MD15G1421400.v1.1

PAP_fibrillin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005370
Physical Location & Seq
Reverse (-)
52321609 .. 52324778
3170 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1421400.v1.1.491

Sequence Viewer

Length: 756 bp
ATGGCTCTCTCTTCACTCAGTCATGCCACTCCACTGAAAACTCATGGATTCTCGTTCAAATACCCCTTTGATTCTTCACCTTCACTCATTGCTTCTTTCTCACCGAAACCAAACCCTACCAGTTCCTGCATAATACCAAGCCACAACAACTTGCTGGGCAAAATTATTCTTGATTCTTTGAAATGGAGGACCGGAGTTTCCTTCCTTTCAGGATTTCCTACGAAAGGTGAAGACATCAACAGCCTCAAGGAAGAGCTTTACAACACAATAGCTCCTCTTGATAGGGGTGCCGAAGCTACCCTGCAGGACCAAGAACGCGTAGAAGAGATTGCACGTAAGCTTGAGGCACTCAACGAAATAAAAGAACCTCTCAAGTCTAGTCTGTTAAATGGAAAATGGGAGCTCATATATACAACATCTCAGTCAGTTCTGCAAACACAGAGGCCGAAGTTCTTACGACCAAATGGAAAGATCTATCAGGCAATCAATGCAGATACACTAAGGGCTCAGAATATCGAAACTTGGCCCTTCTTTAACCAGGTTACAGCCAATTTAGTCCCTTTAAACTCCAGAAGGGTGGCAGTTAAATTTGATTTCTTCCGGATTGCTAGTCTGATACCTATAAAAAATCCTGGAAGCGGCCGCGGTCAGTTAGAAATTACATACTTGGATGAGGAGTTACGAATATCAAGAGGCGATAGAGGGAACTTGTTCATATTGAAGATGGTTGATCCAACCTATAGGGTCCCTCTCTGA

Protein Analysis

252

Amino Acids

28.23

Weight (kDa)

9.38

Isoelectric Point (pI)

40.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP_fibrillin PF04755 81 - 241 5.9e-35 PAP_fibrillin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 287
AccII CGCG 2 cut(s) 318, 645
AccIII TCCGGA 1 cut(s) 600
AciI CCGC 3 cut(s) 639, 643, 645
AclWI GGATC 1 cut(s) 725
AcoI YGGCCR 1 cut(s) 640
AcsI RAATTY 1 cut(s) 587
AfiI CCNNNNNNNGG 2 cut(s) 224, 638
AflIII ACRYGT 1 cut(s) 316
AgsI TTSAA 3 cut(s) 58, 181, 721
AjnI CCWGG 2 cut(s) 537, 631
AluBI AGCT 5 cut(s) 256, 272, 296, 340, 403
AluI AGCT 5 cut(s) 256, 272, 296, 340, 403
Alw21I GWGCWC 1 cut(s) 405
AlwI GGATC 1 cut(s) 725
AlwNI CAGNNNCTG 1 cut(s) 126
Aor13HI TCCGGA 1 cut(s) 600
AoxI GGCC 3 cut(s) 443, 524, 640
ApoI RAATTY 1 cut(s) 587
Asp700I GAANNNNTTC 1 cut(s) 710
AspS9I GGNCC 4 cut(s) 189, 307, 525, 745
AsuHPI GGTGA 3 cut(s) 69, 93, 239
AvaII GGWCC 3 cut(s) 189, 307, 745
BanI GGYRCC 1 cut(s) 287
BanII GRGCYC 2 cut(s) 405, 508
BbsI GAAGAC 1 cut(s) 237
Bbv12I GWGCWC 1 cut(s) 405
BccI CCATC 1 cut(s) 718
BciT130I CCWGG 2 cut(s) 539, 633
BfaI CTAG 2 cut(s) 378, 609
BfmI CTRYAG 2 cut(s) 302, 739
BglII AGATCT 1 cut(s) 471
BisI GCNGC 2 cut(s) 640, 643
BlsI GCNGC 2 cut(s) 641, 644
Bme1390I CCNGG 2 cut(s) 539, 633
Bme18I GGWCC 3 cut(s) 189, 307, 745
BmgT120I GGNCC 4 cut(s) 189, 307, 525, 745
BmiI GGNNCC 3 cut(s) 289, 746, 747
BmrFI CCNGG 2 cut(s) 539, 633
BpiI GAAGAC 1 cut(s) 237
BpmI CTGGAG 1 cut(s) 553
BpuEI CTTGAG 3 cut(s) 230, 356, 362
BsaAI YACGTR 1 cut(s) 335
BsaJI CCNNGG 1 cut(s) 643
BsaWI WCCGGW 2 cut(s) 191, 600
BsaXI ACNNNNNCTCC 2 cut(s) 256, 286
Bsc4I CCNNNNNNNGG 2 cut(s) 224, 638
Bse1I ACTGG 1 cut(s) 120
Bse3DI GCAATG 1 cut(s) 87
BseAI TCCGGA 1 cut(s) 600
BseBI CCWGG 2 cut(s) 539, 633
BseDI CCNNGG 1 cut(s) 643
BseGI GGATG 1 cut(s) 676
BseLI CCNNNNNNNGG 2 cut(s) 224, 638
BseMI GCAATG 1 cut(s) 87
BseMII CTCAG 3 cut(s) 31, 434, 521
BseNI ACTGG 1 cut(s) 120
BseRI GAGGAG 2 cut(s) 264, 689
BseX3I CGGCCG 1 cut(s) 640
BseYI CCCAGC 1 cut(s) 154
Bsh1236I CGCG 2 cut(s) 318, 645
Bsh1285I CGRYCG 1 cut(s) 643
BshFI GGCC 3 cut(s) 445, 526, 642
BshNI GGYRCC 1 cut(s) 287
BsiEI CGRYCG 1 cut(s) 643
BsiHKAI GWGCWC 1 cut(s) 405
BsiSI CCGG 2 cut(s) 192, 601
BslFI GGGAC 2 cut(s) 542, 731
BslI CCNNNNNNNGG 2 cut(s) 224, 638
BsmFI GGGAC 2 cut(s) 542, 731
BsnI GGCC 3 cut(s) 445, 526, 642
Bsp1286I GDGCHC 2 cut(s) 405, 508
Bsp13I TCCGGA 1 cut(s) 600
Bsp143I GATC 2 cut(s) 471, 730
BspACI CCGC 3 cut(s) 639, 643, 645
BspANI GGCC 3 cut(s) 445, 526, 642
BspCNI CTCAG 3 cut(s) 30, 433, 520
BspEI TCCGGA 1 cut(s) 600
BspFNI CGCG 2 cut(s) 318, 645
BspLI GGNNCC 3 cut(s) 289, 746, 747
BspMAI CTGCAG 1 cut(s) 306
BspPI GGATC 1 cut(s) 725
BspQI GCTCTTC 1 cut(s) 246
BspT107I GGYRCC 1 cut(s) 287
BsrDI GCAATG 1 cut(s) 87
BsrI ACTGG 1 cut(s) 120
BssECI CCNNGG 1 cut(s) 643
BssMI GATC 2 cut(s) 471, 730
Bst2UI CCWGG 2 cut(s) 539, 633
Bst6I CTCTTC 3 cut(s) 16, 246, 318
BstAPI GCANNNNNTGC 1 cut(s) 488
BstBAI YACGTR 1 cut(s) 335
BstDEI CTNAG 4 cut(s) 17, 420, 500, 507
BstDSI CCRYGG 1 cut(s) 643
BstENI CCTNNNNNAGG 1 cut(s) 222
BstF5I GGATG 1 cut(s) 676
BstFNI CGCG 2 cut(s) 318, 645
BstKTI GATC 2 cut(s) 474, 733
BstMBI GATC 2 cut(s) 471, 730
BstMCI CGRYCG 1 cut(s) 643
BstMWI GCNNNNNNNGC 1 cut(s) 488
BstNI CCWGG 2 cut(s) 539, 633
BstSCI CCNGG 2 cut(s) 537, 631
BstSFI CTRYAG 2 cut(s) 302, 739
BstUI CGCG 2 cut(s) 318, 645
BstV2I GAAGAC 1 cut(s) 237
BstX2I RGATCY 1 cut(s) 471
BstXI CCANNNNNNTGG 1 cut(s) 577
BstYI RGATCY 1 cut(s) 471
BstZI CGGCCG 1 cut(s) 640
BsuRI GGCC 3 cut(s) 445, 526, 642
BtgI CCRYGG 1 cut(s) 643
BtsCI GGATG 1 cut(s) 676
BtsIMutI CAGTG 1 cut(s) 32
CaiI CAGNNNCTG 1 cut(s) 126
CciNI GCGGCCGC 1 cut(s) 640
Cfr13I GGNCC 4 cut(s) 189, 307, 525, 745
Cfr42I CCGCGG 1 cut(s) 646
CsiI ACCWGGT 1 cut(s) 537
CviAII CATG 2 cut(s) 23, 44
DdeI CTNAG 4 cut(s) 17, 420, 500, 507
DpnI GATC 2 cut(s) 473, 732
DpnII GATC 2 cut(s) 471, 730
DraI TTTAAA 1 cut(s) 564
EaeI YGGCCR 1 cut(s) 640
EagI CGGCCG 1 cut(s) 640
Eam1104I CTCTTC 3 cut(s) 16, 246, 318
EarI CTCTTC 3 cut(s) 16, 246, 318
Ecl136II GAGCTC 1 cut(s) 403
EclXI CGGCCG 1 cut(s) 640
Eco24I GRGCYC 2 cut(s) 405, 508
Eco47I GGWCC 3 cut(s) 189, 307, 745
Eco52I CGGCCG 1 cut(s) 640
Eco53kI GAGCTC 1 cut(s) 403
EcoICRI GAGCTC 1 cut(s) 403
EcoNI CCTNNNNNAGG 1 cut(s) 222
EcoO109I RGGNCCY 1 cut(s) 745
EcoRII CCWGG 2 cut(s) 537, 631
EcoT38I GRGCYC 2 cut(s) 405, 508
FaeI CATG 2 cut(s) 26, 47
FaqI GGGAC 2 cut(s) 542, 731
FatI CATG 2 cut(s) 22, 43
Fnu4HI GCNGC 2 cut(s) 640, 643
FokI GGATG 1 cut(s) 683
FriOI GRGCYC 2 cut(s) 405, 508
Fsp4HI GCNGC 2 cut(s) 640, 643
FspBI CTAG 2 cut(s) 378, 609
GluI GCNGC 2 cut(s) 640, 643
GsaI CCCAGC 1 cut(s) 158
GsuI CTGGAG 1 cut(s) 553
HaeIII GGCC 3 cut(s) 445, 526, 642
HapII CCGG 2 cut(s) 192, 601
Hin1II CATG 2 cut(s) 26, 47
HindIII AAGCTT 1 cut(s) 338
HinfI GANTC 3 cut(s) 48, 71, 173
HpaII CCGG 2 cut(s) 192, 601
HphI GGTGA 3 cut(s) 69, 93, 239
Hpy188I TCNGA 3 cut(s) 510, 615, 755
Hpy188III TCNNGA 6 cut(s) 170, 210, 278, 570, 601, 690
HpyAV CCTTC 4 cut(s) 90, 211, 538, 567
HpyCH4IV ACGT 1 cut(s) 334
HpyCH4V TGCA 5 cut(s) 129, 304, 332, 433, 491
HpyF10VI GCNNNNNNNGC 1 cut(s) 488
HpyF3I CTNAG 4 cut(s) 17, 420, 500, 507
HpySE526I ACGT 1 cut(s) 334
Hsp92II CATG 2 cut(s) 26, 47
KflI GGGWCCC 1 cut(s) 745
Kpn2I TCCGGA 1 cut(s) 600
KspI CCGCGG 1 cut(s) 646
Kzo9I GATC 2 cut(s) 471, 730
LguI GCTCTTC 1 cut(s) 246
LmnI GCTCC 2 cut(s) 277, 400
MabI ACCWGGT 1 cut(s) 537
MaeI CTAG 2 cut(s) 378, 609
MaeII ACGT 1 cut(s) 334
MaeIII GTNAC 2 cut(s) 541, 678
MalI GATC 2 cut(s) 473, 732
MboI GATC 2 cut(s) 471, 730
MboII GAAGA 7 cut(s) 3, 66, 242, 263, 335, 589, 733
MflI RGATCY 1 cut(s) 471
MhlI GDGCHC 2 cut(s) 405, 508
MluCI AATT 4 cut(s) 162, 550, 587, 657
MluI ACGCGT 1 cut(s) 316
MnlI CCTC 9 cut(s) 180, 254, 285, 337, 378, 435, 667, 686, 695
MroI TCCGGA 1 cut(s) 600
MroXI GAANNNNTTC 1 cut(s) 710
MseI TTAA 4 cut(s) 386, 534, 563, 585
MspA1I CMGCKG 1 cut(s) 645
MspI CCGG 2 cut(s) 192, 601
MspR9I CCNGG 2 cut(s) 539, 633
MvaI CCWGG 2 cut(s) 539, 633
MvnI CGCG 2 cut(s) 318, 645
MwoI GCNNNNNNNGC 1 cut(s) 488
NdeII GATC 2 cut(s) 471, 730
NlaIII CATG 2 cut(s) 26, 47
NlaIV GGNNCC 3 cut(s) 289, 746, 747
NotI GCGGCCGC 1 cut(s) 640
PciSI GCTCTTC 1 cut(s) 246
PdmI GAANNNNTTC 1 cut(s) 710
PfeI GAWTC 3 cut(s) 48, 71, 173
PfoI TCCNGGA 1 cut(s) 631
PkrI GCNGC 2 cut(s) 641, 644
Ppu21I YACGTR 1 cut(s) 335
PpuMI RGGWCCY 1 cut(s) 745
Psp124BI GAGCTC 1 cut(s) 405
Psp5II RGGWCCY 1 cut(s) 745
Psp6I CCWGG 2 cut(s) 537, 631
PspFI CCCAGC 1 cut(s) 154
PspGI CCWGG 2 cut(s) 537, 631
PspN4I GGNNCC 3 cut(s) 289, 746, 747
PspPI GGNCC 4 cut(s) 189, 307, 525, 745
PspPPI RGGWCCY 1 cut(s) 745
PstI CTGCAG 1 cut(s) 306
PstNI CAGNNNCTG 1 cut(s) 126
PsuI RGATCY 1 cut(s) 471
SacI GAGCTC 1 cut(s) 405
SacII CCGCGG 1 cut(s) 646
SapI GCTCTTC 1 cut(s) 246
SaqAI TTAA 4 cut(s) 386, 534, 563, 585
SatI GCNGC 2 cut(s) 640, 643
Sau3AI GATC 2 cut(s) 471, 730
Sau96I GGNCC 4 cut(s) 189, 307, 525, 745
SbfI CCTGCAGG 1 cut(s) 306
ScrFI CCNGG 2 cut(s) 539, 633
SdaI CCTGCAGG 1 cut(s) 306
SduI GDGCHC 2 cut(s) 405, 508
SexAI ACCWGGT 1 cut(s) 537
SfcI CTRYAG 2 cut(s) 302, 739
Sfr303I CCGCGG 1 cut(s) 646
SgrBI CCGCGG 1 cut(s) 646
SinI GGWCC 3 cut(s) 189, 307, 745
SmlI CTYRAG 3 cut(s) 245, 341, 371
SmoI CTYRAG 3 cut(s) 245, 341, 371
Sse8387I CCTGCAGG 1 cut(s) 306
Sse9I AATT 4 cut(s) 162, 550, 587, 657
SsiI CCGC 3 cut(s) 639, 643, 645
SspMI CTAG 2 cut(s) 378, 609
SstI GAGCTC 1 cut(s) 405
StyD4I CCNGG 2 cut(s) 537, 631
TaiI ACGT 1 cut(s) 337
TaqI TCGA 1 cut(s) 516
TasI AATT 4 cut(s) 162, 550, 587, 657
TauI GCSGC 2 cut(s) 642, 645
TfiI GAWTC 3 cut(s) 48, 71, 173
Tru1I TTAA 4 cut(s) 386, 534, 563, 585
Tru9I TTAA 4 cut(s) 386, 534, 563, 585
TscAI CASTG 1 cut(s) 39
TspDTI ATGAA 1 cut(s) 703
TspRI CASTG 1 cut(s) 39
VpaK11BI GGWCC 3 cut(s) 189, 307, 745
XagI CCTNNNNNAGG 1 cut(s) 222
XapI RAATTY 1 cut(s) 587
XmnI GAANNNNTTC 1 cut(s) 710
XspI CTAG 2 cut(s) 378, 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.