MD15G1418000.v1.1

Basic blue

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003671
Physical Location & Seq
Forward (+)
51985800 .. 51987212
1413 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1418000.v1.1.491

Sequence Viewer

Length: 369 bp
ATGGCCACCCAGCTTAGAGGCAGTGCAATTGTTACCGCAGTGATAGCGATAGGGTTGGTGCTTCACGGTGAGATTGCTCATGCCAAAACCTTCACCGTCGGAGAGGGTGATGGCTGGAACTTCAAGGTTCATGACTGGCCTAATGGCAAGAACTTTACAGCCAATGATACGTTGGTGTTCAAATACAATAATGCATTCCACAATGTGGCAGTCGTAGATGAAAATGGTTACAGGACATGCACAGTCGGCGGCAAGCTCTTGTCGACGGGAAACGATGTGATCAAAATTCAGCAAGGACAAAACTACTTCATCTGCGGTTTTCCTGGCCACTGTGCTGCTGGGATGAAGATAGCAGTCACTGCGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

123

Amino Acids

13.05

Weight (kDa)

8.49

Isoelectric Point (pI)

0.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 41 - 115 1.1e-20 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 205
AccI GTMKAC 1 cut(s) 263
AciI CCGC 3 cut(s) 36, 249, 315
AcoI YGGCCR 2 cut(s) 3, 325
AcsI RAATTY 1 cut(s) 285
AdeI CACNNNGTG 1 cut(s) 205
AfiI CCNNNNNNNGG 1 cut(s) 205
AgsI TTSAA 2 cut(s) 124, 181
AjnI CCWGG 1 cut(s) 322
AluBI AGCT 2 cut(s) 13, 256
AluI AGCT 2 cut(s) 13, 256
AlwNI CAGNNNCTG 1 cut(s) 359
AoxI GGCC 3 cut(s) 3, 137, 325
ApeKI GCWGC 1 cut(s) 335
ApoI RAATTY 1 cut(s) 285
AsuHPI GGTGA 3 cut(s) 80, 85, 119
BaeI ACNNNNGTAYC 2 cut(s) 159, 192
BalI TGGCCA 2 cut(s) 5, 327
BbvI GCAGC 1 cut(s) 322
BccI CCATC 1 cut(s) 104
BciT130I CCWGG 1 cut(s) 324
BclI TGATCA 1 cut(s) 279
BisI GCNGC 2 cut(s) 250, 336
BlsI GCNGC 2 cut(s) 251, 337
Bme1390I CCNGG 1 cut(s) 324
BmrFI CCNGG 1 cut(s) 324
Bsc4I CCNNNNNNNGG 1 cut(s) 205
Bse1I ACTGG 1 cut(s) 140
BseBI CCWGG 1 cut(s) 324
BseGI GGATG 1 cut(s) 348
BseLI CCNNNNNNNGG 1 cut(s) 205
BseNI ACTGG 1 cut(s) 140
BseXI GCAGC 1 cut(s) 322
BseYI CCCAGC 2 cut(s) 9, 338
BshFI GGCC 3 cut(s) 5, 139, 327
BslI CCNNNNNNNGG 1 cut(s) 205
BsmI GAATGC 1 cut(s) 194
BsnI GGCC 3 cut(s) 5, 139, 327
Bsp143I GATC 1 cut(s) 279
BspACI CCGC 3 cut(s) 36, 249, 315
BspANI GGCC 3 cut(s) 5, 139, 327
BspHI TCATGA 1 cut(s) 130
BsrI ACTGG 1 cut(s) 140
BssMI GATC 1 cut(s) 279
Bst2UI CCWGG 1 cut(s) 324
Bst4CI ACNGT 4 cut(s) 68, 97, 244, 332
BstAPI GCANNNNNTGC 1 cut(s) 359
BstC8I GCNNGC 1 cut(s) 254
BstDEI CTNAG 1 cut(s) 14
BstF5I GGATG 1 cut(s) 348
BstKTI GATC 1 cut(s) 282
BstMBI GATC 1 cut(s) 279
BstMWI GCNNNNNNNGC 3 cut(s) 44, 246, 359
BstNI CCWGG 1 cut(s) 324
BstNSI RCATGY 1 cut(s) 240
BstSCI CCNGG 1 cut(s) 322
BstV1I GCAGC 1 cut(s) 322
BsuRI GGCC 3 cut(s) 5, 139, 327
BtsCI GGATG 1 cut(s) 348
BtsI GCAGTG 3 cut(s) 28, 45, 357
BtsIMutI CAGTG 4 cut(s) 28, 45, 328, 357
Cac8I GCNNGC 1 cut(s) 254
CaiI CAGNNNCTG 1 cut(s) 359
CciI TCATGA 1 cut(s) 130
CviAII CATG 3 cut(s) 80, 131, 237
CviJI RGCY 7 cut(s) 5, 13, 114, 139, 161, 256, 327
CviKI_1 RGCY 7 cut(s) 5, 13, 114, 139, 161, 256, 327
DdeI CTNAG 1 cut(s) 14
DpnI GATC 1 cut(s) 281
DpnII GATC 1 cut(s) 279
DraIII CACNNNGTG 1 cut(s) 205
EaeI YGGCCR 2 cut(s) 3, 325
EcoRII CCWGG 1 cut(s) 322
EcoT22I ATGCAT 1 cut(s) 196
FaeI CATG 3 cut(s) 83, 134, 240
FaiI YATR 3 cut(s) 81, 132, 238
FatI CATG 3 cut(s) 79, 130, 236
FbaI TGATCA 1 cut(s) 279
FblI GTMKAC 1 cut(s) 263
Fnu4HI GCNGC 2 cut(s) 250, 336
FokI GGATG 1 cut(s) 355
Fsp4HI GCNGC 2 cut(s) 250, 336
GluI GCNGC 2 cut(s) 250, 336
GsaI CCCAGC 2 cut(s) 13, 342
HaeIII GGCC 3 cut(s) 5, 139, 327
Hin1II CATG 3 cut(s) 83, 134, 240
HincII GTYRAC 1 cut(s) 264
HindII GTYRAC 1 cut(s) 264
HphI GGTGA 3 cut(s) 80, 85, 119
Hpy166II GTNNAC 1 cut(s) 264
Hpy188I TCNGA 1 cut(s) 101
Hpy188III TCNNGA 1 cut(s) 131
Hpy8I GTNNAC 1 cut(s) 264
Hpy99I CGWCG 2 cut(s) 101, 268
HpyAV CCTTC 1 cut(s) 100
HpyCH4III ACNGT 4 cut(s) 68, 97, 244, 332
HpyCH4IV ACGT 1 cut(s) 170
HpyCH4V TGCA 3 cut(s) 26, 194, 240
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 246, 359
HpyF3I CTNAG 1 cut(s) 14
HpySE526I ACGT 1 cut(s) 170
Hsp92II CATG 3 cut(s) 83, 134, 240
Ksp22I TGATCA 1 cut(s) 279
Kzo9I GATC 1 cut(s) 279
LpnPI CCDG 7 cut(s) 23, 100, 121, 217, 309, 324, 336
Lsp1109I GCAGC 1 cut(s) 322
MaeII ACGT 1 cut(s) 170
MaeIII GTNAC 3 cut(s) 31, 227, 355
MalI GATC 1 cut(s) 281
MboI GATC 1 cut(s) 279
MboII GAAGA 1 cut(s) 358
MfeI CAATTG 1 cut(s) 27
MlsI TGGCCA 2 cut(s) 5, 327
MluCI AATT 2 cut(s) 27, 285
MluNI TGGCCA 2 cut(s) 5, 327
MmeI TCCRAC 1 cut(s) 79
MnlI CCTC 2 cut(s) 11, 97
Mox20I TGGCCA 2 cut(s) 5, 327
Mph1103I ATGCAT 1 cut(s) 196
MscI TGGCCA 2 cut(s) 5, 327
Msp20I TGGCCA 2 cut(s) 5, 327
MspR9I CCNGG 1 cut(s) 324
MunI CAATTG 1 cut(s) 27
Mva1269I GAATGC 1 cut(s) 194
MvaI CCWGG 1 cut(s) 324
MwoI GCNNNNNNNGC 3 cut(s) 44, 246, 359
NdeII GATC 1 cut(s) 279
NlaIII CATG 3 cut(s) 83, 134, 240
NmuCI GTSAC 1 cut(s) 355
NsiI ATGCAT 1 cut(s) 196
NspI RCATGY 1 cut(s) 240
PagI TCATGA 1 cut(s) 130
PctI GAATGC 1 cut(s) 194
PflMI CCANNNNNTGG 1 cut(s) 205
PkrI GCNGC 2 cut(s) 251, 337
Psp6I CCWGG 1 cut(s) 322
PspFI CCCAGC 2 cut(s) 9, 338
PspGI CCWGG 1 cut(s) 322
PstNI CAGNNNCTG 1 cut(s) 359
SalI GTCGAC 1 cut(s) 262
SatI GCNGC 2 cut(s) 250, 336
Sau3AI GATC 1 cut(s) 279
ScrFI CCNGG 1 cut(s) 324
SetI ASST 5 cut(s) 15, 92, 129, 173, 258
Sse9I AATT 2 cut(s) 27, 285
SsiI CCGC 3 cut(s) 36, 249, 315
StyD4I CCNGG 1 cut(s) 322
TaaI ACNGT 4 cut(s) 68, 97, 244, 332
TaiI ACGT 1 cut(s) 173
TaqI TCGA 1 cut(s) 263
TasI AATT 2 cut(s) 27, 285
TauI GCSGC 1 cut(s) 252
TscAI CASTG 4 cut(s) 28, 45, 335, 364
TseFI GTSAC 1 cut(s) 355
TseI GCWGC 1 cut(s) 335
Tsp45I GTSAC 1 cut(s) 355
TspDTI ATGAA 4 cut(s) 119, 234, 298, 359
TspRI CASTG 4 cut(s) 28, 45, 335, 364
Van91I CCANNNNNTGG 1 cut(s) 205
XapI RAATTY 1 cut(s) 285
XceI RCATGY 1 cut(s) 240
XcmI CCANNNNNNNNNTGG 2 cut(s) 169, 335
XmiI GTMKAC 1 cut(s) 263
Zsp2I ATGCAT 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.