FvH4_5g30150

Basic blue

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0003671
Physical Location & Seq
Reverse (-)
21055790 .. 21056837
1048 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g30150.t1

Sequence Viewer

Length: 372 bp
ATGGCTCAAGCTAGAGTCAGTGCAAGAATTAATGTGATGTTGTTAGTGTTTTGTGTGTTGATTCACTACAAGATTGCTAGTGCGACTACCTACACTGTTGGAGATGCAGCAGGATGGACATTTGGAATACAAAGTTGGCCAACAGGGAAGCAATTTCATGCTGGAGATATTTTAGTTTTCAAGTACAACAAGAATGTACATAATGTAATTGTGACTGATGAAAATGGATTCAAGGCATGCAATACTGCAACAGGTCAAAAATTTATATCAGGCAATGATCAGATCAAACTTAAAAAAGGACAAAACTATTTCATCTGCAGTACTCCTGGGCATTGTGCTGGTGGAATGAAAATGGCAATATCTGCTGCATAG

Protein Analysis

124

Amino Acids

13.32

Weight (kDa)

9.45

Isoelectric Point (pI)

20.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 39 - 116 6.7e-25 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 137
AcsI RAATTY 1 cut(s) 260
AfaI GTAC 3 cut(s) 185, 198, 322
AgsI TTSAA 2 cut(s) 181, 232
AjnI CCWGG 1 cut(s) 325
AjuI GAANNNNNNNTTGG 2 cut(s) 118, 150
AluBI AGCT 1 cut(s) 11
AluI AGCT 1 cut(s) 11
AoxI GGCC 1 cut(s) 137
ApeKI GCWGC 2 cut(s) 107, 365
ApoI RAATTY 1 cut(s) 260
AseI ATTAAT 1 cut(s) 30
BalI TGGCCA 1 cut(s) 139
BbvI GCAGC 2 cut(s) 119, 352
BccI CCATC 1 cut(s) 108
BciT130I CCWGG 1 cut(s) 327
BclI TGATCA 1 cut(s) 277
BfaI CTAG 2 cut(s) 12, 78
BfmI CTRYAG 1 cut(s) 316
BisI GCNGC 2 cut(s) 108, 366
BlsI GCNGC 2 cut(s) 109, 367
BmcAI AGTACT 1 cut(s) 322
Bme1390I CCNGG 1 cut(s) 327
BmrFI CCNGG 1 cut(s) 327
BmsI GCATC 1 cut(s) 94
BpmI CTGGAG 1 cut(s) 183
BsaJI CCNNGG 1 cut(s) 326
Bse3DI GCAATG 1 cut(s) 280
BseBI CCWGG 1 cut(s) 327
BseDI CCNNGG 1 cut(s) 326
BseGI GGATG 1 cut(s) 119
BseMI GCAATG 1 cut(s) 280
BseXI GCAGC 2 cut(s) 119, 352
BshFI GGCC 1 cut(s) 139
BsnI GGCC 1 cut(s) 139
Bsp1407I TGTACA 1 cut(s) 196
Bsp143I GATC 2 cut(s) 277, 282
BspANI GGCC 1 cut(s) 139
BspMAI CTGCAG 1 cut(s) 320
BsrDI GCAATG 1 cut(s) 280
BsrGI TGTACA 1 cut(s) 196
BssECI CCNNGG 1 cut(s) 326
BssMI GATC 2 cut(s) 277, 282
Bst2UI CCWGG 1 cut(s) 327
Bst4CI ACNGT 1 cut(s) 97
BstAPI GCANNNNNTGC 1 cut(s) 362
BstAUI TGTACA 1 cut(s) 196
BstC8I GCNNGC 1 cut(s) 238
BstF5I GGATG 1 cut(s) 119
BstKTI GATC 2 cut(s) 280, 285
BstMBI GATC 2 cut(s) 277, 282
BstMWI GCNNNNNNNGC 1 cut(s) 362
BstNI CCWGG 1 cut(s) 327
BstNSI RCATGY 1 cut(s) 240
BstSCI CCNGG 1 cut(s) 325
BstSFI CTRYAG 1 cut(s) 316
BstV1I GCAGC 2 cut(s) 119, 352
BsuRI GGCC 1 cut(s) 139
BtsCI GGATG 1 cut(s) 119
BtsIMutI CAGTG 2 cut(s) 25, 93
Cac8I GCNNGC 1 cut(s) 238
Csp6I GTAC 3 cut(s) 184, 197, 321
CviAII CATG 2 cut(s) 158, 237
CviJI RGCY 3 cut(s) 5, 11, 139
CviKI_1 RGCY 3 cut(s) 5, 11, 139
CviQI GTAC 3 cut(s) 184, 197, 321
DpnI GATC 2 cut(s) 279, 284
DpnII GATC 2 cut(s) 277, 282
EaeI YGGCCR 1 cut(s) 137
EcoRII CCWGG 1 cut(s) 325
FaeI CATG 2 cut(s) 161, 240
FaiI YATR 5 cut(s) 159, 201, 238, 266, 370
FatI CATG 2 cut(s) 157, 236
FbaI TGATCA 1 cut(s) 277
Fnu4HI GCNGC 2 cut(s) 108, 366
FokI GGATG 1 cut(s) 126
Fsp4HI GCNGC 2 cut(s) 108, 366
FspBI CTAG 2 cut(s) 12, 78
GluI GCNGC 2 cut(s) 108, 366
GsuI CTGGAG 1 cut(s) 183
HaeIII GGCC 1 cut(s) 139
Hin1II CATG 2 cut(s) 161, 240
HinfI GANTC 3 cut(s) 15, 61, 228
Hpy188I TCNGA 1 cut(s) 282
HpyCH4III ACNGT 1 cut(s) 97
HpyCH4V TGCA 6 cut(s) 23, 107, 240, 248, 318, 368
HpyF10VI GCNNNNNNNGC 1 cut(s) 362
Hsp92II CATG 2 cut(s) 161, 240
Ksp22I TGATCA 1 cut(s) 277
Kzo9I GATC 2 cut(s) 277, 282
LpnPI CCDG 8 cut(s) 96, 129, 147, 237, 255, 312, 324, 339
Lsp1109I GCAGC 2 cut(s) 119, 352
LweI GCATC 1 cut(s) 94
MaeI CTAG 2 cut(s) 12, 78
MaeIII GTNAC 1 cut(s) 211
MalI GATC 2 cut(s) 279, 284
MboI GATC 2 cut(s) 277, 282
MlsI TGGCCA 1 cut(s) 139
MluCI AATT 4 cut(s) 27, 152, 207, 260
MluNI TGGCCA 1 cut(s) 139
MlyI GAGTC 1 cut(s) 24
MmeI TCCRAC 1 cut(s) 79
Mox20I TGGCCA 1 cut(s) 139
MscI TGGCCA 1 cut(s) 139
MseI TTAA 2 cut(s) 30, 291
Msp20I TGGCCA 1 cut(s) 139
MspR9I CCNGG 1 cut(s) 327
MvaI CCWGG 1 cut(s) 327
MwoI GCNNNNNNNGC 1 cut(s) 362
NdeII GATC 2 cut(s) 277, 282
NlaIII CATG 2 cut(s) 161, 240
NmuCI GTSAC 1 cut(s) 211
NspI RCATGY 1 cut(s) 240
PaeI GCATGC 1 cut(s) 240
PfeI GAWTC 2 cut(s) 61, 228
PkrI GCNGC 2 cut(s) 109, 367
PleI GAGTC 1 cut(s) 23
PpsI GAGTC 1 cut(s) 23
PshBI ATTAAT 1 cut(s) 30
Psp6I CCWGG 1 cut(s) 325
PspGI CCWGG 1 cut(s) 325
PstI CTGCAG 1 cut(s) 320
RsaI GTAC 3 cut(s) 185, 198, 322
RsaNI GTAC 3 cut(s) 184, 197, 321
SaqAI TTAA 2 cut(s) 30, 291
SatI GCNGC 2 cut(s) 108, 366
Sau3AI GATC 2 cut(s) 277, 282
ScaI AGTACT 1 cut(s) 322
SchI GAGTC 1 cut(s) 24
ScrFI CCNGG 1 cut(s) 327
SetI ASST 3 cut(s) 13, 92, 256
SfaNI GCATC 1 cut(s) 94
SfcI CTRYAG 1 cut(s) 316
SmlI CTYRAG 1 cut(s) 6
SmoI CTYRAG 1 cut(s) 6
SphI GCATGC 1 cut(s) 240
Sse9I AATT 4 cut(s) 27, 152, 207, 260
SspMI CTAG 2 cut(s) 12, 78
StyD4I CCNGG 1 cut(s) 325
TaaI ACNGT 1 cut(s) 97
TasI AATT 4 cut(s) 27, 152, 207, 260
TatI WGTACW 3 cut(s) 183, 196, 320
TfiI GAWTC 2 cut(s) 61, 228
Tru1I TTAA 2 cut(s) 30, 291
Tru9I TTAA 2 cut(s) 30, 291
TscAI CASTG 2 cut(s) 25, 100
TseFI GTSAC 1 cut(s) 211
TseI GCWGC 2 cut(s) 107, 365
Tsp45I GTSAC 1 cut(s) 211
TspDTI ATGAA 4 cut(s) 146, 234, 301, 362
TspRI CASTG 2 cut(s) 25, 100
VspI ATTAAT 1 cut(s) 30
XapI RAATTY 1 cut(s) 260
XceI RCATGY 1 cut(s) 240
XspI CTAG 2 cut(s) 12, 78
ZrmI AGTACT 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.