RchiOBHm_Chr7g0224441

Basic blue protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0003671
Physical Location & Seq
Forward (+)
46580918 .. 46582623
1706 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20097

Sequence Viewer

Length: 375 bp
ATGGCTATGGGAAGAGGCAGTGCAACGGCGGTGCTCGTGGTGTTTGTGTTGGTGGTGCTGAGTTCGGAGTGGGCCGATGCGGCGACCTACACTGTTGGAGACAGAGGTGGTTGGACCTTCAACGTTGCTGCGTGGCCGAGAGGAAAGACTTTCCGAGCCGGTGACATACTTGTTTTCAACTATGCATCTGCCGCTCACAATGTGGTAGCTGTGAACAAAGTGGGGTACCAGAAATGTAGTACAACTCCAGGAAATGCCAAAGTGTATCAAACTGGGAAAGATCGCATCAGACTTGCGAAAGGACAGAACTTCTTCATCTGTAGTTTCCCTGGACACTGCCAATCTGGCATGAAAGTTGCCGTTACTGCAACCTAG

Protein Analysis

124

Amino Acids

13.21

Weight (kDa)

9.82

Isoelectric Point (pI)

10.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 38 - 117 2.5e-26 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 225
AccB1I GGYRCC 1 cut(s) 225
AccBSI CCGCTC 1 cut(s) 194
AciI CCGC 3 cut(s) 29, 80, 192
AclI AACGTT 1 cut(s) 123
AcoI YGGCCR 1 cut(s) 134
AdeI CACNNNGTG 1 cut(s) 202
AfaI GTAC 2 cut(s) 227, 241
AgsI TTSAA 2 cut(s) 121, 178
AjnI CCWGG 2 cut(s) 247, 328
AluBI AGCT 1 cut(s) 209
AluI AGCT 1 cut(s) 209
Alw21I GWGCWC 1 cut(s) 36
Alw26I GTCTC 1 cut(s) 93
AoxI GGCC 2 cut(s) 72, 134
ApeKI GCWGC 1 cut(s) 128
Asp700I GAANNNNTTC 1 cut(s) 311
Asp718I GGTACC 1 cut(s) 225
AspS9I GGNCC 2 cut(s) 72, 114
AsuHPI GGTGA 1 cut(s) 173
AvaII GGWCC 1 cut(s) 114
BanI GGYRCC 1 cut(s) 225
BauI CACGAG 1 cut(s) 35
Bbv12I GWGCWC 1 cut(s) 36
BbvI GCAGC 1 cut(s) 115
BceAI ACGGC 2 cut(s) 42, 344
BciT130I CCWGG 2 cut(s) 249, 330
BcoDI GTCTC 1 cut(s) 93
BfaI CTAG 1 cut(s) 373
BfmI CTRYAG 1 cut(s) 319
BglI GCCNNNNNGGC 2 cut(s) 80, 345
BisI GCNGC 3 cut(s) 81, 129, 192
BlsI GCNGC 3 cut(s) 82, 130, 193
Bme1390I CCNGG 2 cut(s) 249, 330
Bme18I GGWCC 1 cut(s) 114
BmgT120I GGNCC 2 cut(s) 72, 114
BmiI GGNNCC 1 cut(s) 227
BmrFI CCNGG 2 cut(s) 249, 330
BmrI ACTGGG 1 cut(s) 282
BmsI GCATC 3 cut(s) 67, 194, 294
BmuI ACTGGG 1 cut(s) 282
BpmI CTGGAG 1 cut(s) 231
BsaJI CCNNGG 1 cut(s) 328
Bse118I RCCGGY 1 cut(s) 158
Bse1I ACTGG 1 cut(s) 277
BseBI CCWGG 2 cut(s) 249, 330
BseDI CCNNGG 1 cut(s) 328
BseMII CTCAG 1 cut(s) 50
BseNI ACTGG 1 cut(s) 277
BseXI GCAGC 1 cut(s) 115
BshFI GGCC 2 cut(s) 74, 136
BshNI GGYRCC 1 cut(s) 225
BsiHKAI GWGCWC 1 cut(s) 36
BsiSI CCGG 1 cut(s) 159
BsmAI GTCTC 1 cut(s) 93
BsnI GGCC 2 cut(s) 74, 136
Bsp1286I GDGCHC 1 cut(s) 36
Bsp143I GATC 1 cut(s) 280
BspACI CCGC 3 cut(s) 29, 80, 192
BspANI GGCC 2 cut(s) 74, 136
BspCNI CTCAG 1 cut(s) 51
BspLI GGNNCC 1 cut(s) 227
BspT107I GGYRCC 1 cut(s) 225
BsrBI CCGCTC 1 cut(s) 194
BsrFI RCCGGY 1 cut(s) 158
BsrI ACTGG 1 cut(s) 277
BssAI RCCGGY 1 cut(s) 158
BssECI CCNNGG 1 cut(s) 328
BssMI GATC 1 cut(s) 280
BssSI CACGAG 1 cut(s) 35
Bst2BI CACGAG 1 cut(s) 35
Bst2UI CCWGG 2 cut(s) 249, 330
Bst4CI ACNGT 1 cut(s) 94
Bst6I CTCTTC 1 cut(s) 7
BstDEI CTNAG 1 cut(s) 59
BstKTI GATC 1 cut(s) 283
BstMAI GTCTC 1 cut(s) 93
BstMBI GATC 1 cut(s) 280
BstMWI GCNNNNNNNGC 4 cut(s) 80, 191, 345, 365
BstNI CCWGG 2 cut(s) 249, 330
BstSCI CCNGG 2 cut(s) 247, 328
BstSFI CTRYAG 1 cut(s) 319
BstV1I GCAGC 1 cut(s) 115
BsuRI GGCC 2 cut(s) 74, 136
BtsI GCAGTG 2 cut(s) 25, 334
BtsIMutI CAGTG 3 cut(s) 25, 90, 334
Cfr10I RCCGGY 1 cut(s) 158
Cfr13I GGNCC 2 cut(s) 72, 114
Csp6I GTAC 2 cut(s) 226, 240
CviAII CATG 1 cut(s) 349
CviJI RGCY 5 cut(s) 5, 74, 136, 158, 209
CviKI_1 RGCY 5 cut(s) 5, 74, 136, 158, 209
CviQI GTAC 2 cut(s) 226, 240
DdeI CTNAG 1 cut(s) 59
DpnI GATC 1 cut(s) 282
DpnII GATC 1 cut(s) 280
DraIII CACNNNGTG 1 cut(s) 202
EaeI YGGCCR 1 cut(s) 134
Eam1104I CTCTTC 1 cut(s) 7
EarI CTCTTC 1 cut(s) 7
Eco47I GGWCC 1 cut(s) 114
EcoRII CCWGG 2 cut(s) 247, 328
EcoT22I ATGCAT 1 cut(s) 187
FaeI CATG 1 cut(s) 352
FaiI YATR 4 cut(s) 8, 167, 183, 350
FatI CATG 1 cut(s) 348
Fnu4HI GCNGC 3 cut(s) 81, 129, 192
Fsp4HI GCNGC 3 cut(s) 81, 129, 192
FspBI CTAG 1 cut(s) 373
GluI GCNGC 3 cut(s) 81, 129, 192
GsuI CTGGAG 1 cut(s) 231
HaeIII GGCC 2 cut(s) 74, 136
HapII CCGG 1 cut(s) 159
Hin1II CATG 1 cut(s) 352
HpaII CCGG 1 cut(s) 159
HphI GGTGA 1 cut(s) 173
Hpy166II GTNNAC 1 cut(s) 214
Hpy188I TCNGA 3 cut(s) 67, 155, 290
Hpy8I GTNNAC 1 cut(s) 214
HpyAV CCTTC 1 cut(s) 127
HpyCH4III ACNGT 1 cut(s) 94
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 3 cut(s) 23, 185, 368
HpyF10VI GCNNNNNNNGC 4 cut(s) 80, 191, 345, 365
HpyF3I CTNAG 1 cut(s) 59
HpySE526I ACGT 1 cut(s) 123
Hsp92II CATG 1 cut(s) 352
KpnI GGTACC 1 cut(s) 229
Kzo9I GATC 1 cut(s) 280
LpnPI CCDG 8 cut(s) 172, 234, 242, 258, 261, 315, 330, 342
Lsp1109I GCAGC 1 cut(s) 115
LweI GCATC 3 cut(s) 67, 194, 294
MaeI CTAG 1 cut(s) 373
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 2 cut(s) 161, 361
MalI GATC 1 cut(s) 282
MbiI CCGCTC 1 cut(s) 194
MboI GATC 1 cut(s) 280
MboII GAAGA 2 cut(s) 24, 304
MhlI GDGCHC 1 cut(s) 36
MmeI TCCRAC 2 cut(s) 76, 92
MnlI CCTC 3 cut(s) 8, 98, 134
Mph1103I ATGCAT 1 cut(s) 187
MroXI GAANNNNTTC 1 cut(s) 311
MspI CCGG 1 cut(s) 159
MspR9I CCNGG 2 cut(s) 249, 330
MvaI CCWGG 2 cut(s) 249, 330
MwoI GCNNNNNNNGC 4 cut(s) 80, 191, 345, 365
NdeII GATC 1 cut(s) 280
NlaIII CATG 1 cut(s) 352
NlaIV GGNNCC 1 cut(s) 227
NmeAIII GCCGAG 1 cut(s) 162
NmuCI GTSAC 1 cut(s) 161
NsiI ATGCAT 1 cut(s) 187
PdmI GAANNNNTTC 1 cut(s) 311
PfoI TCCNGGA 1 cut(s) 247
PkrI GCNGC 3 cut(s) 82, 130, 193
Psp1406I AACGTT 1 cut(s) 123
Psp6I CCWGG 2 cut(s) 247, 328
PspGI CCWGG 2 cut(s) 247, 328
PspN4I GGNNCC 1 cut(s) 227
PspPI GGNCC 2 cut(s) 72, 114
RsaI GTAC 2 cut(s) 227, 241
RsaNI GTAC 2 cut(s) 226, 240
SatI GCNGC 3 cut(s) 81, 129, 192
Sau3AI GATC 1 cut(s) 280
Sau96I GGNCC 2 cut(s) 72, 114
ScrFI CCNGG 2 cut(s) 249, 330
SduI GDGCHC 1 cut(s) 36
SetI ASST 6 cut(s) 89, 109, 119, 126, 211, 374
SfaNI GCATC 3 cut(s) 67, 194, 294
SfcI CTRYAG 1 cut(s) 319
SinI GGWCC 1 cut(s) 114
SsiI CCGC 3 cut(s) 29, 80, 192
SspMI CTAG 1 cut(s) 373
StyD4I CCNGG 2 cut(s) 247, 328
TaaI ACNGT 1 cut(s) 94
TaiI ACGT 1 cut(s) 126
TatI WGTACW 1 cut(s) 239
TauI GCSGC 2 cut(s) 83, 194
TscAI CASTG 3 cut(s) 25, 97, 341
TseFI GTSAC 1 cut(s) 161
TseI GCWGC 1 cut(s) 128
Tsp45I GTSAC 1 cut(s) 161
TspDTI ATGAA 2 cut(s) 304, 365
TspRI CASTG 3 cut(s) 25, 97, 341
VpaK11BI GGWCC 1 cut(s) 114
XmnI GAANNNNTTC 1 cut(s) 311
XspI CTAG 1 cut(s) 373
Zsp2I ATGCAT 1 cut(s) 187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.