Rw7G029430

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0008224
Physical Location & Seq
Forward (+)
40845850 .. 40847214
1365 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G029430.1

Sequence Viewer

Length: 1140 bp
ATGGAGACTTGGCTGCTTAGACCATTCTCATCTGTCACTACAGAATCATTTCCGATTTCCTTCACTCATTTGTTAGCTGGAACTACTTTGATCATTGCAGTTCTCCTTTTAAAATTCACATCCAAGAGGATATATCTAATTGATTTCACCTGCTATCTGCCTCCTGGTAGATTGCGGGTTCCAAAGTCGCACTTCAACGAACATGTGGAGCTGTCTAACGTCAATGGCAGAGAGGGTATCGACTTTCATGTCAAGGTATTGGAAAGATCAGGCATTGGATCAGGGACTTGCACGCCTACTTCATTGCACGAGATCCCGCCTCGTGGGTCTTTGGATGATACTCGAAAAGAAGTTGAAATGGTTCTCTTCACTGTTGTGGAAGACCTTCTTTCCAAGCACAAATCAAACTGCAGCATTTTCTGCTCAACACCATCCATTAGTTCTATGATCATAAACAAAAAAAAGTCAATGCTTCTTCCAAACATCTTGTTTCGGATGGGAGGAGCTGCTATTCTACTATCGAATAGGAAGCAAGACAAGGGAATGGCAAAGTATGAGCTTGAGCTTCTTGTTCAAGCCCACATTGGTTCGGATGACGAAGCATACCAATCTGTTTTCCAGAAATCAGATGAAGATGGAGTTGAAGGACTTGAAGGGGTCTCACTATCAAGAGCCCTACTAAATGTGGCAACAAAAGCTCTAAAAACAAACATATCATCTCTGGGGCCACTTGTGCTACCACACTCCGATCGAGCAGCTTCGGTTCTCATGAAGGGAGCTTATGTGCCAAATTTCAAGAAGGGGATCCAACATTTCTGTATACATGCTGGTGGAAGAGCAGTGATCGATGGCCTAGAGCAAAGTCTGAAACTGCAGAAGGAAGATGGAGAGGCTTCAAGAATGACATTACATAGATTTGGAAACACATCATCTTCTTCAATCTGGTATGAGCTTTGCTATTTGGAAACAAAAGGAAGGGTGAACAAAAGAAACAAAGTTTGGCAAATTGCATTTGGAAGTGGCTTCAAGTGTAACAGTGCAGTTTGGAAATGCATTTCAGATGTGGATCCAACAAAAAGAAATGCATGGTCAGACAGCTTCAATTTGTATCTTATTATTGATGATGTGATCCCAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

379

Amino Acids

42.13

Weight (kDa)

8.78

Isoelectric Point (pI)

32.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 40 - 153 1.2e-26 FAE1/Type III polyketide synthase-like protein
FAE1_CUT1_RppA PF08392 153 - 253 8.4e-34 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 268 - 350 5.1e-11 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 272 - 348 8.9e-10 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 158
AasI GACNNNNNNGTC 1 cut(s) 248
Acc36I ACCTGC 1 cut(s) 158
AccI GTMKAC 1 cut(s) 820
AciI CCGC 2 cut(s) 175, 317
AclWI GGATC 7 cut(s) 286, 307, 799, 812, 1061, 1074, 1123
AcsI RAATTY 2 cut(s) 113, 790
AfiI CCNNNNNNNGG 1 cut(s) 323
AflIII ACRYGT 1 cut(s) 202
AjnI CCWGG 1 cut(s) 163
AjuI GAANNNNNNNTTGG 2 cut(s) 982, 1014
AleI CACNNNNGTG 1 cut(s) 374
Alw26I GTCTC 1 cut(s) 664
AlwI GGATC 7 cut(s) 286, 307, 799, 812, 1061, 1074, 1123
AoxI GGCC 2 cut(s) 725, 850
ApeKI GCWGC 4 cut(s) 13, 411, 506, 755
ApoI RAATTY 2 cut(s) 113, 790
Asp700I GAANNNNTTC 3 cut(s) 48, 360, 384
AspS9I GGNCC 1 cut(s) 725
AsuHPI GGTGA 2 cut(s) 139, 991
BamHI GGATCC 2 cut(s) 804, 1066
BanII GRGCYC 1 cut(s) 676
BauI CACGAG 2 cut(s) 308, 321
BbsI GAAGAC 1 cut(s) 387
BbvI GCAGC 3 cut(s) 423, 493, 767
BccI CCATC 5 cut(s) 439, 490, 629, 842, 878
BciT130I CCWGG 1 cut(s) 165
BclI TGATCA 2 cut(s) 90, 447
BcoDI GTCTC 1 cut(s) 664
BfaI CTAG 1 cut(s) 854
BfmI CTRYAG 3 cut(s) 39, 409, 872
BfuAI ACCTGC 1 cut(s) 158
BisI GCNGC 4 cut(s) 14, 412, 507, 756
BlsI GCNGC 4 cut(s) 15, 413, 508, 757
Bme1390I CCNGG 1 cut(s) 165
BmgT120I GGNCC 1 cut(s) 725
BmiI GGNNCC 4 cut(s) 180, 726, 806, 1068
BmrFI CCNGG 1 cut(s) 165
BpiI GAAGAC 1 cut(s) 387
BpuEI CTTGAG 1 cut(s) 581
Bsa29I ATCGAT 1 cut(s) 846
BsaBI GATNNNNATC 1 cut(s) 1065
BsaI GGTCTC 1 cut(s) 664
BsaXI ACNNNNNCTCC 2 cut(s) 768, 798
Bsc4I CCNNNNNNNGG 1 cut(s) 323
Bse3DI GCAATG 2 cut(s) 93, 302
Bse8I GATNNNNATC 1 cut(s) 1065
BseBI CCWGG 1 cut(s) 165
BseCI ATCGAT 1 cut(s) 846
BseGI GGATG 5 cut(s) 119, 340, 431, 501, 598
BseJI GATNNNNATC 1 cut(s) 1065
BseLI CCNNNNNNNGG 1 cut(s) 323
BseMI GCAATG 2 cut(s) 93, 302
BseRI GAGGAG 1 cut(s) 516
BseXI GCAGC 3 cut(s) 423, 493, 767
BsgI GTGCAG 1 cut(s) 1059
Bsh1285I CGRYCG 1 cut(s) 751
BshFI GGCC 2 cut(s) 727, 852
BshVI ATCGAT 1 cut(s) 846
BsiEI CGRYCG 1 cut(s) 751
BslFI GGGAC 1 cut(s) 298
BslI CCNNNNNNNGG 1 cut(s) 323
BsmAI GTCTC 1 cut(s) 664
BsmFI GGGAC 1 cut(s) 298
BsnI GGCC 2 cut(s) 727, 852
Bso31I GGTCTC 1 cut(s) 664
Bsp1286I GDGCHC 1 cut(s) 676
BspACI CCGC 2 cut(s) 175, 317
BspANI GGCC 2 cut(s) 727, 852
BspDI ATCGAT 1 cut(s) 846
BspHI TCATGA 1 cut(s) 768
BspLI GGNNCC 4 cut(s) 180, 726, 806, 1068
BspMAI CTGCAG 2 cut(s) 413, 876
BspMI ACCTGC 1 cut(s) 158
BspPI GGATC 7 cut(s) 286, 307, 799, 812, 1061, 1074, 1123
BspQI GCTCTTC 1 cut(s) 829
BspTNI GGTCTC 1 cut(s) 664
BsrDI GCAATG 2 cut(s) 93, 302
BssNAI GTATAC 1 cut(s) 821
BssSI CACGAG 2 cut(s) 308, 321
Bst1107I GTATAC 1 cut(s) 821
Bst2BI CACGAG 2 cut(s) 308, 321
Bst2UI CCWGG 1 cut(s) 165
Bst4CI ACNGT 2 cut(s) 373, 1037
Bst6I CTCTTC 2 cut(s) 371, 829
BstAPI GCANNNNNTGC 1 cut(s) 420
BstC8I GCNNGC 1 cut(s) 293
BstDEI CTNAG 1 cut(s) 17
BstF5I GGATG 5 cut(s) 119, 340, 431, 501, 598
BstMAI GTCTC 1 cut(s) 664
BstMCI CGRYCG 1 cut(s) 751
BstMWI GCNNNNNNNGC 3 cut(s) 420, 695, 733
BstNI CCWGG 1 cut(s) 165
BstNSI RCATGY 2 cut(s) 206, 827
BstSCI CCNGG 1 cut(s) 163
BstSFI CTRYAG 3 cut(s) 39, 409, 872
BstV1I GCAGC 3 cut(s) 423, 493, 767
BstV2I GAAGAC 1 cut(s) 387
BstX2I RGATCY 3 cut(s) 312, 804, 1066
BstYI RGATCY 3 cut(s) 312, 804, 1066
BstZ17I GTATAC 1 cut(s) 821
Bsu15I ATCGAT 1 cut(s) 846
BsuRI GGCC 2 cut(s) 727, 852
BsuTUI ATCGAT 1 cut(s) 846
BtsCI GGATG 5 cut(s) 119, 340, 431, 501, 598
BtsI GCAGTG 1 cut(s) 846
BtsIMutI CAGTG 3 cut(s) 369, 846, 1042
BveI ACCTGC 1 cut(s) 158
Cac8I GCNNGC 1 cut(s) 293
CciI TCATGA 1 cut(s) 768
Cfr13I GGNCC 1 cut(s) 725
ClaI ATCGAT 1 cut(s) 846
CviAII CATG 5 cut(s) 203, 248, 769, 824, 1086
DdeI CTNAG 1 cut(s) 17
DraI TTTAAA 1 cut(s) 111
DrdI GACNNNNNNGTC 1 cut(s) 248
DseDI GACNNNNNNGTC 1 cut(s) 248
Eam1104I CTCTTC 2 cut(s) 371, 829
EarI CTCTTC 2 cut(s) 371, 829
Eco24I GRGCYC 1 cut(s) 676
Eco31I GGTCTC 1 cut(s) 664
EcoRII CCWGG 1 cut(s) 163
EcoT22I ATGCAT 2 cut(s) 1055, 1087
EcoT38I GRGCYC 1 cut(s) 676
FaeI CATG 5 cut(s) 206, 251, 772, 827, 1089
FalI AAGNNNNNCTT 4 cut(s) 176, 208, 372, 404
FaqI GGGAC 1 cut(s) 298
FatI CATG 5 cut(s) 202, 247, 768, 823, 1085
FauI CCCGC 2 cut(s) 168, 324
FbaI TGATCA 2 cut(s) 90, 447
FblI GTMKAC 1 cut(s) 820
Fnu4HI GCNGC 4 cut(s) 14, 412, 507, 756
FokI GGATG 5 cut(s) 106, 347, 418, 508, 605
FriOI GRGCYC 1 cut(s) 676
Fsp4HI GCNGC 4 cut(s) 14, 412, 507, 756
FspBI CTAG 1 cut(s) 854
GluI GCNGC 4 cut(s) 14, 412, 507, 756
HaeIII GGCC 2 cut(s) 727, 852
Hin1II CATG 5 cut(s) 206, 251, 772, 827, 1089
HinfI GANTC 1 cut(s) 44
HphI GGTGA 2 cut(s) 139, 991
Hpy166II GTNNAC 2 cut(s) 821, 982
Hpy188I TCNGA 8 cut(s) 54, 495, 592, 628, 748, 867, 1060, 1093
Hpy188III TCNNGA 5 cut(s) 619, 669, 769, 796, 897
Hpy8I GTNNAC 2 cut(s) 821, 982
HpyAV CCTTC 8 cut(s) 70, 395, 638, 647, 766, 793, 871, 969
HpyCH4III ACNGT 2 cut(s) 373, 1037
HpyCH4IV ACGT 1 cut(s) 219
HpyCH4V TGCA 9 cut(s) 98, 291, 307, 411, 874, 1010, 1040, 1053, 1085
HpyF10VI GCNNNNNNNGC 3 cut(s) 420, 695, 733
HpyF3I CTNAG 1 cut(s) 17
HpySE526I ACGT 1 cut(s) 219
Hsp92II CATG 5 cut(s) 206, 251, 772, 827, 1089
Ksp22I TGATCA 2 cut(s) 90, 447
LguI GCTCTTC 1 cut(s) 829
LmnI GCTCC 3 cut(s) 208, 503, 776
Lsp1109I GCAGC 3 cut(s) 423, 493, 767
MaeI CTAG 1 cut(s) 854
MaeII ACGT 1 cut(s) 219
MaeIII GTNAC 2 cut(s) 34, 1031
MboII GAAGA 8 cut(s) 358, 392, 467, 644, 846, 893, 924, 927
MflI RGATCY 3 cut(s) 312, 804, 1066
MhlI GDGCHC 1 cut(s) 676
MluCI AATT 5 cut(s) 113, 138, 790, 1005, 1102
MmeI TCCRAC 2 cut(s) 832, 1094
MnlI CCTC 6 cut(s) 120, 171, 226, 330, 494, 883
Mph1103I ATGCAT 2 cut(s) 1055, 1087
MroXI GAANNNNTTC 3 cut(s) 48, 360, 384
MseI TTAA 1 cut(s) 110
MslI CAYNNNNRTG 2 cut(s) 374, 828
MspR9I CCNGG 1 cut(s) 165
MvaI CCWGG 1 cut(s) 165
MwoI GCNNNNNNNGC 3 cut(s) 420, 695, 733
NlaIII CATG 5 cut(s) 206, 251, 772, 827, 1089
NlaIV GGNNCC 4 cut(s) 180, 726, 806, 1068
NmuCI GTSAC 1 cut(s) 34
NsiI ATGCAT 2 cut(s) 1055, 1087
NspI RCATGY 2 cut(s) 206, 827
OliI CACNNNNGTG 1 cut(s) 374
PagI TCATGA 1 cut(s) 768
PaqCI CACCTGC 1 cut(s) 158
PciI ACATGT 1 cut(s) 202
PciSI GCTCTTC 1 cut(s) 829
PdmI GAANNNNTTC 3 cut(s) 48, 360, 384
PfeI GAWTC 1 cut(s) 44
PkrI GCNGC 4 cut(s) 15, 413, 508, 757
Ple19I CGATCG 1 cut(s) 751
PscI ACATGT 1 cut(s) 202
Psp6I CCWGG 1 cut(s) 163
PspGI CCWGG 1 cut(s) 163
PspN4I GGNNCC 4 cut(s) 180, 726, 806, 1068
PspPI GGNCC 1 cut(s) 725
PstI CTGCAG 2 cut(s) 413, 876
PsuI RGATCY 3 cut(s) 312, 804, 1066
PvuI CGATCG 1 cut(s) 751
RseI CAYNNNNRTG 2 cut(s) 374, 828
SapI GCTCTTC 1 cut(s) 829
SaqAI TTAA 1 cut(s) 110
SatI GCNGC 4 cut(s) 14, 412, 507, 756
Sau96I GGNCC 1 cut(s) 725
ScrFI CCNGG 1 cut(s) 165
SduI GDGCHC 1 cut(s) 676
SfcI CTRYAG 3 cut(s) 39, 409, 872
SmiMI CAYNNNNRTG 2 cut(s) 374, 828
SmlI CTYRAG 1 cut(s) 560
SmoI CTYRAG 1 cut(s) 560
Sse9I AATT 5 cut(s) 113, 138, 790, 1005, 1102
SsiI CCGC 2 cut(s) 175, 317
SspMI CTAG 1 cut(s) 854
StyD4I CCNGG 1 cut(s) 163
TaaI ACNGT 2 cut(s) 373, 1037
TaiI ACGT 1 cut(s) 222
TaqI TCGA 5 cut(s) 240, 343, 521, 751, 846
TasI AATT 5 cut(s) 113, 138, 790, 1005, 1102
TfiI GAWTC 1 cut(s) 44
Tru1I TTAA 1 cut(s) 110
Tru9I TTAA 1 cut(s) 110
TscAI CASTG 3 cut(s) 376, 846, 1042
TseFI GTSAC 1 cut(s) 34
TseI GCWGC 4 cut(s) 13, 411, 506, 755
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 4 cut(s) 236, 291, 645, 785
TspRI CASTG 3 cut(s) 376, 846, 1042
XapI RAATTY 2 cut(s) 113, 790
XceI RCATGY 2 cut(s) 206, 827
XmiI GTMKAC 1 cut(s) 820
XmnI GAANNNNTTC 3 cut(s) 48, 360, 384
XspI CTAG 1 cut(s) 854
Zsp2I ATGCAT 2 cut(s) 1055, 1087
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.