MD07G1093100.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004858
Physical Location & Seq
Forward (+)
9899164 .. 9900924
1761 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1093100.v1.1.491

Sequence Viewer

Length: 384 bp
ATGATGAATGGTGCTTGTGTTGACATATTGGACCGTGCTATGATTTTAATGGCTAAATTGCTCAAGTTGGCGTTTCTTCCAAATACTGTTACAATTAATGTGTTATTGTCCCAGTTCTGCAAGCAGGGGAAGCCTGAGAAGGCCCTCATGTGGGGTCAGAAGTTGAGTGAGTTTTCCACTTGCTTTGACAAAATTACGTATAAATTATTGGACAGAGCTTATCATAATCTGCAAGAAGATTCTGAAATTTCCAGTGGAACAGCTGAAAACAGCCTCTTCCTGGATTTCCTCATGTACATTACATATGATTATTTATGTAGAAATAAACCTTGCAGGGATGCAAGTCAAAATCCTCTTAAGTTAATTGATTTTAAAGGCTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 map00500
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

128

Amino Acids

14.52

Weight (kDa)

7.58

Isoelectric Point (pI)

28.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 27 - 70 3.6e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 246
AfaI GTAC 1 cut(s) 296
AfiI CCNNNNNNNGG 3 cut(s) 150, 151, 280
AflII CTTAAG 1 cut(s) 356
AjnI CCWGG 1 cut(s) 279
AluBI AGCT 2 cut(s) 218, 263
AluI AGCT 2 cut(s) 218, 263
AoxI GGCC 1 cut(s) 141
ApoI RAATTY 1 cut(s) 246
AseI ATTAAT 1 cut(s) 96
AspS9I GGNCC 2 cut(s) 31, 142
AvaII GGWCC 1 cut(s) 31
BciT130I CCWGG 1 cut(s) 281
BfrI CTTAAG 1 cut(s) 356
Bme1390I CCNGG 1 cut(s) 281
Bme18I GGWCC 1 cut(s) 31
BmgT120I GGNCC 2 cut(s) 31, 142
BmiI GGNNCC 1 cut(s) 379
BmrFI CCNGG 1 cut(s) 281
BmrI ACTGGG 1 cut(s) 106
BmsI GCATC 1 cut(s) 328
BmuI ACTGGG 1 cut(s) 106
BpuEI CTTGAG 1 cut(s) 47
BsaAI YACGTR 1 cut(s) 198
Bsc4I CCNNNNNNNGG 3 cut(s) 150, 151, 280
Bse1I ACTGG 2 cut(s) 112, 252
BseBI CCWGG 1 cut(s) 281
BseGI GGATG 1 cut(s) 343
BseLI CCNNNNNNNGG 3 cut(s) 150, 151, 280
BseMII CTCAG 1 cut(s) 126
BseNI ACTGG 2 cut(s) 112, 252
BshFI GGCC 1 cut(s) 143
BslFI GGGAC 1 cut(s) 94
BslI CCNNNNNNNGG 3 cut(s) 150, 151, 280
BsmFI GGGAC 1 cut(s) 94
BsnI GGCC 1 cut(s) 143
Bsp1407I TGTACA 1 cut(s) 294
BspANI GGCC 1 cut(s) 143
BspCNI CTCAG 1 cut(s) 127
BspLI GGNNCC 1 cut(s) 379
BspTI CTTAAG 1 cut(s) 356
BsrGI TGTACA 1 cut(s) 294
BsrI ACTGG 2 cut(s) 112, 252
Bst2UI CCWGG 1 cut(s) 281
Bst4CI ACNGT 2 cut(s) 35, 88
Bst6I CTCTTC 1 cut(s) 281
BstAFI CTTAAG 1 cut(s) 356
BstAUI TGTACA 1 cut(s) 294
BstBAI YACGTR 1 cut(s) 198
BstC8I GCNNGC 1 cut(s) 122
BstDEI CTNAG 1 cut(s) 135
BstF5I GGATG 1 cut(s) 343
BstMWI GCNNNNNNNGC 1 cut(s) 130
BstNI CCWGG 1 cut(s) 281
BstSCI CCNGG 1 cut(s) 279
BstSNI TACGTA 1 cut(s) 198
BsuRI GGCC 1 cut(s) 143
BtsCI GGATG 1 cut(s) 343
BtsIMutI CAGTG 1 cut(s) 259
Cac8I GCNNGC 1 cut(s) 122
Cfr13I GGNCC 2 cut(s) 31, 142
Csp6I GTAC 1 cut(s) 295
CspCI CAANNNNNGTGG 2 cut(s) 166, 201
CviAII CATG 2 cut(s) 148, 292
CviJI RGCY 7 cut(s) 53, 133, 143, 218, 263, 273, 378
CviKI_1 RGCY 7 cut(s) 53, 133, 143, 218, 263, 273, 378
CviQI GTAC 1 cut(s) 295
DdeI CTNAG 1 cut(s) 135
DraI TTTAAA 1 cut(s) 373
Eam1104I CTCTTC 1 cut(s) 281
EarI CTCTTC 1 cut(s) 281
Eco105I TACGTA 1 cut(s) 198
Eco47I GGWCC 1 cut(s) 31
EcoO109I RGGNCCY 1 cut(s) 142
EcoRII CCWGG 1 cut(s) 279
FaeI CATG 2 cut(s) 151, 295
FaiI YATR 9 cut(s) 26, 41, 149, 201, 225, 293, 304, 306, 316
FaqI GGGAC 1 cut(s) 94
FatI CATG 2 cut(s) 147, 291
FauNDI CATATG 1 cut(s) 304
FokI GGATG 1 cut(s) 350
HaeIII GGCC 1 cut(s) 143
Hin1II CATG 2 cut(s) 151, 295
HincII GTYRAC 1 cut(s) 22
HindII GTYRAC 1 cut(s) 22
HinfI GANTC 1 cut(s) 239
Hpy166II GTNNAC 1 cut(s) 22
Hpy188I TCNGA 2 cut(s) 159, 244
Hpy188III TCNNGA 1 cut(s) 381
Hpy8I GTNNAC 1 cut(s) 22
HpyAV CCTTC 1 cut(s) 133
HpyCH4III ACNGT 2 cut(s) 35, 88
HpyCH4IV ACGT 1 cut(s) 197
HpyCH4V TGCA 4 cut(s) 120, 232, 333, 341
HpyF10VI GCNNNNNNNGC 1 cut(s) 130
HpyF3I CTNAG 1 cut(s) 135
HpySE526I ACGT 1 cut(s) 197
Hsp92II CATG 2 cut(s) 151, 295
LmnI GCTCC 1 cut(s) 383
LpnPI CCDG 7 cut(s) 110, 125, 147, 265, 266, 293, 319
LweI GCATC 1 cut(s) 328
MaeII ACGT 1 cut(s) 197
MaeIII GTNAC 1 cut(s) 88
MboII GAAGA 3 cut(s) 68, 248, 268
MluCI AATT 6 cut(s) 56, 93, 192, 203, 246, 363
MnlI CCTC 4 cut(s) 155, 284, 299, 363
MseI TTAA 5 cut(s) 47, 96, 357, 362, 372
MspA1I CMGCKG 1 cut(s) 263
MspCI CTTAAG 1 cut(s) 356
MspR9I CCNGG 1 cut(s) 281
MvaI CCWGG 1 cut(s) 281
MwoI GCNNNNNNNGC 1 cut(s) 130
NdeI CATATG 1 cut(s) 304
NlaIII CATG 2 cut(s) 151, 295
NlaIV GGNNCC 1 cut(s) 379
PfeI GAWTC 1 cut(s) 239
PfoI TCCNGGA 1 cut(s) 279
Ppu21I YACGTR 1 cut(s) 198
PshBI ATTAAT 1 cut(s) 96
Psp6I CCWGG 1 cut(s) 279
PspGI CCWGG 1 cut(s) 279
PspN4I GGNNCC 1 cut(s) 379
PspPI GGNCC 2 cut(s) 31, 142
PvuII CAGCTG 1 cut(s) 263
RsaI GTAC 1 cut(s) 296
RsaNI GTAC 1 cut(s) 295
SaqAI TTAA 5 cut(s) 47, 96, 357, 362, 372
Sau96I GGNCC 2 cut(s) 31, 142
ScrFI CCNGG 1 cut(s) 281
SetI ASST 4 cut(s) 200, 220, 265, 331
SfaNI GCATC 1 cut(s) 328
SinI GGWCC 1 cut(s) 31
SmlI CTYRAG 2 cut(s) 62, 356
SmoI CTYRAG 2 cut(s) 62, 356
SnaBI TACGTA 1 cut(s) 198
Sse9I AATT 6 cut(s) 56, 93, 192, 203, 246, 363
StyD4I CCNGG 1 cut(s) 279
TaaI ACNGT 2 cut(s) 35, 88
TaiI ACGT 1 cut(s) 200
TasI AATT 6 cut(s) 56, 93, 192, 203, 246, 363
TatI WGTACW 1 cut(s) 294
TfiI GAWTC 1 cut(s) 239
Tru1I TTAA 5 cut(s) 47, 96, 357, 362, 372
Tru9I TTAA 5 cut(s) 47, 96, 357, 362, 372
TscAI CASTG 1 cut(s) 259
TspDTI ATGAA 1 cut(s) 20
TspRI CASTG 1 cut(s) 259
Vha464I CTTAAG 1 cut(s) 356
VpaK11BI GGWCC 1 cut(s) 31
VspI ATTAAT 1 cut(s) 96
XapI RAATTY 1 cut(s) 246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.