MD08G1224900.v1.1

Basic blue

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0003671
Physical Location & Seq
Forward (+)
29007964 .. 29008648
685 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1224900.v1.1.491

Sequence Viewer

Length: 528 bp
ATGGCCACTCAGGTTAGAGGCAGTGCAGTTGTTACCGCAGTAATAGTGATAGAGTTGTGCATGGCTCTGCATGGTGGGATTGGTCATGCCGAAACCTTCACCGTCGGAGAAAATGATGGCTGGACCTTCAACGTCGAGAACTGGACTAAGGGCAAGACCTTTGCTGCCGATGATACATTGGGTAAATTTTACTTTAACTTGTCGCCAACTTATATATTTGTACATTTTTTGTTTTCGACGTATGAGAAAAGGATACTGAGGAAAGGAGAAATTAAAATGAGGAGTGGAGATAGCGGAAGAAATCTAGAGGAATTGATACGAACACAATTTCAAAATGTCAAATACAATAAGGACCTGCACAATGTGGTAGTGGTAGATGAAAATGGTTACAAAACATGCACAGCCATCGGCAAAGAATTATCGTCGGGAAACGATGAAATCAAACTTGCGCGAGGACAAAACTACTTCATCTGCGGTATTCCTGGCCACTGCGATCGTGGCCAAAAGATAGCAGTCACTGCTAATTAA

Protein Analysis

176

Amino Acids

19.49

Weight (kDa)

7.69

Isoelectric Point (pI)

24.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 114 - 168 3.9e-13 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 363
AccII CGCG 1 cut(s) 451
AciI CCGC 3 cut(s) 36, 294, 474
AcoI YGGCCR 3 cut(s) 3, 484, 499
AcsI RAATTY 1 cut(s) 185
AdeI CACNNNGTG 1 cut(s) 364
AfaI GTAC 1 cut(s) 222
AgsI TTSAA 2 cut(s) 130, 332
AjnI CCWGG 1 cut(s) 481
AlwNI CAGNNNCTG 1 cut(s) 518
AoxI GGCC 3 cut(s) 3, 484, 499
ApeKI GCWGC 1 cut(s) 164
ApoI RAATTY 1 cut(s) 185
AspLEI GCGC 1 cut(s) 451
AspS9I GGNCC 2 cut(s) 123, 352
AsuHPI GGTGA 1 cut(s) 91
AvaII GGWCC 2 cut(s) 123, 352
BalI TGGCCA 3 cut(s) 5, 486, 501
BbvI GCAGC 1 cut(s) 151
BccI CCATC 2 cut(s) 110, 413
BcgI CGANNNNNNTGC 2 cut(s) 388, 422
BciT130I CCWGG 1 cut(s) 483
BciVI GTATCC 1 cut(s) 246
BfaI CTAG 1 cut(s) 305
BfuAI ACCTGC 1 cut(s) 363
BfuI GTATCC 1 cut(s) 246
BisI GCNGC 1 cut(s) 165
BlsI GCNGC 1 cut(s) 166
Bme1390I CCNGG 1 cut(s) 483
Bme18I GGWCC 2 cut(s) 123, 352
BmgT120I GGNCC 2 cut(s) 123, 352
BmrFI CCNGG 1 cut(s) 483
Bse1I ACTGG 1 cut(s) 146
BseBI CCWGG 1 cut(s) 483
BseMII CTCAG 2 cut(s) 23, 248
BseNI ACTGG 1 cut(s) 146
BseRI GAGGAG 1 cut(s) 295
BseXI GCAGC 1 cut(s) 151
BsgI GTGCAG 2 cut(s) 45, 341
Bsh1236I CGCG 1 cut(s) 451
Bsh1285I CGRYCG 1 cut(s) 496
BshFI GGCC 3 cut(s) 5, 486, 501
BsiEI CGRYCG 1 cut(s) 496
BsnI GGCC 3 cut(s) 5, 486, 501
Bsp1407I TGTACA 1 cut(s) 220
Bsp143I GATC 1 cut(s) 493
BspACI CCGC 3 cut(s) 36, 294, 474
BspANI GGCC 3 cut(s) 5, 486, 501
BspCNI CTCAG 2 cut(s) 22, 249
BspFNI CGCG 1 cut(s) 451
BspMI ACCTGC 1 cut(s) 363
BsrGI TGTACA 1 cut(s) 220
BsrI ACTGG 1 cut(s) 146
BssMI GATC 1 cut(s) 493
Bst2UI CCWGG 1 cut(s) 483
Bst4CI ACNGT 1 cut(s) 103
BstAPI GCANNNNNTGC 1 cut(s) 518
BstAUI TGTACA 1 cut(s) 220
BstDEI CTNAG 3 cut(s) 9, 147, 257
BstFNI CGCG 1 cut(s) 451
BstHHI GCGC 1 cut(s) 451
BstKTI GATC 1 cut(s) 496
BstMBI GATC 1 cut(s) 493
BstMCI CGRYCG 1 cut(s) 496
BstMWI GCNNNNNNNGC 2 cut(s) 498, 518
BstNI CCWGG 1 cut(s) 483
BstNSI RCATGY 1 cut(s) 399
BstSCI CCNGG 1 cut(s) 481
BstUI CGCG 1 cut(s) 451
BstV1I GCAGC 1 cut(s) 151
BsuI GTATCC 1 cut(s) 246
BsuRI GGCC 3 cut(s) 5, 486, 501
BtsI GCAGTG 3 cut(s) 28, 487, 516
BtsIMutI CAGTG 3 cut(s) 28, 487, 516
BveI ACCTGC 1 cut(s) 363
CaiI CAGNNNCTG 1 cut(s) 518
CfoI GCGC 1 cut(s) 451
Cfr13I GGNCC 2 cut(s) 123, 352
Csp6I GTAC 1 cut(s) 221
CviAII CATG 4 cut(s) 61, 71, 86, 396
CviJI RGCY 6 cut(s) 5, 65, 120, 404, 486, 501
CviKI_1 RGCY 6 cut(s) 5, 65, 120, 404, 486, 501
CviQI GTAC 1 cut(s) 221
DdeI CTNAG 3 cut(s) 9, 147, 257
DpnI GATC 1 cut(s) 495
DpnII GATC 1 cut(s) 493
DraIII CACNNNGTG 1 cut(s) 364
EaeI YGGCCR 3 cut(s) 3, 484, 499
Eco47I GGWCC 2 cut(s) 123, 352
EcoO109I RGGNCCY 1 cut(s) 352
EcoRII CCWGG 1 cut(s) 481
FaeI CATG 4 cut(s) 64, 74, 89, 399
FaiI YATR 7 cut(s) 62, 72, 87, 213, 215, 243, 397
FatI CATG 4 cut(s) 60, 70, 85, 395
Fnu4HI GCNGC 1 cut(s) 165
Fsp4HI GCNGC 1 cut(s) 165
FspBI CTAG 1 cut(s) 305
GlaI GCGC 1 cut(s) 450
GluI GCNGC 1 cut(s) 165
HaeIII GGCC 3 cut(s) 5, 486, 501
HhaI GCGC 1 cut(s) 451
Hin1II CATG 4 cut(s) 64, 74, 89, 399
Hin6I GCGC 1 cut(s) 449
HinP1I GCGC 1 cut(s) 449
HphI GGTGA 1 cut(s) 91
Hpy188I TCNGA 1 cut(s) 107
Hpy188III TCNNGA 3 cut(s) 136, 305, 426
Hpy99I CGWCG 4 cut(s) 107, 137, 241, 427
HpyAV CCTTC 2 cut(s) 106, 136
HpyCH4III ACNGT 1 cut(s) 103
HpyCH4IV ACGT 2 cut(s) 132, 239
HpyCH4V TGCA 5 cut(s) 26, 60, 70, 358, 399
HpyF10VI GCNNNNNNNGC 2 cut(s) 498, 518
HpyF3I CTNAG 3 cut(s) 9, 147, 257
HpySE526I ACGT 2 cut(s) 132, 239
Hsp92II CATG 4 cut(s) 64, 74, 89, 399
HspAI GCGC 1 cut(s) 449
Kzo9I GATC 1 cut(s) 493
LpnPI CCDG 5 cut(s) 106, 127, 368, 468, 495
Lsp1109I GCAGC 1 cut(s) 151
MaeI CTAG 1 cut(s) 305
MaeII ACGT 2 cut(s) 132, 239
MaeIII GTNAC 3 cut(s) 31, 386, 514
MalI GATC 1 cut(s) 495
MboI GATC 1 cut(s) 493
MboII GAAGA 1 cut(s) 309
MlsI TGGCCA 3 cut(s) 5, 486, 501
MluCI AATT 6 cut(s) 185, 270, 311, 326, 416, 523
MluNI TGGCCA 3 cut(s) 5, 486, 501
MmeI TCCRAC 1 cut(s) 85
MnlI CCTC 5 cut(s) 11, 252, 273, 301, 446
Mox20I TGGCCA 3 cut(s) 5, 486, 501
MscI TGGCCA 3 cut(s) 5, 486, 501
MseI TTAA 3 cut(s) 195, 273, 526
Msp20I TGGCCA 3 cut(s) 5, 486, 501
MspR9I CCNGG 1 cut(s) 483
MvaI CCWGG 1 cut(s) 483
MvnI CGCG 1 cut(s) 451
MwoI GCNNNNNNNGC 2 cut(s) 498, 518
NdeII GATC 1 cut(s) 493
NlaIII CATG 4 cut(s) 64, 74, 89, 399
NmuCI GTSAC 1 cut(s) 514
NspI RCATGY 1 cut(s) 399
PkrI GCNGC 1 cut(s) 166
Ple19I CGATCG 1 cut(s) 496
PpuMI RGGWCCY 1 cut(s) 352
Psp5II RGGWCCY 1 cut(s) 352
Psp6I CCWGG 1 cut(s) 481
PspGI CCWGG 1 cut(s) 481
PspPI GGNCC 2 cut(s) 123, 352
PspPPI RGGWCCY 1 cut(s) 352
PstNI CAGNNNCTG 1 cut(s) 518
PvuI CGATCG 1 cut(s) 496
RsaI GTAC 1 cut(s) 222
RsaNI GTAC 1 cut(s) 221
SaqAI TTAA 3 cut(s) 195, 273, 526
SatI GCNGC 1 cut(s) 165
Sau3AI GATC 1 cut(s) 493
Sau96I GGNCC 2 cut(s) 123, 352
ScrFI CCNGG 1 cut(s) 483
SetI ASST 7 cut(s) 15, 98, 128, 135, 161, 242, 357
SinI GGWCC 2 cut(s) 123, 352
Sse9I AATT 6 cut(s) 185, 270, 311, 326, 416, 523
SsiI CCGC 3 cut(s) 36, 294, 474
SspMI CTAG 1 cut(s) 305
StyD4I CCNGG 1 cut(s) 481
TaaI ACNGT 1 cut(s) 103
TaiI ACGT 2 cut(s) 135, 242
TaqI TCGA 2 cut(s) 135, 236
TasI AATT 6 cut(s) 185, 270, 311, 326, 416, 523
TatI WGTACW 1 cut(s) 220
Tru1I TTAA 3 cut(s) 195, 273, 526
Tru9I TTAA 3 cut(s) 195, 273, 526
TscAI CASTG 3 cut(s) 28, 494, 523
TseFI GTSAC 1 cut(s) 514
TseI GCWGC 1 cut(s) 164
Tsp45I GTSAC 1 cut(s) 514
TspDTI ATGAA 3 cut(s) 393, 450, 457
TspRI CASTG 3 cut(s) 28, 494, 523
VpaK11BI GGWCC 2 cut(s) 123, 352
XapI RAATTY 1 cut(s) 185
XbaI TCTAGA 1 cut(s) 304
XceI RCATGY 1 cut(s) 399
XcmI CCANNNNNNNNNTGG 1 cut(s) 494
XspI CTAG 1 cut(s) 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.