pycom05g00630

Basic blue protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003671
Physical Location & Seq
Reverse (-)
767379 .. 768008
630 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g00630.1

Sequence Viewer

Length: 399 bp
ATGCCACAGCGGGGAAGAGGCAGTGAAGGTCGAACCAATGCAACCATGATTATGCAAGTGGCAGTGGTGTTGTGCCTAATGCTTCACCTGGAATATACACATGCTGCTCCTCAGGCTGTTGGAGACTCTAGTGGGTGGACCTTCAACTCCGATAGCTGGCCCAACGGAAAGCACTTTAGAGCCGGCGATGTGCTATTATTCAACTATGATCCAAATCTTCACAATGTGGTAGCTGTGGACAGAGGTGGTTACAGTAGCTGCACAACTCCGAGTGGTGCAAAAGTCTTTAGATCCAGCAAGGACAGAGTAAGGCTAGGGAGGGGACAAAACTACTTCATATGCAGTTTTCCAGGGCACTGCGAGTCTGGGATGAAAGTTGCCATCAATTGTGTCTGGTAA

Protein Analysis

133

Amino Acids

14.5

Weight (kDa)

8.95

Isoelectric Point (pI)

31.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 46 - 124 6.9e-26 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 10
AclWI GGATC 2 cut(s) 203, 285
AdeI CACNNNGTG 1 cut(s) 226
AfiI CCNNNNNNNGG 2 cut(s) 11, 156
AgsI TTSAA 2 cut(s) 145, 202
AjnI CCWGG 2 cut(s) 87, 349
AluBI AGCT 3 cut(s) 156, 233, 258
AluI AGCT 3 cut(s) 156, 233, 258
Alw26I GTCTC 1 cut(s) 117
AlwI GGATC 2 cut(s) 203, 285
AlwNI CAGNNNCTG 1 cut(s) 258
AoxI GGCC 1 cut(s) 158
ApeKI GCWGC 2 cut(s) 104, 258
AspS9I GGNCC 2 cut(s) 138, 159
AsuHPI GGTGA 1 cut(s) 77
AvaII GGWCC 1 cut(s) 138
AxyI CCTNAGG 1 cut(s) 111
BaeGI GKGCMC 1 cut(s) 357
BbvI GCAGC 2 cut(s) 91, 245
BccI CCATC 1 cut(s) 389
BciT130I CCWGG 2 cut(s) 89, 351
BcoDI GTCTC 1 cut(s) 117
BfaI CTAG 2 cut(s) 129, 314
BisI GCNGC 2 cut(s) 105, 259
BlsI GCNGC 2 cut(s) 106, 260
Bme1390I CCNGG 2 cut(s) 89, 351
Bme18I GGWCC 1 cut(s) 138
BmgT120I GGNCC 2 cut(s) 138, 159
BmrFI CCNGG 2 cut(s) 89, 351
BsaBI GATNNNNATC 1 cut(s) 213
BsaJI CCNNGG 1 cut(s) 350
Bsc4I CCNNNNNNNGG 2 cut(s) 11, 156
Bse118I RCCGGY 1 cut(s) 182
Bse21I CCTNAGG 1 cut(s) 111
Bse8I GATNNNNATC 1 cut(s) 213
BseBI CCWGG 2 cut(s) 89, 351
BseDI CCNNGG 1 cut(s) 350
BseGI GGATG 1 cut(s) 375
BseJI GATNNNNATC 1 cut(s) 213
BseLI CCNNNNNNNGG 2 cut(s) 11, 156
BseMII CTCAG 1 cut(s) 125
BseRI GAGGAG 1 cut(s) 99
BseSI GKGCMC 1 cut(s) 357
BseXI GCAGC 2 cut(s) 91, 245
BsgI GTGCAG 1 cut(s) 244
BshFI GGCC 1 cut(s) 160
BsiSI CCGG 1 cut(s) 183
BslFI GGGAC 1 cut(s) 336
BslI CCNNNNNNNGG 2 cut(s) 11, 156
BsmAI GTCTC 1 cut(s) 117
BsmFI GGGAC 1 cut(s) 336
BsnI GGCC 1 cut(s) 160
Bsp1286I GDGCHC 1 cut(s) 357
Bsp143I GATC 2 cut(s) 208, 290
BspACI CCGC 1 cut(s) 10
BspANI GGCC 1 cut(s) 160
BspCNI CTCAG 1 cut(s) 124
BspPI GGATC 2 cut(s) 203, 285
BsrFI RCCGGY 1 cut(s) 182
BssAI RCCGGY 1 cut(s) 182
BssECI CCNNGG 1 cut(s) 350
BssMI GATC 2 cut(s) 208, 290
Bst2UI CCWGG 2 cut(s) 89, 351
Bst4CI ACNGT 1 cut(s) 254
Bst6I CTCTTC 1 cut(s) 10
BstC8I GCNNGC 2 cut(s) 158, 184
BstDEI CTNAG 1 cut(s) 111
BstF5I GGATG 1 cut(s) 375
BstKTI GATC 2 cut(s) 211, 293
BstMAI GTCTC 1 cut(s) 117
BstMBI GATC 2 cut(s) 208, 290
BstMWI GCNNNNNNNGC 1 cut(s) 113
BstNI CCWGG 2 cut(s) 89, 351
BstNSI RCATGY 1 cut(s) 104
BstSCI CCNGG 2 cut(s) 87, 349
BstSLI GKGCMC 1 cut(s) 357
BstV1I GCAGC 2 cut(s) 91, 245
BstX2I RGATCY 1 cut(s) 290
BstYI RGATCY 1 cut(s) 290
Bsu36I CCTNAGG 1 cut(s) 111
BsuRI GGCC 1 cut(s) 160
BtgZI GCGATG 1 cut(s) 201
BtsCI GGATG 1 cut(s) 375
BtsI GCAGTG 3 cut(s) 28, 69, 355
BtsIMutI CAGTG 3 cut(s) 28, 69, 355
Cac8I GCNNGC 2 cut(s) 158, 184
CaiI CAGNNNCTG 1 cut(s) 258
Cfr10I RCCGGY 1 cut(s) 182
Cfr13I GGNCC 2 cut(s) 138, 159
CviAII CATG 2 cut(s) 46, 101
CviJI RGCY 7 cut(s) 116, 156, 160, 182, 233, 258, 313
CviKI_1 RGCY 7 cut(s) 116, 156, 160, 182, 233, 258, 313
DdeI CTNAG 1 cut(s) 111
DpnI GATC 2 cut(s) 210, 292
DpnII GATC 2 cut(s) 208, 290
DraIII CACNNNGTG 1 cut(s) 226
Eam1104I CTCTTC 1 cut(s) 10
EarI CTCTTC 1 cut(s) 10
Eco47I GGWCC 1 cut(s) 138
Eco81I CCTNAGG 1 cut(s) 111
EcoRII CCWGG 2 cut(s) 87, 349
FaeI CATG 2 cut(s) 49, 104
FaiI YATR 7 cut(s) 47, 53, 96, 102, 207, 338, 340
FaqI GGGAC 1 cut(s) 336
FatI CATG 2 cut(s) 45, 100
FauI CCCGC 1 cut(s) 3
FauNDI CATATG 1 cut(s) 338
Fnu4HI GCNGC 2 cut(s) 105, 259
FokI GGATG 1 cut(s) 382
Fsp4HI GCNGC 2 cut(s) 105, 259
FspBI CTAG 2 cut(s) 129, 314
GluI GCNGC 2 cut(s) 105, 259
HaeIII GGCC 1 cut(s) 160
HapII CCGG 1 cut(s) 183
Hin1II CATG 2 cut(s) 49, 104
HinfI GANTC 2 cut(s) 125, 362
HpaII CCGG 1 cut(s) 183
HphI GGTGA 1 cut(s) 77
Hpy166II GTNNAC 2 cut(s) 138, 238
Hpy188I TCNGA 2 cut(s) 151, 270
Hpy8I GTNNAC 2 cut(s) 138, 238
HpyAV CCTTC 2 cut(s) 20, 151
HpyCH4III ACNGT 1 cut(s) 254
HpyCH4V TGCA 5 cut(s) 41, 55, 261, 278, 342
HpyF10VI GCNNNNNNNGC 1 cut(s) 113
HpyF3I CTNAG 1 cut(s) 111
Hsp92II CATG 2 cut(s) 49, 104
KroI GCCGGC 1 cut(s) 182
KroNI GCCGGC 1 cut(s) 184
Kzo9I GATC 2 cut(s) 208, 290
LmnI GCTCC 1 cut(s) 112
Lsp1109I GCAGC 2 cut(s) 91, 245
MaeI CTAG 2 cut(s) 129, 314
MaeIII GTNAC 1 cut(s) 248
MalI GATC 2 cut(s) 210, 292
MboI GATC 2 cut(s) 208, 290
MboII GAAGA 2 cut(s) 27, 209
MfeI CAATTG 1 cut(s) 385
MflI RGATCY 1 cut(s) 290
MhlI GDGCHC 1 cut(s) 357
MluCI AATT 1 cut(s) 385
MlyI GAGTC 2 cut(s) 119, 371
MmeI TCCRAC 1 cut(s) 100
MnlI CCTC 4 cut(s) 11, 120, 236, 312
MroNI GCCGGC 1 cut(s) 182
MslI CAYNNNNRTG 1 cut(s) 50
MspA1I CMGCKG 1 cut(s) 10
MspI CCGG 1 cut(s) 183
MspR9I CCNGG 2 cut(s) 89, 351
MunI CAATTG 1 cut(s) 385
MvaI CCWGG 2 cut(s) 89, 351
MwoI GCNNNNNNNGC 1 cut(s) 113
NaeI GCCGGC 1 cut(s) 184
NdeI CATATG 1 cut(s) 338
NdeII GATC 2 cut(s) 208, 290
NgoMIV GCCGGC 1 cut(s) 182
NlaIII CATG 2 cut(s) 49, 104
NspI RCATGY 1 cut(s) 104
PdiI GCCGGC 1 cut(s) 184
PkrI GCNGC 2 cut(s) 106, 260
PleI GAGTC 2 cut(s) 119, 370
PpsI GAGTC 2 cut(s) 119, 370
Psp6I CCWGG 2 cut(s) 87, 349
PspGI CCWGG 2 cut(s) 87, 349
PspPI GGNCC 2 cut(s) 138, 159
PstNI CAGNNNCTG 1 cut(s) 258
PsuI RGATCY 1 cut(s) 290
RseI CAYNNNNRTG 1 cut(s) 50
SatI GCNGC 2 cut(s) 105, 259
Sau3AI GATC 2 cut(s) 208, 290
Sau96I GGNCC 2 cut(s) 138, 159
SchI GAGTC 2 cut(s) 119, 371
ScrFI CCNGG 2 cut(s) 89, 351
SduI GDGCHC 1 cut(s) 357
SetI ASST 7 cut(s) 31, 90, 143, 158, 235, 247, 260
SinI GGWCC 1 cut(s) 138
SmiMI CAYNNNNRTG 1 cut(s) 50
Sse9I AATT 1 cut(s) 385
SsiI CCGC 1 cut(s) 10
SspMI CTAG 2 cut(s) 129, 314
StyD4I CCNGG 2 cut(s) 87, 349
TaaI ACNGT 1 cut(s) 254
TaqI TCGA 1 cut(s) 31
TasI AATT 1 cut(s) 385
TscAI CASTG 3 cut(s) 28, 69, 362
TseI GCWGC 2 cut(s) 104, 258
TspDTI ATGAA 2 cut(s) 325, 386
TspGWI ACGGA 1 cut(s) 180
TspRI CASTG 3 cut(s) 28, 69, 362
VpaK11BI GGWCC 1 cut(s) 138
XceI RCATGY 1 cut(s) 104
XspI CTAG 2 cut(s) 129, 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.