RLG00000002013

plastid-lipid-associated protein 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0005370
Physical Location & Seq
Reverse (-)
24338702 .. 24341931
3230 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002013

Sequence Viewer

Length: 795 bp
ATGAACCTGAGCATGGCATTATCTTCTTCTTGGTCGCCTACGCTCCTTACAACCACCAAATCCTCCTCACCTCCCAAACTTGTCCATTCTCATTCCCCGCTCACCTCCTTGACCTTCCCTTCCAACCCCACCACCACCACCCACTCCATTGTTCCCGCGCTTTCCCACAAATGGAGAGCCAGAATTTCCTTCTTTCCTGCGTTTTCGAGCAAAGACAAAGACGCCAAAACTCTCAAGGAGGAGCTTCTCGATGCCATTGCTTCGCTCGATCGAGGCGCCGACGCCACTCCAGAGGACCAGCAAGCTGTTGATCAGATTGCACGCAAACTTGAAGCAGTTAGTCCAGTAAAGGAGCCACTCAAATCTGGTCTACTCGATGGAAAATATGAGCTTCTCTACACTACTTCAAAATCTCTTCTCCAAACTGGGAGACCCAAGTTTTTAAGATCGAGAGTAAACTACCAAGCAATCAATGTGGACACTCTCAGGGCCCAAAATATGGAGTCGTGGCCAACTTTCAATCAGGTAACAGCCGATTTAATACCTCTTAATGCAAGAAGAGTGGCTGTAAAGTTCGACTATTTCAAGATTGCAGGCCTGATACCTGTTAAGGCACCTGACAGAGCCCCTGGTGAACTGGAAATTACGTATTTAGATGAAGAGTTGCGAATTTCTGTTTCATCTAATTTGCTGACAACTTGTTCGGTCATTACTATTGACAGTGTCTCAAGAGGTGATAAAGGAAACCTGTTCATCTTGAAATTGGTTGATCCATCCTATCGAGTTCCTGTCTGA

Protein Analysis

265

Amino Acids

29.14

Weight (kDa)

9.21

Isoelectric Point (pI)

38.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP_fibrillin PF04755 77 - 225 3.9e-28 PAP_fibrillin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 275, 613
AccB7I CCANNNNNTGG 1 cut(s) 499
AccBSI CCGCTC 1 cut(s) 100
AccI GTMKAC 1 cut(s) 370
AccII CGCG 1 cut(s) 158
AciI CCGC 2 cut(s) 98, 156
AclWI GGATC 1 cut(s) 764
AcoI YGGCCR 1 cut(s) 509
AcsI RAATTY 2 cut(s) 183, 669
AcyI GRCGYC 3 cut(s) 222, 276, 282
AfiI CCNNNNNNNGG 3 cut(s) 13, 171, 499
AgsI TTSAA 5 cut(s) 332, 408, 520, 586, 760
AjnI CCWGG 1 cut(s) 628
AluBI AGCT 3 cut(s) 244, 305, 391
AluI AGCT 3 cut(s) 244, 305, 391
Alw26I GTCTC 2 cut(s) 424, 730
AlwI GGATC 1 cut(s) 764
AoxI GGCC 3 cut(s) 489, 509, 595
ApaI GGGCCC 1 cut(s) 493
ApoI RAATTY 2 cut(s) 183, 669
ArsI GACNNNNNNTTYG 2 cut(s) 685, 717
AspLEI GCGC 2 cut(s) 160, 278
AspS9I GGNCC 3 cut(s) 295, 489, 490
AsuHPI GGTGA 4 cut(s) 60, 94, 644, 746
AvaII GGWCC 1 cut(s) 295
BaeGI GKGCMC 1 cut(s) 493
BalI TGGCCA 1 cut(s) 511
BanI GGYRCC 2 cut(s) 275, 613
BanII GRGCYC 2 cut(s) 493, 628
BccI CCATC 2 cut(s) 371, 781
BcgI CGANNNNNNTGC 2 cut(s) 239, 273
BciT130I CCWGG 1 cut(s) 630
BclI TGATCA 1 cut(s) 310
BcoDI GTCTC 2 cut(s) 424, 730
BfoI RGCGCY 1 cut(s) 279
Bme1390I CCNGG 1 cut(s) 630
Bme18I GGWCC 1 cut(s) 295
BmgT120I GGNCC 3 cut(s) 295, 489, 490
BmiI GGNNCC 4 cut(s) 277, 354, 491, 615
BmrFI CCNGG 1 cut(s) 630
BmrI ACTGGG 1 cut(s) 435
BmsI GCATC 1 cut(s) 241
BmuI ACTGGG 1 cut(s) 435
BpmI CTGGAG 1 cut(s) 273
Bpu10I CCTNAGC 1 cut(s) 8
BpuEI CTTGAG 2 cut(s) 218, 712
BsaAI YACGTR 1 cut(s) 648
BsaHI GRCGYC 3 cut(s) 222, 276, 282
BsaI GGTCTC 1 cut(s) 424
BsaJI CCNNGG 1 cut(s) 628
Bsc4I CCNNNNNNNGG 3 cut(s) 13, 171, 499
Bse1I ACTGG 3 cut(s) 344, 430, 642
Bse3DI GCAATG 1 cut(s) 255
BseBI CCWGG 1 cut(s) 630
BseDI CCNNGG 1 cut(s) 628
BseGI GGATG 1 cut(s) 773
BseLI CCNNNNNNNGG 3 cut(s) 13, 171, 499
BseMI GCAATG 1 cut(s) 255
BseMII CTCAG 1 cut(s) 499
BseNI ACTGG 3 cut(s) 344, 430, 642
BseRI GAGGAG 2 cut(s) 55, 254
BseSI GKGCMC 1 cut(s) 493
Bsh1236I CGCG 1 cut(s) 158
Bsh1285I CGRYCG 1 cut(s) 271
BshFI GGCC 3 cut(s) 491, 511, 597
BshNI GGYRCC 2 cut(s) 275, 613
BsiEI CGRYCG 1 cut(s) 271
BslI CCNNNNNNNGG 3 cut(s) 13, 171, 499
BsmAI GTCTC 2 cut(s) 424, 730
BsnI GGCC 3 cut(s) 491, 511, 597
Bso31I GGTCTC 1 cut(s) 424
Bsp120I GGGCCC 1 cut(s) 489
Bsp1286I GDGCHC 2 cut(s) 493, 628
Bsp143I GATC 4 cut(s) 268, 310, 446, 769
BspACI CCGC 2 cut(s) 98, 156
BspANI GGCC 3 cut(s) 491, 511, 597
BspCNI CTCAG 1 cut(s) 498
BspFNI CGCG 1 cut(s) 158
BspLI GGNNCC 4 cut(s) 277, 354, 491, 615
BspPI GGATC 1 cut(s) 764
BspT107I GGYRCC 2 cut(s) 275, 613
BspTNI GGTCTC 1 cut(s) 424
BsrBI CCGCTC 1 cut(s) 100
BsrDI GCAATG 1 cut(s) 255
BsrI ACTGG 3 cut(s) 344, 430, 642
BssECI CCNNGG 1 cut(s) 628
BssMI GATC 4 cut(s) 268, 310, 446, 769
BssNI GRCGYC 3 cut(s) 222, 276, 282
Bst2UI CCWGG 1 cut(s) 630
Bst4CI ACNGT 1 cut(s) 722
Bst6I CTCTTC 3 cut(s) 420, 553, 654
BstACI GRCGYC 3 cut(s) 222, 276, 282
BstBAI YACGTR 1 cut(s) 648
BstC8I GCNNGC 3 cut(s) 303, 322, 595
BstDEI CTNAG 2 cut(s) 8, 485
BstF5I GGATG 1 cut(s) 773
BstFNI CGCG 1 cut(s) 158
BstH2I RGCGCY 1 cut(s) 279
BstHHI GCGC 2 cut(s) 160, 278
BstKTI GATC 4 cut(s) 271, 313, 449, 772
BstMAI GTCTC 2 cut(s) 424, 730
BstMBI GATC 4 cut(s) 268, 310, 446, 769
BstMCI CGRYCG 1 cut(s) 271
BstNI CCWGG 1 cut(s) 630
BstSCI CCNGG 1 cut(s) 628
BstSLI GKGCMC 1 cut(s) 493
BstSNI TACGTA 1 cut(s) 648
BstUI CGCG 1 cut(s) 158
BsuRI GGCC 3 cut(s) 491, 511, 597
BtsCI GGATG 1 cut(s) 773
BtsIMutI CAGTG 1 cut(s) 727
Cac8I GCNNGC 3 cut(s) 303, 322, 595
CfoI GCGC 2 cut(s) 160, 278
Cfr13I GGNCC 3 cut(s) 295, 489, 490
CseI GACGC 2 cut(s) 230, 290
CspCI CAANNNNNGTGG 4 cut(s) 456, 491, 543, 578
CviAII CATG 1 cut(s) 13
DdeI CTNAG 2 cut(s) 8, 485
DinI GGCGCC 1 cut(s) 277
DpnI GATC 4 cut(s) 270, 312, 448, 771
DpnII GATC 4 cut(s) 268, 310, 446, 769
EaeI YGGCCR 1 cut(s) 509
Eam1104I CTCTTC 3 cut(s) 420, 553, 654
EarI CTCTTC 3 cut(s) 420, 553, 654
Eco105I TACGTA 1 cut(s) 648
Eco147I AGGCCT 1 cut(s) 597
Eco24I GRGCYC 2 cut(s) 493, 628
Eco31I GGTCTC 1 cut(s) 424
Eco47I GGWCC 1 cut(s) 295
EcoO109I RGGNCCY 1 cut(s) 489
EcoRII CCWGG 1 cut(s) 628
EcoT38I GRGCYC 2 cut(s) 493, 628
EgeI GGCGCC 1 cut(s) 277
EheI GGCGCC 1 cut(s) 277
FaeI CATG 1 cut(s) 16
FaiI YATR 3 cut(s) 14, 387, 500
FatI CATG 1 cut(s) 12
FauI CCCGC 2 cut(s) 105, 163
FbaI TGATCA 1 cut(s) 310
FblI GTMKAC 1 cut(s) 370
FokI GGATG 1 cut(s) 760
FriOI GRGCYC 2 cut(s) 493, 628
GlaI GCGC 2 cut(s) 159, 277
GsuI CTGGAG 1 cut(s) 273
HaeII RGCGCY 1 cut(s) 279
HaeIII GGCC 3 cut(s) 491, 511, 597
HgaI GACGC 2 cut(s) 230, 290
HhaI GCGC 2 cut(s) 160, 278
Hin1I GRCGYC 3 cut(s) 222, 276, 282
Hin1II CATG 1 cut(s) 16
Hin6I GCGC 2 cut(s) 158, 276
HinP1I GCGC 2 cut(s) 158, 276
HinfI GANTC 1 cut(s) 503
HphI GGTGA 4 cut(s) 60, 94, 644, 746
Hpy166II GTNNAC 4 cut(s) 371, 457, 478, 635
Hpy188I TCNGA 2 cut(s) 315, 794
Hpy188III TCNNGA 6 cut(s) 248, 290, 450, 586, 729, 757
Hpy8I GTNNAC 4 cut(s) 371, 457, 478, 635
Hpy99I CGWCG 1 cut(s) 284
HpyAV CCTTC 3 cut(s) 124, 129, 199
HpyCH4III ACNGT 1 cut(s) 722
HpyCH4IV ACGT 1 cut(s) 647
HpyCH4V TGCA 3 cut(s) 320, 554, 593
HpyF3I CTNAG 2 cut(s) 8, 485
HpySE526I ACGT 1 cut(s) 647
Hsp92I GRCGYC 3 cut(s) 222, 276, 282
Hsp92II CATG 1 cut(s) 16
HspAI GCGC 2 cut(s) 158, 276
KasI GGCGCC 1 cut(s) 275
Ksp22I TGATCA 1 cut(s) 310
Kzo9I GATC 4 cut(s) 268, 310, 446, 769
LmnI GCTCC 3 cut(s) 48, 241, 352
LweI GCATC 1 cut(s) 241
MaeII ACGT 1 cut(s) 647
MaeIII GTNAC 1 cut(s) 526
MalI GATC 4 cut(s) 270, 312, 448, 771
MbiI CCGCTC 1 cut(s) 100
MboI GATC 4 cut(s) 268, 310, 446, 769
MboII GAAGA 5 cut(s) 15, 18, 407, 570, 671
MhlI GDGCHC 2 cut(s) 493, 628
MlsI TGGCCA 1 cut(s) 511
MluCI AATT 5 cut(s) 183, 642, 669, 685, 761
MluNI TGGCCA 1 cut(s) 511
Mly113I GGCGCC 1 cut(s) 276
MlyI GAGTC 1 cut(s) 512
MmeI TCCRAC 1 cut(s) 147
MnlI CCTC 9 cut(s) 73, 76, 81, 115, 232, 266, 286, 555, 725
Mox20I TGGCCA 1 cut(s) 511
MscI TGGCCA 1 cut(s) 511
MseI TTAA 4 cut(s) 443, 539, 549, 609
Msp20I TGGCCA 1 cut(s) 511
MspR9I CCNGG 1 cut(s) 630
MvaI CCWGG 1 cut(s) 630
MvnI CGCG 1 cut(s) 158
NarI GGCGCC 1 cut(s) 276
NdeII GATC 4 cut(s) 268, 310, 446, 769
NlaIII CATG 1 cut(s) 16
NlaIV GGNNCC 4 cut(s) 277, 354, 491, 615
PceI AGGCCT 1 cut(s) 597
PflFI GACNNNGTC 1 cut(s) 722
PflMI CCANNNNNTGG 1 cut(s) 499
Ple19I CGATCG 1 cut(s) 271
PleI GAGTC 1 cut(s) 511
PluTI GGCGCC 1 cut(s) 279
PpsI GAGTC 1 cut(s) 511
Ppu21I YACGTR 1 cut(s) 648
Psp6I CCWGG 1 cut(s) 628
PspGI CCWGG 1 cut(s) 628
PspN4I GGNNCC 4 cut(s) 277, 354, 491, 615
PspOMI GGGCCC 1 cut(s) 489
PspPI GGNCC 3 cut(s) 295, 489, 490
PsyI GACNNNGTC 1 cut(s) 722
PvuI CGATCG 1 cut(s) 271
SaqAI TTAA 4 cut(s) 443, 539, 549, 609
Sau3AI GATC 4 cut(s) 268, 310, 446, 769
Sau96I GGNCC 3 cut(s) 295, 489, 490
SchI GAGTC 1 cut(s) 512
ScrFI CCNGG 1 cut(s) 630
SduI GDGCHC 2 cut(s) 493, 628
SfaNI GCATC 1 cut(s) 241
SfoI GGCGCC 1 cut(s) 277
SinI GGWCC 1 cut(s) 295
SmlI CTYRAG 2 cut(s) 233, 727
SmoI CTYRAG 2 cut(s) 233, 727
SnaBI TACGTA 1 cut(s) 648
Sse9I AATT 5 cut(s) 183, 642, 669, 685, 761
SseBI AGGCCT 1 cut(s) 597
SsiI CCGC 2 cut(s) 98, 156
SspDI GGCGCC 1 cut(s) 275
StuI AGGCCT 1 cut(s) 597
StyD4I CCNGG 1 cut(s) 628
TaaI ACNGT 1 cut(s) 722
TaiI ACGT 1 cut(s) 650
TaqI TCGA 8 cut(s) 206, 249, 267, 271, 375, 449, 576, 781
TaqII GACCGA 1 cut(s) 694
TasI AATT 5 cut(s) 183, 642, 669, 685, 761
Tru1I TTAA 4 cut(s) 443, 539, 549, 609
Tru9I TTAA 4 cut(s) 443, 539, 549, 609
TscAI CASTG 1 cut(s) 727
TspDTI ATGAA 4 cut(s) 17, 669, 672, 742
TspRI CASTG 1 cut(s) 727
Tth111I GACNNNGTC 1 cut(s) 722
Van91I CCANNNNNTGG 1 cut(s) 499
VpaK11BI GGWCC 1 cut(s) 295
XapI RAATTY 2 cut(s) 183, 669
XmiI GTMKAC 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.