Rw7G030380

plastid-lipid-associated protein 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0005370
Physical Location & Seq
Forward (+)
43101204 .. 43104788
3585 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G030380.1

Sequence Viewer

Length: 759 bp
ATGGCATTATCTTCTTCTTGGTCACCTACACTCCTTACAACCACCCAATCCTCCTCACCTCCCAAACTAGTCCATTCTCATTCCCCGCTCACCTCCTTGACCTTCCCTTCCAAACCCACCACCACCACCCACACCATTGTTCCCGCGCTTTCCCACAAATGGAGAGCCAGAATTTCCTTCTTTCCTGCTTTTTCGAGCAAAGGCAAAGACGCCAAAACTCTCAAGGAGGAGCTTCTCGATGCCATTGCTTCGCTCGATCGAGGCGCCGACGCCACTCCAGAGGACCAGCAAGCTGTTGATCAGATTGCACGCAAACTTGAAGCAGTCACTCCAGTAAAGGAGACACTCAAATCTGGTCTACTCGACGGAAAATGGGAGCTTCTCTACACTACTTCAAAATCACTTCTCCAAACTGGGAGACCCAAGTTTTTAAGATCGAGAGTGAACTACCAAGCAATCAATGTGGACACTCTGAGGGCCCAAAATATGGAGTCGTGGCCAACTTTCAATCAGGTAACAGCCGATTTAATACCTCTGAATGCAAGAAAAGTGGCAGTAAAATTTGACTATTTCAAGATTGCAGGCCTGATACCTGTTAAGGCACCAGACAAAACCCCTGGTGAACTGGAAATTACATATTTGGATGAAGAGTTGCTTAAGATTGACTTATGCCACCATGATATACAGATACCTGTTAAGGCACCAGACAAAACCCCTGGTGAACTGGAAATTACATATTTGGATGAAGAGTTGCGGTGA

Protein Analysis

252

Amino Acids

28.06

Weight (kDa)

7.03

Isoelectric Point (pI)

35.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP_fibrillin PF04755 73 - 252 5.2e-30 PAP_fibrillin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 263, 601, 700
AccB7I CCANNNNNTGG 1 cut(s) 487
AccBSI CCGCTC 1 cut(s) 88
AccI GTMKAC 1 cut(s) 358
AccII CGCG 1 cut(s) 146
AciI CCGC 3 cut(s) 86, 144, 754
AcoI YGGCCR 1 cut(s) 497
AcsI RAATTY 2 cut(s) 171, 560
AcyI GRCGYC 3 cut(s) 210, 264, 270
AfiI CCNNNNNNNGG 2 cut(s) 159, 487
AflII CTTAAG 1 cut(s) 656
AgsI TTSAA 4 cut(s) 320, 396, 508, 574
AhlI ACTAGT 1 cut(s) 67
AjnI CCWGG 2 cut(s) 616, 715
AluBI AGCT 3 cut(s) 232, 293, 379
AluI AGCT 3 cut(s) 232, 293, 379
Alw26I GTCTC 2 cut(s) 335, 412
AoxI GGCC 3 cut(s) 477, 497, 583
ApaI GGGCCC 1 cut(s) 481
ApoI RAATTY 2 cut(s) 171, 560
AspLEI GCGC 2 cut(s) 148, 266
AspS9I GGNCC 3 cut(s) 283, 477, 478
AsuHPI GGTGA 5 cut(s) 15, 48, 82, 632, 731
AvaII GGWCC 1 cut(s) 283
BaeGI GKGCMC 1 cut(s) 481
BalI TGGCCA 1 cut(s) 499
BanI GGYRCC 3 cut(s) 263, 601, 700
BanII GRGCYC 1 cut(s) 481
BcgI CGANNNNNNTGC 2 cut(s) 227, 261
BciT130I CCWGG 2 cut(s) 618, 717
BclI TGATCA 1 cut(s) 298
BcoDI GTCTC 2 cut(s) 335, 412
BcuI ACTAGT 1 cut(s) 67
BfaI CTAG 1 cut(s) 68
BfoI RGCGCY 1 cut(s) 267
BfrI CTTAAG 1 cut(s) 656
Bme1390I CCNGG 2 cut(s) 618, 717
Bme18I GGWCC 1 cut(s) 283
BmgT120I GGNCC 3 cut(s) 283, 477, 478
BmiI GGNNCC 4 cut(s) 265, 479, 603, 702
BmrFI CCNGG 2 cut(s) 618, 717
BmrI ACTGGG 1 cut(s) 423
BmsI GCATC 1 cut(s) 229
BmuI ACTGGG 1 cut(s) 423
BpmI CTGGAG 2 cut(s) 261, 315
BpuEI CTTGAG 1 cut(s) 206
BsaHI GRCGYC 3 cut(s) 210, 264, 270
BsaI GGTCTC 1 cut(s) 412
BsaJI CCNNGG 2 cut(s) 616, 715
BsaXI ACNNNNNCTCC 2 cut(s) 15, 45
Bsc4I CCNNNNNNNGG 2 cut(s) 159, 487
Bse1I ACTGG 4 cut(s) 332, 418, 630, 729
Bse3DI GCAATG 1 cut(s) 243
BseBI CCWGG 2 cut(s) 618, 717
BseDI CCNNGG 2 cut(s) 616, 715
BseGI GGATG 2 cut(s) 649, 748
BseLI CCNNNNNNNGG 2 cut(s) 159, 487
BseMI GCAATG 1 cut(s) 243
BseMII CTCAG 1 cut(s) 464
BseNI ACTGG 4 cut(s) 332, 418, 630, 729
BseRI GAGGAG 2 cut(s) 43, 242
BseSI GKGCMC 1 cut(s) 481
Bsh1236I CGCG 1 cut(s) 146
Bsh1285I CGRYCG 1 cut(s) 259
BshFI GGCC 3 cut(s) 479, 499, 585
BshNI GGYRCC 3 cut(s) 263, 601, 700
BsiEI CGRYCG 1 cut(s) 259
BslI CCNNNNNNNGG 2 cut(s) 159, 487
BsmAI GTCTC 2 cut(s) 335, 412
BsmI GAATGC 1 cut(s) 544
BsnI GGCC 3 cut(s) 479, 499, 585
Bso31I GGTCTC 1 cut(s) 412
Bsp120I GGGCCC 1 cut(s) 477
Bsp1286I GDGCHC 1 cut(s) 481
Bsp143I GATC 3 cut(s) 256, 298, 434
BspACI CCGC 3 cut(s) 86, 144, 754
BspANI GGCC 3 cut(s) 479, 499, 585
BspCNI CTCAG 1 cut(s) 465
BspFNI CGCG 1 cut(s) 146
BspLI GGNNCC 4 cut(s) 265, 479, 603, 702
BspT107I GGYRCC 3 cut(s) 263, 601, 700
BspTI CTTAAG 1 cut(s) 656
BspTNI GGTCTC 1 cut(s) 412
BsrBI CCGCTC 1 cut(s) 88
BsrDI GCAATG 1 cut(s) 243
BsrI ACTGG 4 cut(s) 332, 418, 630, 729
BssECI CCNNGG 2 cut(s) 616, 715
BssMI GATC 3 cut(s) 256, 298, 434
BssNI GRCGYC 3 cut(s) 210, 264, 270
Bst2UI CCWGG 2 cut(s) 618, 717
Bst6I CTCTTC 2 cut(s) 642, 741
BstACI GRCGYC 3 cut(s) 210, 264, 270
BstAFI CTTAAG 1 cut(s) 656
BstC8I GCNNGC 3 cut(s) 291, 310, 583
BstDEI CTNAG 1 cut(s) 473
BstEII GGTNACC 1 cut(s) 21
BstF5I GGATG 2 cut(s) 649, 748
BstFNI CGCG 1 cut(s) 146
BstH2I RGCGCY 1 cut(s) 267
BstHHI GCGC 2 cut(s) 148, 266
BstKTI GATC 3 cut(s) 259, 301, 437
BstMAI GTCTC 2 cut(s) 335, 412
BstMBI GATC 3 cut(s) 256, 298, 434
BstMCI CGRYCG 1 cut(s) 259
BstNI CCWGG 2 cut(s) 618, 717
BstPI GGTNACC 1 cut(s) 21
BstSCI CCNGG 2 cut(s) 616, 715
BstSLI GKGCMC 1 cut(s) 481
BstUI CGCG 1 cut(s) 146
BsuRI GGCC 3 cut(s) 479, 499, 585
BtsCI GGATG 2 cut(s) 649, 748
Cac8I GCNNGC 3 cut(s) 291, 310, 583
CfoI GCGC 2 cut(s) 148, 266
Cfr13I GGNCC 3 cut(s) 283, 477, 478
CseI GACGC 2 cut(s) 218, 278
CspCI CAANNNNNGTGG 4 cut(s) 444, 479, 531, 566
CviAII CATG 1 cut(s) 677
CviJI RGCY 8 cut(s) 167, 232, 293, 379, 479, 499, 521, 585
CviKI_1 RGCY 8 cut(s) 167, 232, 293, 379, 479, 499, 521, 585
DdeI CTNAG 1 cut(s) 473
DinI GGCGCC 1 cut(s) 265
DpnI GATC 3 cut(s) 258, 300, 436
DpnII GATC 3 cut(s) 256, 298, 434
EaeI YGGCCR 1 cut(s) 497
Eam1104I CTCTTC 2 cut(s) 642, 741
EarI CTCTTC 2 cut(s) 642, 741
Eco147I AGGCCT 1 cut(s) 585
Eco24I GRGCYC 1 cut(s) 481
Eco31I GGTCTC 1 cut(s) 412
Eco47I GGWCC 1 cut(s) 283
Eco91I GGTNACC 1 cut(s) 21
EcoO109I RGGNCCY 1 cut(s) 477
EcoO65I GGTNACC 1 cut(s) 21
EcoRII CCWGG 2 cut(s) 616, 715
EcoT38I GRGCYC 1 cut(s) 481
EgeI GGCGCC 1 cut(s) 265
EheI GGCGCC 1 cut(s) 265
FaeI CATG 1 cut(s) 680
FaiI YATR 6 cut(s) 488, 637, 670, 678, 683, 736
FalI AAGNNNNNCTT 4 cut(s) 639, 671, 650, 682
FatI CATG 1 cut(s) 676
FauI CCCGC 2 cut(s) 93, 151
FbaI TGATCA 1 cut(s) 298
FblI GTMKAC 1 cut(s) 358
FokI GGATG 2 cut(s) 656, 755
FriOI GRGCYC 1 cut(s) 481
FspBI CTAG 1 cut(s) 68
GlaI GCGC 2 cut(s) 147, 265
GsuI CTGGAG 2 cut(s) 261, 315
HaeII RGCGCY 1 cut(s) 267
HaeIII GGCC 3 cut(s) 479, 499, 585
HgaI GACGC 2 cut(s) 218, 278
HhaI GCGC 2 cut(s) 148, 266
Hin1I GRCGYC 3 cut(s) 210, 264, 270
Hin1II CATG 1 cut(s) 680
Hin6I GCGC 2 cut(s) 146, 264
HinP1I GCGC 2 cut(s) 146, 264
HinfI GANTC 1 cut(s) 491
HphI GGTGA 5 cut(s) 15, 48, 82, 632, 731
Hpy166II GTNNAC 5 cut(s) 359, 445, 466, 623, 722
Hpy188I TCNGA 3 cut(s) 303, 474, 537
Hpy188III TCNNGA 4 cut(s) 236, 278, 438, 574
Hpy8I GTNNAC 5 cut(s) 359, 445, 466, 623, 722
Hpy99I CGWCG 2 cut(s) 272, 368
HpyAV CCTTC 3 cut(s) 112, 117, 187
HpyCH4V TGCA 3 cut(s) 308, 542, 581
HpyF3I CTNAG 1 cut(s) 473
Hsp92I GRCGYC 3 cut(s) 210, 264, 270
Hsp92II CATG 1 cut(s) 680
HspAI GCGC 2 cut(s) 146, 264
KasI GGCGCC 1 cut(s) 263
Ksp22I TGATCA 1 cut(s) 298
Kzo9I GATC 3 cut(s) 256, 298, 434
LmnI GCTCC 2 cut(s) 229, 376
LweI GCATC 1 cut(s) 229
MaeI CTAG 1 cut(s) 68
MaeIII GTNAC 3 cut(s) 21, 325, 514
MalI GATC 3 cut(s) 258, 300, 436
MbiI CCGCTC 1 cut(s) 88
MboI GATC 3 cut(s) 256, 298, 434
MboII GAAGA 4 cut(s) 3, 6, 659, 758
MhlI GDGCHC 1 cut(s) 481
MlsI TGGCCA 1 cut(s) 499
MluCI AATT 4 cut(s) 171, 560, 630, 729
MluNI TGGCCA 1 cut(s) 499
Mly113I GGCGCC 1 cut(s) 264
MlyI GAGTC 1 cut(s) 500
MnlI CCTC 9 cut(s) 61, 64, 69, 103, 220, 254, 274, 468, 543
Mox20I TGGCCA 1 cut(s) 499
MscI TGGCCA 1 cut(s) 499
MseI TTAA 5 cut(s) 431, 527, 597, 657, 696
Msp20I TGGCCA 1 cut(s) 499
MspCI CTTAAG 1 cut(s) 656
MspR9I CCNGG 2 cut(s) 618, 717
Mva1269I GAATGC 1 cut(s) 544
MvaI CCWGG 2 cut(s) 618, 717
MvnI CGCG 1 cut(s) 146
NarI GGCGCC 1 cut(s) 264
NdeII GATC 3 cut(s) 256, 298, 434
NlaIII CATG 1 cut(s) 680
NlaIV GGNNCC 4 cut(s) 265, 479, 603, 702
NmuCI GTSAC 2 cut(s) 21, 325
PceI AGGCCT 1 cut(s) 585
PctI GAATGC 1 cut(s) 544
PflMI CCANNNNNTGG 1 cut(s) 487
Ple19I CGATCG 1 cut(s) 259
PleI GAGTC 1 cut(s) 499
PluTI GGCGCC 1 cut(s) 267
PpsI GAGTC 1 cut(s) 499
Psp6I CCWGG 2 cut(s) 616, 715
PspEI GGTNACC 1 cut(s) 21
PspGI CCWGG 2 cut(s) 616, 715
PspN4I GGNNCC 4 cut(s) 265, 479, 603, 702
PspOMI GGGCCC 1 cut(s) 477
PspPI GGNCC 3 cut(s) 283, 477, 478
PvuI CGATCG 1 cut(s) 259
SaqAI TTAA 5 cut(s) 431, 527, 597, 657, 696
Sau3AI GATC 3 cut(s) 256, 298, 434
Sau96I GGNCC 3 cut(s) 283, 477, 478
SchI GAGTC 1 cut(s) 500
ScrFI CCNGG 2 cut(s) 618, 717
SduI GDGCHC 1 cut(s) 481
SfaNI GCATC 1 cut(s) 229
SfoI GGCGCC 1 cut(s) 265
SinI GGWCC 1 cut(s) 283
SmlI CTYRAG 2 cut(s) 221, 656
SmoI CTYRAG 2 cut(s) 221, 656
SpeI ACTAGT 1 cut(s) 67
Sse9I AATT 4 cut(s) 171, 560, 630, 729
SseBI AGGCCT 1 cut(s) 585
SsiI CCGC 3 cut(s) 86, 144, 754
SspDI GGCGCC 1 cut(s) 263
SspMI CTAG 1 cut(s) 68
StuI AGGCCT 1 cut(s) 585
StyD4I CCNGG 2 cut(s) 616, 715
TaqI TCGA 6 cut(s) 194, 237, 255, 259, 363, 437
TasI AATT 4 cut(s) 171, 560, 630, 729
Tru1I TTAA 5 cut(s) 431, 527, 597, 657, 696
Tru9I TTAA 5 cut(s) 431, 527, 597, 657, 696
TseFI GTSAC 2 cut(s) 21, 325
Tsp45I GTSAC 2 cut(s) 21, 325
TspDTI ATGAA 2 cut(s) 660, 759
TspGWI ACGGA 1 cut(s) 381
Van91I CCANNNNNTGG 1 cut(s) 487
Vha464I CTTAAG 1 cut(s) 656
VpaK11BI GGWCC 1 cut(s) 283
XapI RAATTY 2 cut(s) 171, 560
XmiI GTMKAC 1 cut(s) 358
XspI CTAG 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.