI GCATC 2 cut(s) 133, 553
BsaI GGTCTC 1 cut(s) 456
BsaWI WCCGGW 1 cut(s) 82
Bsc4I CCNNNNNNNGG 3 cut(s) 280, 281, 759
BseAI TCCGGA 1 cut(s) 82
BseBI CCWGG 1 cut(s) 353
BseGI GGATG 3 cut(s) 91, 463, 853
BseLI CCNNNNNNNGG 3 cut(s) 280, 281, 759
BseMII CTCAG 1 cut(s) 599
BseXI GCAGC 1 cut(s) 650
BshFI GGCC 4 cut(s) 351, 356, 671, 680
BsiHKAI GWGCWC 1 cut(s) 194
BsiSI CCGG 1 cut(s) 83
BslFI GGGAC 1 cut(s) 356
BslI CCNNNNNNNGG 3 cut(s) 280, 281, 759
BsmAI GTCTC 1 cut(s) 456
BsmFI GGGAC 1 cut(s) 356
BsnI GGCC 4 cut(s) 351, 356, 671, 680
Bso31I GGTCTC 1 cut(s) 456
Bsp1286I GDGCHC 1 cut(s) 194
Bsp13I TCCGGA 1 cut(s) 82
Bsp143I GATC 5 cut(s) 79, 108, 270, 363, 505
BspANI GGCC 4 cut(s) 351, 356, 671, 680
BspCNI CTCAG 1 cut(s) 600
BspEI TCCGGA 1 cut(s) 82
BspLI GGNNCC 3 cut(s) 272, 764, 812
BspMI ACCTGC 2 cut(s) 539, 774
BspPI GGATC 4 cut(s) 74, 265, 278, 500
BspTI CTTAAG 1 cut(s) 674
BspTNI GGTCTC 1 cut(s) 456
BssMI GATC 5 cut(s) 79, 108, 270, 363, 505
Bst2UI CCWGG 1 cut(s) 353
Bst4CI ACNGT 5 cut(s) 122, 196, 211, 467, 838
Bst6I CTCTTC 1 cut(s) 447
BstAFI CTTAAG 1 cut(s) 674
BstAPI GCANNNNNTGC 1 cut(s) 143
BstC8I GCNNGC 1 cut(s) 135
BstDEI CTNAG 1 cut(s) 608
BstF5I GGATG 3 cut(s) 91, 463, 853
BstKTI GATC 5 cut(s) 82, 111, 273, 366, 508
BstMAI GTCTC 1 cut(s) 456
BstMBI GATC 5 cut(s) 79, 108, 270, 363, 505
BstMWI GCNNNNNNNGC 2 cut(s) 143, 677
BstNI CCWGG 1 cut(s) 353
BstNSI RCATGY 2 cut(s) 59, 729
BstSCI CCNGG 1 cut(s) 351
BstV1I GCAGC 1 cut(s) 650
BstX2I RGATCY 2 cut(s) 270, 505
BstYI RGATCY 2 cut(s) 270, 505
BsuI GTATCC 1 cut(s) 577
BsuRI GGCC 4 cut(s) 351, 356, 671, 680
BtsCI GGATG 3 cut(s) 91, 463, 853
BtsIMutI CAGTG 2 cut(s) 80, 207
BveI ACCTGC 2 cut(s) 539, 774
Cac8I GCNNGC 1 cut(s) 135
Cfr13I GGNCC 1 cut(s) 670
Csp6I GTAC 3 cut(s) 297, 328, 853
CviAII CATG 5 cut(s) 56, 316, 361, 526, 726
CviQI GTAC 3 cut(s) 297, 328, 853
DdeI CTNAG 1 cut(s) 608
DpnI GATC 5 cut(s) 81, 110, 272, 365, 507
DpnII GATC 5 cut(s) 79, 108, 270, 363, 505
EaeI YGGCCR 1 cut(s) 349
Eam1104I CTCTTC 1 cut(s) 447
EarI CTCTTC 1 cut(s) 447
Eco147I AGGCCT 1 cut(s) 680
Eco31I GGTCTC 1 cut(s) 456
EcoO109I RGGNCCY 1 cut(s) 670
EcoRII CCWGG 1 cut(s) 351
FaeI CATG 5 cut(s) 59, 319, 364, 529, 729
FaqI GGGAC 1 cut(s) 356
FatI CATG 5 cut(s) 55, 315, 360, 525, 725
FbaI TGATCA 1 cut(s) 108
Fnu4HI GCNGC 1 cut(s) 639
FokI GGATG 2 cut(s) 98, 470
Fsp4HI GCNGC 1 cut(s) 639
FspBI CTAG 2 cut(s) 252, 590
GluI GCNGC 1 cut(s) 639
HaeIII GGCC 4 cut(s) 351, 356, 671, 680
HapII CCGG 1 cut(s) 83
Hin1II CATG 5 cut(s) 59, 319, 364, 529, 729
HinfI GANTC 1 cut(s) 4
HpaII CCGG 1 cut(s) 83
Hpy188I TCNGA 2 cut(s) 108, 609
Hpy188III TCNNGA 4 cut(s) 83, 398, 421, 503
HpyAV CCTTC 6 cut(s) 441, 519, 661, 723, 751, 799
HpyCH4III ACNGT 5 cut(s) 122, 196, 211, 467, 838
HpyCH4IV ACGT 1 cut(s) 330
HpyCH4V TGCA 8 cut(s) 55, 116, 146, 160, 173, 428, 638, 769
HpyF10VI GCNNNNNNNGC 2 cut(s) 143, 677
HpyF3I CTNAG 1 cut(s) 608
HpySE526I ACGT 1 cut(s) 330
Hsp92II CATG 5 cut(s) 59, 319, 364, 529, 729
Kpn2I TCCGGA 1 cut(s) 82
Ksp22I TGATCA 1 cut(s) 108
Kzo9I GATC 5 cut(s) 79, 108, 270, 363, 505
Lsp1109I GCAGC 1 cut(s) 650
LweI GCATC 2 cut(s) 133, 553
MaeI CTAG 2 cut(s) 252, 590
MaeII ACGT 1 cut(s) 330
MaeIII GTNAC 1 cut(s) 196
MalI GATC 5 cut(s) 81, 110, 272, 365, 507
MboI GATC 5 cut(s) 79, 108, 270, 363, 505
MboII GAAGA 4 cut(s) 268, 383, 464, 711
MflI RGATCY 2 cut(s) 270, 505
MhlI GDGCHC 1 cut(s) 194
MlsI TGGCCA 1 cut(s) 351
MluCI AATT 3 cut(s) 68, 572, 630
MluNI TGGCCA 1 cut(s) 351
MmeI TCCRAC 1 cut(s) 537
MnlI CCTC 4 cut(s) 120, 226, 448, 574
Mox20I TGGCCA 1 cut(s) 351
MroI TCCGGA 1 cut(s) 82
MroXI GAANNNNTTC 1 cut(s) 809
MscI TGGCCA 1 cut(s) 351
MseI TTAA 4 cut(s) 333, 414, 473, 675
Msp20I TGGCCA 1 cut(s) 351
MspCI CTTAAG 1 cut(s) 674
MspI CCGG 1 cut(s) 83
MspR9I CCNGG 1 cut(s) 353
MvaI CCWGG 1 cut(s) 353
MwoI GCNNNNNNNGC 2 cut(s) 143, 677
NdeII GATC 5 cut(s) 79, 108, 270, 363, 505
NlaIII CATG 5 cut(s) 59, 319, 364, 529, 729
NlaIV GGNNCC 3 cut(s) 272, 764, 812
NmeAIII GCCGAG 1 cut(s) 213
NspI RCATGY 2 cut(s) 59, 729
PceI AGGCCT 1 cut(s) 680
PciI ACATGT 1 cut(s) 725
PdmI GAANNNNTTC 1 cut(s) 809
PfeI GAWTC 1 cut(s) 4
PkrI GCNGC 1 cut(s) 640
PscI ACATGT 1 cut(s) 725
Psp6I CCWGG 1 cut(s) 351
PspGI CCWGG 1 cut(s) 351
PspN4I GGNNCC 3 cut(s) 272, 764, 812
PspPI GGNCC 1 cut(s) 670
PsuI RGATCY 2 cut(s) 270, 505
RsaI GTAC 3 cut(s) 298, 329, 854
RsaNI GTAC 3 cut(s) 297, 328, 853
SaqAI TTAA 4 cut(s) 333, 414, 473, 675
SatI GCNGC 1 cut(s) 639
Sau3AI GATC 5 cut(s) 79, 108, 270, 363, 505
Sau96I GGNCC 1 cut(s) 670
ScaI AGTACT 1 cut(s) 298
ScrFI CCNGG 1 cut(s) 353
SduI GDGCHC 1 cut(s) 194
SetI ASST 8 cut(s) 50, 253, 333, 553, 607, 643, 768, 829
SfaNI GCATC 2 cut(s) 133, 553
SfiI GGCCNNNNNGGCC 1 cut(s) 677
SmlI CTYRAG 1 cut(s) 674
SmoI CTYRAG 1 cut(s) 674
Sse9I AATT 3 cut(s) 68, 572, 630
SseBI AGGCCT 1 cut(s) 680
SspMI CTAG 2 cut(s) 252, 590
StuI AGGCCT 1 cut(s) 680
StyD4I CCNGG 1 cut(s) 351
TaaI ACNGT 5 cut(s) 122, 196, 211, 467, 838
TaiI ACGT 1 cut(s) 333
TasI AATT 3 cut(s) 68, 572, 630
TatI WGTACW 1 cut(s) 296
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 4 cut(s) 333, 414, 473, 675
Tru9I TTAA 4 cut(s) 333, 414, 473, 675
TscAI CASTG 2 cut(s) 80, 214
TseI GCWGC 1 cut(s) 638
TspDTI ATGAA 4 cut(s) 140, 384, 501, 705
TspRI CASTG 2 cut(s) 80, 214
Vha464I CTTAAG 1 cut(s) 674
XapI RAATTY 2 cut(s) 68, 572
XceI RCATGY 2 cut(s) 59, 729
XmnI GAANNNNTTC 1 cut(s) 809
XspI CTAG 2 cut(s) 252, 590
ZrmI AGTACT 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.