MD15G1421500.v1.1

plastid-lipid-associated protein 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005370
Physical Location & Seq
Reverse (-)
52325120 .. 52329169
4050 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1421500.v1.1.491

Sequence Viewer

Length: 738 bp
ATGGCCTTATCTTGTTCTTGGTCACCTACGCTTCTCACAACCACCCAATCCTCCTCCTCCTCCGCCGCCTCGCCATCGACCCCTAAACTAGTCCCCTGTCATTTTCCGGGGAAACCCACCACCACCACCATTCACACACTCTCCAGCTGCAGTTCAAGTTCTCAGAAATGGAGAGCCAAAGTTTCATTCTTTCCTGCATTTTTGAACAAACGCAAAGACACTCAGCCTATCAAGGACGAGCTTCTTGACGCCATTGCTCCGCTCGATCGAGGCGCTGATGCCACTCCGGAGGACCAGCAAACTGTTGATCAGATTGCACGCAAACTTGAAGCAGTTAACCCAACAAAGGAACCACTCAAATCTGATTTACTCAATGGAAAATGGGAGCTTATATACACTACTTCAAAGTCTATTCTGCAAACTCAGAGGCCCAAAATTTTGAGATCAAAGGTAAACTACCAAGCCATCAATGCTGACACACTTCGGGCTCAAAATATGGAATCATGGCCAACTTTTAATCAGGTAACAGCAGATTTGACACCTTTGAATGCAAGAAAAGTTGCTGTCAAATTCGATTACTTCAAAATTGCAGGTTTGATACCTATTAAGGCGCCTGATAGAGCTCGTGGTGAACTGGAAATTACATATTTGGATGAAGAGCTTCGTGTCTCAAGGGGCGACAAAGGAAACTTGTTCATCTTGAAAATGGTTGATCCATCTTACCGAGTTCCTGTCTGA

Protein Analysis

246

Amino Acids

27.25

Weight (kDa)

9.38

Isoelectric Point (pI)

38.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP_fibrillin PF04755 77 - 235 7.1e-38 PAP_fibrillin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 581
AccB1I GGYRCC 1 cut(s) 610
AccBSI CCGCTC 1 cut(s) 262
AccIII TCCGGA 1 cut(s) 286
AciI CCGC 3 cut(s) 63, 66, 260
AclWI GGATC 1 cut(s) 707
AcoI YGGCCR 1 cut(s) 506
AcsI RAATTY 2 cut(s) 435, 569
AcyI GRCGYC 2 cut(s) 249, 611
AfiI CCNNNNNNNGG 1 cut(s) 346
AgsI TTSAA 7 cut(s) 156, 205, 329, 405, 547, 583, 703
AhlI ACTAGT 1 cut(s) 88
AjuI GAANNNNNNNTTGG 2 cut(s) 170, 202
AluBI AGCT 5 cut(s) 147, 241, 388, 623, 661
AluI AGCT 5 cut(s) 147, 241, 388, 623, 661
Alw21I GWGCWC 1 cut(s) 625
Alw26I GTCTC 1 cut(s) 673
AlwI GGATC 1 cut(s) 707
Aor13HI TCCGGA 1 cut(s) 286
AoxI GGCC 3 cut(s) 3, 428, 506
ApeKI GCWGC 1 cut(s) 147
ApoI RAATTY 2 cut(s) 435, 569
Asp700I GAANNNNTTC 1 cut(s) 660
AspLEI GCGC 2 cut(s) 275, 613
AspS9I GGNCC 2 cut(s) 292, 429
AsuC2I CCSGG 1 cut(s) 108
AsuHPI GGTGA 2 cut(s) 15, 641
AvaII GGWCC 1 cut(s) 292
BalI TGGCCA 1 cut(s) 508
BanI GGYRCC 1 cut(s) 610
BanII GRGCYC 2 cut(s) 490, 625
BauI CACGAG 1 cut(s) 624
Bbv12I GWGCWC 1 cut(s) 625
BbvI GCAGC 1 cut(s) 134
BccI CCATC 3 cut(s) 82, 473, 724
BclI TGATCA 1 cut(s) 307
BcnI CCSGG 1 cut(s) 108
BcoDI GTCTC 1 cut(s) 673
BcuI ACTAGT 1 cut(s) 88
BfaI CTAG 1 cut(s) 89
BfmI CTRYAG 1 cut(s) 148
BfoI RGCGCY 2 cut(s) 276, 614
BfuAI ACCTGC 1 cut(s) 581
BisI GCNGC 2 cut(s) 66, 148
BlsI GCNGC 2 cut(s) 67, 149
Bme1390I CCNGG 1 cut(s) 108
Bme18I GGWCC 1 cut(s) 292
BmgT120I GGNCC 2 cut(s) 292, 429
BmiI GGNNCC 2 cut(s) 351, 612
BmrFI CCNGG 1 cut(s) 108
BmsI GCATC 1 cut(s) 268
BpmI CTGGAG 1 cut(s) 127
BpuEI CTTGAG 1 cut(s) 655
BpuMI CCSGG 1 cut(s) 108
BsaHI GRCGYC 2 cut(s) 249, 611
BsaJI CCNNGG 1 cut(s) 107
BsaWI WCCGGW 1 cut(s) 286
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bsc4I CCNNNNNNNGG 1 cut(s) 346
Bse1I ACTGG 1 cut(s) 639
Bse3DI GCAATG 1 cut(s) 252
BseAI TCCGGA 1 cut(s) 286
BseDI CCNNGG 1 cut(s) 107
BseGI GGATG 1 cut(s) 658
BseLI CCNNNNNNNGG 1 cut(s) 346
BseMI GCAATG 1 cut(s) 252
BseMII CTCAG 3 cut(s) 176, 236, 437
BseNI ACTGG 1 cut(s) 639
BseRI GAGGAG 3 cut(s) 43, 46, 49
BseXI GCAGC 1 cut(s) 134
Bsh1285I CGRYCG 1 cut(s) 268
BshFI GGCC 3 cut(s) 5, 430, 508
BshNI GGYRCC 1 cut(s) 610
BsiEI CGRYCG 1 cut(s) 268
BsiHKAI GWGCWC 1 cut(s) 625
BsiSI CCGG 2 cut(s) 107, 287
BslFI GGGAC 1 cut(s) 77
BslI CCNNNNNNNGG 1 cut(s) 346
BsmAI GTCTC 1 cut(s) 673
BsmFI GGGAC 1 cut(s) 77
BsmI GAATGC 1 cut(s) 553
BsnI GGCC 3 cut(s) 5, 430, 508
Bsp1286I GDGCHC 2 cut(s) 490, 625
Bsp13I TCCGGA 1 cut(s) 286
Bsp143I GATC 4 cut(s) 265, 307, 443, 712
BspACI CCGC 3 cut(s) 63, 66, 260
BspANI GGCC 3 cut(s) 5, 430, 508
BspCNI CTCAG 3 cut(s) 175, 235, 436
BspEI TCCGGA 1 cut(s) 286
BspLI GGNNCC 2 cut(s) 351, 612
BspMAI CTGCAG 1 cut(s) 152
BspMI ACCTGC 1 cut(s) 581
BspPI GGATC 1 cut(s) 707
BspQI GCTCTTC 1 cut(s) 651
BspT107I GGYRCC 1 cut(s) 610
BsrBI CCGCTC 1 cut(s) 262
BsrDI GCAATG 1 cut(s) 252
BsrI ACTGG 1 cut(s) 639
BssECI CCNNGG 1 cut(s) 107
BssMI GATC 4 cut(s) 265, 307, 443, 712
BssNI GRCGYC 2 cut(s) 249, 611
BssSI CACGAG 1 cut(s) 624
Bst2BI CACGAG 1 cut(s) 624
Bst4CI ACNGT 1 cut(s) 304
Bst6I CTCTTC 1 cut(s) 651
BstACI GRCGYC 2 cut(s) 249, 611
BstC8I GCNNGC 1 cut(s) 319
BstDEI CTNAG 3 cut(s) 162, 222, 423
BstEII GGTNACC 1 cut(s) 21
BstF5I GGATG 1 cut(s) 658
BstH2I RGCGCY 2 cut(s) 276, 614
BstHHI GCGC 2 cut(s) 275, 613
BstKTI GATC 4 cut(s) 268, 310, 446, 715
BstMAI GTCTC 1 cut(s) 673
BstMBI GATC 4 cut(s) 265, 307, 443, 712
BstMCI CGRYCG 1 cut(s) 268
BstMWI GCNNNNNNNGC 1 cut(s) 470
BstPI GGTNACC 1 cut(s) 21
BstSCI CCNGG 1 cut(s) 106
BstSFI CTRYAG 1 cut(s) 148
BstV1I GCAGC 1 cut(s) 134
BsuRI GGCC 3 cut(s) 5, 430, 508
BtsCI GGATG 1 cut(s) 658
BveI ACCTGC 1 cut(s) 581
Cac8I GCNNGC 1 cut(s) 319
CfoI GCGC 2 cut(s) 275, 613
Cfr13I GGNCC 2 cut(s) 292, 429
CseI GACGC 1 cut(s) 257
CviAII CATG 1 cut(s) 504
DdeI CTNAG 3 cut(s) 162, 222, 423
DinI GGCGCC 1 cut(s) 612
DpnI GATC 4 cut(s) 267, 309, 445, 714
DpnII GATC 4 cut(s) 265, 307, 443, 712
EaeI YGGCCR 1 cut(s) 506
Eam1104I CTCTTC 1 cut(s) 651
EarI CTCTTC 1 cut(s) 651
EciI GGCGGA 1 cut(s) 52
Ecl136II GAGCTC 1 cut(s) 623
Eco24I GRGCYC 2 cut(s) 490, 625
Eco47I GGWCC 1 cut(s) 292
Eco53kI GAGCTC 1 cut(s) 623
Eco91I GGTNACC 1 cut(s) 21
EcoICRI GAGCTC 1 cut(s) 623
EcoO65I GGTNACC 1 cut(s) 21
EcoT38I GRGCYC 2 cut(s) 490, 625
EgeI GGCGCC 1 cut(s) 612
EheI GGCGCC 1 cut(s) 612
FaeI CATG 1 cut(s) 507
FaiI YATR 5 cut(s) 392, 394, 497, 505, 646
FaqI GGGAC 1 cut(s) 77
FatI CATG 1 cut(s) 503
FbaI TGATCA 1 cut(s) 307
Fnu4HI GCNGC 2 cut(s) 66, 148
FokI GGATG 1 cut(s) 665
FriOI GRGCYC 2 cut(s) 490, 625
Fsp4HI GCNGC 2 cut(s) 66, 148
FspBI CTAG 1 cut(s) 89
GlaI GCGC 2 cut(s) 274, 612
GluI GCNGC 2 cut(s) 66, 148
GsuI CTGGAG 1 cut(s) 127
HaeII RGCGCY 2 cut(s) 276, 614
HaeIII GGCC 3 cut(s) 5, 430, 508
HapII CCGG 2 cut(s) 107, 287
HgaI GACGC 1 cut(s) 257
HhaI GCGC 2 cut(s) 275, 613
Hin1I GRCGYC 2 cut(s) 249, 611
Hin1II CATG 1 cut(s) 507
Hin6I GCGC 2 cut(s) 273, 611
HinP1I GCGC 2 cut(s) 273, 611
HincII GTYRAC 1 cut(s) 337
HindII GTYRAC 1 cut(s) 337
HinfI GANTC 1 cut(s) 500
HpaI GTTAAC 1 cut(s) 337
HpaII CCGG 2 cut(s) 107, 287
HphI GGTGA 2 cut(s) 15, 641
Hpy166II GTNNAC 3 cut(s) 337, 454, 632
Hpy188I TCNGA 5 cut(s) 165, 312, 364, 426, 737
Hpy188III TCNNGA 3 cut(s) 245, 287, 700
Hpy8I GTNNAC 3 cut(s) 337, 454, 632
HpyCH4III ACNGT 1 cut(s) 304
HpyCH4V TGCA 6 cut(s) 150, 197, 317, 418, 551, 590
HpyF10VI GCNNNNNNNGC 1 cut(s) 470
HpyF3I CTNAG 3 cut(s) 162, 222, 423
Hsp92I GRCGYC 2 cut(s) 249, 611
Hsp92II CATG 1 cut(s) 507
HspAI GCGC 2 cut(s) 273, 611
KasI GGCGCC 1 cut(s) 610
Kpn2I TCCGGA 1 cut(s) 286
Ksp22I TGATCA 1 cut(s) 307
KspAI GTTAAC 1 cut(s) 337
Kzo9I GATC 4 cut(s) 265, 307, 443, 712
LguI GCTCTTC 1 cut(s) 651
LmnI GCTCC 2 cut(s) 262, 385
Lsp1109I GCAGC 1 cut(s) 134
LweI GCATC 1 cut(s) 268
MaeI CTAG 1 cut(s) 89
MaeIII GTNAC 2 cut(s) 21, 523
MalI GATC 4 cut(s) 267, 309, 445, 714
MbiI CCGCTC 1 cut(s) 262
MboI GATC 4 cut(s) 265, 307, 443, 712
MboII GAAGA 1 cut(s) 668
MhlI GDGCHC 2 cut(s) 490, 625
MlsI TGGCCA 1 cut(s) 508
MluCI AATT 4 cut(s) 435, 569, 585, 639
MluNI TGGCCA 1 cut(s) 508
Mly113I GGCGCC 1 cut(s) 611
MnlI CCTC 8 cut(s) 61, 64, 67, 70, 79, 263, 283, 420
Mox20I TGGCCA 1 cut(s) 508
MroI TCCGGA 1 cut(s) 286
MroXI GAANNNNTTC 1 cut(s) 660
MscI TGGCCA 1 cut(s) 508
MseI TTAA 3 cut(s) 336, 516, 606
Msp20I TGGCCA 1 cut(s) 508
MspA1I CMGCKG 1 cut(s) 147
MspI CCGG 2 cut(s) 107, 287
MspR9I CCNGG 1 cut(s) 108
Mva1269I GAATGC 1 cut(s) 553
MwoI GCNNNNNNNGC 1 cut(s) 470
NarI GGCGCC 1 cut(s) 611
NciI CCSGG 1 cut(s) 108
NdeII GATC 4 cut(s) 265, 307, 443, 712
NlaIII CATG 1 cut(s) 507
NlaIV GGNNCC 2 cut(s) 351, 612
NmuCI GTSAC 1 cut(s) 21
PciSI GCTCTTC 1 cut(s) 651
PctI GAATGC 1 cut(s) 553
PdmI GAANNNNTTC 1 cut(s) 660
PfeI GAWTC 1 cut(s) 500
PkrI GCNGC 2 cut(s) 67, 149
Ple19I CGATCG 1 cut(s) 268
PluTI GGCGCC 1 cut(s) 614
Psp124BI GAGCTC 1 cut(s) 625
PspEI GGTNACC 1 cut(s) 21
PspN4I GGNNCC 2 cut(s) 351, 612
PspPI GGNCC 2 cut(s) 292, 429
PstI CTGCAG 1 cut(s) 152
PvuI CGATCG 1 cut(s) 268
PvuII CAGCTG 1 cut(s) 147
SacI GAGCTC 1 cut(s) 625
SapI GCTCTTC 1 cut(s) 651
SaqAI TTAA 3 cut(s) 336, 516, 606
SatI GCNGC 2 cut(s) 66, 148
Sau3AI GATC 4 cut(s) 265, 307, 443, 712
Sau96I GGNCC 2 cut(s) 292, 429
ScrFI CCNGG 1 cut(s) 108
SduI GDGCHC 2 cut(s) 490, 625
SfaNI GCATC 1 cut(s) 268
SfcI CTRYAG 1 cut(s) 148
SfoI GGCGCC 1 cut(s) 612
SinI GGWCC 1 cut(s) 292
SmlI CTYRAG 1 cut(s) 670
SmoI CTYRAG 1 cut(s) 670
SpeI ACTAGT 1 cut(s) 88
Sse9I AATT 4 cut(s) 435, 569, 585, 639
SsiI CCGC 3 cut(s) 63, 66, 260
SspDI GGCGCC 1 cut(s) 610
SspMI CTAG 1 cut(s) 89
SstI GAGCTC 1 cut(s) 625
StyD4I CCNGG 1 cut(s) 106
TaaI ACNGT 1 cut(s) 304
TaqI TCGA 4 cut(s) 77, 264, 268, 573
TasI AATT 4 cut(s) 435, 569, 585, 639
TauI GCSGC 1 cut(s) 68
TfiI GAWTC 1 cut(s) 500
Tru1I TTAA 3 cut(s) 336, 516, 606
Tru9I TTAA 3 cut(s) 336, 516, 606
TseFI GTSAC 1 cut(s) 21
TseI GCWGC 1 cut(s) 147
Tsp45I GTSAC 1 cut(s) 21
TspDTI ATGAA 3 cut(s) 174, 669, 685
VpaK11BI GGWCC 1 cut(s) 292
XapI RAATTY 2 cut(s) 435, 569
XmnI GAANNNNTTC 1 cut(s) 660
XspI CTAG 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.