RchiOBHm_Chr7g0222941

plastid-lipid-associated protein 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0005370
Physical Location & Seq
Forward (+)
44538648 .. 44543584
4937 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19959

Sequence Viewer

Length: 732 bp
ATGGCATTATCTTCTTCCTGGTCACCTACGCTCCTTACAACCACCCAGTTCTCCTCACCTCCCAAACTAGTCCATTCTCATTCCCCGCTCACCTCCTTGACCTTCCCTTCCAAACCCACCACCACCACCACCCGCACCGTTGTTCCCGCGCTTTCCCACAAATGGAGAGCCAGAATTTCCTTCTTTCCTGCGTTTTTGAGCAAAGGCAAAGACGCCAAAACTCTCAAGGAGGAGCTTCTCGATGCCATTGCTTCGCTCGATCGAGGCGCCGACGCCACTCCAGAGGACCAGCTAGCTGTTGATCAGATTACACGCAAACTTGAAGCAGTTACTCCAGTAAAGGAGCCACTCAAATCTGGTCTACTCGATGGAAAATGGGAGCTTCTCTATACTACTTCAAAATCTATTCTCCAAACTGGGAGACCCAAGTTTTTAAGATCAAGAGTAAACTACCAAGCAATCAATGTGGACACACTCAGGGCCCAAAATATGGAGTCGTGGCCAACTTTCAATCAGGTAACAGCCGATTTAACACCTCTGAATGCAAGAAAAGTGGCTGTAAAATTCGACTATTTCAAGATTGCAGGCCTGATACCTGTTAAGGCACCTGACAGAGCCCGTGGTGAACTGGAAATTACATATTTGGATGAAGAGTTGCGTGTCTCAAGAGGCGATAAAGGAAACCTGTTCATCTTGAAATTGGTTGATCCATCCTATCGAGTTCCTGTCTGA

Protein Analysis

243

Amino Acids

27.09

Weight (kDa)

9.69

Isoelectric Point (pI)

31.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP_fibrillin PF04755 74 - 233 1.4e-35 PAP_fibrillin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 266, 604
AccB7I CCANNNNNTGG 1 cut(s) 490
AccBSI CCGCTC 1 cut(s) 88
AccI GTMKAC 1 cut(s) 361
AccII CGCG 1 cut(s) 149
AciI CCGC 3 cut(s) 86, 133, 147
AclWI GGATC 1 cut(s) 701
AcoI YGGCCR 1 cut(s) 500
AcsI RAATTY 2 cut(s) 174, 563
AcyI GRCGYC 3 cut(s) 213, 267, 273
AfiI CCNNNNNNNGG 2 cut(s) 162, 490
AgsI TTSAA 5 cut(s) 323, 399, 511, 577, 697
AhlI ACTAGT 1 cut(s) 67
AjnI CCWGG 1 cut(s) 17
AluBI AGCT 4 cut(s) 235, 292, 296, 382
AluI AGCT 4 cut(s) 235, 292, 296, 382
Alw26I GTCTC 2 cut(s) 415, 667
AlwI GGATC 1 cut(s) 701
AoxI GGCC 3 cut(s) 480, 500, 586
ApaI GGGCCC 1 cut(s) 484
ApoI RAATTY 2 cut(s) 174, 563
AspLEI GCGC 2 cut(s) 151, 269
AspS9I GGNCC 3 cut(s) 286, 480, 481
AsuHPI GGTGA 4 cut(s) 15, 48, 82, 635
AsuNHI GCTAGC 1 cut(s) 292
AvaII GGWCC 1 cut(s) 286
BaeGI GKGCMC 1 cut(s) 484
BalI TGGCCA 1 cut(s) 502
BanI GGYRCC 2 cut(s) 266, 604
BanII GRGCYC 2 cut(s) 484, 619
BccI CCATC 2 cut(s) 362, 718
BcgI CGANNNNNNTGC 2 cut(s) 230, 264
BciT130I CCWGG 1 cut(s) 19
BclI TGATCA 1 cut(s) 301
BcoDI GTCTC 2 cut(s) 415, 667
BcuI ACTAGT 1 cut(s) 67
BfaI CTAG 2 cut(s) 68, 293
BfoI RGCGCY 1 cut(s) 270
Bme1390I CCNGG 1 cut(s) 19
Bme18I GGWCC 1 cut(s) 286
BmgT120I GGNCC 3 cut(s) 286, 480, 481
BmiI GGNNCC 4 cut(s) 268, 345, 482, 606
BmrFI CCNGG 1 cut(s) 19
BmrI ACTGGG 2 cut(s) 40, 426
BmsI GCATC 1 cut(s) 232
BmtI GCTAGC 1 cut(s) 296
BmuI ACTGGG 2 cut(s) 40, 426
BpmI CTGGAG 2 cut(s) 264, 318
BpuEI CTTGAG 2 cut(s) 209, 649
BsaHI GRCGYC 3 cut(s) 213, 267, 273
BsaI GGTCTC 1 cut(s) 415
BsaJI CCNNGG 1 cut(s) 619
BsaXI ACNNNNNCTCC 2 cut(s) 15, 45
Bsc4I CCNNNNNNNGG 2 cut(s) 162, 490
Bse1I ACTGG 4 cut(s) 46, 335, 421, 633
Bse3DI GCAATG 1 cut(s) 246
BseBI CCWGG 1 cut(s) 19
BseDI CCNNGG 1 cut(s) 619
BseGI GGATG 2 cut(s) 652, 710
BseLI CCNNNNNNNGG 2 cut(s) 162, 490
BseMI GCAATG 1 cut(s) 246
BseMII CTCAG 1 cut(s) 490
BseNI ACTGG 4 cut(s) 46, 335, 421, 633
BseRI GAGGAG 2 cut(s) 43, 245
BseSI GKGCMC 1 cut(s) 484
Bsh1236I CGCG 1 cut(s) 149
Bsh1285I CGRYCG 1 cut(s) 262
BshFI GGCC 3 cut(s) 482, 502, 588
BshNI GGYRCC 2 cut(s) 266, 604
BsiEI CGRYCG 1 cut(s) 262
BslI CCNNNNNNNGG 2 cut(s) 162, 490
BsmAI GTCTC 2 cut(s) 415, 667
BsmI GAATGC 1 cut(s) 547
BsnI GGCC 3 cut(s) 482, 502, 588
Bso31I GGTCTC 1 cut(s) 415
Bsp120I GGGCCC 1 cut(s) 480
Bsp1286I GDGCHC 2 cut(s) 484, 619
Bsp143I GATC 4 cut(s) 259, 301, 437, 706
BspACI CCGC 3 cut(s) 86, 133, 147
BspANI GGCC 3 cut(s) 482, 502, 588
BspCNI CTCAG 1 cut(s) 489
BspFNI CGCG 1 cut(s) 149
BspLI GGNNCC 4 cut(s) 268, 345, 482, 606
BspOI GCTAGC 1 cut(s) 296
BspPI GGATC 1 cut(s) 701
BspT107I GGYRCC 2 cut(s) 266, 604
BspTNI GGTCTC 1 cut(s) 415
BsrBI CCGCTC 1 cut(s) 88
BsrDI GCAATG 1 cut(s) 246
BsrI ACTGG 4 cut(s) 46, 335, 421, 633
BssECI CCNNGG 1 cut(s) 619
BssMI GATC 4 cut(s) 259, 301, 437, 706
BssNI GRCGYC 3 cut(s) 213, 267, 273
Bst2UI CCWGG 1 cut(s) 19
Bst4CI ACNGT 1 cut(s) 139
Bst6I CTCTTC 1 cut(s) 645
BstACI GRCGYC 3 cut(s) 213, 267, 273
BstC8I GCNNGC 2 cut(s) 294, 586
BstDEI CTNAG 1 cut(s) 476
BstDSI CCRYGG 1 cut(s) 619
BstEII GGTNACC 1 cut(s) 21
BstF5I GGATG 2 cut(s) 652, 710
BstFNI CGCG 1 cut(s) 149
BstH2I RGCGCY 1 cut(s) 270
BstHHI GCGC 2 cut(s) 151, 269
BstKTI GATC 4 cut(s) 262, 304, 440, 709
BstMAI GTCTC 2 cut(s) 415, 667
BstMBI GATC 4 cut(s) 259, 301, 437, 706
BstMCI CGRYCG 1 cut(s) 262
BstNI CCWGG 1 cut(s) 19
BstPI GGTNACC 1 cut(s) 21
BstSCI CCNGG 1 cut(s) 17
BstSLI GKGCMC 1 cut(s) 484
BstUI CGCG 1 cut(s) 149
BsuRI GGCC 3 cut(s) 482, 502, 588
BtgI CCRYGG 1 cut(s) 619
BtsCI GGATG 2 cut(s) 652, 710
Cac8I GCNNGC 2 cut(s) 294, 586
CfoI GCGC 2 cut(s) 151, 269
Cfr13I GGNCC 3 cut(s) 286, 480, 481
CseI GACGC 2 cut(s) 221, 281
CspCI CAANNNNNGTGG 4 cut(s) 447, 482, 534, 569
DdeI CTNAG 1 cut(s) 476
DinI GGCGCC 1 cut(s) 268
DpnI GATC 4 cut(s) 261, 303, 439, 708
DpnII GATC 4 cut(s) 259, 301, 437, 706
EaeI YGGCCR 1 cut(s) 500
Eam1104I CTCTTC 1 cut(s) 645
EarI CTCTTC 1 cut(s) 645
Eco147I AGGCCT 1 cut(s) 588
Eco24I GRGCYC 2 cut(s) 484, 619
Eco31I GGTCTC 1 cut(s) 415
Eco47I GGWCC 1 cut(s) 286
Eco91I GGTNACC 1 cut(s) 21
EcoO109I RGGNCCY 1 cut(s) 480
EcoO65I GGTNACC 1 cut(s) 21
EcoRII CCWGG 1 cut(s) 17
EcoT38I GRGCYC 2 cut(s) 484, 619
EgeI GGCGCC 1 cut(s) 268
EheI GGCGCC 1 cut(s) 268
FaiI YATR 3 cut(s) 390, 491, 640
FauI CCCGC 3 cut(s) 93, 140, 154
FbaI TGATCA 1 cut(s) 301
FblI GTMKAC 1 cut(s) 361
FokI GGATG 2 cut(s) 659, 697
FriOI GRGCYC 2 cut(s) 484, 619
FspBI CTAG 2 cut(s) 68, 293
GlaI GCGC 2 cut(s) 150, 268
GsuI CTGGAG 2 cut(s) 264, 318
HaeII RGCGCY 1 cut(s) 270
HaeIII GGCC 3 cut(s) 482, 502, 588
HgaI GACGC 2 cut(s) 221, 281
HhaI GCGC 2 cut(s) 151, 269
Hin1I GRCGYC 3 cut(s) 213, 267, 273
Hin6I GCGC 2 cut(s) 149, 267
HinP1I GCGC 2 cut(s) 149, 267
HinfI GANTC 1 cut(s) 494
HphI GGTGA 4 cut(s) 15, 48, 82, 635
Hpy166II GTNNAC 4 cut(s) 362, 448, 469, 626
Hpy188I TCNGA 3 cut(s) 306, 540, 731
Hpy188III TCNNGA 6 cut(s) 239, 281, 441, 577, 666, 694
Hpy8I GTNNAC 4 cut(s) 362, 448, 469, 626
Hpy99I CGWCG 1 cut(s) 275
HpyAV CCTTC 3 cut(s) 112, 117, 190
HpyCH4III ACNGT 1 cut(s) 139
HpyCH4V TGCA 2 cut(s) 545, 584
HpyF3I CTNAG 1 cut(s) 476
Hsp92I GRCGYC 3 cut(s) 213, 267, 273
HspAI GCGC 2 cut(s) 149, 267
KasI GGCGCC 1 cut(s) 266
Ksp22I TGATCA 1 cut(s) 301
Kzo9I GATC 4 cut(s) 259, 301, 437, 706
LmnI GCTCC 4 cut(s) 36, 232, 343, 379
LweI GCATC 1 cut(s) 232
MaeI CTAG 2 cut(s) 68, 293
MaeIII GTNAC 3 cut(s) 21, 328, 517
MalI GATC 4 cut(s) 261, 303, 439, 708
MbiI CCGCTC 1 cut(s) 88
MboI GATC 4 cut(s) 259, 301, 437, 706
MboII GAAGA 3 cut(s) 3, 6, 662
MhlI GDGCHC 2 cut(s) 484, 619
MlsI TGGCCA 1 cut(s) 502
MluCI AATT 4 cut(s) 174, 563, 633, 698
MluNI TGGCCA 1 cut(s) 502
Mly113I GGCGCC 1 cut(s) 267
MlyI GAGTC 1 cut(s) 503
MnlI CCTC 8 cut(s) 64, 69, 103, 223, 257, 277, 546, 662
Mox20I TGGCCA 1 cut(s) 502
MscI TGGCCA 1 cut(s) 502
MseI TTAA 3 cut(s) 434, 530, 600
Msp20I TGGCCA 1 cut(s) 502
MspR9I CCNGG 1 cut(s) 19
Mva1269I GAATGC 1 cut(s) 547
MvaI CCWGG 1 cut(s) 19
MvnI CGCG 1 cut(s) 149
NarI GGCGCC 1 cut(s) 267
NdeII GATC 4 cut(s) 259, 301, 437, 706
NheI GCTAGC 1 cut(s) 292
NlaIV GGNNCC 4 cut(s) 268, 345, 482, 606
NmuCI GTSAC 1 cut(s) 21
PceI AGGCCT 1 cut(s) 588
PctI GAATGC 1 cut(s) 547
PflMI CCANNNNNTGG 1 cut(s) 490
Ple19I CGATCG 1 cut(s) 262
PleI GAGTC 1 cut(s) 502
PluTI GGCGCC 1 cut(s) 270
PpsI GAGTC 1 cut(s) 502
Psp6I CCWGG 1 cut(s) 17
PspEI GGTNACC 1 cut(s) 21
PspGI CCWGG 1 cut(s) 17
PspN4I GGNNCC 4 cut(s) 268, 345, 482, 606
PspOMI GGGCCC 1 cut(s) 480
PspPI GGNCC 3 cut(s) 286, 480, 481
PvuI CGATCG 1 cut(s) 262
SaqAI TTAA 3 cut(s) 434, 530, 600
Sau3AI GATC 4 cut(s) 259, 301, 437, 706
Sau96I GGNCC 3 cut(s) 286, 480, 481
SchI GAGTC 1 cut(s) 503
ScrFI CCNGG 1 cut(s) 19
SduI GDGCHC 2 cut(s) 484, 619
SfaNI GCATC 1 cut(s) 232
SfoI GGCGCC 1 cut(s) 268
SinI GGWCC 1 cut(s) 286
SmlI CTYRAG 2 cut(s) 224, 664
SmoI CTYRAG 2 cut(s) 224, 664
SpeI ACTAGT 1 cut(s) 67
Sse9I AATT 4 cut(s) 174, 563, 633, 698
SseBI AGGCCT 1 cut(s) 588
SsiI CCGC 3 cut(s) 86, 133, 147
SspDI GGCGCC 1 cut(s) 266
SspMI CTAG 2 cut(s) 68, 293
StuI AGGCCT 1 cut(s) 588
StyD4I CCNGG 1 cut(s) 17
TaaI ACNGT 1 cut(s) 139
TaqI TCGA 6 cut(s) 240, 258, 262, 366, 567, 718
TasI AATT 4 cut(s) 174, 563, 633, 698
Tru1I TTAA 3 cut(s) 434, 530, 600
Tru9I TTAA 3 cut(s) 434, 530, 600
TseFI GTSAC 1 cut(s) 21
Tsp45I GTSAC 1 cut(s) 21
TspDTI ATGAA 2 cut(s) 663, 679
Van91I CCANNNNNTGG 1 cut(s) 490
VpaK11BI GGWCC 1 cut(s) 286
XapI RAATTY 2 cut(s) 174, 563
XmiI GTMKAC 1 cut(s) 361
XspI CTAG 2 cut(s) 68, 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.