FvH4_5g29330

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008224
Physical Location & Seq
Forward (+)
20326472 .. 20327833
1362 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g29330.t1

Sequence Viewer

Length: 1362 bp
ATGGAGACTTTGCTGCTTAGACCATTCTTATCTGTCACTACAGAATCATTTCCGAACTCCTTTACCTATTTGATAGCTGGAACCACTTTGATCATTGCGGTTCTCCTTTTAGAACTCAGATCAACGAGGATATACCTAGTTGATTTCACTTGCTATCTGCCTCCTGGTAGATTGCGGGTTCCAAAGGCGCACTTCATCGAACATGTGGAGATGTCTAACATCAATGGCAGAGAGGGTATTGATTTCCAAGTAAAGGTATGGGAAAGATCAGGCATTGGTTCAGAGACTAGCATGCCTGATCCACTGCACGAAATCCCGCCTCGTGAGTCTTTGGATGATACTCGTAACGAAATTGAAATGGTTTTCTTCACTGTTGTTGAAGACCTTCTTTCCAAGCACAAAATTGATCCAAAAAGCATCGACATTCTTGTGTCAAATTGCAGCATTTTCTGCTCAACACCATCCATTAGTTCAATGATCATAAACAAGTTTGGATTTCGAAGCGACATAAAGAGCGTCAACCTCAGTGGAATGGGCTGCAGTGCTGGAATCTTGTCAATTTGTTTGGCCAAGGATCTTCTCAAAGTTCACAAAAACTCGTTGGCATTAGTCCTCAGCATGGAAGCTGTAACTCCCAACGGCTATGCGGGTAAGGAAAAATCAATGCTTCTTCCAAACGTCTTGTTTCGGATGGGAGGGGCTGCTATTCTACTGTCGAATAGGAAGCAGGACAAAGGCATGTCAAAGTACAAGCTCCAGCATCTTGTCCGAACCCACCTTGGTTCGAATGACGAAGCATACGAATCTGTTTTCCATAAAGCAGATGAGGATGGATGTGAAGGGGTCTCACTATCAAGAGCCCTGTTGAGTGTGGCAACAAAAGCTCTTAAAACAAACATATCAGCTCTGGGGCCACTTGTGCTACCATACTCCGAGCAGTTTCGGTTTGCATGGTCAGTGATCCGTAAGAAAATCAGGATCTTACCAAGGCAGAAGGGAGTTTATGTGCCAAATTTCAAGAAGGCTATCGAACACTACTGTATACATGCTGGTGGAAGAGCAGTGATAGATGGCATAGAAAAGAGTCTGAAACTGCAAAAGGAAGATGCAGAGGCTTCAAGAATGACATTACATAGATTTGGAAACACATCTTCTTCTTCAATTTGGTATGAGCTTTGTTATTTGGAAGCAAAAGGAATGGTGAAGAAAGGTAACAAAGTATGGCAAATTGCATTTGGAAGTGGCTTCAAGTGCAACAGTGCAGTTTGGAAATGCGTTTCGGATGTTGATCCAACAAAAAGAAATGCATGGTCAGACGGCATCAATTTGTACCCTATAATTGATGATGAAATCCCAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

454

Amino Acids

50.52

Weight (kDa)

8.84

Isoelectric Point (pI)

35.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 39 - 324 4.9e-119 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 341 - 424 6.4e-12 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 346 - 424 2e-10 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1042
AciI CCGC 4 cut(s) 98, 175, 317, 647
AclWI GGATC 6 cut(s) 293, 401, 582, 955, 986, 1283
AcoI YGGCCR 1 cut(s) 567
AcsI RAATTY 1 cut(s) 1012
AfaI GTAC 2 cut(s) 749, 1331
AfiI CCNNNNNNNGG 2 cut(s) 253, 619
AflIII ACRYGT 1 cut(s) 202
AgsI TTSAA 7 cut(s) 356, 380, 474, 1018, 1119, 1161, 1249
AjnI CCWGG 1 cut(s) 163
AluBI AGCT 6 cut(s) 77, 626, 754, 884, 905, 1174
AluI AGCT 6 cut(s) 77, 626, 754, 884, 905, 1174
Alw26I GTCTC 2 cut(s) 278, 850
AlwI GGATC 6 cut(s) 293, 401, 582, 955, 986, 1283
AoxI GGCC 2 cut(s) 567, 911
ApeKI GCWGC 4 cut(s) 13, 441, 537, 701
ApoI RAATTY 1 cut(s) 1012
Asp700I GAANNNNTTC 2 cut(s) 48, 384
AspLEI GCGC 1 cut(s) 190
AspS9I GGNCC 1 cut(s) 911
AsuHPI GGTGA 1 cut(s) 1213
AsuII TTCGAA 2 cut(s) 499, 785
BalI TGGCCA 1 cut(s) 569
BanII GRGCYC 1 cut(s) 862
BauI CACGAG 1 cut(s) 321
BbsI GAAGAC 1 cut(s) 387
BbvCI CCTCAGC 1 cut(s) 614
BbvI GCAGC 3 cut(s) 453, 524, 688
BccI CCATC 4 cut(s) 469, 685, 824, 1064
BceAI ACGGC 2 cut(s) 655, 1333
BciT130I CCWGG 1 cut(s) 165
BclI TGATCA 2 cut(s) 90, 477
BcoDI GTCTC 2 cut(s) 278, 850
BfaI CTAG 2 cut(s) 137, 288
BfmI CTRYAG 2 cut(s) 39, 538
BisI GCNGC 4 cut(s) 14, 442, 538, 702
BlsI GCNGC 4 cut(s) 15, 443, 539, 703
Bme1390I CCNGG 1 cut(s) 165
BmgT120I GGNCC 1 cut(s) 911
BmiI GGNNCC 3 cut(s) 82, 180, 912
BmrFI CCNGG 1 cut(s) 165
BmsI GCATC 4 cut(s) 426, 769, 1096, 1329
BpiI GAAGAC 1 cut(s) 387
BpmI CTGGAG 1 cut(s) 740
Bpu10I CCTNAGC 1 cut(s) 614
Bpu14I TTCGAA 2 cut(s) 499, 785
BsaBI GATNNNNATC 1 cut(s) 1287
BsaI GGTCTC 1 cut(s) 850
BsaJI CCNNGG 3 cut(s) 570, 778, 986
BsaXI ACNNNNNCTCC 2 cut(s) 990, 1020
Bsc4I CCNNNNNNNGG 2 cut(s) 253, 619
Bse3DI GCAATG 1 cut(s) 93
Bse8I GATNNNNATC 1 cut(s) 1287
BseBI CCWGG 1 cut(s) 165
BseDI CCNNGG 3 cut(s) 570, 778, 986
BseGI GGATG 6 cut(s) 340, 461, 696, 835, 839, 1288
BseJI GATNNNNATC 1 cut(s) 1287
BseLI CCNNNNNNNGG 2 cut(s) 253, 619
BseMI GCAATG 1 cut(s) 93
BseMII CTCAG 3 cut(s) 130, 538, 628
BseXI GCAGC 3 cut(s) 453, 524, 688
BsgI GTGCAG 2 cut(s) 290, 1281
BshFI GGCC 2 cut(s) 569, 913
BslI CCNNNNNNNGG 2 cut(s) 253, 619
BsmAI GTCTC 2 cut(s) 278, 850
BsnI GGCC 2 cut(s) 569, 913
Bso31I GGTCTC 1 cut(s) 850
Bsp119I TTCGAA 2 cut(s) 499, 785
Bsp1286I GDGCHC 1 cut(s) 862
BspACI CCGC 4 cut(s) 98, 175, 317, 647
BspANI GGCC 2 cut(s) 569, 913
BspCNI CTCAG 3 cut(s) 129, 537, 627
BspLI GGNNCC 3 cut(s) 82, 180, 912
BspMAI CTGCAG 1 cut(s) 542
BspPI GGATC 6 cut(s) 293, 401, 582, 955, 986, 1283
BspQI GCTCTTC 1 cut(s) 1051
BspT104I TTCGAA 2 cut(s) 499, 785
BspTNI GGTCTC 1 cut(s) 850
BsrDI GCAATG 1 cut(s) 93
BssECI CCNNGG 3 cut(s) 570, 778, 986
BssNAI GTATAC 1 cut(s) 1043
BssSI CACGAG 1 cut(s) 321
BssT1I CCWWGG 3 cut(s) 570, 778, 986
Bst1107I GTATAC 1 cut(s) 1043
Bst2BI CACGAG 1 cut(s) 321
Bst2UI CCWGG 1 cut(s) 165
Bst4CI ACNGT 4 cut(s) 373, 714, 1040, 1259
Bst6I CTCTTC 1 cut(s) 1051
BstAPI GCANNNNNTGC 1 cut(s) 450
BstBI TTCGAA 2 cut(s) 499, 785
BstC8I GCNNGC 1 cut(s) 293
BstDEI CTNAG 4 cut(s) 17, 116, 524, 614
BstF5I GGATG 6 cut(s) 340, 461, 696, 835, 839, 1288
BstHHI GCGC 1 cut(s) 190
BstMAI GTCTC 2 cut(s) 278, 850
BstMWI GCNNNNNNNGC 4 cut(s) 450, 881, 919, 1251
BstNI CCWGG 1 cut(s) 165
BstNSI RCATGY 4 cut(s) 206, 295, 742, 1049
BstSCI CCNGG 1 cut(s) 163
BstSFI CTRYAG 2 cut(s) 39, 538
BstV1I GCAGC 3 cut(s) 453, 524, 688
BstV2I GAAGAC 1 cut(s) 387
BstX2I RGATCY 2 cut(s) 574, 978
BstYI RGATCY 2 cut(s) 574, 978
BstZ17I GTATAC 1 cut(s) 1043
BsuRI GGCC 2 cut(s) 569, 913
BtsCI GGATG 6 cut(s) 340, 461, 696, 835, 839, 1288
BtsI GCAGTG 3 cut(s) 302, 547, 1068
BtsIMutI CAGTG 7 cut(s) 302, 369, 532, 547, 963, 1068, 1264
Cac8I GCNNGC 1 cut(s) 293
CfoI GCGC 1 cut(s) 190
Cfr13I GGNCC 1 cut(s) 911
CseI GACGC 1 cut(s) 505
Csp6I GTAC 2 cut(s) 748, 1330
CspCI CAANNNNNGTGG 2 cut(s) 508, 543
CviAII CATG 7 cut(s) 203, 292, 619, 739, 951, 1046, 1308
CviQI GTAC 2 cut(s) 748, 1330
DdeI CTNAG 4 cut(s) 17, 116, 524, 614
EaeI YGGCCR 1 cut(s) 567
Eam1104I CTCTTC 1 cut(s) 1051
EarI CTCTTC 1 cut(s) 1051
Eco130I CCWWGG 3 cut(s) 570, 778, 986
Eco24I GRGCYC 1 cut(s) 862
Eco31I GGTCTC 1 cut(s) 850
EcoRII CCWGG 1 cut(s) 163
EcoT14I CCWWGG 3 cut(s) 570, 778, 986
EcoT22I ATGCAT 1 cut(s) 1309
EcoT38I GRGCYC 1 cut(s) 862
ErhI CCWWGG 3 cut(s) 570, 778, 986
FaeI CATG 7 cut(s) 206, 295, 622, 742, 954, 1049, 1311
FalI AAGNNNNNCTT 4 cut(s) 176, 208, 372, 404
FatI CATG 7 cut(s) 202, 291, 618, 738, 950, 1045, 1307
FauI CCCGC 3 cut(s) 168, 324, 640
FbaI TGATCA 2 cut(s) 90, 477
FblI GTMKAC 1 cut(s) 1042
Fnu4HI GCNGC 4 cut(s) 14, 442, 538, 702
FokI GGATG 6 cut(s) 347, 448, 703, 842, 846, 1295
FriOI GRGCYC 1 cut(s) 862
Fsp4HI GCNGC 4 cut(s) 14, 442, 538, 702
FspBI CTAG 2 cut(s) 137, 288
GlaI GCGC 1 cut(s) 189
GluI GCNGC 4 cut(s) 14, 442, 538, 702
GsuI CTGGAG 1 cut(s) 740
HaeIII GGCC 2 cut(s) 569, 913
HgaI GACGC 1 cut(s) 505
HhaI GCGC 1 cut(s) 190
Hin1II CATG 7 cut(s) 206, 295, 622, 742, 954, 1049, 1311
Hin6I GCGC 1 cut(s) 188
HinP1I GCGC 1 cut(s) 188
HincII GTYRAC 1 cut(s) 520
HindII GTYRAC 1 cut(s) 520
HinfI GANTC 5 cut(s) 44, 326, 549, 803, 1084
HphI GGTGA 1 cut(s) 1213
Hpy166II GTNNAC 3 cut(s) 520, 589, 1043
Hpy188I TCNGA 9 cut(s) 54, 119, 283, 690, 770, 934, 1089, 1282, 1315
Hpy188III TCNNGA 5 cut(s) 323, 855, 976, 1018, 1119
Hpy8I GTNNAC 3 cut(s) 520, 589, 1043
HpyAV CCTTC 4 cut(s) 395, 833, 988, 1015
HpyCH4III ACNGT 4 cut(s) 373, 714, 1040, 1259
HpyCH4IV ACGT 1 cut(s) 678
HpyF10VI GCNNNNNNNGC 4 cut(s) 450, 881, 919, 1251
HpyF3I CTNAG 4 cut(s) 17, 116, 524, 614
HpySE526I ACGT 1 cut(s) 678
Hsp92II CATG 7 cut(s) 206, 295, 622, 742, 954, 1049, 1311
HspAI GCGC 1 cut(s) 188
Ksp22I TGATCA 2 cut(s) 90, 477
LguI GCTCTTC 1 cut(s) 1051
LmnI GCTCC 1 cut(s) 759
Lsp1109I GCAGC 3 cut(s) 453, 524, 688
LweI GCATC 4 cut(s) 426, 769, 1096, 1329
MaeI CTAG 2 cut(s) 137, 288
MaeII ACGT 1 cut(s) 678
MaeIII GTNAC 4 cut(s) 34, 344, 628, 1211
MflI RGATCY 2 cut(s) 574, 978
MhlI GDGCHC 1 cut(s) 862
MlsI TGGCCA 1 cut(s) 569
MluCI AATT 9 cut(s) 351, 402, 436, 558, 1012, 1161, 1227, 1324, 1338
MluNI TGGCCA 1 cut(s) 569
MlyI GAGTC 2 cut(s) 335, 1093
MmeI TCCRAC 1 cut(s) 1316
MnlI CCTC 9 cut(s) 120, 171, 226, 330, 533, 623, 689, 820, 1105
Mox20I TGGCCA 1 cut(s) 569
Mph1103I ATGCAT 1 cut(s) 1309
MroXI GAANNNNTTC 2 cut(s) 48, 384
MscI TGGCCA 1 cut(s) 569
MseI TTAA 2 cut(s) 888, 1360
MslI CAYNNNNRTG 2 cut(s) 428, 1050
Msp20I TGGCCA 1 cut(s) 569
MspR9I CCNGG 1 cut(s) 165
MvaI CCWGG 1 cut(s) 165
MwoI GCNNNNNNNGC 4 cut(s) 450, 881, 919, 1251
NlaIII CATG 7 cut(s) 206, 295, 622, 742, 954, 1049, 1311
NlaIV GGNNCC 3 cut(s) 82, 180, 912
NmuCI GTSAC 1 cut(s) 34
NsiI ATGCAT 1 cut(s) 1309
NspI RCATGY 4 cut(s) 206, 295, 742, 1049
NspV TTCGAA 2 cut(s) 499, 785
PaeI GCATGC 1 cut(s) 295
PciI ACATGT 1 cut(s) 202
PciSI GCTCTTC 1 cut(s) 1051
PcsI WCGNNNNNNNCGW 1 cut(s) 798
PdmI GAANNNNTTC 2 cut(s) 48, 384
PfeI GAWTC 3 cut(s) 44, 549, 803
PkrI GCNGC 4 cut(s) 15, 443, 539, 703
PleI GAGTC 2 cut(s) 334, 1092
PpsI GAGTC 2 cut(s) 334, 1092
PscI ACATGT 1 cut(s) 202
Psp6I CCWGG 1 cut(s) 163
PspGI CCWGG 1 cut(s) 163
PspN4I GGNNCC 3 cut(s) 82, 180, 912
PspPI GGNCC 1 cut(s) 911
PstI CTGCAG 1 cut(s) 542
PsuI RGATCY 2 cut(s) 574, 978
RsaI GTAC 2 cut(s) 749, 1331
RsaNI GTAC 2 cut(s) 748, 1330
RseI CAYNNNNRTG 2 cut(s) 428, 1050
SapI GCTCTTC 1 cut(s) 1051
SaqAI TTAA 2 cut(s) 888, 1360
SatI GCNGC 4 cut(s) 14, 442, 538, 702
Sau96I GGNCC 1 cut(s) 911
SchI GAGTC 2 cut(s) 335, 1093
ScrFI CCNGG 1 cut(s) 165
SduI GDGCHC 1 cut(s) 862
SfaNI GCATC 4 cut(s) 426, 769, 1096, 1329
SfcI CTRYAG 2 cut(s) 39, 538
SfuI TTCGAA 2 cut(s) 499, 785
SmiMI CAYNNNNRTG 2 cut(s) 428, 1050
SphI GCATGC 1 cut(s) 295
Sse9I AATT 9 cut(s) 351, 402, 436, 558, 1012, 1161, 1227, 1324, 1338
SsiI CCGC 4 cut(s) 98, 175, 317, 647
SspMI CTAG 2 cut(s) 137, 288
StyD4I CCNGG 1 cut(s) 163
StyI CCWWGG 3 cut(s) 570, 778, 986
TaaI ACNGT 4 cut(s) 373, 714, 1040, 1259
TaiI ACGT 1 cut(s) 681
TaqI TCGA 6 cut(s) 198, 420, 499, 716, 785, 1029
TasI AATT 9 cut(s) 351, 402, 436, 558, 1012, 1161, 1227, 1324, 1338
TatI WGTACW 1 cut(s) 747
TfiI GAWTC 3 cut(s) 44, 549, 803
Tru1I TTAA 2 cut(s) 888, 1360
Tru9I TTAA 2 cut(s) 888, 1360
TscAI CASTG 7 cut(s) 309, 376, 532, 547, 963, 1068, 1264
TseFI GTSAC 1 cut(s) 34
TseI GCWGC 4 cut(s) 13, 441, 537, 701
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 2 cut(s) 184, 1362
TspGWI ACGGA 1 cut(s) 953
TspRI CASTG 7 cut(s) 309, 376, 532, 547, 963, 1068, 1264
XapI RAATTY 1 cut(s) 1012
XceI RCATGY 4 cut(s) 206, 295, 742, 1049
XmiI GTMKAC 1 cut(s) 1042
XmnI GAANNNNTTC 2 cut(s) 48, 384
XspI CTAG 2 cut(s) 137, 288
Zsp2I ATGCAT 1 cut(s) 1309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.