Rorug07G0223200

Basic blue protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0003671
Physical Location & Seq
Reverse (-)
20276300 .. 20279387
3088 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0223200.1

Sequence Viewer

Length: 1056 bp
ATGGCAGTTCAAGCTCAGTACCCTTCCAATATTCTATTCCTAAACAGAAACGCACAAGAACCGCATGATTATTCATTACAAGCGCAACAAGGATTGTACCTTGATCAATCCCACATGCTATTCAACAATGGTGTAGGAGGGAATAGTACTAATCAGCGCAAGAGAGGAAGAGAAACTTCAGTTGGCACTGATATCACACCTTCAATGATTCCCTTCTCTTTGCAACAGTCCCAACCTCCTCAACTCATCGACCTCTCTCAGCTCCACAATCACAATCACAATCACAATCACAACCACAACCACCATCCCAATGTCGTCTCCACCGGCCTCCGATTATCTTTTGGAGACCAACAACAACATCAGCAACAATTGCAACAGCATCATCACAATCAACAACAACAACAACAACAGGATTGTCACTCTTCTTCATCTCTGTTCTCTGTTTTGGCAGAGGACTTTGCCTCCCAAATCAAACAACAGAGGAACGAATTAGACCAGTTCCTTCAAGCCCAGGCGGAACGACTACAGCGCACATTAGCAGAGAAGCGGCAGACACACTACCGTGCGCTACTGGGCGCAGCTGAGGAGTCTATTTCCCGTAAATTGAGAGAGAAGGATGCAGAGGTCCAAAAGGCCACGCGCAGGAACGCCGAGTTAGAGGCACGCGCCGCCCAGCTCAGCGTTGAGGCGCAGATATGGCAGGCCAAGGCGCGTGCGCAGGAGGCCACAGCCGCGTCCCTCCAGGTCCAAGTCCAACAGGCCAAGGCCATGATGAGCGCCGGGTTCCAAGTGGCGGGTGCGCAGGATAGCAGGAGAGTCGACGATGGGCTAACATGCGCCGAAAACCAGGCGGAGGATGCCGAGTCAGGGTACATTGACCCGGAACGCGCCACCGGGTCGGGTCCTAGTTGTAAAGCGTGTCGGAAAGGAGTGGCGTCGGTGGTGCTGTTGCCGTGTCGGCACCTATGCCTCTGTACAGAGTGTGACCAAATGGTTCAGTCCTGCCCATTGTGCCTCACCTTGAGAAATTCGAGTGTGGAAGTCTTCCTTTGCTAG

Protein Analysis

351

Amino Acids

39.2

Weight (kDa)

6.62

Isoelectric Point (pI)

65.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 303 - 339 2.9e-09 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 717, 801
AccB1I GGYRCC 1 cut(s) 960
AccI GTMKAC 1 cut(s) 819
AccII CGCG 5 cut(s) 640, 666, 712, 734, 888
AciI CCGC 7 cut(s) 62, 515, 547, 669, 732, 794, 851
AcsI RAATTY 1 cut(s) 1027
AcuI CTGAAG 1 cut(s) 162
AcyI GRCGYC 1 cut(s) 935
AfaI GTAC 5 cut(s) 20, 98, 148, 872, 976
AfiI CCNNNNNNNGG 4 cut(s) 642, 793, 853, 867
AgsI TTSAA 4 cut(s) 11, 124, 204, 506
AjnI CCWGG 3 cut(s) 510, 741, 846
AjuI GAANNNNNNNTTGG 4 cut(s) 20, 52, 165, 197
AleI CACNNNNGTG 1 cut(s) 561
AluBI AGCT 4 cut(s) 14, 262, 581, 676
AluI AGCT 4 cut(s) 14, 262, 581, 676
Alw26I GTCTC 2 cut(s) 322, 339
AoxI GGCC 6 cut(s) 325, 633, 702, 723, 759, 765
ApeKI GCWGC 1 cut(s) 578
ApoI RAATTY 1 cut(s) 1027
AspS9I GGNCC 3 cut(s) 625, 745, 902
AsuC2I CCSGG 3 cut(s) 781, 881, 895
AsuHPI GGTGA 1 cut(s) 1009
AvaII GGWCC 3 cut(s) 625, 745, 902
BanI GGYRCC 1 cut(s) 960
BbsI GAAGAC 1 cut(s) 1036
BbvCI CCTCAGC 1 cut(s) 582
BbvI GCAGC 1 cut(s) 590
BccI CCATC 2 cut(s) 312, 818
BceAI ACGGC 1 cut(s) 937
BcgI CGANNNNNNTGC 2 cut(s) 799, 833
BciT130I CCWGG 3 cut(s) 512, 743, 848
BclI TGATCA 1 cut(s) 103
BcnI CCSGG 3 cut(s) 781, 881, 895
BcoDI GTCTC 2 cut(s) 322, 339
BfaI CTAG 2 cut(s) 906, 1054
BfmI CTRYAG 1 cut(s) 524
BfoI RGCGCY 1 cut(s) 780
BglI GCCNNNNNGGC 1 cut(s) 958
BisI GCNGC 4 cut(s) 548, 579, 669, 732
BlpI GCTNAGC 1 cut(s) 677
BlsI GCNGC 4 cut(s) 549, 580, 670, 733
BmcAI AGTACT 1 cut(s) 148
Bme1390I CCNGG 6 cut(s) 512, 743, 781, 848, 881, 895
Bme18I GGWCC 3 cut(s) 625, 745, 902
BmgT120I GGNCC 3 cut(s) 625, 745, 902
BmiI GGNNCC 3 cut(s) 785, 903, 962
BmrFI CCNGG 6 cut(s) 512, 743, 781, 848, 881, 895
BmrI ACTGGG 1 cut(s) 581
BmsI GCATC 3 cut(s) 388, 607, 847
BmuI ACTGGG 1 cut(s) 581
BpiI GAAGAC 1 cut(s) 1036
BpmI CTGGAG 1 cut(s) 725
Bpu10I CCTNAGC 1 cut(s) 582
Bpu1102I GCTNAGC 1 cut(s) 677
BpuEI CTTGAG 1 cut(s) 1042
BpuMI CCSGG 3 cut(s) 781, 881, 895
BsaHI GRCGYC 1 cut(s) 935
BsaI GGTCTC 1 cut(s) 339
BsaJI CCNNGG 3 cut(s) 510, 705, 762
BsaXI ACNNNNNCTCC 2 cut(s) 446, 476
Bsc4I CCNNNNNNNGG 4 cut(s) 642, 793, 853, 867
Bse118I RCCGGY 1 cut(s) 323
Bse1I ACTGG 2 cut(s) 496, 576
BseBI CCWGG 3 cut(s) 512, 743, 848
BseDI CCNNGG 3 cut(s) 510, 705, 762
BseGI GGATG 3 cut(s) 304, 622, 862
BseLI CCNNNNNNNGG 4 cut(s) 642, 793, 853, 867
BseMII CTCAG 4 cut(s) 29, 272, 573, 691
BseNI ACTGG 2 cut(s) 496, 576
BseRI GAGGAG 2 cut(s) 228, 599
BseXI GCAGC 1 cut(s) 590
BseYI CCCAGC 1 cut(s) 672
Bsh1236I CGCG 5 cut(s) 640, 666, 712, 734, 888
BshFI GGCC 6 cut(s) 327, 635, 704, 725, 761, 767
BshNI GGYRCC 1 cut(s) 960
BsiSI CCGG 4 cut(s) 324, 780, 881, 894
BslFI GGGAC 2 cut(s) 214, 721
BslI CCNNNNNNNGG 4 cut(s) 642, 793, 853, 867
BsmAI GTCTC 2 cut(s) 322, 339
BsmBI CGTCTC 1 cut(s) 322
BsmFI GGGAC 2 cut(s) 214, 721
BsnI GGCC 6 cut(s) 327, 635, 704, 725, 761, 767
Bso31I GGTCTC 1 cut(s) 339
Bsp1407I TGTACA 1 cut(s) 974
Bsp143I GATC 1 cut(s) 103
Bsp1720I GCTNAGC 1 cut(s) 677
BspACI CCGC 7 cut(s) 62, 515, 547, 669, 732, 794, 851
BspANI GGCC 6 cut(s) 327, 635, 704, 725, 761, 767
BspCNI CTCAG 4 cut(s) 28, 271, 574, 690
BspFNI CGCG 5 cut(s) 640, 666, 712, 734, 888
BspLI GGNNCC 3 cut(s) 785, 903, 962
BspT107I GGYRCC 1 cut(s) 960
BspTNI GGTCTC 1 cut(s) 339
BsrFI RCCGGY 1 cut(s) 323
BsrGI TGTACA 1 cut(s) 974
BsrI ACTGG 2 cut(s) 496, 576
BssAI RCCGGY 1 cut(s) 323
BssECI CCNNGG 3 cut(s) 510, 705, 762
BssMI GATC 1 cut(s) 103
BssNI GRCGYC 1 cut(s) 935
BssT1I CCWWGG 2 cut(s) 705, 762
Bst2UI CCWGG 3 cut(s) 512, 743, 848
Bst4CI ACNGT 2 cut(s) 228, 563
Bst6I CTCTTC 2 cut(s) 163, 427
BstACI GRCGYC 1 cut(s) 935
BstAPI GCANNNNNTGC 1 cut(s) 370
BstAUI TGTACA 1 cut(s) 974
BstC8I GCNNGC 3 cut(s) 664, 702, 714
BstDEI CTNAG 4 cut(s) 15, 258, 582, 677
BstF5I GGATG 3 cut(s) 304, 622, 862
BstFNI CGCG 5 cut(s) 640, 666, 712, 734, 888
BstH2I RGCGCY 1 cut(s) 780
BstKTI GATC 1 cut(s) 106
BstMAI GTCTC 2 cut(s) 322, 339
BstMBI GATC 1 cut(s) 103
BstMWI GCNNNNNNNGC 9 cut(s) 11, 370, 668, 697, 722, 731, 857, 958, 1011
BstNI CCWGG 3 cut(s) 512, 743, 848
BstNSI RCATGY 2 cut(s) 118, 837
BstSCI CCNGG 6 cut(s) 510, 741, 779, 846, 879, 893
BstSFI CTRYAG 1 cut(s) 524
BstUI CGCG 5 cut(s) 640, 666, 712, 734, 888
BstV1I GCAGC 1 cut(s) 590
BstV2I GAAGAC 1 cut(s) 1036
BsuRI GGCC 6 cut(s) 327, 635, 704, 725, 761, 767
BtsCI GGATG 3 cut(s) 304, 622, 862
BtsIMutI CAGTG 1 cut(s) 186
Cac8I GCNNGC 3 cut(s) 664, 702, 714
Cfr10I RCCGGY 1 cut(s) 323
Cfr13I GGNCC 3 cut(s) 625, 745, 902
CseI GACGC 2 cut(s) 723, 924
Csp6I GTAC 5 cut(s) 19, 97, 147, 871, 975
CviAII CATG 4 cut(s) 65, 115, 769, 834
CviQI GTAC 5 cut(s) 19, 97, 147, 871, 975
DdeI CTNAG 4 cut(s) 15, 258, 582, 677
DpnI GATC 1 cut(s) 105
DpnII GATC 1 cut(s) 103
Eam1104I CTCTTC 2 cut(s) 163, 427
EarI CTCTTC 2 cut(s) 163, 427
EciI GGCGGA 2 cut(s) 530, 866
Eco130I CCWWGG 2 cut(s) 705, 762
Eco31I GGTCTC 1 cut(s) 339
Eco32I GATATC 1 cut(s) 193
Eco47I GGWCC 3 cut(s) 625, 745, 902
Eco57I CTGAAG 1 cut(s) 162
EcoO109I RGGNCCY 1 cut(s) 902
EcoRII CCWGG 3 cut(s) 510, 741, 846
EcoRV GATATC 1 cut(s) 193
EcoT14I CCWWGG 2 cut(s) 705, 762
ErhI CCWWGG 2 cut(s) 705, 762
Esp3I CGTCTC 1 cut(s) 322
FaeI CATG 4 cut(s) 68, 118, 772, 837
FaiI YATR 6 cut(s) 66, 116, 697, 770, 835, 967
FalI AAGNNNNNCTT 3 cut(s) 160, 192, 1032
FaqI GGGAC 2 cut(s) 214, 721
FatI CATG 4 cut(s) 64, 114, 768, 833
FauI CCCGC 1 cut(s) 787
FbaI TGATCA 1 cut(s) 103
FblI GTMKAC 1 cut(s) 819
Fnu4HI GCNGC 4 cut(s) 548, 579, 669, 732
FokI GGATG 3 cut(s) 291, 629, 869
Fsp4HI GCNGC 4 cut(s) 548, 579, 669, 732
FspBI CTAG 2 cut(s) 906, 1054
FspI TGCGCA 2 cut(s) 717, 801
GluI GCNGC 4 cut(s) 548, 579, 669, 732
GsaI CCCAGC 1 cut(s) 676
GsuI CTGGAG 1 cut(s) 725
HaeII RGCGCY 1 cut(s) 780
HaeIII GGCC 6 cut(s) 327, 635, 704, 725, 761, 767
HapII CCGG 4 cut(s) 324, 780, 881, 894
HgaI GACGC 2 cut(s) 723, 924
Hin1I GRCGYC 1 cut(s) 935
Hin1II CATG 4 cut(s) 68, 118, 772, 837
HincII GTYRAC 1 cut(s) 820
HindII GTYRAC 1 cut(s) 820
HinfI GANTC 4 cut(s) 208, 587, 816, 863
HpaII CCGG 4 cut(s) 324, 780, 881, 894
HphI GGTGA 1 cut(s) 1009
Hpy166II GTNNAC 1 cut(s) 820
Hpy188I TCNGA 2 cut(s) 332, 924
Hpy8I GTNNAC 1 cut(s) 820
Hpy99I CGWCG 2 cut(s) 824, 940
HpyAV CCTTC 5 cut(s) 33, 210, 223, 512, 607
HpyCH4III ACNGT 2 cut(s) 228, 563
HpyCH4V TGCA 3 cut(s) 223, 373, 620
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 370, 668, 697, 722, 731, 857, 958, 1011
HpyF3I CTNAG 4 cut(s) 15, 258, 582, 677
Hsp92I GRCGYC 1 cut(s) 935
Hsp92II CATG 4 cut(s) 68, 118, 772, 837
Ksp22I TGATCA 1 cut(s) 103
Kzo9I GATC 1 cut(s) 103
LmnI GCTCC 1 cut(s) 267
Lsp1109I GCAGC 1 cut(s) 590
LweI GCATC 3 cut(s) 388, 607, 847
MaeI CTAG 2 cut(s) 906, 1054
MaeIII GTNAC 2 cut(s) 416, 983
MalI GATC 1 cut(s) 105
MboI GATC 1 cut(s) 103
MboII GAAGA 4 cut(s) 180, 414, 417, 1036
MfeI CAATTG 1 cut(s) 368
MluCI AATT 4 cut(s) 368, 488, 602, 1027
MlyI GAGTC 3 cut(s) 596, 825, 872
MmeI TCCRAC 2 cut(s) 778, 902
MslI CAYNNNNRTG 2 cut(s) 309, 561
MspA1I CMGCKG 1 cut(s) 581
MspI CCGG 4 cut(s) 324, 780, 881, 894
MspR9I CCNGG 6 cut(s) 512, 743, 781, 848, 881, 895
MunI CAATTG 1 cut(s) 368
MvaI CCWGG 3 cut(s) 512, 743, 848
MvnI CGCG 5 cut(s) 640, 666, 712, 734, 888
MwoI GCNNNNNNNGC 9 cut(s) 11, 370, 668, 697, 722, 731, 857, 958, 1011
NciI CCSGG 3 cut(s) 781, 881, 895
NdeII GATC 1 cut(s) 103
NlaIII CATG 4 cut(s) 68, 118, 772, 837
NlaIV GGNNCC 3 cut(s) 785, 903, 962
NmeAIII GCCGAG 2 cut(s) 676, 886
NmuCI GTSAC 2 cut(s) 416, 983
NsbI TGCGCA 2 cut(s) 717, 801
NspI RCATGY 2 cut(s) 118, 837
OliI CACNNNNGTG 1 cut(s) 561
PfeI GAWTC 1 cut(s) 208
PkrI GCNGC 4 cut(s) 549, 580, 670, 733
PleI GAGTC 3 cut(s) 595, 824, 871
PpsI GAGTC 3 cut(s) 595, 824, 871
PpuMI RGGWCCY 1 cut(s) 902
Psp5II RGGWCCY 1 cut(s) 902
Psp6I CCWGG 3 cut(s) 510, 741, 846
PspFI CCCAGC 1 cut(s) 672
PspGI CCWGG 3 cut(s) 510, 741, 846
PspN4I GGNNCC 3 cut(s) 785, 903, 962
PspPI GGNCC 3 cut(s) 625, 745, 902
PspPPI RGGWCCY 1 cut(s) 902
PvuII CAGCTG 1 cut(s) 581
RsaI GTAC 5 cut(s) 20, 98, 148, 872, 976
RsaNI GTAC 5 cut(s) 19, 97, 147, 871, 975
RseI CAYNNNNRTG 2 cut(s) 309, 561
SalI GTCGAC 1 cut(s) 818
SatI GCNGC 4 cut(s) 548, 579, 669, 732
Sau3AI GATC 1 cut(s) 103
Sau96I GGNCC 3 cut(s) 625, 745, 902
ScaI AGTACT 1 cut(s) 148
SchI GAGTC 3 cut(s) 596, 825, 872
ScrFI CCNGG 6 cut(s) 512, 743, 781, 848, 881, 895
SfaNI GCATC 3 cut(s) 388, 607, 847
SfcI CTRYAG 1 cut(s) 524
SinI GGWCC 3 cut(s) 625, 745, 902
SmiMI CAYNNNNRTG 2 cut(s) 309, 561
SmlI CTYRAG 1 cut(s) 1021
SmoI CTYRAG 1 cut(s) 1021
Sse9I AATT 4 cut(s) 368, 488, 602, 1027
SsiI CCGC 7 cut(s) 62, 515, 547, 669, 732, 794, 851
SspI AATATT 1 cut(s) 31
SspMI CTAG 2 cut(s) 906, 1054
StyD4I CCNGG 6 cut(s) 510, 741, 779, 846, 879, 893
StyI CCWWGG 2 cut(s) 705, 762
TaaI ACNGT 2 cut(s) 228, 563
TaqI TCGA 3 cut(s) 249, 819, 1031
TasI AATT 4 cut(s) 368, 488, 602, 1027
TatI WGTACW 2 cut(s) 146, 974
TauI GCSGC 3 cut(s) 550, 671, 734
TfiI GAWTC 1 cut(s) 208
TscAI CASTG 1 cut(s) 193
TseFI GTSAC 2 cut(s) 416, 983
TseI GCWGC 1 cut(s) 578
Tsp45I GTSAC 2 cut(s) 416, 983
TspDTI ATGAA 2 cut(s) 63, 417
TspRI CASTG 1 cut(s) 193
VpaK11BI GGWCC 3 cut(s) 625, 745, 902
XapI RAATTY 1 cut(s) 1027
XceI RCATGY 2 cut(s) 118, 837
XmiI GTMKAC 1 cut(s) 819
XspI CTAG 2 cut(s) 906, 1054
ZrmI AGTACT 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.