MD08G1230500.v1.1

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008224
Physical Location & Seq
Reverse (-)
29609433 .. 29610803
1371 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1230500.v1.1.491

Sequence Viewer

Length: 1371 bp
ATGGAGACCGCATTGTTTGCATCTTTCTCATCTATCACCACAGAATTATCTCCAATCTCCCTCACCCATTTCTTAGCTACAACTGTCTTGACCACTGCCCTAGTTCTCTATTTTCTTTTACGATCCAAGAGTATATACCTTGTCGATTTCACCTGCTACCTGCCTCCTGAGAACCTGCGGGTTCCAGCTTCAAAATTCATCGAACACATTGAAATATCGGGAATTCATGATAGAGAAAGTGTCAACTTCCAAGCCAAGGTATTGGAAAGATCAGGCATCGGATCGGAGAGTTGCATGCCTGTTACAGTGCATGAGATCCCATCTTATTCTTCATTGGAAGCTACTAGGAAAGAAACAGAACTGGTTCTCTTCACCGTTGTTGAAGAGCTTCTTTCCAAACACAACATAAATCCGAAAAGCATTGACATTCTTGTATCGAACTGCAGCCTTTTCTGCCCTACGCCGTCCATTTCATCAATGATAATTAACAAGTTTGGATTCCGAAGCAGCATAAAGAGCATCAGCCTCGGTGGAATGGGTTGTAGTGCGGGAATCTTGTCGATAAGTTTGGCTAAAGATCTTCTAAAAGTTCACAAGAATTCACTGGCTTTAGTTCTCAGCATGGAAGCTGTAACTCCCAACGGTTATAATGGTAAAAGAAAATCAATGCTTCTTCCAAACATTTTGTTCCGAATGGGAGGAGCTGCTATCCTACTATCAAATAGGAAACAAGACAAGGGAATAGCTAAGTACGAGCTCCAGCATCTTGTTCGAACCCACATTGGTTCGGATGACCAAGCATACGAATCTGTTTTCCAGCAACCTGATGAAGCAGGACATGTAGGGGTCTCACTGTCGCGGGAACTTGTACATGTGGCCACCAAAGCTCTGAGAATTAACTTATCAGAGTTGGGGCCACTTGTGCTACCATATTCTGAGCAGCTTCGATATGGATGGTCAATGATCCACAAGAAGCTTTGGTTTTTACCAAAGCAAAAGGCAATTTATGTTCCGAAATTCAACAAAGCTCTCCAGCAGTTCTGTATACATGCCGGTGGAAGGGCGGTGATTGATGGAATTGTAGATAGTCTCAAGCTGCAGAAAGAAGATGGAGAAGCTTCGAGGATGACGTTACACAGATTTGGCAACACTTCATCATCTTCGGTGTGGTACGAGCTATGCTACTTGGAGGCAAAAGGAAGGGTGAAGAAAGGAAACCGAGTTTGGCAAATTGCGTTTGGAAGTGGCTTCAAGTGTAACAGTGCAATCTGGAAATGCATTGCGGATATTGATCGGACAGAAAGAAATGCATGGTCGGACAGCATCCATTCGTATCCCATTGACGATGATGCCCCAAATGTAACCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

457

Amino Acids

50.69

Weight (kDa)

8.88

Isoelectric Point (pI)

43.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 38 - 326 1.3e-121 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 344 - 425 5.2e-12 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 347 - 420 1.8e-08 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 648
AarI CACCTGC 1 cut(s) 161
Acc36I ACCTGC 3 cut(s) 161, 168, 183
AccI GTMKAC 1 cut(s) 1045
AccII CGCG 1 cut(s) 859
AciI CCGC 6 cut(s) 9, 178, 548, 859, 1064, 1283
AclWI GGATC 4 cut(s) 117, 289, 310, 958
AcoI YGGCCR 1 cut(s) 876
AcsI RAATTY 4 cut(s) 194, 222, 598, 1016
AfaI GTAC 3 cut(s) 752, 870, 1172
AfiI CCNNNNNNNGG 2 cut(s) 256, 1059
AflIII ACRYGT 2 cut(s) 838, 871
AgsI TTSAA 5 cut(s) 192, 212, 383, 1021, 1252
AjuI GAANNNNNNNTTGG 2 cut(s) 1207, 1239
Alw21I GWGCWC 1 cut(s) 759
Alw26I GTCTC 2 cut(s) 853, 1094
AlwI GGATC 4 cut(s) 117, 289, 310, 958
AoxI GGCC 2 cut(s) 876, 914
ApeKI GCWGC 5 cut(s) 444, 507, 704, 940, 1096
ApoI RAATTY 4 cut(s) 194, 222, 598, 1016
Asp700I GAANNNNTTC 2 cut(s) 363, 387
AspS9I GGNCC 1 cut(s) 914
AsuHPI GGTGA 6 cut(s) 28, 55, 142, 364, 1078, 1216
AsuII TTCGAA 1 cut(s) 772
BalI TGGCCA 1 cut(s) 878
BanII GRGCYC 1 cut(s) 759
Bbv12I GWGCWC 1 cut(s) 759
BbvI GCAGC 5 cut(s) 456, 519, 691, 952, 1083
BccI CCATC 4 cut(s) 328, 948, 1067, 1103
BceAI ACGGC 1 cut(s) 448
BcgI CGANNNNNNTGC 4 cut(s) 508, 542, 752, 786
BciVI GTATCC 1 cut(s) 1344
BcoDI GTCTC 2 cut(s) 853, 1094
BfaI CTAG 2 cut(s) 101, 345
BfmI CTRYAG 2 cut(s) 442, 1097
BfuAI ACCTGC 3 cut(s) 161, 168, 183
BfuI GTATCC 1 cut(s) 1344
BglII AGATCT 1 cut(s) 577
BisI GCNGC 5 cut(s) 445, 508, 705, 941, 1097
BlsI GCNGC 5 cut(s) 446, 509, 706, 942, 1098
BmgT120I GGNCC 1 cut(s) 914
BmiI GGNNCC 2 cut(s) 183, 915
BmsI GCATC 6 cut(s) 29, 285, 528, 772, 1332, 1339
BpmI CTGGAG 2 cut(s) 743, 1016
Bpu14I TTCGAA 1 cut(s) 772
BpuEI CTTGAG 1 cut(s) 1076
BsaBI GATNNNNATC 1 cut(s) 1290
BsaI GGTCTC 1 cut(s) 853
BsaJI CCNNGG 2 cut(s) 255, 526
Bsc4I CCNNNNNNNGG 2 cut(s) 256, 1059
Bse118I RCCGGY 1 cut(s) 1052
Bse1I ACTGG 2 cut(s) 366, 609
Bse3DI GCAATG 1 cut(s) 1278
Bse8I GATNNNNATC 1 cut(s) 1290
BseDI CCNNGG 2 cut(s) 255, 526
BseGI GGATG 4 cut(s) 796, 959, 1131, 1323
BseJI GATNNNNATC 1 cut(s) 1290
BseLI CCNNNNNNNGG 2 cut(s) 256, 1059
BseMI GCAATG 1 cut(s) 1278
BseMII CTCAG 4 cut(s) 159, 631, 881, 927
BseNI ACTGG 2 cut(s) 366, 609
BseRI GAGGAG 1 cut(s) 714
BseXI GCAGC 5 cut(s) 456, 519, 691, 952, 1083
Bsh1236I CGCG 1 cut(s) 859
BshFI GGCC 2 cut(s) 878, 916
BsiHKAI GWGCWC 1 cut(s) 759
BsiSI CCGG 1 cut(s) 1053
BslI CCNNNNNNNGG 2 cut(s) 256, 1059
BsmAI GTCTC 2 cut(s) 853, 1094
BsnI GGCC 2 cut(s) 878, 916
Bso31I GGTCTC 1 cut(s) 853
Bsp119I TTCGAA 1 cut(s) 772
Bsp1286I GDGCHC 1 cut(s) 759
Bsp1407I TGTACA 1 cut(s) 868
Bsp143I GATC 7 cut(s) 122, 269, 281, 315, 577, 963, 1291
BspACI CCGC 6 cut(s) 9, 178, 548, 859, 1064, 1283
BspANI GGCC 2 cut(s) 878, 916
BspCNI CTCAG 4 cut(s) 160, 630, 882, 928
BspFNI CGCG 1 cut(s) 859
BspHI TCATGA 1 cut(s) 226
BspLI GGNNCC 2 cut(s) 183, 915
BspMAI CTGCAG 2 cut(s) 446, 1101
BspMI ACCTGC 3 cut(s) 161, 168, 183
BspPI GGATC 4 cut(s) 117, 289, 310, 958
BspQI GCTCTTC 1 cut(s) 378
BspT104I TTCGAA 1 cut(s) 772
BspTNI GGTCTC 1 cut(s) 853
BsrDI GCAATG 1 cut(s) 1278
BsrFI RCCGGY 1 cut(s) 1052
BsrGI TGTACA 1 cut(s) 868
BsrI ACTGG 2 cut(s) 366, 609
BssAI RCCGGY 1 cut(s) 1052
BssECI CCNNGG 2 cut(s) 255, 526
BssMI GATC 7 cut(s) 122, 269, 281, 315, 577, 963, 1291
BssNAI GTATAC 1 cut(s) 1046
BssT1I CCWWGG 1 cut(s) 255
Bst1107I GTATAC 1 cut(s) 1046
Bst4CI ACNGT 6 cut(s) 85, 307, 376, 644, 855, 1262
Bst6I CTCTTC 2 cut(s) 374, 378
BstAPI GCANNNNNTGC 1 cut(s) 17
BstAUI TGTACA 1 cut(s) 868
BstBI TTCGAA 1 cut(s) 772
BstC8I GCNNGC 1 cut(s) 296
BstDEI CTNAG 6 cut(s) 73, 168, 617, 747, 890, 936
BstF5I GGATG 4 cut(s) 796, 959, 1131, 1323
BstFNI CGCG 1 cut(s) 859
BstKTI GATC 7 cut(s) 125, 272, 284, 318, 580, 966, 1294
BstMAI GTCTC 2 cut(s) 853, 1094
BstMBI GATC 7 cut(s) 122, 269, 281, 315, 577, 963, 1291
BstMWI GCNNNNNNNGC 5 cut(s) 17, 453, 516, 884, 922
BstNSI RCATGY 4 cut(s) 298, 842, 875, 1052
BstSFI CTRYAG 2 cut(s) 442, 1097
BstUI CGCG 1 cut(s) 859
BstV1I GCAGC 5 cut(s) 456, 519, 691, 952, 1083
BstX2I RGATCY 2 cut(s) 315, 577
BstXI CCANNNNNNTGG 1 cut(s) 262
BstYI RGATCY 2 cut(s) 315, 577
BstZ17I GTATAC 1 cut(s) 1046
BsuI GTATCC 1 cut(s) 1344
BsuRI GGCC 2 cut(s) 878, 916
BtsCI GGATG 4 cut(s) 796, 959, 1131, 1323
BtsI GCAGTG 1 cut(s) 93
BtsIMutI CAGTG 5 cut(s) 93, 312, 602, 851, 1267
BveI ACCTGC 3 cut(s) 161, 168, 183
Cac8I GCNNGC 1 cut(s) 296
CciI TCATGA 1 cut(s) 226
Cfr10I RCCGGY 1 cut(s) 1052
Cfr13I GGNCC 1 cut(s) 914
Csp6I GTAC 3 cut(s) 751, 869, 1171
CviAII CATG 8 cut(s) 227, 295, 311, 622, 839, 872, 1049, 1311
CviQI GTAC 3 cut(s) 751, 869, 1171
DdeI CTNAG 6 cut(s) 73, 168, 617, 747, 890, 936
DpnI GATC 7 cut(s) 124, 271, 283, 317, 579, 965, 1293
DpnII GATC 7 cut(s) 122, 269, 281, 315, 577, 963, 1291
EaeI YGGCCR 1 cut(s) 876
Eam1104I CTCTTC 2 cut(s) 374, 378
EarI CTCTTC 2 cut(s) 374, 378
Ecl136II GAGCTC 1 cut(s) 757
Eco130I CCWWGG 1 cut(s) 255
Eco24I GRGCYC 1 cut(s) 759
Eco31I GGTCTC 1 cut(s) 853
Eco53kI GAGCTC 1 cut(s) 757
EcoICRI GAGCTC 1 cut(s) 757
EcoRI GAATTC 2 cut(s) 222, 598
EcoT14I CCWWGG 1 cut(s) 255
EcoT22I ATGCAT 2 cut(s) 1280, 1312
EcoT38I GRGCYC 1 cut(s) 759
ErhI CCWWGG 1 cut(s) 255
FaeI CATG 8 cut(s) 230, 298, 314, 625, 842, 875, 1052, 1314
FalI AAGNNNNNCTT 2 cut(s) 375, 407
FatI CATG 8 cut(s) 226, 294, 310, 621, 838, 871, 1048, 1310
FauI CCCGC 3 cut(s) 171, 541, 852
FblI GTMKAC 1 cut(s) 1045
Fnu4HI GCNGC 5 cut(s) 445, 508, 705, 941, 1097
FokI GGATG 4 cut(s) 803, 966, 1138, 1310
FriOI GRGCYC 1 cut(s) 759
Fsp4HI GCNGC 5 cut(s) 445, 508, 705, 941, 1097
FspBI CTAG 2 cut(s) 101, 345
GluI GCNGC 5 cut(s) 445, 508, 705, 941, 1097
GsuI CTGGAG 2 cut(s) 743, 1016
HaeIII GGCC 2 cut(s) 878, 916
HapII CCGG 1 cut(s) 1053
Hin1II CATG 8 cut(s) 230, 298, 314, 625, 842, 875, 1052, 1314
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HindIII AAGCTT 2 cut(s) 974, 1116
HinfI GANTC 3 cut(s) 498, 552, 806
HpaII CCGG 1 cut(s) 1053
HphI GGTGA 6 cut(s) 28, 55, 142, 364, 1078, 1216
Hpy166II GTNNAC 3 cut(s) 244, 592, 1046
Hpy188III TCNNGA 5 cut(s) 88, 167, 219, 227, 1270
Hpy8I GTNNAC 3 cut(s) 244, 592, 1046
HpyAV CCTTC 2 cut(s) 1053, 1194
HpyCH4III ACNGT 6 cut(s) 85, 307, 376, 644, 855, 1262
HpyCH4IV ACGT 1 cut(s) 1130
HpyCH4V TGCA 8 cut(s) 20, 294, 310, 444, 1099, 1265, 1278, 1310
HpyF10VI GCNNNNNNNGC 5 cut(s) 17, 453, 516, 884, 922
HpyF3I CTNAG 6 cut(s) 73, 168, 617, 747, 890, 936
HpySE526I ACGT 1 cut(s) 1130
Hsp92II CATG 8 cut(s) 230, 298, 314, 625, 842, 875, 1052, 1314
Kzo9I GATC 7 cut(s) 122, 269, 281, 315, 577, 963, 1291
LguI GCTCTTC 1 cut(s) 378
LmnI GCTCC 2 cut(s) 701, 762
Lsp1109I GCAGC 5 cut(s) 456, 519, 691, 952, 1083
LweI GCATC 6 cut(s) 29, 285, 528, 772, 1332, 1339
MaeI CTAG 2 cut(s) 101, 345
MaeII ACGT 1 cut(s) 1130
MaeIII GTNAC 5 cut(s) 301, 631, 1131, 1256, 1360
MalI GATC 7 cut(s) 124, 271, 283, 317, 579, 965, 1293
MboI GATC 7 cut(s) 122, 269, 281, 315, 577, 963, 1291
MboII GAAGA 8 cut(s) 321, 361, 395, 572, 665, 1118, 1152, 1219
MflI RGATCY 2 cut(s) 315, 577
MhlI GDGCHC 1 cut(s) 759
MlsI TGGCCA 1 cut(s) 878
MluNI TGGCCA 1 cut(s) 878
MmeI TCCRAC 1 cut(s) 1296
MnlI CCTC 6 cut(s) 71, 174, 536, 692, 1116, 1183
Mox20I TGGCCA 1 cut(s) 878
Mph1103I ATGCAT 2 cut(s) 1280, 1312
MroXI GAANNNNTTC 2 cut(s) 363, 387
MscI TGGCCA 1 cut(s) 878
MseI TTAA 2 cut(s) 486, 897
MslI CAYNNNNRTG 1 cut(s) 1053
Msp20I TGGCCA 1 cut(s) 878
MspI CCGG 1 cut(s) 1053
MvnI CGCG 1 cut(s) 859
MwoI GCNNNNNNNGC 5 cut(s) 17, 453, 516, 884, 922
NdeII GATC 7 cut(s) 122, 269, 281, 315, 577, 963, 1291
NlaIII CATG 8 cut(s) 230, 298, 314, 625, 842, 875, 1052, 1314
NlaIV GGNNCC 2 cut(s) 183, 915
NsiI ATGCAT 2 cut(s) 1280, 1312
NspI RCATGY 4 cut(s) 298, 842, 875, 1052
NspV TTCGAA 1 cut(s) 772
PaeI GCATGC 1 cut(s) 298
PagI TCATGA 1 cut(s) 226
PaqCI CACCTGC 1 cut(s) 161
PciI ACATGT 2 cut(s) 838, 871
PciSI GCTCTTC 1 cut(s) 378
PcsI WCGNNNNNNNCGW 1 cut(s) 1127
PdmI GAANNNNTTC 2 cut(s) 363, 387
PfeI GAWTC 3 cut(s) 498, 552, 806
PkrI GCNGC 5 cut(s) 446, 509, 706, 942, 1098
PscI ACATGT 2 cut(s) 838, 871
PsiI TTATAA 1 cut(s) 648
Psp124BI GAGCTC 1 cut(s) 759
PspN4I GGNNCC 2 cut(s) 183, 915
PspPI GGNCC 1 cut(s) 914
PstI CTGCAG 2 cut(s) 446, 1101
PsuI RGATCY 2 cut(s) 315, 577
RsaI GTAC 3 cut(s) 752, 870, 1172
RsaNI GTAC 3 cut(s) 751, 869, 1171
RseI CAYNNNNRTG 1 cut(s) 1053
SacI GAGCTC 1 cut(s) 759
SapI GCTCTTC 1 cut(s) 378
SaqAI TTAA 2 cut(s) 486, 897
SatI GCNGC 5 cut(s) 445, 508, 705, 941, 1097
Sau3AI GATC 7 cut(s) 122, 269, 281, 315, 577, 963, 1291
Sau96I GGNCC 1 cut(s) 914
SduI GDGCHC 1 cut(s) 759
SfaNI GCATC 6 cut(s) 29, 285, 528, 772, 1332, 1339
SfcI CTRYAG 2 cut(s) 442, 1097
SfuI TTCGAA 1 cut(s) 772
SmiMI CAYNNNNRTG 1 cut(s) 1053
SmlI CTYRAG 1 cut(s) 1091
SmoI CTYRAG 1 cut(s) 1091
SphI GCATGC 1 cut(s) 298
SsiI CCGC 6 cut(s) 9, 178, 548, 859, 1064, 1283
SspMI CTAG 2 cut(s) 101, 345
SstI GAGCTC 1 cut(s) 759
StyI CCWWGG 1 cut(s) 255
TaaI ACNGT 6 cut(s) 85, 307, 376, 644, 855, 1262
TaiI ACGT 1 cut(s) 1133
TaqI TCGA 7 cut(s) 144, 201, 437, 560, 772, 946, 1121
TatI WGTACW 1 cut(s) 868
TfiI GAWTC 3 cut(s) 498, 552, 806
Tru1I TTAA 2 cut(s) 486, 897
Tru9I TTAA 2 cut(s) 486, 897
TscAI CASTG 5 cut(s) 100, 312, 609, 858, 1267
TseI GCWGC 5 cut(s) 444, 507, 704, 940, 1096
TspDTI ATGAA 6 cut(s) 187, 215, 321, 462, 843, 1143
TspRI CASTG 5 cut(s) 100, 312, 609, 858, 1267
XapI RAATTY 4 cut(s) 194, 222, 598, 1016
XceI RCATGY 4 cut(s) 298, 842, 875, 1052
XmiI GTMKAC 1 cut(s) 1045
XmnI GAANNNNTTC 2 cut(s) 363, 387
XspI CTAG 2 cut(s) 101, 345
Zsp2I ATGCAT 2 cut(s) 1280, 1312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.