FvH4_5g29660

plastid-lipid-associated protein 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0005370
Physical Location & Seq
Forward (+)
20592406 .. 20597400
4995 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g29660.t1

Sequence Viewer

Length: 726 bp
ATGAGTCTGAGTATGGCATTATCTTCTTCTTGGCCTCCGACACTCCTTACAACCACCCAATCCTCCTCAACTCCCAGACTAGTCCCACTCACCTCCTTGAGCTTCCCTTCCAAACCCACCACCACTCCCACCATTGTTCCCGCGCTCTCCCACAAATGGAGAGCCAGAATCTCATTCTTCCCTGCATTTCTGAGCAAAGGCAAAGACGCCAAAACTCTCAAGGAGGAGCTTCTCGATGCCATTGCTTCACTCGATCGAGGCGCCGATGCCACTCCGGAGGACCAGCAAGCTGTTGATCAGATTACACGCAAACTTGAAGCAGTTACTCCAGTAAAGGAACCACTCAAATCTGATCTACTGGATGGAAAATGGGAGCTTCTCTACACTACTTCCAAATCTATTCTCCAAACTGGGAGACCCAAGTTTCTAAGATCGAGGGTAAATTACCAAGCCATCAATGTGGACACACTCAGGGCCCAAAATATGGAGTCATGGCCAACTTTCAATCAGGTAACAGCTGATTTAACACCTTTGAATGCAAGAAAAGTGGCTGTAAAGTTCGACTATTTCAAGATTGCAGGCCTGATACCTGTTAAAGCACCTGACAGAGCCCGTGGTGAACTGGAAATTACATATTTGGATGAAGAATTGCGTGTCTCGAGAGGTGATAAAGGAAACCTGTTCATCTTGAAAATGGTTGATCCATCTTATCGAGTTCCTGTCTGA

Protein Analysis

242

Amino Acids

26.88

Weight (kDa)

9.54

Isoelectric Point (pI)

33.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP_fibrillin PF04755 72 - 231 4.3e-37 PAP_fibrillin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 260
AccB7I CCANNNNNTGG 1 cut(s) 484
AccII CGCG 1 cut(s) 143
AccIII TCCGGA 1 cut(s) 274
AciI CCGC 1 cut(s) 141
AclWI GGATC 1 cut(s) 695
AcoI YGGCCR 1 cut(s) 494
AcyI GRCGYC 2 cut(s) 207, 261
AfiI CCNNNNNNNGG 2 cut(s) 156, 484
AgsI TTSAA 5 cut(s) 317, 505, 535, 571, 691
AhlI ACTAGT 1 cut(s) 79
AluBI AGCT 5 cut(s) 102, 229, 290, 376, 518
AluI AGCT 5 cut(s) 102, 229, 290, 376, 518
Alw26I GTCTC 2 cut(s) 409, 661
AlwI GGATC 1 cut(s) 695
Ama87I CYCGRG 1 cut(s) 658
Aor13HI TCCGGA 1 cut(s) 274
AoxI GGCC 4 cut(s) 32, 474, 494, 580
ApaI GGGCCC 1 cut(s) 478
AspLEI GCGC 2 cut(s) 145, 263
AspS9I GGNCC 3 cut(s) 280, 474, 475
AsuHPI GGTGA 3 cut(s) 82, 629, 677
AvaI CYCGRG 1 cut(s) 658
AvaII GGWCC 1 cut(s) 280
BaeGI GKGCMC 1 cut(s) 478
BalI TGGCCA 1 cut(s) 496
BanI GGYRCC 1 cut(s) 260
BanII GRGCYC 2 cut(s) 478, 613
BccI CCATC 3 cut(s) 356, 461, 712
BcgI CGANNNNNNTGC 2 cut(s) 224, 258
BclI TGATCA 1 cut(s) 295
BcoDI GTCTC 2 cut(s) 409, 661
BcuI ACTAGT 1 cut(s) 79
BfaI CTAG 1 cut(s) 80
BfoI RGCGCY 1 cut(s) 264
Bme18I GGWCC 1 cut(s) 280
BmeT110I CYCGRG 1 cut(s) 658
BmgT120I GGNCC 3 cut(s) 280, 474, 475
BmiI GGNNCC 3 cut(s) 262, 339, 476
BmrI ACTGGG 1 cut(s) 420
BmsI GCATC 2 cut(s) 226, 256
BmuI ACTGGG 1 cut(s) 420
BpmI CTGGAG 1 cut(s) 312
BpuEI CTTGAG 2 cut(s) 118, 203
BsaHI GRCGYC 2 cut(s) 207, 261
BsaI GGTCTC 1 cut(s) 409
BsaJI CCNNGG 1 cut(s) 613
BsaWI WCCGGW 1 cut(s) 274
BsaXI ACNNNNNCTCC 2 cut(s) 109, 139
Bsc4I CCNNNNNNNGG 2 cut(s) 156, 484
Bse1I ACTGG 4 cut(s) 329, 363, 415, 627
Bse3DI GCAATG 1 cut(s) 240
BseAI TCCGGA 1 cut(s) 274
BseDI CCNNGG 1 cut(s) 613
BseGI GGATG 2 cut(s) 367, 646
BseLI CCNNNNNNNGG 2 cut(s) 156, 484
BseMI GCAATG 1 cut(s) 240
BseMII CTCAG 2 cut(s) 182, 484
BseNI ACTGG 4 cut(s) 329, 363, 415, 627
BseRI GAGGAG 2 cut(s) 55, 239
BseSI GKGCMC 1 cut(s) 478
Bsh1236I CGCG 1 cut(s) 143
Bsh1285I CGRYCG 1 cut(s) 256
BshFI GGCC 4 cut(s) 34, 476, 496, 582
BshNI GGYRCC 1 cut(s) 260
BsiEI CGRYCG 1 cut(s) 256
BsiHKCI CYCGRG 1 cut(s) 658
BsiSI CCGG 1 cut(s) 275
BslFI GGGAC 1 cut(s) 68
BslI CCNNNNNNNGG 2 cut(s) 156, 484
BsmAI GTCTC 2 cut(s) 409, 661
BsmFI GGGAC 1 cut(s) 68
BsmI GAATGC 1 cut(s) 541
BsnI GGCC 4 cut(s) 34, 476, 496, 582
Bso31I GGTCTC 1 cut(s) 409
BsoBI CYCGRG 1 cut(s) 658
Bsp120I GGGCCC 1 cut(s) 474
Bsp1286I GDGCHC 2 cut(s) 478, 613
Bsp13I TCCGGA 1 cut(s) 274
Bsp143I GATC 5 cut(s) 253, 295, 352, 431, 700
BspACI CCGC 1 cut(s) 141
BspANI GGCC 4 cut(s) 34, 476, 496, 582
BspCNI CTCAG 2 cut(s) 183, 483
BspEI TCCGGA 1 cut(s) 274
BspFNI CGCG 1 cut(s) 143
BspLI GGNNCC 3 cut(s) 262, 339, 476
BspPI GGATC 1 cut(s) 695
BspT107I GGYRCC 1 cut(s) 260
BspTNI GGTCTC 1 cut(s) 409
BsrDI GCAATG 1 cut(s) 240
BsrI ACTGG 4 cut(s) 329, 363, 415, 627
BssECI CCNNGG 1 cut(s) 613
BssMI GATC 5 cut(s) 253, 295, 352, 431, 700
BssNI GRCGYC 2 cut(s) 207, 261
BstACI GRCGYC 2 cut(s) 207, 261
BstC8I GCNNGC 2 cut(s) 288, 580
BstDEI CTNAG 4 cut(s) 8, 191, 428, 470
BstDSI CCRYGG 1 cut(s) 613
BstF5I GGATG 2 cut(s) 367, 646
BstFNI CGCG 1 cut(s) 143
BstH2I RGCGCY 1 cut(s) 264
BstHHI GCGC 2 cut(s) 145, 263
BstKTI GATC 5 cut(s) 256, 298, 355, 434, 703
BstMAI GTCTC 2 cut(s) 409, 661
BstMBI GATC 5 cut(s) 253, 295, 352, 431, 700
BstMCI CGRYCG 1 cut(s) 256
BstSLI GKGCMC 1 cut(s) 478
BstUI CGCG 1 cut(s) 143
BstXI CCANNNNNNTGG 1 cut(s) 460
BsuRI GGCC 4 cut(s) 34, 476, 496, 582
BtgI CCRYGG 1 cut(s) 613
BtsCI GGATG 2 cut(s) 367, 646
Cac8I GCNNGC 2 cut(s) 288, 580
CfoI GCGC 2 cut(s) 145, 263
Cfr13I GGNCC 3 cut(s) 280, 474, 475
CseI GACGC 1 cut(s) 215
CspCI CAANNNNNGTGG 2 cut(s) 528, 563
CviAII CATG 1 cut(s) 492
DdeI CTNAG 4 cut(s) 8, 191, 428, 470
DinI GGCGCC 1 cut(s) 262
DpnI GATC 5 cut(s) 255, 297, 354, 433, 702
DpnII GATC 5 cut(s) 253, 295, 352, 431, 700
EaeI YGGCCR 1 cut(s) 494
Eco147I AGGCCT 1 cut(s) 582
Eco24I GRGCYC 2 cut(s) 478, 613
Eco31I GGTCTC 1 cut(s) 409
Eco47I GGWCC 1 cut(s) 280
Eco88I CYCGRG 1 cut(s) 658
EcoO109I RGGNCCY 1 cut(s) 474
EcoT38I GRGCYC 2 cut(s) 478, 613
EgeI GGCGCC 1 cut(s) 262
EheI GGCGCC 1 cut(s) 262
FaeI CATG 1 cut(s) 495
FaiI YATR 4 cut(s) 14, 485, 493, 634
FaqI GGGAC 1 cut(s) 68
FatI CATG 1 cut(s) 491
FauI CCCGC 1 cut(s) 148
FbaI TGATCA 1 cut(s) 295
FokI GGATG 2 cut(s) 374, 653
FriOI GRGCYC 2 cut(s) 478, 613
FspBI CTAG 1 cut(s) 80
GlaI GCGC 2 cut(s) 144, 262
GsuI CTGGAG 1 cut(s) 312
HaeII RGCGCY 1 cut(s) 264
HaeIII GGCC 4 cut(s) 34, 476, 496, 582
HapII CCGG 1 cut(s) 275
HgaI GACGC 1 cut(s) 215
HhaI GCGC 2 cut(s) 145, 263
Hin1I GRCGYC 2 cut(s) 207, 261
Hin1II CATG 1 cut(s) 495
Hin6I GCGC 2 cut(s) 143, 261
HinP1I GCGC 2 cut(s) 143, 261
HinfI GANTC 3 cut(s) 4, 168, 488
HpaII CCGG 1 cut(s) 275
HphI GGTGA 3 cut(s) 82, 629, 677
Hpy166II GTNNAC 2 cut(s) 463, 620
Hpy188I TCNGA 6 cut(s) 9, 39, 192, 300, 352, 725
Hpy188III TCNNGA 6 cut(s) 233, 275, 571, 658, 660, 688
Hpy8I GTNNAC 2 cut(s) 463, 620
HpyAV CCTTC 1 cut(s) 117
HpyCH4V TGCA 3 cut(s) 185, 539, 578
HpyF3I CTNAG 4 cut(s) 8, 191, 428, 470
Hsp92I GRCGYC 2 cut(s) 207, 261
Hsp92II CATG 1 cut(s) 495
HspAI GCGC 2 cut(s) 143, 261
KasI GGCGCC 1 cut(s) 260
Kpn2I TCCGGA 1 cut(s) 274
Ksp22I TGATCA 1 cut(s) 295
Kzo9I GATC 5 cut(s) 253, 295, 352, 431, 700
LmnI GCTCC 2 cut(s) 226, 373
LweI GCATC 2 cut(s) 226, 256
MaeI CTAG 1 cut(s) 80
MaeIII GTNAC 2 cut(s) 322, 511
MalI GATC 5 cut(s) 255, 297, 354, 433, 702
MboI GATC 5 cut(s) 253, 295, 352, 431, 700
MboII GAAGA 4 cut(s) 15, 18, 169, 656
MhlI GDGCHC 2 cut(s) 478, 613
MlsI TGGCCA 1 cut(s) 496
MluCI AATT 3 cut(s) 442, 627, 647
MluNI TGGCCA 1 cut(s) 496
Mly113I GGCGCC 1 cut(s) 261
MlyI GAGTC 2 cut(s) 13, 497
MmeI TCCRAC 1 cut(s) 62
MnlI CCTC 9 cut(s) 45, 73, 76, 103, 217, 251, 271, 429, 656
Mox20I TGGCCA 1 cut(s) 496
MroI TCCGGA 1 cut(s) 274
MscI TGGCCA 1 cut(s) 496
MseI TTAA 2 cut(s) 524, 594
MslI CAYNNNNRTG 1 cut(s) 458
Msp20I TGGCCA 1 cut(s) 496
MspA1I CMGCKG 1 cut(s) 518
MspI CCGG 1 cut(s) 275
Mva1269I GAATGC 1 cut(s) 541
MvnI CGCG 1 cut(s) 143
NarI GGCGCC 1 cut(s) 261
NdeII GATC 5 cut(s) 253, 295, 352, 431, 700
NlaIII CATG 1 cut(s) 495
NlaIV GGNNCC 3 cut(s) 262, 339, 476
PaeR7I CTCGAG 1 cut(s) 658
PceI AGGCCT 1 cut(s) 582
PctI GAATGC 1 cut(s) 541
PfeI GAWTC 1 cut(s) 168
PflMI CCANNNNNTGG 1 cut(s) 484
Ple19I CGATCG 1 cut(s) 256
PleI GAGTC 2 cut(s) 12, 496
PluTI GGCGCC 1 cut(s) 264
PpsI GAGTC 2 cut(s) 12, 496
PspN4I GGNNCC 3 cut(s) 262, 339, 476
PspOMI GGGCCC 1 cut(s) 474
PspPI GGNCC 3 cut(s) 280, 474, 475
PvuI CGATCG 1 cut(s) 256
PvuII CAGCTG 1 cut(s) 518
RseI CAYNNNNRTG 1 cut(s) 458
SaqAI TTAA 2 cut(s) 524, 594
Sau3AI GATC 5 cut(s) 253, 295, 352, 431, 700
Sau96I GGNCC 3 cut(s) 280, 474, 475
SchI GAGTC 2 cut(s) 13, 497
SduI GDGCHC 2 cut(s) 478, 613
SfaNI GCATC 2 cut(s) 226, 256
SfoI GGCGCC 1 cut(s) 262
Sfr274I CTCGAG 1 cut(s) 658
SinI GGWCC 1 cut(s) 280
SlaI CTCGAG 1 cut(s) 658
SmiMI CAYNNNNRTG 1 cut(s) 458
SmlI CTYRAG 3 cut(s) 97, 218, 658
SmoI CTYRAG 3 cut(s) 97, 218, 658
SpeI ACTAGT 1 cut(s) 79
Sse9I AATT 3 cut(s) 442, 627, 647
SseBI AGGCCT 1 cut(s) 582
SsiI CCGC 1 cut(s) 141
SspDI GGCGCC 1 cut(s) 260
SspMI CTAG 1 cut(s) 80
StuI AGGCCT 1 cut(s) 582
TaqI TCGA 7 cut(s) 234, 252, 256, 434, 561, 659, 712
TasI AATT 3 cut(s) 442, 627, 647
TfiI GAWTC 1 cut(s) 168
Tru1I TTAA 2 cut(s) 524, 594
Tru9I TTAA 2 cut(s) 524, 594
TspDTI ATGAA 2 cut(s) 657, 673
Van91I CCANNNNNTGG 1 cut(s) 484
VpaK11BI GGWCC 1 cut(s) 280
XhoI CTCGAG 1 cut(s) 658
XspI CTAG 1 cut(s) 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.