RchiOBHm_Chr7g0222201

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0008224
Physical Location & Seq
Forward (+)
43370681 .. 43371568
888 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19885

Sequence Viewer

Length: 792 bp
ATGATCATAAACAAGTTTGGATTTCGAAGCAACATAAAGAGCGTCAACCTCAGTGGAATGGGCTGCAGTGCTGGAATCTTGTCAATTTATTTGGCCAAAGATCTTCTCAAAGTTCATAAAAACTCATTGGCATTAGTCCTCAGCATGGAAGCTGTAACTCCCAATGGCTATGCGGGTAAGAAAAAATCAATGCTTCTTCCAAACATCTTGTTTCGGATGGGAGGAGCTGCTATTCTACTATCGAATAGGAAGCAAGACAAGGGAATGGCAAAGTATAAGCTTGAGCATCTTGTTCAAACCCACATTGGTTCGGATGACGAAGCATATCAATCTGTTTTCCAGAAATCAGATGAAGATGGAGTTGAAGGGGTCTCACTATCAAGAGCCCTACTAAATGTGGCAACAAAAGCTCTAAAAACAAACATATCATCTCTGGGGCCACTTGTGCTACCACACTCCGAGCAGCTTCGGTTCGCATGGTCCATGATCCCAGTGATCGATGGCATAGAGCAAAGTCTGAAACTGCAGAAGGAAGATGGAGAGGCTTCAAGAATGACATTACATAGATTTGGAAACACATCATCTTCTTCAATCTGGTATGAGCTTTGCTATTTGGAAACAAAAGGAAGGGTGAAGAAAGGAAACAAAGTTTGGCAAATTGCATTTGGAAGTGGCTTCAAGTGTAACACTGCAGTTTGGAGATGCATTTCAGATGTTGATCCAACAAAAAGAAATGCATGGTCAGACAGCATCAATTTGTATCCTATTATTGATGATGAGATCCCAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

263

Amino Acids

29.1

Weight (kDa)

9.24

Isoelectric Point (pI)

36.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 1 - 163 2e-73 FAE1/Type III polyketide synthase-like protein
Chal_sti_synt_C PF02797 165 - 236 1.6e-06 Chalcone and stilbene synthases, C-terminal domain
ACP_syn_III_C PF08541 172 - 234 1.4e-09 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 173
AclWI GGATC 3 cut(s) 481, 713, 775
AcoI YGGCCR 1 cut(s) 93
AfiI CCNNNNNNNGG 1 cut(s) 145
AgsI TTSAA 5 cut(s) 296, 365, 549, 591, 679
AjuI GAANNNNNNNTTGG 2 cut(s) 634, 666
AluBI AGCT 6 cut(s) 152, 227, 280, 410, 466, 604
AluI AGCT 6 cut(s) 152, 227, 280, 410, 466, 604
Alw26I GTCTC 1 cut(s) 376
AlwI GGATC 3 cut(s) 481, 713, 775
AoxI GGCC 2 cut(s) 93, 437
ApeKI GCWGC 3 cut(s) 63, 227, 463
AspS9I GGNCC 2 cut(s) 437, 480
AsuHPI GGTGA 1 cut(s) 643
AsuII TTCGAA 1 cut(s) 25
AvaII GGWCC 1 cut(s) 480
BalI TGGCCA 1 cut(s) 95
BanII GRGCYC 1 cut(s) 388
BbvCI CCTCAGC 1 cut(s) 140
BbvI GCAGC 3 cut(s) 50, 214, 475
BccI CCATC 4 cut(s) 211, 350, 494, 530
BciVI GTATCC 1 cut(s) 771
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 1 cut(s) 376
BfmI CTRYAG 3 cut(s) 64, 524, 690
BfuI GTATCC 1 cut(s) 771
BglII AGATCT 1 cut(s) 100
BisI GCNGC 3 cut(s) 64, 228, 464
BlsI GCNGC 3 cut(s) 65, 229, 465
Bme18I GGWCC 1 cut(s) 480
BmgT120I GGNCC 2 cut(s) 437, 480
BmiI GGNNCC 1 cut(s) 438
BmrI ACTGGG 1 cut(s) 485
BmsI GCATC 3 cut(s) 295, 692, 759
BmuI ACTGGG 1 cut(s) 485
Bpu10I CCTNAGC 1 cut(s) 140
Bpu14I TTCGAA 1 cut(s) 25
BpuEI CTTGAG 1 cut(s) 302
Bsa29I ATCGAT 1 cut(s) 498
BsaBI GATNNNNATC 1 cut(s) 717
BsaI GGTCTC 1 cut(s) 376
Bsc4I CCNNNNNNNGG 1 cut(s) 145
Bse1I ACTGG 1 cut(s) 491
Bse8I GATNNNNATC 1 cut(s) 717
BseCI ATCGAT 1 cut(s) 498
BseGI GGATG 2 cut(s) 222, 319
BseJI GATNNNNATC 1 cut(s) 717
BseLI CCNNNNNNNGG 1 cut(s) 145
BseMII CTCAG 2 cut(s) 64, 154
BseNI ACTGG 1 cut(s) 491
BseRI GAGGAG 1 cut(s) 237
BseXI GCAGC 3 cut(s) 50, 214, 475
BshFI GGCC 2 cut(s) 95, 439
BshVI ATCGAT 1 cut(s) 498
BslI CCNNNNNNNGG 1 cut(s) 145
BsmAI GTCTC 1 cut(s) 376
BsnI GGCC 2 cut(s) 95, 439
Bso31I GGTCTC 1 cut(s) 376
Bsp119I TTCGAA 1 cut(s) 25
Bsp1286I GDGCHC 1 cut(s) 388
Bsp143I GATC 6 cut(s) 3, 100, 486, 495, 718, 780
BspACI CCGC 1 cut(s) 173
BspANI GGCC 2 cut(s) 95, 439
BspCNI CTCAG 2 cut(s) 63, 153
BspDI ATCGAT 1 cut(s) 498
BspLI GGNNCC 1 cut(s) 438
BspMAI CTGCAG 3 cut(s) 68, 528, 694
BspPI GGATC 3 cut(s) 481, 713, 775
BspT104I TTCGAA 1 cut(s) 25
BspTNI GGTCTC 1 cut(s) 376
BsrI ACTGG 1 cut(s) 491
BssMI GATC 6 cut(s) 3, 100, 486, 495, 718, 780
BstBI TTCGAA 1 cut(s) 25
BstDEI CTNAG 2 cut(s) 50, 140
BstF5I GGATG 2 cut(s) 222, 319
BstKTI GATC 6 cut(s) 6, 103, 489, 498, 721, 783
BstMAI GTCTC 1 cut(s) 376
BstMBI GATC 6 cut(s) 3, 100, 486, 495, 718, 780
BstMWI GCNNNNNNNGC 2 cut(s) 407, 445
BstSFI CTRYAG 3 cut(s) 64, 524, 690
BstV1I GCAGC 3 cut(s) 50, 214, 475
BstX2I RGATCY 2 cut(s) 100, 780
BstYI RGATCY 2 cut(s) 100, 780
Bsu15I ATCGAT 1 cut(s) 498
BsuI GTATCC 1 cut(s) 771
BsuRI GGCC 2 cut(s) 95, 439
BsuTUI ATCGAT 1 cut(s) 498
BtsCI GGATG 2 cut(s) 222, 319
BtsI GCAGTG 2 cut(s) 73, 687
BtsIMutI CAGTG 4 cut(s) 58, 73, 498, 687
Cfr13I GGNCC 2 cut(s) 437, 480
ClaI ATCGAT 1 cut(s) 498
CseI GACGC 1 cut(s) 31
CspCI CAANNNNNGTGG 2 cut(s) 34, 69
CviAII CATG 4 cut(s) 145, 477, 484, 738
DdeI CTNAG 2 cut(s) 50, 140
DpnI GATC 6 cut(s) 5, 102, 488, 497, 720, 782
DpnII GATC 6 cut(s) 3, 100, 486, 495, 718, 780
EaeI YGGCCR 1 cut(s) 93
Eco24I GRGCYC 1 cut(s) 388
Eco31I GGTCTC 1 cut(s) 376
Eco47I GGWCC 1 cut(s) 480
EcoT22I ATGCAT 2 cut(s) 707, 739
EcoT38I GRGCYC 1 cut(s) 388
FaeI CATG 4 cut(s) 148, 480, 487, 741
FatI CATG 4 cut(s) 144, 476, 483, 737
FauI CCCGC 1 cut(s) 166
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 3 cut(s) 64, 228, 464
FokI GGATG 2 cut(s) 229, 326
FriOI GRGCYC 1 cut(s) 388
Fsp4HI GCNGC 3 cut(s) 64, 228, 464
GluI GCNGC 3 cut(s) 64, 228, 464
HaeIII GGCC 2 cut(s) 95, 439
HgaI GACGC 1 cut(s) 31
Hin1II CATG 4 cut(s) 148, 480, 487, 741
HincII GTYRAC 1 cut(s) 46
HindII GTYRAC 1 cut(s) 46
HindIII AAGCTT 1 cut(s) 278
HinfI GANTC 1 cut(s) 75
HphI GGTGA 1 cut(s) 643
Hpy166II GTNNAC 1 cut(s) 46
Hpy188I TCNGA 7 cut(s) 216, 313, 349, 460, 519, 712, 745
Hpy188III TCNNGA 3 cut(s) 340, 381, 549
Hpy8I GTNNAC 1 cut(s) 46
HpyAV CCTTC 3 cut(s) 359, 523, 621
HpyCH4V TGCA 6 cut(s) 66, 526, 662, 692, 705, 737
HpyF10VI GCNNNNNNNGC 2 cut(s) 407, 445
HpyF3I CTNAG 2 cut(s) 50, 140
Hsp92II CATG 4 cut(s) 148, 480, 487, 741
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 6 cut(s) 3, 100, 486, 495, 718, 780
LmnI GCTCC 1 cut(s) 224
LpnPI CCDG 5 cut(s) 57, 353, 419, 504, 580
Lsp1109I GCAGC 3 cut(s) 50, 214, 475
LweI GCATC 3 cut(s) 295, 692, 759
MaeIII GTNAC 2 cut(s) 154, 683
MalI GATC 6 cut(s) 5, 102, 488, 497, 720, 782
MboI GATC 6 cut(s) 3, 100, 486, 495, 718, 780
MboII GAAGA 7 cut(s) 95, 188, 365, 545, 576, 579, 646
MflI RGATCY 2 cut(s) 100, 780
MhlI GDGCHC 1 cut(s) 388
MlsI TGGCCA 1 cut(s) 95
MluCI AATT 3 cut(s) 84, 657, 754
MluNI TGGCCA 1 cut(s) 95
MmeI TCCRAC 1 cut(s) 746
MnlI CCTC 4 cut(s) 59, 149, 215, 535
Mox20I TGGCCA 1 cut(s) 95
Mph1103I ATGCAT 2 cut(s) 707, 739
MscI TGGCCA 1 cut(s) 95
Msp20I TGGCCA 1 cut(s) 95
MwoI GCNNNNNNNGC 2 cut(s) 407, 445
NdeII GATC 6 cut(s) 3, 100, 486, 495, 718, 780
NlaIII CATG 4 cut(s) 148, 480, 487, 741
NlaIV GGNNCC 1 cut(s) 438
NsiI ATGCAT 2 cut(s) 707, 739
NspV TTCGAA 1 cut(s) 25
PfeI GAWTC 1 cut(s) 75
PkrI GCNGC 3 cut(s) 65, 229, 465
PspN4I GGNNCC 1 cut(s) 438
PspPI GGNCC 2 cut(s) 437, 480
PstI CTGCAG 3 cut(s) 68, 528, 694
PsuI RGATCY 2 cut(s) 100, 780
SatI GCNGC 3 cut(s) 64, 228, 464
Sau3AI GATC 6 cut(s) 3, 100, 486, 495, 718, 780
Sau96I GGNCC 2 cut(s) 437, 480
SduI GDGCHC 1 cut(s) 388
SetI ASST 7 cut(s) 51, 154, 229, 282, 412, 468, 606
SfaNI GCATC 3 cut(s) 295, 692, 759
SfcI CTRYAG 3 cut(s) 64, 524, 690
SfuI TTCGAA 1 cut(s) 25
SinI GGWCC 1 cut(s) 480
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
Sse9I AATT 3 cut(s) 84, 657, 754
SsiI CCGC 1 cut(s) 173
TaqI TCGA 3 cut(s) 25, 242, 498
TasI AATT 3 cut(s) 84, 657, 754
TfiI GAWTC 1 cut(s) 75
TscAI CASTG 4 cut(s) 58, 73, 498, 694
TseI GCWGC 3 cut(s) 63, 227, 463
TspDTI ATGAA 2 cut(s) 104, 366
TspRI CASTG 4 cut(s) 58, 73, 498, 694
VpaK11BI GGWCC 1 cut(s) 480
Zsp2I ATGCAT 2 cut(s) 707, 739
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.