FvH4_5g36240

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0002598
Physical Location & Seq
Forward (+)
26565555 .. 26567963
2409 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g36240.t2

Sequence Viewer

Length: 1023 bp
ATGGCATTAGAAGCTGTGGTTTATCCAAAAGACTACAACCCATTTAGCTATGTGTCCAAAGACTTCTACTCCCTTGCGAACTGGGGCTATGATTTTAGCTTACAAGAGGATCCTCAAGACAAAGCCCTCGTTGGACTTCTTGATCATCAGAACAACAACATACAGCACCACCAACAAGGCGGTCTCGATGAAAATTGGGACTATAACTGCTCATCATCTGCTCCTTCATTTCTGCATAATGTGAAGGAATGGGATCCTCCTCATTCCTCACCCGAGGCCCTGGAAACACCGCCACCGCCACCGCCACAGGCAACAACAGGTAGACGCAAACGCCGTCGTACCAGAAGCAGCAAGAACAAAGAAGAAATGGAGAACCAACGCATGACCCACATTGTAGTAGAGCGCAACCGCCGCAAGCAAATGAACGAGTACCTCGCCGTCCTCCGCTCTTTGATGCCAACTTCTTATGTTCAAAGGGGAGATCAAGCATCAATTATTGGGGGAGCTATCAACTTTGTGAAGGAGCTAGAGCAACTGTTGCAGTCCATGGACGGCAGCCATAAGAGGACCAGCAAGCCGGAACAAGTACCTTTGGCTGAGTTCTTCACCTACCCACAGTTCTCCACGTATGCAACAACACAGTGTATCAGAAACTCTTCTTCATCTTCTTCATCATCATCTGGGTGTATCCAAGTAAACGACGAGCCGTCCATGGCTCCTCGGAGCAACAATAACAACGACAACCAGTGGCCAGCTGCAGACATAGAAGTGACCATGGTGGACAGCCATGCCAATCTTAAGATATTGTCCAAGAAGAGGCCGAGGCAGTTGTTGAAGATGGTGGCCGGGTTTCAGAGTCTTAGGCTCACTGTCCTTCACCTCAATGTCACCACTGTTGATGAAATGGTCCTCTATTCTGTTAGTGTTAAGATGGAAGAAGGATGCATGCTTAACACGGTGGATGAAATCGCAGCTGCTGTGAATGAAATGCTGCGCAGAATTCAAGAAGCTAGTATTAGCTGA

Protein Analysis

341

Amino Acids

38.51

Weight (kDa)

6.16

Isoelectric Point (pI)

64.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 126 - 177 6.9e-12 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 995
AccBSI CCGCTC 1 cut(s) 447
AccI GTMKAC 1 cut(s) 322
AciI CCGC 7 cut(s) 180, 290, 296, 302, 409, 412, 445
AclWI GGATC 4 cut(s) 104, 117, 248, 261
AcoI YGGCCR 2 cut(s) 749, 843
AcsI RAATTY 1 cut(s) 999
AfaI GTAC 3 cut(s) 340, 431, 588
AfiI CCNNNNNNNGG 1 cut(s) 816
AflII CTTAAG 1 cut(s) 797
AgsI TTSAA 3 cut(s) 473, 835, 1004
AhdI GACNNNNNGTC 1 cut(s) 706
AjnI CCWGG 1 cut(s) 279
AluBI AGCT 9 cut(s) 14, 48, 99, 506, 526, 755, 974, 1010, 1020
AluI AGCT 9 cut(s) 14, 48, 99, 506, 526, 755, 974, 1010, 1020
Alw26I GTCTC 1 cut(s) 188
AlwI GGATC 4 cut(s) 104, 117, 248, 261
AlwNI CAGNNNCTG 1 cut(s) 977
Ama87I CYCGRG 1 cut(s) 272
AoxI GGCC 4 cut(s) 276, 749, 818, 843
ApeKI GCWGC 6 cut(s) 348, 555, 755, 971, 974, 991
ApoI RAATTY 1 cut(s) 999
Asp700I GAANNNNTTC 1 cut(s) 655
AspLEI GCGC 2 cut(s) 405, 996
AspS9I GGNCC 3 cut(s) 277, 567, 907
AsuC2I CCSGG 1 cut(s) 847
AsuHPI GGTGA 4 cut(s) 261, 598, 869, 880
AvaI CYCGRG 1 cut(s) 272
AvaII GGWCC 2 cut(s) 567, 907
BalI TGGCCA 1 cut(s) 751
BamHI GGATCC 2 cut(s) 109, 253
BbvI GCAGC 6 cut(s) 360, 567, 742, 961, 978, 983
BccI CCATC 2 cut(s) 832, 925
BceAI ACGGC 4 cut(s) 318, 422, 568, 691
BciT130I CCWGG 1 cut(s) 281
BciVI GTATCC 1 cut(s) 698
BclI TGATCA 1 cut(s) 142
BcnI CCSGG 1 cut(s) 847
BcoDI GTCTC 1 cut(s) 188
BfaI CTAG 2 cut(s) 527, 1011
BfmI CTRYAG 1 cut(s) 756
BfrI CTTAAG 1 cut(s) 797
BfuI GTATCC 1 cut(s) 698
BisI GCNGC 7 cut(s) 349, 412, 556, 756, 972, 975, 992
BlsI GCNGC 7 cut(s) 350, 413, 557, 757, 973, 976, 993
Bme1390I CCNGG 2 cut(s) 281, 847
Bme18I GGWCC 2 cut(s) 567, 907
BmeRI GACNNNNNGTC 1 cut(s) 706
BmeT110I CYCGRG 1 cut(s) 272
BmgT120I GGNCC 3 cut(s) 277, 567, 907
BmiI GGNNCC 3 cut(s) 111, 255, 717
BmrFI CCNGG 2 cut(s) 281, 847
BmrI ACTGGG 1 cut(s) 91
BmsI GCATC 3 cut(s) 444, 497, 932
BmuI ACTGGG 1 cut(s) 91
BpuEI CTTGAG 1 cut(s) 99
BpuMI CCSGG 1 cut(s) 847
BsaAI YACGTR 1 cut(s) 627
BsaI GGTCTC 1 cut(s) 188
BsaJI CCNNGG 7 cut(s) 273, 279, 546, 711, 719, 774, 821
BsaXI ACNNNNNCTCC 2 cut(s) 53, 83
Bsc4I CCNNNNNNNGG 1 cut(s) 816
Bse1I ACTGG 2 cut(s) 86, 745
BseBI CCWGG 1 cut(s) 281
BseDI CCNNGG 7 cut(s) 273, 279, 546, 711, 719, 774, 821
BseGI GGATG 2 cut(s) 947, 967
BseLI CCNNNNNNNGG 1 cut(s) 816
BseMII CTCAG 1 cut(s) 588
BseNI ACTGG 2 cut(s) 86, 745
BseRI GAGGAG 2 cut(s) 249, 708
BseXI GCAGC 6 cut(s) 360, 567, 742, 961, 978, 983
BshFI GGCC 4 cut(s) 278, 751, 820, 845
BsiHKCI CYCGRG 1 cut(s) 272
BsiSI CCGG 2 cut(s) 578, 846
BslFI GGGAC 1 cut(s) 212
BslI CCNNNNNNNGG 1 cut(s) 816
BsmAI GTCTC 1 cut(s) 188
BsmFI GGGAC 1 cut(s) 212
BsnI GGCC 4 cut(s) 278, 751, 820, 845
Bso31I GGTCTC 1 cut(s) 188
BsoBI CYCGRG 1 cut(s) 272
Bsp143I GATC 4 cut(s) 109, 142, 253, 481
Bsp19I CCATGG 3 cut(s) 546, 711, 774
BspACI CCGC 7 cut(s) 180, 290, 296, 302, 409, 412, 445
BspANI GGCC 4 cut(s) 278, 751, 820, 845
BspCNI CTCAG 1 cut(s) 589
BspLI GGNNCC 3 cut(s) 111, 255, 717
BspMAI CTGCAG 1 cut(s) 760
BspPI GGATC 4 cut(s) 104, 117, 248, 261
BspTI CTTAAG 1 cut(s) 797
BspTNI GGTCTC 1 cut(s) 188
BsrBI CCGCTC 1 cut(s) 447
BsrI ACTGG 2 cut(s) 86, 745
BssECI CCNNGG 7 cut(s) 273, 279, 546, 711, 719, 774, 821
BssMI GATC 4 cut(s) 109, 142, 253, 481
BssT1I CCWWGG 3 cut(s) 546, 711, 774
Bst2UI CCWGG 1 cut(s) 281
Bst4CI ACNGT 6 cut(s) 537, 618, 642, 871, 895, 958
Bst6I CTCTTC 2 cut(s) 661, 809
BstAFI CTTAAG 1 cut(s) 797
BstAPI GCANNNNNTGC 1 cut(s) 538
BstBAI YACGTR 1 cut(s) 627
BstC8I GCNNGC 4 cut(s) 416, 575, 753, 947
BstDEI CTNAG 2 cut(s) 597, 860
BstDSI CCRYGG 3 cut(s) 546, 711, 774
BstF5I GGATG 2 cut(s) 947, 967
BstHHI GCGC 2 cut(s) 405, 996
BstKTI GATC 4 cut(s) 112, 145, 256, 484
BstMAI GTCTC 1 cut(s) 188
BstMBI GATC 4 cut(s) 109, 142, 253, 481
BstMWI GCNNNNNNNGC 3 cut(s) 11, 411, 538
BstNI CCWGG 1 cut(s) 281
BstNSI RCATGY 1 cut(s) 949
BstSCI CCNGG 2 cut(s) 279, 845
BstSFI CTRYAG 1 cut(s) 756
BstV1I GCAGC 6 cut(s) 360, 567, 742, 961, 978, 983
BstX2I RGATCY 2 cut(s) 109, 253
BstYI RGATCY 2 cut(s) 109, 253
BsuI GTATCC 1 cut(s) 698
BsuRI GGCC 4 cut(s) 278, 751, 820, 845
BtgI CCRYGG 3 cut(s) 546, 711, 774
BtsCI GGATG 2 cut(s) 947, 967
BtsIMutI CAGTG 4 cut(s) 647, 752, 867, 891
Cac8I GCNNGC 4 cut(s) 416, 575, 753, 947
CaiI CAGNNNCTG 1 cut(s) 977
CfoI GCGC 2 cut(s) 405, 996
Cfr13I GGNCC 3 cut(s) 277, 567, 907
CseI GACGC 1 cut(s) 333
Csp6I GTAC 3 cut(s) 339, 430, 587
CviAII CATG 6 cut(s) 382, 547, 712, 775, 788, 946
CviQI GTAC 3 cut(s) 339, 430, 587
DdeI CTNAG 2 cut(s) 597, 860
DpnI GATC 4 cut(s) 111, 144, 255, 483
DpnII GATC 4 cut(s) 109, 142, 253, 481
DriI GACNNNNNGTC 1 cut(s) 706
EaeI YGGCCR 2 cut(s) 749, 843
Eam1104I CTCTTC 2 cut(s) 661, 809
Eam1105I GACNNNNNGTC 1 cut(s) 706
EarI CTCTTC 2 cut(s) 661, 809
Eco130I CCWWGG 3 cut(s) 546, 711, 774
Eco31I GGTCTC 1 cut(s) 188
Eco47I GGWCC 2 cut(s) 567, 907
Eco88I CYCGRG 1 cut(s) 272
EcoO109I RGGNCCY 1 cut(s) 277
EcoRI GAATTC 1 cut(s) 999
EcoRII CCWGG 1 cut(s) 279
EcoT14I CCWWGG 3 cut(s) 546, 711, 774
EcoT22I ATGCAT 1 cut(s) 947
ErhI CCWWGG 3 cut(s) 546, 711, 774
FaeI CATG 6 cut(s) 385, 550, 715, 778, 791, 949
FaqI GGGAC 1 cut(s) 212
FatI CATG 6 cut(s) 381, 546, 711, 774, 787, 945
FbaI TGATCA 1 cut(s) 142
FblI GTMKAC 1 cut(s) 322
Fnu4HI GCNGC 7 cut(s) 349, 412, 556, 756, 972, 975, 992
FokI GGATG 2 cut(s) 954, 974
Fsp4HI GCNGC 7 cut(s) 349, 412, 556, 756, 972, 975, 992
FspBI CTAG 2 cut(s) 527, 1011
FspI TGCGCA 1 cut(s) 995
GlaI GCGC 2 cut(s) 404, 995
GluI GCNGC 7 cut(s) 349, 412, 556, 756, 972, 975, 992
HaeIII GGCC 4 cut(s) 278, 751, 820, 845
HapII CCGG 2 cut(s) 578, 846
HgaI GACGC 1 cut(s) 333
HhaI GCGC 2 cut(s) 405, 996
Hin1II CATG 6 cut(s) 385, 550, 715, 778, 791, 949
Hin6I GCGC 2 cut(s) 403, 994
HinP1I GCGC 2 cut(s) 403, 994
HinfI GANTC 1 cut(s) 856
HpaII CCGG 2 cut(s) 578, 846
HphI GGTGA 4 cut(s) 261, 598, 869, 880
Hpy166II GTNNAC 3 cut(s) 323, 697, 781
Hpy188I TCNGA 4 cut(s) 150, 650, 723, 855
Hpy188III TCNNGA 4 cut(s) 116, 140, 185, 1004
Hpy8I GTNNAC 3 cut(s) 323, 697, 781
Hpy99I CGWCG 2 cut(s) 339, 704
HpyAV CCTTC 5 cut(s) 234, 238, 514, 884, 932
HpyCH4III ACNGT 6 cut(s) 537, 618, 642, 871, 895, 958
HpyCH4IV ACGT 1 cut(s) 626
HpyCH4V TGCA 5 cut(s) 235, 541, 632, 758, 945
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 411, 538
HpyF3I CTNAG 2 cut(s) 597, 860
HpySE526I ACGT 1 cut(s) 626
Hsp92II CATG 6 cut(s) 385, 550, 715, 778, 791, 949
HspAI GCGC 2 cut(s) 403, 994
Ksp22I TGATCA 1 cut(s) 142
Kzo9I GATC 4 cut(s) 109, 142, 253, 481
LmnI GCTCC 5 cut(s) 226, 503, 523, 721, 723
Lsp1109I GCAGC 6 cut(s) 360, 567, 742, 961, 978, 983
LweI GCATC 3 cut(s) 444, 497, 932
MaeI CTAG 2 cut(s) 527, 1011
MaeII ACGT 1 cut(s) 626
MaeIII GTNAC 2 cut(s) 769, 886
MalI GATC 4 cut(s) 111, 144, 255, 483
MbiI CCGCTC 1 cut(s) 447
MboI GATC 4 cut(s) 109, 142, 253, 481
MboII GAAGA 9 cut(s) 374, 595, 648, 651, 657, 660, 826, 847, 947
MflI RGATCY 2 cut(s) 109, 253
MlsI TGGCCA 1 cut(s) 751
MluCI AATT 3 cut(s) 193, 492, 999
MluNI TGGCCA 1 cut(s) 751
MlyI GAGTC 1 cut(s) 865
MmeI TCCRAC 1 cut(s) 112
Mox20I TGGCCA 1 cut(s) 751
Mph1103I ATGCAT 1 cut(s) 947
MroXI GAANNNNTTC 1 cut(s) 655
MscI TGGCCA 1 cut(s) 751
MseI TTAA 3 cut(s) 798, 927, 951
MslI CAYNNNNRTG 3 cut(s) 682, 767, 882
Msp20I TGGCCA 1 cut(s) 751
MspA1I CMGCKG 2 cut(s) 755, 974
MspCI CTTAAG 1 cut(s) 797
MspI CCGG 2 cut(s) 578, 846
MspR9I CCNGG 2 cut(s) 281, 847
MvaI CCWGG 1 cut(s) 281
MwoI GCNNNNNNNGC 3 cut(s) 11, 411, 538
NciI CCSGG 1 cut(s) 847
NcoI CCATGG 3 cut(s) 546, 711, 774
NdeII GATC 4 cut(s) 109, 142, 253, 481
NlaIII CATG 6 cut(s) 385, 550, 715, 778, 791, 949
NlaIV GGNNCC 3 cut(s) 111, 255, 717
NmeAIII GCCGAG 1 cut(s) 846
NmuCI GTSAC 2 cut(s) 769, 886
NsbI TGCGCA 1 cut(s) 995
NsiI ATGCAT 1 cut(s) 947
NspI RCATGY 1 cut(s) 949
PaeI GCATGC 1 cut(s) 949
PcsI WCGNNNNNNNCGW 1 cut(s) 331
PdmI GAANNNNTTC 1 cut(s) 655
PkrI GCNGC 7 cut(s) 350, 413, 557, 757, 973, 976, 993
PleI GAGTC 1 cut(s) 864
PpsI GAGTC 1 cut(s) 864
Ppu21I YACGTR 1 cut(s) 627
Psp6I CCWGG 1 cut(s) 279
PspGI CCWGG 1 cut(s) 279
PspN4I GGNNCC 3 cut(s) 111, 255, 717
PspPI GGNCC 3 cut(s) 277, 567, 907
PstI CTGCAG 1 cut(s) 760
PstNI CAGNNNCTG 1 cut(s) 977
PsuI RGATCY 2 cut(s) 109, 253
PvuII CAGCTG 2 cut(s) 755, 974
RsaI GTAC 3 cut(s) 340, 431, 588
RsaNI GTAC 3 cut(s) 339, 430, 587
RseI CAYNNNNRTG 3 cut(s) 682, 767, 882
SaqAI TTAA 3 cut(s) 798, 927, 951
SatI GCNGC 7 cut(s) 349, 412, 556, 756, 972, 975, 992
Sau3AI GATC 4 cut(s) 109, 142, 253, 481
Sau96I GGNCC 3 cut(s) 277, 567, 907
SchI GAGTC 1 cut(s) 865
ScrFI CCNGG 2 cut(s) 281, 847
SfaNI GCATC 3 cut(s) 444, 497, 932
SfcI CTRYAG 1 cut(s) 756
SinI GGWCC 2 cut(s) 567, 907
SmiMI CAYNNNNRTG 3 cut(s) 682, 767, 882
SmlI CTYRAG 2 cut(s) 114, 797
SmoI CTYRAG 2 cut(s) 114, 797
SphI GCATGC 1 cut(s) 949
Sse9I AATT 3 cut(s) 193, 492, 999
SsiI CCGC 7 cut(s) 180, 290, 296, 302, 409, 412, 445
SspMI CTAG 2 cut(s) 527, 1011
StyD4I CCNGG 2 cut(s) 279, 845
StyI CCWWGG 3 cut(s) 546, 711, 774
TaaI ACNGT 6 cut(s) 537, 618, 642, 871, 895, 958
TaiI ACGT 1 cut(s) 629
TaqI TCGA 1 cut(s) 186
TasI AATT 3 cut(s) 193, 492, 999
TauI GCSGC 1 cut(s) 414
Tru1I TTAA 3 cut(s) 798, 927, 951
Tru9I TTAA 3 cut(s) 798, 927, 951
TscAI CASTG 4 cut(s) 647, 752, 874, 898
TseFI GTSAC 2 cut(s) 769, 886
TseI GCWGC 6 cut(s) 348, 555, 755, 971, 974, 991
Tsp45I GTSAC 2 cut(s) 769, 886
TspDTI ATGAA 8 cut(s) 204, 216, 437, 651, 660, 915, 978, 999
TspRI CASTG 4 cut(s) 647, 752, 874, 898
Vha464I CTTAAG 1 cut(s) 797
VpaK11BI GGWCC 2 cut(s) 567, 907
XapI RAATTY 1 cut(s) 999
XceI RCATGY 1 cut(s) 949
XmiI GTMKAC 1 cut(s) 322
XmnI GAANNNNTTC 1 cut(s) 655
XspI CTAG 2 cut(s) 527, 1011
Zsp2I ATGCAT 1 cut(s) 947
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.