MD08G1230400.v1.1

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008224
Physical Location & Seq
Reverse (-)
29606348 .. 29607697
1350 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1230400.v1.1.491

Sequence Viewer

Length: 1350 bp
ATGGGGACTGCATTGCTTACATACATTACGGAATCTCCAATCTCCATCGCACATTTATTAGCCGCGACTGTCCTTATCATTGCGGTTCTTGGTTTCAAATTCAAATCCAAGAGTATATACCTTGTCGACTTCACTTGCTATCTGCCTCCCAATGGCTTGCGAATCGCAAAGGAGAGCTACATTGAACACCTTGAAATTTCAAACCTCTATGATGAAGAAAGCCGAAATTTCCAAATCAAGGTAATGAAAAGATCGGGAATCGGAGAGGAGAGTTGCTTGCCTATTACAACCCATGAGCTCCCTCCATATTCTTCTTTAAACGCTACTAGAAAAGAAACCGAAACAGTTCTCTTCTCTGTTGTCGAAGACCTTCTTTCCAAGCACAAAATAAATCCAACAAGCATTGACATCCTTGTCTCAAACTGTAGCCTTTTCTGCCCAACTCCGTCCGTTACATCAATGGTCATAAACAAGTTTGGATTCCGTAGCAACATAAAGAGCATCAGCCTCAGCGGAATGGGTTGCAGTGCTGGACTGTTGTCGATAAGTTTGGCTAAAGATCTTCTACGAGTCCACAAGAACTCATTGGCTTTAGTTCTCAGCATGGAAGCTGTAACTCCCAATGGTTATATAGGTAAAAGAAAGTCCATGCTTATTCCCAACCTTTTGTTTCGGATGGGAGGAGCTGCTATCCTATTGTCCAGTAGGAAGCAGGACAAGAAAATAGCAAAGTACGAGCTCCAGCATCTTGTCCGAACACACATTGGTTCGGATGATGAAGCATACCAATCTGTCTTCCAGCAACCAGATGAAGATGGCACTGTAGGGGTCTCACTGTCAAGGGCACTTGTACCTGTGGCCACCAAAGCTTTAAGAATTAACATATCCAAGTTGGGGCCACTCGTGCTGCCATATTCCGAGCAGCTACGATATGTATGGTCAGTAATCCGCAAGAAACTGTTTTTTTCACCGCGCCAGAAGAGAACTTATGTGCCGAATTTCAAGAAGGCTCTCCAGCATTTCTGTATTCATGCTGGCGGAAGGGCAGTCATTGATGGCATAGTAGATAGTCTGAAGCTGGACAAGGAAGATGGAGAAGCTTCAAGGATGACATTACATAGATTTGGCAATACTTCATCGTCTTCAGTTTGGTATGAGCTCTGCTATTTGGAGGCGAAAGGAAGGATGAAGAAAGGAAACCGAGTGTGGCAAATAGCCTTCGGAAGTGGCTTCAAGTGTAACAGTGCAATCTGGAAATGCATTTCGGATGTTGATCCAACCGTAAGAAATGCATGGTCCGACAGAATCTATTTGTATCCCGTCGATGAGATCTCAAATGAATCCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

450

Amino Acids

50.13

Weight (kDa)

9.29

Isoelectric Point (pI)

37.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 34 - 320 8.9e-120 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 337 - 419 1.5e-12 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 341 - 415 1.3e-08 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 413
AccI GTMKAC 1 cut(s) 126
AccII CGCG 2 cut(s) 65, 973
AciI CCGC 6 cut(s) 63, 83, 513, 949, 971, 1038
AclWI GGATC 1 cut(s) 1268
AcoI YGGCCR 1 cut(s) 858
AcsI RAATTY 4 cut(s) 98, 195, 226, 997
AcuI CTGAAG 2 cut(s) 1094, 1128
AfaI GTAC 2 cut(s) 734, 852
AfiI CCNNNNNNNGG 3 cut(s) 152, 238, 894
AgsI TTSAA 8 cut(s) 97, 103, 185, 194, 201, 1003, 1104, 1234
Alw21I GWGCWC 3 cut(s) 300, 741, 1161
Alw26I GTCTC 2 cut(s) 421, 835
AlwI GGATC 1 cut(s) 1268
AoxI GGCC 2 cut(s) 858, 896
ApeKI GCWGC 3 cut(s) 686, 907, 922
ApoI RAATTY 4 cut(s) 98, 195, 226, 997
Asp700I GAANNNNTTC 2 cut(s) 345, 369
AspLEI GCGC 1 cut(s) 975
AspS9I GGNCC 2 cut(s) 896, 1296
AsuHPI GGTGA 1 cut(s) 960
AvaII GGWCC 1 cut(s) 1296
BaeGI GKGCMC 1 cut(s) 847
BalI TGGCCA 1 cut(s) 860
BanII GRGCYC 3 cut(s) 300, 741, 1161
BauI CACGAG 1 cut(s) 902
BbsI GAAGAC 3 cut(s) 372, 787, 1134
Bbv12I GWGCWC 3 cut(s) 300, 741, 1161
BbvCI CCTCAGC 1 cut(s) 509
BbvI GCAGC 3 cut(s) 673, 894, 934
BccI CCATC 5 cut(s) 53, 670, 809, 1049, 1085
BciVI GTATCC 1 cut(s) 1326
BcoDI GTCTC 2 cut(s) 421, 835
BfaI CTAG 1 cut(s) 327
BfmI CTRYAG 2 cut(s) 424, 822
BfuI GTATCC 1 cut(s) 1326
BglII AGATCT 2 cut(s) 559, 1329
BisI GCNGC 4 cut(s) 63, 687, 908, 923
BlsI GCNGC 4 cut(s) 64, 688, 909, 924
Bme18I GGWCC 1 cut(s) 1296
BmgT120I GGNCC 2 cut(s) 896, 1296
BmiI GGNNCC 1 cut(s) 897
BmsI GCATC 2 cut(s) 510, 754
BoxI GACNNNNGTC 1 cut(s) 538
BpiI GAAGAC 3 cut(s) 372, 787, 1134
BpmI CTGGAG 2 cut(s) 725, 998
Bpu10I CCTNAGC 1 cut(s) 509
BsaBI GATNNNNATC 1 cut(s) 1272
BsaI GGTCTC 1 cut(s) 835
BsaXI ACNNNNNCTCC 2 cut(s) 19, 49
Bsc4I CCNNNNNNNGG 3 cut(s) 152, 238, 894
Bse1I ACTGG 1 cut(s) 702
Bse3DI GCAATG 2 cut(s) 11, 78
Bse8I GATNNNNATC 1 cut(s) 1272
BseGI GGATG 6 cut(s) 408, 681, 778, 1113, 1191, 1273
BseJI GATNNNNATC 1 cut(s) 1272
BseLI CCNNNNNNNGG 3 cut(s) 152, 238, 894
BseMI GCAATG 2 cut(s) 11, 78
BseMII CTCAG 2 cut(s) 523, 613
BseNI ACTGG 1 cut(s) 702
BseRI GAGGAG 2 cut(s) 281, 696
BseSI GKGCMC 1 cut(s) 847
BseXI GCAGC 3 cut(s) 673, 894, 934
Bsh1236I CGCG 2 cut(s) 65, 973
BshFI GGCC 2 cut(s) 860, 898
BsiHKAI GWGCWC 3 cut(s) 300, 741, 1161
BslFI GGGAC 1 cut(s) 19
BslI CCNNNNNNNGG 3 cut(s) 152, 238, 894
BsmAI GTCTC 2 cut(s) 421, 835
BsmFI GGGAC 1 cut(s) 19
BsnI GGCC 2 cut(s) 860, 898
Bso31I GGTCTC 1 cut(s) 835
Bsp1286I GDGCHC 4 cut(s) 300, 741, 847, 1161
Bsp143I GATC 4 cut(s) 251, 559, 1273, 1329
BspACI CCGC 6 cut(s) 63, 83, 513, 949, 971, 1038
BspANI GGCC 2 cut(s) 860, 898
BspCNI CTCAG 2 cut(s) 522, 612
BspFNI CGCG 2 cut(s) 65, 973
BspLI GGNNCC 1 cut(s) 897
BspPI GGATC 1 cut(s) 1268
BspTNI GGTCTC 1 cut(s) 835
BsrDI GCAATG 2 cut(s) 11, 78
BsrI ACTGG 1 cut(s) 702
BssMI GATC 4 cut(s) 251, 559, 1273, 1329
BssSI CACGAG 1 cut(s) 902
Bst2BI CACGAG 1 cut(s) 902
Bst4CI ACNGT 9 cut(s) 70, 346, 425, 537, 823, 837, 960, 1244, 1282
Bst6I CTCTTC 2 cut(s) 356, 974
BstC8I GCNNGC 3 cut(s) 158, 278, 1036
BstDEI CTNAG 2 cut(s) 509, 599
BstF5I GGATG 6 cut(s) 408, 681, 778, 1113, 1191, 1273
BstFNI CGCG 2 cut(s) 65, 973
BstHHI GCGC 1 cut(s) 975
BstKTI GATC 4 cut(s) 254, 562, 1276, 1332
BstMAI GTCTC 2 cut(s) 421, 835
BstMBI GATC 4 cut(s) 251, 559, 1273, 1329
BstMWI GCNNNNNNNGC 3 cut(s) 435, 866, 904
BstPAI GACNNNNGTC 1 cut(s) 538
BstSFI CTRYAG 2 cut(s) 424, 822
BstSLI GKGCMC 1 cut(s) 847
BstUI CGCG 2 cut(s) 65, 973
BstV1I GCAGC 3 cut(s) 673, 894, 934
BstV2I GAAGAC 3 cut(s) 372, 787, 1134
BstX2I RGATCY 2 cut(s) 559, 1329
BstYI RGATCY 2 cut(s) 559, 1329
BsuI GTATCC 1 cut(s) 1326
BsuRI GGCC 2 cut(s) 860, 898
BtgZI GCGATG 1 cut(s) 31
BtsCI GGATG 6 cut(s) 408, 681, 778, 1113, 1191, 1273
BtsI GCAGTG 1 cut(s) 532
BtsIMutI CAGTG 4 cut(s) 532, 819, 833, 1249
Cac8I GCNNGC 3 cut(s) 158, 278, 1036
CfoI GCGC 1 cut(s) 975
Cfr13I GGNCC 2 cut(s) 896, 1296
Csp6I GTAC 2 cut(s) 733, 851
CviAII CATG 5 cut(s) 293, 604, 649, 1031, 1293
CviQI GTAC 2 cut(s) 733, 851
DdeI CTNAG 2 cut(s) 509, 599
DpnI GATC 4 cut(s) 253, 561, 1275, 1331
DpnII GATC 4 cut(s) 251, 559, 1273, 1329
DraI TTTAAA 1 cut(s) 318
DrdI GACNNNNNNGTC 1 cut(s) 413
DseDI GACNNNNNNGTC 1 cut(s) 413
EaeI YGGCCR 1 cut(s) 858
Eam1104I CTCTTC 2 cut(s) 356, 974
EarI CTCTTC 2 cut(s) 356, 974
EciI GGCGGA 1 cut(s) 1053
Ecl136II GAGCTC 3 cut(s) 298, 739, 1159
Eco24I GRGCYC 3 cut(s) 300, 741, 1161
Eco31I GGTCTC 1 cut(s) 835
Eco47I GGWCC 1 cut(s) 1296
Eco53kI GAGCTC 3 cut(s) 298, 739, 1159
Eco57I CTGAAG 2 cut(s) 1094, 1128
EcoICRI GAGCTC 3 cut(s) 298, 739, 1159
EcoT22I ATGCAT 2 cut(s) 1262, 1294
EcoT38I GRGCYC 3 cut(s) 300, 741, 1161
FaeI CATG 5 cut(s) 296, 607, 652, 1034, 1296
FalI AAGNNNNNCTT 2 cut(s) 357, 389
FaqI GGGAC 1 cut(s) 19
FatI CATG 5 cut(s) 292, 603, 648, 1030, 1292
FblI GTMKAC 1 cut(s) 126
Fnu4HI GCNGC 4 cut(s) 63, 687, 908, 923
FokI GGATG 6 cut(s) 395, 688, 785, 1120, 1198, 1280
FriOI GRGCYC 3 cut(s) 300, 741, 1161
Fsp4HI GCNGC 4 cut(s) 63, 687, 908, 923
FspBI CTAG 1 cut(s) 327
GlaI GCGC 1 cut(s) 974
GluI GCNGC 4 cut(s) 63, 687, 908, 923
GsuI CTGGAG 2 cut(s) 725, 998
HaeIII GGCC 2 cut(s) 860, 898
HhaI GCGC 1 cut(s) 975
Hin1II CATG 5 cut(s) 296, 607, 652, 1034, 1296
Hin6I GCGC 1 cut(s) 973
HinP1I GCGC 1 cut(s) 973
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HindIII AAGCTT 2 cut(s) 867, 1098
HinfI GANTC 7 cut(s) 32, 162, 258, 480, 570, 1305, 1340
HphI GGTGA 1 cut(s) 960
Hpy166II GTNNAC 2 cut(s) 127, 574
Hpy188I TCNGA 9 cut(s) 263, 675, 755, 772, 919, 1074, 1223, 1267, 1300
Hpy188III TCNNGA 3 cut(s) 255, 1003, 1252
Hpy8I GTNNAC 2 cut(s) 127, 574
Hpy99I CGWCG 1 cut(s) 1325
HpyAV CCTTC 5 cut(s) 380, 1000, 1035, 1176, 1228
HpyCH4III ACNGT 9 cut(s) 70, 346, 425, 537, 823, 837, 960, 1244, 1282
HpyCH4V TGCA 5 cut(s) 11, 525, 1247, 1260, 1292
HpyF10VI GCNNNNNNNGC 3 cut(s) 435, 866, 904
HpyF3I CTNAG 2 cut(s) 509, 599
Hsp92II CATG 5 cut(s) 296, 607, 652, 1034, 1296
HspAI GCGC 1 cut(s) 973
Kzo9I GATC 4 cut(s) 251, 559, 1273, 1329
LmnI GCTCC 3 cut(s) 303, 683, 744
Lsp1109I GCAGC 3 cut(s) 673, 894, 934
LweI GCATC 2 cut(s) 510, 754
MaeI CTAG 1 cut(s) 327
MaeIII GTNAC 3 cut(s) 451, 613, 1238
MalI GATC 4 cut(s) 253, 561, 1275, 1331
MboI GATC 4 cut(s) 251, 559, 1273, 1329
MflI RGATCY 2 cut(s) 559, 1329
MhlI GDGCHC 4 cut(s) 300, 741, 847, 1161
MlsI TGGCCA 1 cut(s) 860
MluCI AATT 5 cut(s) 98, 195, 226, 876, 997
MluNI TGGCCA 1 cut(s) 860
MlyI GAGTC 1 cut(s) 579
MmeI TCCRAC 3 cut(s) 419, 1301, 1323
MnlI CCTC 7 cut(s) 156, 215, 259, 312, 518, 674, 1165
Mox20I TGGCCA 1 cut(s) 860
Mph1103I ATGCAT 2 cut(s) 1262, 1294
MroXI GAANNNNTTC 2 cut(s) 345, 369
MscI TGGCCA 1 cut(s) 860
MseI TTAA 3 cut(s) 317, 872, 879
Msp20I TGGCCA 1 cut(s) 860
MspA1I CMGCKG 1 cut(s) 513
MvnI CGCG 2 cut(s) 65, 973
MwoI GCNNNNNNNGC 3 cut(s) 435, 866, 904
NdeII GATC 4 cut(s) 251, 559, 1273, 1329
NlaIII CATG 5 cut(s) 296, 607, 652, 1034, 1296
NlaIV GGNNCC 1 cut(s) 897
NsiI ATGCAT 2 cut(s) 1262, 1294
PdmI GAANNNNTTC 2 cut(s) 345, 369
PfeI GAWTC 6 cut(s) 32, 162, 258, 480, 1305, 1340
PkrI GCNGC 4 cut(s) 64, 688, 909, 924
PleI GAGTC 1 cut(s) 578
PpsI GAGTC 1 cut(s) 578
PshAI GACNNNNGTC 1 cut(s) 538
Psp124BI GAGCTC 3 cut(s) 300, 741, 1161
PspN4I GGNNCC 1 cut(s) 897
PspPI GGNCC 2 cut(s) 896, 1296
PsuI RGATCY 2 cut(s) 559, 1329
RsaI GTAC 2 cut(s) 734, 852
RsaNI GTAC 2 cut(s) 733, 851
SacI GAGCTC 3 cut(s) 300, 741, 1161
SalI GTCGAC 1 cut(s) 125
SaqAI TTAA 3 cut(s) 317, 872, 879
SatI GCNGC 4 cut(s) 63, 687, 908, 923
Sau3AI GATC 4 cut(s) 251, 559, 1273, 1329
Sau96I GGNCC 2 cut(s) 896, 1296
SchI GAGTC 1 cut(s) 579
SduI GDGCHC 4 cut(s) 300, 741, 847, 1161
SfaNI GCATC 2 cut(s) 510, 754
SfcI CTRYAG 2 cut(s) 424, 822
SinI GGWCC 1 cut(s) 1296
Sse9I AATT 5 cut(s) 98, 195, 226, 876, 997
SsiI CCGC 6 cut(s) 63, 83, 513, 949, 971, 1038
SspMI CTAG 1 cut(s) 327
SstI GAGCTC 3 cut(s) 300, 741, 1161
TaaI ACNGT 9 cut(s) 70, 346, 425, 537, 823, 837, 960, 1244, 1282
TaqI TCGA 4 cut(s) 126, 363, 542, 1323
TasI AATT 5 cut(s) 98, 195, 226, 876, 997
TauI GCSGC 1 cut(s) 65
TfiI GAWTC 6 cut(s) 32, 162, 258, 480, 1305, 1340
Tru1I TTAA 3 cut(s) 317, 872, 879
Tru9I TTAA 3 cut(s) 317, 872, 879
TscAI CASTG 4 cut(s) 532, 826, 840, 1249
TseI GCWGC 3 cut(s) 686, 907, 922
TspDTI ATGAA 7 cut(s) 228, 260, 792, 825, 1019, 1125, 1202
TspGWI ACGGA 4 cut(s) 44, 435, 439, 473
TspRI CASTG 4 cut(s) 532, 826, 840, 1249
VpaK11BI GGWCC 1 cut(s) 1296
XapI RAATTY 4 cut(s) 98, 195, 226, 997
XmiI GTMKAC 1 cut(s) 126
XmnI GAANNNNTTC 2 cut(s) 345, 369
XspI CTAG 1 cut(s) 327
Zsp2I ATGCAT 2 cut(s) 1262, 1294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.