Rh7DG352000

plastid-lipid-associated protein 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0005370
Physical Location & Seq
Forward (+)
46856273 .. 46860166
3894 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG352000.1

Sequence Viewer

Length: 729 bp
ATGGCATTATCTTCTTCTTGGTCACCTACGCTCCTTACAACCACCCAATCCTCCTCACCTCCCAAACTAGTCCATTCTCATTCCCCGCTCACCTCCTTGAACTTCCCTTCCAAACCCACCACCACCACCCACACCATTGTTCCCGCGCTTTCCCACAAATGGAGAGCCAGAATTTCCTTCTTTCCTGCTTTTTCGAGCAAAGGCAAAGACGCCAAAACTCTCAAGGAGGAGCTTCTCGATGCCATTGCTTCGCTCGATCGAGGCGCCGACGCCACTCCAGAGGACCAGCAAGCTGTTGATCAGATTGCACGCAAACTTGAAGCAGTTACTCCAGTAAAGGAGACACTCAAATCTGGTCTACTCGACGGAAAATGGGAGCTTCTCTACACTACTTCAAAATCGCTTCTCCAAACTGGGAGACCCAAGTTTTTAAGATCGAGAGTGAACTACCAAGCAATCAATGTGGACACTCTCAGGGCCCAAAATATGGAGTCGTGGCCAACTTTCAATCAGGTAACAGCCGATTTAATACCTCTGAATGCAAGAACAGTGGCTGTAAAATTTGACTATTTCAAGATTGCAGGCCTGATACCTGTTAAGGCACCAGACAAAACCCCTGGTGAACTGGAAATTACATATTTGGATGAAGAGTTGCGTGTCTCAAGAGGTGATAAAGGAAACCTGTTCATCTTGAAATTGGTTGATCCATCCTATCGAGTTCCTGTCTGA

Protein Analysis

242

Amino Acids

26.83

Weight (kDa)

9.42

Isoelectric Point (pI)

33.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP_fibrillin PF04755 73 - 232 1.6e-34 PAP_fibrillin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 263, 601
AccB7I CCANNNNNTGG 1 cut(s) 487
AccBSI CCGCTC 1 cut(s) 88
AccI GTMKAC 1 cut(s) 358
AccII CGCG 1 cut(s) 146
AciI CCGC 2 cut(s) 86, 144
AclWI GGATC 1 cut(s) 698
AcoI YGGCCR 1 cut(s) 497
AcsI RAATTY 2 cut(s) 171, 560
AcyI GRCGYC 3 cut(s) 210, 264, 270
AfiI CCNNNNNNNGG 2 cut(s) 159, 487
AgsI TTSAA 6 cut(s) 100, 320, 396, 508, 574, 694
AhlI ACTAGT 1 cut(s) 67
AjnI CCWGG 1 cut(s) 616
AloI GAACNNNNNNTCC 2 cut(s) 92, 124
AluBI AGCT 3 cut(s) 232, 293, 379
AluI AGCT 3 cut(s) 232, 293, 379
Alw26I GTCTC 3 cut(s) 335, 412, 664
AlwI GGATC 1 cut(s) 698
AlwNI CAGNNNCTG 1 cut(s) 554
AoxI GGCC 3 cut(s) 477, 497, 583
ApaI GGGCCC 1 cut(s) 481
ApoI RAATTY 2 cut(s) 171, 560
AspLEI GCGC 2 cut(s) 148, 266
AspS9I GGNCC 3 cut(s) 283, 477, 478
AsuHPI GGTGA 5 cut(s) 15, 48, 82, 632, 680
AvaII GGWCC 1 cut(s) 283
BaeGI GKGCMC 1 cut(s) 481
BalI TGGCCA 1 cut(s) 499
BanI GGYRCC 2 cut(s) 263, 601
BanII GRGCYC 1 cut(s) 481
BccI CCATC 1 cut(s) 715
BcgI CGANNNNNNTGC 2 cut(s) 227, 261
BciT130I CCWGG 1 cut(s) 618
BclI TGATCA 1 cut(s) 298
BcoDI GTCTC 3 cut(s) 335, 412, 664
BcuI ACTAGT 1 cut(s) 67
BfaI CTAG 1 cut(s) 68
BfoI RGCGCY 1 cut(s) 267
Bme1390I CCNGG 1 cut(s) 618
Bme18I GGWCC 1 cut(s) 283
BmgT120I GGNCC 3 cut(s) 283, 477, 478
BmiI GGNNCC 3 cut(s) 265, 479, 603
BmrFI CCNGG 1 cut(s) 618
BmrI ACTGGG 1 cut(s) 423
BmsI GCATC 1 cut(s) 229
BmuI ACTGGG 1 cut(s) 423
BpmI CTGGAG 2 cut(s) 261, 315
BpuEI CTTGAG 2 cut(s) 206, 646
BsaHI GRCGYC 3 cut(s) 210, 264, 270
BsaI GGTCTC 1 cut(s) 412
BsaJI CCNNGG 1 cut(s) 616
BsaXI ACNNNNNCTCC 2 cut(s) 15, 45
Bsc4I CCNNNNNNNGG 2 cut(s) 159, 487
Bse1I ACTGG 3 cut(s) 332, 418, 630
Bse3DI GCAATG 1 cut(s) 243
BseBI CCWGG 1 cut(s) 618
BseDI CCNNGG 1 cut(s) 616
BseGI GGATG 2 cut(s) 649, 707
BseLI CCNNNNNNNGG 2 cut(s) 159, 487
BseMI GCAATG 1 cut(s) 243
BseMII CTCAG 1 cut(s) 487
BseNI ACTGG 3 cut(s) 332, 418, 630
BseRI GAGGAG 2 cut(s) 43, 242
BseSI GKGCMC 1 cut(s) 481
Bsh1236I CGCG 1 cut(s) 146
Bsh1285I CGRYCG 1 cut(s) 259
BshFI GGCC 3 cut(s) 479, 499, 585
BshNI GGYRCC 2 cut(s) 263, 601
BsiEI CGRYCG 1 cut(s) 259
BslI CCNNNNNNNGG 2 cut(s) 159, 487
BsmAI GTCTC 3 cut(s) 335, 412, 664
BsmI GAATGC 1 cut(s) 544
BsnI GGCC 3 cut(s) 479, 499, 585
Bso31I GGTCTC 1 cut(s) 412
Bsp120I GGGCCC 1 cut(s) 477
Bsp1286I GDGCHC 1 cut(s) 481
Bsp143I GATC 4 cut(s) 256, 298, 434, 703
BspACI CCGC 2 cut(s) 86, 144
BspANI GGCC 3 cut(s) 479, 499, 585
BspCNI CTCAG 1 cut(s) 486
BspFNI CGCG 1 cut(s) 146
BspLI GGNNCC 3 cut(s) 265, 479, 603
BspPI GGATC 1 cut(s) 698
BspT107I GGYRCC 2 cut(s) 263, 601
BspTNI GGTCTC 1 cut(s) 412
BsrBI CCGCTC 1 cut(s) 88
BsrDI GCAATG 1 cut(s) 243
BsrI ACTGG 3 cut(s) 332, 418, 630
BssECI CCNNGG 1 cut(s) 616
BssMI GATC 4 cut(s) 256, 298, 434, 703
BssNI GRCGYC 3 cut(s) 210, 264, 270
Bst2UI CCWGG 1 cut(s) 618
Bst4CI ACNGT 1 cut(s) 550
Bst6I CTCTTC 1 cut(s) 642
BstACI GRCGYC 3 cut(s) 210, 264, 270
BstC8I GCNNGC 3 cut(s) 291, 310, 583
BstDEI CTNAG 1 cut(s) 473
BstEII GGTNACC 1 cut(s) 21
BstF5I GGATG 2 cut(s) 649, 707
BstFNI CGCG 1 cut(s) 146
BstH2I RGCGCY 1 cut(s) 267
BstHHI GCGC 2 cut(s) 148, 266
BstKTI GATC 4 cut(s) 259, 301, 437, 706
BstMAI GTCTC 3 cut(s) 335, 412, 664
BstMBI GATC 4 cut(s) 256, 298, 434, 703
BstMCI CGRYCG 1 cut(s) 259
BstNI CCWGG 1 cut(s) 618
BstPI GGTNACC 1 cut(s) 21
BstSCI CCNGG 1 cut(s) 616
BstSLI GKGCMC 1 cut(s) 481
BstUI CGCG 1 cut(s) 146
BsuRI GGCC 3 cut(s) 479, 499, 585
BtsCI GGATG 2 cut(s) 649, 707
BtsIMutI CAGTG 1 cut(s) 555
Cac8I GCNNGC 3 cut(s) 291, 310, 583
CaiI CAGNNNCTG 1 cut(s) 554
CfoI GCGC 2 cut(s) 148, 266
Cfr13I GGNCC 3 cut(s) 283, 477, 478
CseI GACGC 2 cut(s) 218, 278
CspCI CAANNNNNGTGG 4 cut(s) 444, 479, 531, 566
CviJI RGCY 9 cut(s) 167, 232, 293, 379, 479, 499, 521, 554, 585
CviKI_1 RGCY 9 cut(s) 167, 232, 293, 379, 479, 499, 521, 554, 585
DdeI CTNAG 1 cut(s) 473
DinI GGCGCC 1 cut(s) 265
DpnI GATC 4 cut(s) 258, 300, 436, 705
DpnII GATC 4 cut(s) 256, 298, 434, 703
EaeI YGGCCR 1 cut(s) 497
Eam1104I CTCTTC 1 cut(s) 642
EarI CTCTTC 1 cut(s) 642
Eco147I AGGCCT 1 cut(s) 585
Eco24I GRGCYC 1 cut(s) 481
Eco31I GGTCTC 1 cut(s) 412
Eco47I GGWCC 1 cut(s) 283
Eco91I GGTNACC 1 cut(s) 21
EcoO109I RGGNCCY 1 cut(s) 477
EcoO65I GGTNACC 1 cut(s) 21
EcoRII CCWGG 1 cut(s) 616
EcoT38I GRGCYC 1 cut(s) 481
EgeI GGCGCC 1 cut(s) 265
EheI GGCGCC 1 cut(s) 265
FaiI YATR 2 cut(s) 488, 637
FauI CCCGC 2 cut(s) 93, 151
FbaI TGATCA 1 cut(s) 298
FblI GTMKAC 1 cut(s) 358
FokI GGATG 2 cut(s) 656, 694
FriOI GRGCYC 1 cut(s) 481
FspBI CTAG 1 cut(s) 68
GlaI GCGC 2 cut(s) 147, 265
GsuI CTGGAG 2 cut(s) 261, 315
HaeII RGCGCY 1 cut(s) 267
HaeIII GGCC 3 cut(s) 479, 499, 585
HgaI GACGC 2 cut(s) 218, 278
HhaI GCGC 2 cut(s) 148, 266
Hin1I GRCGYC 3 cut(s) 210, 264, 270
Hin6I GCGC 2 cut(s) 146, 264
HinP1I GCGC 2 cut(s) 146, 264
HinfI GANTC 1 cut(s) 491
HphI GGTGA 5 cut(s) 15, 48, 82, 632, 680
Hpy166II GTNNAC 4 cut(s) 359, 445, 466, 623
Hpy188I TCNGA 3 cut(s) 303, 537, 728
Hpy188III TCNNGA 6 cut(s) 236, 278, 438, 574, 663, 691
Hpy8I GTNNAC 4 cut(s) 359, 445, 466, 623
Hpy99I CGWCG 2 cut(s) 272, 368
HpyAV CCTTC 2 cut(s) 117, 187
HpyCH4III ACNGT 1 cut(s) 550
HpyCH4V TGCA 3 cut(s) 308, 542, 581
HpyF3I CTNAG 1 cut(s) 473
Hsp92I GRCGYC 3 cut(s) 210, 264, 270
HspAI GCGC 2 cut(s) 146, 264
KasI GGCGCC 1 cut(s) 263
Ksp22I TGATCA 1 cut(s) 298
Kzo9I GATC 4 cut(s) 256, 298, 434, 703
LmnI GCTCC 3 cut(s) 36, 229, 376
LweI GCATC 1 cut(s) 229
MaeI CTAG 1 cut(s) 68
MaeIII GTNAC 3 cut(s) 21, 325, 514
MalI GATC 4 cut(s) 258, 300, 436, 705
MbiI CCGCTC 1 cut(s) 88
MboI GATC 4 cut(s) 256, 298, 434, 703
MboII GAAGA 3 cut(s) 3, 6, 659
MhlI GDGCHC 1 cut(s) 481
MlsI TGGCCA 1 cut(s) 499
MluCI AATT 4 cut(s) 171, 560, 630, 695
MluNI TGGCCA 1 cut(s) 499
Mly113I GGCGCC 1 cut(s) 264
MlyI GAGTC 1 cut(s) 500
MnlI CCTC 9 cut(s) 61, 64, 69, 103, 220, 254, 274, 543, 659
Mox20I TGGCCA 1 cut(s) 499
MscI TGGCCA 1 cut(s) 499
MseI TTAA 3 cut(s) 431, 527, 597
Msp20I TGGCCA 1 cut(s) 499
MspR9I CCNGG 1 cut(s) 618
Mva1269I GAATGC 1 cut(s) 544
MvaI CCWGG 1 cut(s) 618
MvnI CGCG 1 cut(s) 146
NarI GGCGCC 1 cut(s) 264
NdeII GATC 4 cut(s) 256, 298, 434, 703
NlaIV GGNNCC 3 cut(s) 265, 479, 603
NmuCI GTSAC 1 cut(s) 21
PceI AGGCCT 1 cut(s) 585
PctI GAATGC 1 cut(s) 544
PflMI CCANNNNNTGG 1 cut(s) 487
Ple19I CGATCG 1 cut(s) 259
PleI GAGTC 1 cut(s) 499
PluTI GGCGCC 1 cut(s) 267
PpsI GAGTC 1 cut(s) 499
Psp6I CCWGG 1 cut(s) 616
PspEI GGTNACC 1 cut(s) 21
PspGI CCWGG 1 cut(s) 616
PspN4I GGNNCC 3 cut(s) 265, 479, 603
PspOMI GGGCCC 1 cut(s) 477
PspPI GGNCC 3 cut(s) 283, 477, 478
PstNI CAGNNNCTG 1 cut(s) 554
PvuI CGATCG 1 cut(s) 259
SaqAI TTAA 3 cut(s) 431, 527, 597
Sau3AI GATC 4 cut(s) 256, 298, 434, 703
Sau96I GGNCC 3 cut(s) 283, 477, 478
SchI GAGTC 1 cut(s) 500
ScrFI CCNGG 1 cut(s) 618
SduI GDGCHC 1 cut(s) 481
SfaNI GCATC 1 cut(s) 229
SfoI GGCGCC 1 cut(s) 265
SinI GGWCC 1 cut(s) 283
SmlI CTYRAG 2 cut(s) 221, 661
SmoI CTYRAG 2 cut(s) 221, 661
SpeI ACTAGT 1 cut(s) 67
Sse9I AATT 4 cut(s) 171, 560, 630, 695
SseBI AGGCCT 1 cut(s) 585
SsiI CCGC 2 cut(s) 86, 144
SspDI GGCGCC 1 cut(s) 263
SspMI CTAG 1 cut(s) 68
StuI AGGCCT 1 cut(s) 585
StyD4I CCNGG 1 cut(s) 616
TaaI ACNGT 1 cut(s) 550
TaqI TCGA 7 cut(s) 194, 237, 255, 259, 363, 437, 715
TasI AATT 4 cut(s) 171, 560, 630, 695
Tru1I TTAA 3 cut(s) 431, 527, 597
Tru9I TTAA 3 cut(s) 431, 527, 597
TscAI CASTG 1 cut(s) 555
TseFI GTSAC 1 cut(s) 21
Tsp45I GTSAC 1 cut(s) 21
TspDTI ATGAA 2 cut(s) 660, 676
TspGWI ACGGA 1 cut(s) 381
TspRI CASTG 1 cut(s) 555
Van91I CCANNNNNTGG 1 cut(s) 487
VpaK11BI GGWCC 1 cut(s) 283
XapI RAATTY 2 cut(s) 171, 560
XmiI GTMKAC 1 cut(s) 358
XspI CTAG 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.