Rroxscaffold_3G00244320

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0004858
Physical Location & Seq
Reverse (-)
36995227 .. 37000714
5488 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00244320.1

Sequence Viewer

Length: 2673 bp
ATGGTGGTGGTGAGTAACTCGGTTGGAATTGAAACTGAGGAGGAGGTGGGTAGCTTGGCTGCAATGGAAATTGGGAAGGAGGTGAATTCAAAAGTTGCTTCTCCTGCGGCGTTGAGCTTCAAATGTCCTGTGAGGCGCGTCGCATTCGCCCTTCTCTCAAGAGAAACTGCATTTGGGTTCTTTAAACTAGCCTTTAGGGATGATAATCAGCAGACTGTGGAGTCATGTTGTGTGGCTGCACATGTTTTGGCGGCCGAAAATCTACGGTTTCTGGCTCAAGATGTGGTTTCGTGTGTGATTGGGAGGATTGGGCCGAGTAGGAGTAGGGATTTGGTGGGGTTTATGTGGGGAGGGCATTGCGAATACGAGTCGGATTTTTCAGTTCTTGATACACTAATGAGGGGGTTCTTGAATGCGGAAATGGGCGCCGAGGCTTTGGAGGTTTTGAGGAGGATGAGGGAGGTGGGAGTGAGGCCGAGTTTGTCAGCAGTTGCGATTCTGTTCAGGTTGTTGATAAGGGTTGGTGATTATGGTAGTGTGTGGAAGGTGTTTAGGGATATGATTAGAGAAGGGCCTTATCCATGTAATTATGTTTTTAGTGCAATGATTCTTGGGTTTTGTCGAAAGGGGTTGGTTAGAGTTGGGGAGGGTTTGTTACAGGTGATGTGGAAACTCCAATGTGAACCGGATGTTTTTGCTTATAACATTGTGATCTATGCAAATTGTGTGAGAGGGCAGACAGCTGATGCGCTTGAATGGGTGGAGTTGATGATTGCGAGGGGATGTAAACCGAGCAATGTTACATTTAATACAGTCATAAGTGCATTGTGTAAGGAAGGAAATGTGGTGGAGGCAAGGAAGCTTTTTGATGATGTCCCAGACACGGGTGTTTATCCGAATACCATAATGTACAATACCATGATGGATGGGTATGTTAAGGCAAGGGAGATTGGTCAGGCTAACATGCTTTTTGAAGAAATGAGAAACAAGAGCATTTCTCCAGATGGCATAACTTTCAATATCTTGATTGGAGGGCATTACAAGTATGGAAGCGAAGATGATGGAGACAGGTTGTTGCGGGATTTATCTGTGTCAGGATTACTTCCAGATTCTTCATTGTATGATATCTATGTTTCTGTGTTGTGTTGGGCAGATCGGTTGGACGATGCCATGGCAGTTTTAGAGGATATGCTTGAAAGAGGATTGCCTCTAAGTGTGGTCGCTTTCAACTCTGTTATTGCAGCTTGTAGCAGAGTAGGGTTGGAAGAAAAAGCATATAAGGCCTTTAACTTCATGATTTCATTTGGTATAACTCCTTCATCTTCTACATGTAGTTCTTTGCTTATGGGTTTATCTAAGAAGGGTTACTTGCAAGAAGCCAGAGAGCTTTTGTGTAAAATGATGGAGAAGGGGTTTCCTATCAGAAAAGCTGCTTTTACGGTGCTTTTGGATGGGTACTTTAGGATGGCATTTATCAATGGACTATCTAAAGCCGGTTTAATGGAAGAAGCATATGATATGTTTCAAGCAATGTCAAGAAGAGGGTTTGTGCTCAATAATTTTGTCTATAATTCTTTGATTGGTGGGTTTTGCTACTGTGGTAAGTTGAGTGATGCACTGAGGTTGGAGAGAGAGATGAGGCAAAGGGGTCTTATTCCGGATATTTTTACCACCAATATGATCATTCATGGGTTCTGTAGACAGGGGAGGATGAAGTCAGCAATTAATACATTTATGAACATGTTCCAAACTGGGTTAACCCCAGATATCGTCACTTACAATACATTAATTGGTGGTTATTGTAAAGCATTTGACATGGTTAGGGCAGATGAATTTTTGTATAAAATGTACGCTAGTGGATGGGAACCAGATATCACAACCTATAATATACGGATGCATGGTTTTTGTAGTAGCCGAAAGATAAATCGAGCTGTGATGATTCTGGATGAGCTTGTATCAAGAGGTGTTGTTCCAGACACAGAAACATACAACACTATGATGAATGGTGTTTGTATTGACATTCTGGATCGTGCTATGATTTTAACCGCTAAGTTGCTTAAGTTGGCGTTCCTTCCAAATGTTGTTACAGCTAATGTATTGTTGTCTCAGTTCCGCAGGCAGGGGATGCCTGAGAAGGCCCTAATGTGGGGTCAGAAGTTGAGTCAGTTTTCCTTTTGTTTTGATGAAATTACCTACAAAATATTGGACAGAGCCTATCATAATATGCAAGAAGATCCTGCAATTCCAAGTGCAACACCTGAGAAAAGCCTCTTCTTGGATTTCCTCATGTACATTACGTATGATTATTTATGTAAAAATAAACCTTGCAGAGATCACTCTTCCATTGATGTCTGGCATGTGATAAGTGCATCTGTGAAAGCAGCATGCTCCCCTGCAAAAGATTCTGAGGAAGAAGTGAAAGATCTGCGATTTGTATCTACTTGTTCCCTTGACCAAGCTGAAATATCAATCGACGAACTCTACCATACTGCAGTACTGTGGGTGGAGCCCTTTCTCCTGAGATGCAGCCTCCAAAGGGCCACAGGAGTTGATATCCGTAGTCTCTTCACCACAACATGCACATTGACATATCTTGATCATGCACAAATGCAGCAAAACAATGAGAAAGATTCTCGGGATATGGAAGTTTGTGGATGGGATGATGATTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 map00500
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

890

Amino Acids

100.16

Weight (kDa)

5.43

Isoelectric Point (pI)

36.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 194 - 242 4.1e-06 PPR repeat family
PPR_3 PF13812 219 - 276 1e-05 Pentatricopeptide repeat domain
PPR_2 PF13041 229 - 278 3.2e-12 PPR repeat family
PPR_1 PF12854 260 - 291 1.6e-11 PPR repeat
PPR_2 PF13041 264 - 301 2.4e-08 PPR repeat family
PPR PF01535 267 - 292 3.2e-06 PPR repeat
PPR_3 PF13812 287 - 344 1.6e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 299 - 347 3e-15 PPR repeat family
PPR PF01535 302 - 331 8e-06 PPR repeat
PPR_2 PF13041 405 - 453 9.9e-08 PPR repeat family
PPR_2 PF13041 439 - 487 5.4e-06 PPR repeat family
PPR_2 PF13041 490 - 531 3.6e-09 PPR repeat family
PPR_3 PF13812 508 - 562 3.8e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 514 - 547 9.6e-08 PPR repeat
PPR_2 PF13041 519 - 567 3.5e-13 PPR repeat family
PPR PF01535 522 - 551 7.6e-06 PPR repeat
PPR_3 PF13812 542 - 600 1.4e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 549 - 578 7.6e-07 PPR repeat
PPR_2 PF13041 553 - 602 3.2e-16 PPR repeat family
PPR_1 PF12854 585 - 616 8.8e-08 PPR repeat
PPR_2 PF13041 588 - 636 8.5e-14 PPR repeat family
PPR_2 PF13041 623 - 671 1.7e-13 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 702
AasI GACNNNNNNGTC 1 cut(s) 220
AccB1I GGYRCC 1 cut(s) 425
AccI GTMKAC 1 cut(s) 1699
AccII CGCG 1 cut(s) 138
AccIII TCCGGA 1 cut(s) 1657
AciI CCGC 6 cut(s) 107, 251, 416, 1078, 2046, 2113
AclWI GGATC 2 cut(s) 2034, 2228
AcoI YGGCCR 1 cut(s) 252
AcsI RAATTY 2 cut(s) 85, 1832
AcyI GRCGYC 1 cut(s) 426
AfaI GTAC 5 cut(s) 911, 1457, 1850, 2291, 2496
AfiI CCNNNNNNNGG 7 cut(s) 883, 884, 2119, 2145, 2146, 2275, 2536
AflII CTTAAG 1 cut(s) 2057
AflIII ACRYGT 3 cut(s) 241, 1328, 1740
AjuI GAANNNNNNNTTGG 2 cut(s) 156, 188
Alw21I GWGCWC 1 cut(s) 1554
Alw26I GTCTC 3 cut(s) 1059, 2109, 2567
AlwI GGATC 2 cut(s) 2034, 2228
Ama87I CYCGRG 1 cut(s) 2634
Aor13HI TCCGGA 1 cut(s) 1657
AoxI GGCC 7 cut(s) 252, 311, 473, 572, 1281, 2136, 2538
ApeKI GCWGC 7 cut(s) 59, 236, 1241, 1430, 2381, 2526, 2611
ApoI RAATTY 2 cut(s) 85, 1832
AseI ATTAAT 2 cut(s) 1725, 1787
Asp700I GAANNNNTTC 1 cut(s) 1742
AspLEI GCGC 3 cut(s) 138, 428, 751
AspS9I GGNCC 4 cut(s) 311, 572, 2137, 2538
AsuHPI GGTGA 5 cut(s) 22, 94, 536, 673, 2560
AvaI CYCGRG 1 cut(s) 2634
BanI GGYRCC 1 cut(s) 425
BanII GRGCYC 1 cut(s) 2511
BarI GAAGNNNNNNTAC 2 cut(s) 1300, 1332
Bbv12I GWGCWC 1 cut(s) 1554
BbvI GCAGC 7 cut(s) 46, 223, 1253, 1417, 2393, 2538, 2623
BccI CCATC 9 cut(s) 916, 920, 998, 1055, 1396, 1445, 1459, 1854, 2649
BclI TGATCA 2 cut(s) 1680, 2596
BcoDI GTCTC 3 cut(s) 1059, 2109, 2567
BfaI CTAG 3 cut(s) 188, 1854, 2671
BfmI CTRYAG 2 cut(s) 1696, 2490
BfoI RGCGCY 1 cut(s) 429
BfrI CTTAAG 1 cut(s) 2057
BglII AGATCT 1 cut(s) 2422
BisI GCNGC 9 cut(s) 60, 108, 237, 252, 1242, 1431, 2382, 2527, 2612
BlsI GCNGC 9 cut(s) 61, 109, 238, 253, 1243, 1432, 2383, 2528, 2613
BmcAI AGTACT 1 cut(s) 2496
BmeT110I CYCGRG 1 cut(s) 2634
BmgT120I GGNCC 4 cut(s) 311, 572, 2137, 2538
BmiI GGNNCC 3 cut(s) 427, 1866, 2508
BmrI ACTGGG 1 cut(s) 1761
BmsI GCATC 7 cut(s) 736, 1156, 1603, 1884, 2115, 2378, 2513
BmuI ACTGGG 1 cut(s) 1761
BplI GAGNNNNNCTC 2 cut(s) 982, 1014
BpmI CTGGAG 1 cut(s) 984
BpuEI CTTGAG 2 cut(s) 142, 261
BsaAI YACGTR 1 cut(s) 2298
BsaBI GATNNNNATC 2 cut(s) 204, 2434
BsaHI GRCGYC 1 cut(s) 426
BsaJI CCNNGG 2 cut(s) 429, 1170
BsaWI WCCGGW 2 cut(s) 685, 1657
BsaXI ACNNNNNCTCC 4 cut(s) 638, 668, 1397, 1427
Bsc4I CCNNNNNNNGG 7 cut(s) 883, 884, 2119, 2145, 2146, 2275, 2536
Bse118I RCCGGY 1 cut(s) 1493
Bse1I ACTGG 1 cut(s) 1756
Bse3DI GCAATG 5 cut(s) 69, 355, 609, 802, 1536
Bse8I GATNNNNATC 2 cut(s) 204, 2434
BseAI TCCGGA 1 cut(s) 1657
BseDI CCNNGG 2 cut(s) 429, 1170
BseJI GATNNNNATC 2 cut(s) 204, 2434
BseLI CCNNNNNNNGG 7 cut(s) 883, 884, 2119, 2145, 2146, 2275, 2536
BseMI GCAATG 5 cut(s) 69, 355, 609, 802, 1536
BseMII CTCAG 7 cut(s) 27, 1610, 2120, 2121, 2250, 2397, 2510
BseNI ACTGG 1 cut(s) 1756
BseRI GAGGAG 3 cut(s) 53, 56, 463
BseX3I CGGCCG 1 cut(s) 252
BseXI GCAGC 7 cut(s) 46, 223, 1253, 1417, 2393, 2538, 2623
BsgI GTGCAG 1 cut(s) 222
Bsh1236I CGCG 1 cut(s) 138
Bsh1285I CGRYCG 1 cut(s) 255
BshFI GGCC 7 cut(s) 254, 313, 475, 574, 1283, 2138, 2540
BshNI GGYRCC 1 cut(s) 425
BsiEI CGRYCG 1 cut(s) 255
BsiHKAI GWGCWC 1 cut(s) 1554
BsiHKCI CYCGRG 1 cut(s) 2634
BsiSI CCGG 3 cut(s) 686, 1494, 1658
BslFI GGGAC 1 cut(s) 860
BslI CCNNNNNNNGG 7 cut(s) 883, 884, 2119, 2145, 2146, 2275, 2536
BsmAI GTCTC 3 cut(s) 1059, 2109, 2567
BsmFI GGGAC 1 cut(s) 860
BsmI GAATGC 2 cut(s) 143, 418
BsnI GGCC 7 cut(s) 254, 313, 475, 574, 1283, 2138, 2540
BsoBI CYCGRG 1 cut(s) 2634
Bsp1286I GDGCHC 2 cut(s) 1554, 2511
Bsp13I TCCGGA 1 cut(s) 1657
Bsp1407I TGTACA 2 cut(s) 909, 2289
Bsp143I GATC 8 cut(s) 711, 1153, 1680, 2026, 2233, 2332, 2422, 2596
Bsp19I CCATGG 1 cut(s) 1170
BspACI CCGC 6 cut(s) 107, 251, 416, 1078, 2046, 2113
BspANI GGCC 7 cut(s) 254, 313, 475, 574, 1283, 2138, 2540
BspCNI CTCAG 7 cut(s) 28, 1611, 2119, 2122, 2251, 2398, 2511
BspEI TCCGGA 1 cut(s) 1657
BspFNI CGCG 1 cut(s) 138
BspHI TCATGA 1 cut(s) 1293
BspLI GGNNCC 3 cut(s) 427, 1866, 2508
BspMAI CTGCAG 1 cut(s) 2494
BspPI GGATC 2 cut(s) 2034, 2228
BspT107I GGYRCC 1 cut(s) 425
BspTI CTTAAG 1 cut(s) 2057
BsrDI GCAATG 5 cut(s) 69, 355, 609, 802, 1536
BsrFI RCCGGY 1 cut(s) 1493
BsrGI TGTACA 2 cut(s) 909, 2289
BsrI ACTGG 1 cut(s) 1756
BssAI RCCGGY 1 cut(s) 1493
BssECI CCNNGG 2 cut(s) 429, 1170
BssMI GATC 8 cut(s) 711, 1153, 1680, 2026, 2233, 2332, 2422, 2596
BssNI GRCGYC 1 cut(s) 426
BssT1I CCWWGG 1 cut(s) 1170
Bst4CI ACNGT 6 cut(s) 217, 267, 814, 1441, 1598, 2499
Bst6I CTCTTC 4 cut(s) 1534, 2276, 2344, 2570
BstACI GRCGYC 1 cut(s) 426
BstAFI CTTAAG 1 cut(s) 2057
BstAPI GCANNNNNTGC 1 cut(s) 2125
BstAUI TGTACA 2 cut(s) 909, 2289
BstBAI YACGTR 1 cut(s) 2298
BstC8I GCNNGC 2 cut(s) 2117, 2386
BstDSI CCRYGG 1 cut(s) 1170
BstFNI CGCG 1 cut(s) 138
BstH2I RGCGCY 1 cut(s) 429
BstHHI GCGC 3 cut(s) 138, 428, 751
BstKTI GATC 8 cut(s) 714, 1156, 1683, 2029, 2236, 2335, 2425, 2599
BstMAI GTCTC 3 cut(s) 1059, 2109, 2567
BstMBI GATC 8 cut(s) 711, 1153, 1680, 2026, 2233, 2332, 2422, 2596
BstMCI CGRYCG 1 cut(s) 255
BstMWI GCNNNNNNNGC 3 cut(s) 104, 1280, 2125
BstNSI RCATGY 7 cut(s) 245, 967, 1332, 1744, 2360, 2388, 2580
BstSFI CTRYAG 2 cut(s) 1696, 2490
BstSNI TACGTA 1 cut(s) 2298
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 7 cut(s) 46, 223, 1253, 1417, 2393, 2538, 2623
BstX2I RGATCY 2 cut(s) 2233, 2422
BstYI RGATCY 2 cut(s) 2233, 2422
BstZI CGGCCG 1 cut(s) 252
BsuRI GGCC 7 cut(s) 254, 313, 475, 574, 1283, 2138, 2540
BtgI CCRYGG 1 cut(s) 1170
BtsIMutI CAGTG 1 cut(s) 1616
Cac8I GCNNGC 2 cut(s) 2117, 2386
CciI TCATGA 1 cut(s) 1293
CfoI GCGC 3 cut(s) 138, 428, 751
Cfr10I RCCGGY 1 cut(s) 1493
Cfr13I GGNCC 4 cut(s) 311, 572, 2137, 2538
CseI GACGC 1 cut(s) 127
Csp6I GTAC 5 cut(s) 910, 1456, 1849, 2290, 2495
CspCI CAANNNNNGTGG 2 cut(s) 2530, 2565
CviQI GTAC 5 cut(s) 910, 1456, 1849, 2290, 2495
DinI GGCGCC 1 cut(s) 427
DpnI GATC 8 cut(s) 713, 1155, 1682, 2028, 2235, 2334, 2424, 2598
DpnII GATC 8 cut(s) 711, 1153, 1680, 2026, 2233, 2332, 2422, 2596
DraI TTTAAA 1 cut(s) 184
DrdI GACNNNNNNGTC 1 cut(s) 220
DseDI GACNNNNNNGTC 1 cut(s) 220
EaeI YGGCCR 1 cut(s) 252
EagI CGGCCG 1 cut(s) 252
Eam1104I CTCTTC 4 cut(s) 1534, 2276, 2344, 2570
EarI CTCTTC 4 cut(s) 1534, 2276, 2344, 2570
EclXI CGGCCG 1 cut(s) 252
Eco105I TACGTA 1 cut(s) 2298
Eco130I CCWWGG 1 cut(s) 1170
Eco147I AGGCCT 1 cut(s) 1283
Eco24I GRGCYC 1 cut(s) 2511
Eco32I GATATC 4 cut(s) 1126, 1768, 1873, 2554
Eco52I CGGCCG 1 cut(s) 252
Eco88I CYCGRG 1 cut(s) 2634
EcoO109I RGGNCCY 2 cut(s) 572, 2137
EcoRI GAATTC 1 cut(s) 85
EcoRV GATATC 4 cut(s) 1126, 1768, 1873, 2554
EcoT14I CCWWGG 1 cut(s) 1170
EcoT22I ATGCAT 1 cut(s) 1899
EcoT38I GRGCYC 1 cut(s) 2511
EgeI GGCGCC 1 cut(s) 427
EheI GGCGCC 1 cut(s) 427
ErhI CCWWGG 1 cut(s) 1170
FalI AAGNNNNNCTT 2 cut(s) 1352, 1384
FaqI GGGAC 1 cut(s) 860
FauI CCCGC 1 cut(s) 1071
FauNDI CATATG 1 cut(s) 1513
FbaI TGATCA 2 cut(s) 1680, 2596
FblI GTMKAC 1 cut(s) 1699
Fnu4HI GCNGC 9 cut(s) 60, 108, 237, 252, 1242, 1431, 2382, 2527, 2612
FriOI GRGCYC 1 cut(s) 2511
Fsp4HI GCNGC 9 cut(s) 60, 108, 237, 252, 1242, 1431, 2382, 2527, 2612
FspBI CTAG 3 cut(s) 188, 1854, 2671
GlaI GCGC 3 cut(s) 137, 427, 750
GluI GCNGC 9 cut(s) 60, 108, 237, 252, 1242, 1431, 2382, 2527, 2612
GsuI CTGGAG 1 cut(s) 984
HaeII RGCGCY 1 cut(s) 429
HaeIII GGCC 7 cut(s) 254, 313, 475, 574, 1283, 2138, 2540
HapII CCGG 3 cut(s) 686, 1494, 1658
HgaI GACGC 1 cut(s) 127
HhaI GCGC 3 cut(s) 138, 428, 751
Hin1I GRCGYC 1 cut(s) 426
Hin6I GCGC 3 cut(s) 136, 426, 749
HinP1I GCGC 3 cut(s) 136, 426, 749
HincII GTYRAC 1 cut(s) 1758
HindII GTYRAC 1 cut(s) 1758
HindIII AAGCTT 1 cut(s) 860
HinfI GANTC 9 cut(s) 221, 368, 496, 607, 1109, 1939, 2161, 2402, 2630
HpaI GTTAAC 1 cut(s) 1758
HpaII CCGG 3 cut(s) 686, 1494, 1658
HphI GGTGA 5 cut(s) 22, 94, 536, 673, 2560
Hpy166II GTNNAC 4 cut(s) 683, 788, 1700, 1758
Hpy188I TCNGA 5 cut(s) 373, 897, 1424, 2154, 2407
Hpy8I GTNNAC 4 cut(s) 683, 788, 1700, 1758
Hpy99I CGWCG 2 cut(s) 143, 2477
HpyCH4III ACNGT 6 cut(s) 217, 267, 814, 1441, 1598, 2499
HpyCH4IV ACGT 1 cut(s) 2297
HpyF10VI GCNNNNNNNGC 3 cut(s) 104, 1280, 2125
HpySE526I ACGT 1 cut(s) 2297
Hsp92I GRCGYC 1 cut(s) 426
HspAI GCGC 3 cut(s) 136, 426, 749
KasI GGCGCC 1 cut(s) 425
Kpn2I TCCGGA 1 cut(s) 1657
Ksp22I TGATCA 2 cut(s) 1680, 2596
KspAI GTTAAC 1 cut(s) 1758
Kzo9I GATC 8 cut(s) 711, 1153, 1680, 2026, 2233, 2332, 2422, 2596
LmnI GCTCC 2 cut(s) 2393, 2506
Lsp1109I GCAGC 7 cut(s) 46, 223, 1253, 1417, 2393, 2538, 2623
LweI GCATC 7 cut(s) 736, 1156, 1603, 1884, 2115, 2378, 2513
MaeI CTAG 3 cut(s) 188, 1854, 2671
MaeII ACGT 1 cut(s) 2297
MaeIII GTNAC 6 cut(s) 14, 654, 799, 1364, 1771, 2083
MalI GATC 8 cut(s) 713, 1155, 1682, 2028, 2235, 2334, 2424, 2598
MboI GATC 8 cut(s) 711, 1153, 1680, 2026, 2233, 2332, 2422, 2596
MflI RGATCY 2 cut(s) 2233, 2422
MhlI GDGCHC 2 cut(s) 1554, 2511
Mly113I GGCGCC 1 cut(s) 426
MlyI GAGTC 3 cut(s) 230, 377, 2170
MmeI TCCRAC 5 cut(s) 4, 351, 1140, 1242, 1605
Mph1103I ATGCAT 1 cut(s) 1899
MroI TCCGGA 1 cut(s) 1657
MroXI GAANNNNTTC 1 cut(s) 1742
MslI CAYNNNNRTG 2 cut(s) 1676, 1997
MspA1I CMGCKG 1 cut(s) 743
MspCI CTTAAG 1 cut(s) 2057
MspI CCGG 3 cut(s) 686, 1494, 1658
Mva1269I GAATGC 2 cut(s) 143, 418
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 3 cut(s) 104, 1280, 2125
NarI GGCGCC 1 cut(s) 426
NcoI CCATGG 1 cut(s) 1170
NdeI CATATG 1 cut(s) 1513
NdeII GATC 8 cut(s) 711, 1153, 1680, 2026, 2233, 2332, 2422, 2596
NlaIV GGNNCC 3 cut(s) 427, 1866, 2508
NmeAIII GCCGAG 3 cut(s) 339, 454, 501
NmuCI GTSAC 1 cut(s) 1771
NsiI ATGCAT 1 cut(s) 1899
NspI RCATGY 7 cut(s) 245, 967, 1332, 1744, 2360, 2388, 2580
PaeI GCATGC 1 cut(s) 2388
PagI TCATGA 1 cut(s) 1293
PceI AGGCCT 1 cut(s) 1283
PciI ACATGT 3 cut(s) 241, 1328, 1740
PctI GAATGC 2 cut(s) 143, 418
PdmI GAANNNNTTC 1 cut(s) 1742
PfeI GAWTC 6 cut(s) 496, 607, 1109, 1939, 2402, 2630
PkrI GCNGC 9 cut(s) 61, 109, 238, 253, 1243, 1432, 2383, 2528, 2613
PleI GAGTC 3 cut(s) 229, 376, 2169
PluTI GGCGCC 1 cut(s) 429
PpsI GAGTC 3 cut(s) 229, 376, 2169
Ppu21I YACGTR 1 cut(s) 2298
PscI ACATGT 3 cut(s) 241, 1328, 1740
PshBI ATTAAT 2 cut(s) 1725, 1787
PsiI TTATAA 1 cut(s) 702
PspN4I GGNNCC 3 cut(s) 427, 1866, 2508
PspPI GGNCC 4 cut(s) 311, 572, 2137, 2538
PstI CTGCAG 1 cut(s) 2494
PsuI RGATCY 2 cut(s) 2233, 2422
PvuII CAGCTG 1 cut(s) 743
RsaI GTAC 5 cut(s) 911, 1457, 1850, 2291, 2496
RsaNI GTAC 5 cut(s) 910, 1456, 1849, 2290, 2495
RseI CAYNNNNRTG 2 cut(s) 1676, 1997
SatI GCNGC 9 cut(s) 60, 108, 237, 252, 1242, 1431, 2382, 2527, 2612
Sau3AI GATC 8 cut(s) 711, 1153, 1680, 2026, 2233, 2332, 2422, 2596
Sau96I GGNCC 4 cut(s) 311, 572, 2137, 2538
ScaI AGTACT 1 cut(s) 2496
SchI GAGTC 3 cut(s) 230, 377, 2170
SduI GDGCHC 2 cut(s) 1554, 2511
SfaNI GCATC 7 cut(s) 736, 1156, 1603, 1884, 2115, 2378, 2513
SfcI CTRYAG 2 cut(s) 1696, 2490
SfoI GGCGCC 1 cut(s) 427
SmiMI CAYNNNNRTG 2 cut(s) 1676, 1997
SmlI CTYRAG 3 cut(s) 157, 276, 2057
SmoI CTYRAG 3 cut(s) 157, 276, 2057
SnaBI TACGTA 1 cut(s) 2298
SphI GCATGC 1 cut(s) 2388
SseBI AGGCCT 1 cut(s) 1283
SsiI CCGC 6 cut(s) 107, 251, 416, 1078, 2046, 2113
SspDI GGCGCC 1 cut(s) 425
SspI AATATT 1 cut(s) 2202
SspMI CTAG 3 cut(s) 188, 1854, 2671
StuI AGGCCT 1 cut(s) 1283
StyI CCWWGG 1 cut(s) 1170
TaaI ACNGT 6 cut(s) 217, 267, 814, 1441, 1598, 2499
TaiI ACGT 1 cut(s) 2300
TaqI TCGA 3 cut(s) 622, 1927, 2472
TatI WGTACW 3 cut(s) 909, 2289, 2494
TauI GCSGC 2 cut(s) 110, 254
TfiI GAWTC 6 cut(s) 496, 607, 1109, 1939, 2402, 2630
TscAI CASTG 1 cut(s) 1623
TseFI GTSAC 1 cut(s) 1771
TseI GCWGC 7 cut(s) 59, 236, 1241, 1430, 2381, 2526, 2611
Tsp45I GTSAC 1 cut(s) 1771
TspGWI ACGGA 2 cut(s) 1906, 2546
TspRI CASTG 1 cut(s) 1623
Vha464I CTTAAG 1 cut(s) 2057
VspI ATTAAT 2 cut(s) 1725, 1787
XapI RAATTY 2 cut(s) 85, 1832
XceI RCATGY 7 cut(s) 245, 967, 1332, 1744, 2360, 2388, 2580
XmiI GTMKAC 1 cut(s) 1699
XmnI GAANNNNTTC 1 cut(s) 1742
XspI CTAG 3 cut(s) 188, 1854, 2671
ZrmI AGTACT 1 cut(s) 2496
Zsp2I ATGCAT 1 cut(s) 1899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.