pycom12g20890

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002598
Physical Location & Seq
Forward (+)
22237402 .. 22238722
1321 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g20890.1

Sequence Viewer

Length: 975 bp
ATGGCTCTGGAAGCCGTCGTTTTCCCTCAAGACCCATTTAGTTACACTACTAACAAAGACCTCTACAACTTGTTAGGAGGAAATTTGAGCTATGTTGATGATCAACAGCAAGCCTCTCTTGATTTTCTCGACTACCAAACAGACCAGAATTACCCTAACTATGGTTCTACGTATTCCTCACCGCCTTCAATGGTACCTCACTTCAACGAATTGCACCTCTCCAATCCAAACCCAGTTGCCAGCACTACAAACCCTACTGGTGATCAACCCGAATTTCAACTACCACTGGAGGACACAATCAACATGTCTAGAAGTTCTCGTGTGAAACGACGTCGTGCGAAGAGTAAAAAGAACGAGGAGGAGATTGAGAACCAGAGAATGACTCACATCGCAGTTGAGCGCAACAGGAGAAAACAGATGAATGAGTATCTCTCTGTGCTTCGATCGATAATGCCCGACTCGTACGTCCAAAGGGGAGACCAAGCATCAATCATTGGGGGTGCGATTAATTATGTGAAGGAGCTAGAGCAAGAAGTGCAATTCCTGGGCGTCCAAAAGACAAATGATCGTGCGCCTTTTTCCGAATTCTTCACCTTCCCCCAGTACTCATCGAGGTCATCTACTAGTGATCATGAGTCTGCGGTGGCAGCCATGGCGGAGCTGCCGTTGCTCGAGTGCAGGTCGAGCAAAATTGCAGCTGACATAGAAGTGACAATGGTGGAGAGCCATGCAAGCCTTAAAGTAAGATCCAAAAAGGTCCCAAAGCAACTCTTGAAAATTGTTTCAGGGTTGCATGATATGCACCTTACCATTCTCCATCTCAATGTTGTCACTGTTGATGATATTGTCCTCTATTCTCTCAGTCTCAAGCAGGTAGAAGATGAGTGCATGCTGACTTCAGTGGATGAGATTGCAACAGCTGTCCATGAGATGCTAGCTAGGATTCAGGAAGAGGCAATGCTGAATTTGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

325

Amino Acids

36.67

Weight (kDa)

5.18

Isoelectric Point (pI)

58.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 125 - 176 3.8e-13 Helix-loop-helix DNA-binding domain
bHLH-TF_ACT-like_plant PF22754 234 - 311 9.2e-06 Plant bHLH transcription factor, ACT-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 464
AatII GACGTC 1 cut(s) 334
Acc36I ACCTGC 2 cut(s) 669, 862
Acc65I GGTACC 1 cut(s) 193
AccB1I GGYRCC 1 cut(s) 193
AciI CCGC 3 cut(s) 182, 641, 656
AclWI GGATC 1 cut(s) 741
AcsI RAATTY 4 cut(s) 82, 272, 584, 964
AcuI CTGAAG 1 cut(s) 882
AcyI GRCGYC 2 cut(s) 331, 549
AfaI GTAC 3 cut(s) 195, 464, 605
AfiI CCNNNNNNNGG 1 cut(s) 161
AflIII ACRYGT 1 cut(s) 303
AgsI TTSAA 5 cut(s) 189, 205, 278, 775, 970
AhlI ACTAGT 1 cut(s) 623
AjnI CCWGG 1 cut(s) 543
AluBI AGCT 6 cut(s) 90, 523, 661, 698, 920, 938
AluI AGCT 6 cut(s) 90, 523, 661, 698, 920, 938
Alw26I GTCTC 2 cut(s) 471, 869
AlwI GGATC 1 cut(s) 741
Ama87I CYCGRG 1 cut(s) 671
ApeKI GCWGC 3 cut(s) 647, 661, 695
ApoI RAATTY 4 cut(s) 82, 272, 584, 964
AseI ATTAAT 1 cut(s) 507
Asp718I GGTACC 1 cut(s) 193
AspLEI GCGC 2 cut(s) 402, 574
AspS9I GGNCC 1 cut(s) 757
AsuHPI GGTGA 3 cut(s) 171, 272, 583
AsuNHI GCTAGC 1 cut(s) 934
AvaI CYCGRG 1 cut(s) 671
AvaII GGWCC 1 cut(s) 757
BanI GGYRCC 1 cut(s) 193
BauI CACGAG 1 cut(s) 318
BbvI GCAGC 3 cut(s) 648, 659, 707
BccI CCATC 1 cut(s) 825
BceAI ACGGC 1 cut(s) 649
BciT130I CCWGG 1 cut(s) 545
BclI TGATCA 3 cut(s) 100, 262, 628
BcoDI GTCTC 2 cut(s) 471, 869
BcuI ACTAGT 1 cut(s) 623
BfaI CTAG 5 cut(s) 309, 524, 624, 935, 939
BfuAI ACCTGC 2 cut(s) 669, 862
BisI GCNGC 3 cut(s) 648, 662, 696
BlsI GCNGC 3 cut(s) 649, 663, 697
BmcAI AGTACT 1 cut(s) 605
Bme1390I CCNGG 1 cut(s) 545
Bme18I GGWCC 1 cut(s) 757
BmeT110I CYCGRG 1 cut(s) 671
BmgT120I GGNCC 1 cut(s) 757
BmiI GGNNCC 2 cut(s) 195, 759
BmrFI CCNGG 1 cut(s) 545
BmrI ACTGGG 2 cut(s) 227, 595
BmsI GCATC 2 cut(s) 494, 921
BmtI GCTAGC 1 cut(s) 938
BmuI ACTGGG 2 cut(s) 227, 595
BplI GAGNNNNNCTC 4 cut(s) 367, 399, 416, 448
BpmI CTGGAG 1 cut(s) 308
BpuEI CTTGAG 2 cut(s) 12, 851
Bsa29I ATCGAT 1 cut(s) 446
BsaAI YACGTR 1 cut(s) 171
BsaHI GRCGYC 2 cut(s) 331, 549
BsaI GGTCTC 1 cut(s) 471
BsaJI CCNNGG 2 cut(s) 544, 651
BsaXI ACNNNNNCTCC 2 cut(s) 650, 680
Bsc4I CCNNNNNNNGG 1 cut(s) 161
Bse1I ACTGG 4 cut(s) 233, 262, 291, 601
Bse3DI GCAATG 1 cut(s) 963
BseBI CCWGG 1 cut(s) 545
BseCI ATCGAT 1 cut(s) 446
BseDI CCNNGG 2 cut(s) 544, 651
BseGI GGATG 1 cut(s) 910
BseLI CCNNNNNNNGG 1 cut(s) 161
BseMI GCAATG 1 cut(s) 963
BseMII CTCAG 1 cut(s) 874
BseNI ACTGG 4 cut(s) 233, 262, 291, 601
BseRI GAGGAG 2 cut(s) 371, 374
BseXI GCAGC 3 cut(s) 648, 659, 707
BsgI GTGCAG 1 cut(s) 697
Bsh1285I CGRYCG 1 cut(s) 446
BshNI GGYRCC 1 cut(s) 193
BshVI ATCGAT 1 cut(s) 446
BsiEI CGRYCG 1 cut(s) 446
BsiHKCI CYCGRG 1 cut(s) 671
BsiWI CGTACG 1 cut(s) 462
BslFI GGGAC 1 cut(s) 743
BslI CCNNNNNNNGG 1 cut(s) 161
BsmAI GTCTC 2 cut(s) 471, 869
BsmFI GGGAC 1 cut(s) 743
Bso31I GGTCTC 1 cut(s) 471
BsoBI CYCGRG 1 cut(s) 671
Bsp143I GATC 6 cut(s) 100, 262, 443, 565, 628, 746
Bsp19I CCATGG 1 cut(s) 651
BspACI CCGC 3 cut(s) 182, 641, 656
BspCNI CTCAG 1 cut(s) 873
BspDI ATCGAT 1 cut(s) 446
BspHI TCATGA 1 cut(s) 631
BspLI GGNNCC 2 cut(s) 195, 759
BspMI ACCTGC 2 cut(s) 669, 862
BspOI GCTAGC 1 cut(s) 938
BspPI GGATC 1 cut(s) 741
BspT107I GGYRCC 1 cut(s) 193
BspTNI GGTCTC 1 cut(s) 471
BsrDI GCAATG 1 cut(s) 963
BsrI ACTGG 4 cut(s) 233, 262, 291, 601
BssECI CCNNGG 2 cut(s) 544, 651
BssMI GATC 6 cut(s) 100, 262, 443, 565, 628, 746
BssNI GRCGYC 2 cut(s) 331, 549
BssSI CACGAG 1 cut(s) 318
BssT1I CCWWGG 1 cut(s) 651
Bst2BI CACGAG 1 cut(s) 318
Bst2UI CCWGG 1 cut(s) 545
Bst4CI ACNGT 1 cut(s) 835
Bst6I CTCTTC 2 cut(s) 335, 945
BstACI GRCGYC 2 cut(s) 331, 549
BstAPI GCANNNNNTGC 2 cut(s) 535, 799
BstBAI YACGTR 1 cut(s) 171
BstC8I GCNNGC 5 cut(s) 111, 241, 733, 890, 936
BstDEI CTNAG 1 cut(s) 860
BstDSI CCRYGG 1 cut(s) 651
BstF5I GGATG 1 cut(s) 910
BstHHI GCGC 2 cut(s) 402, 574
BstKTI GATC 6 cut(s) 103, 265, 446, 568, 631, 749
BstMAI GTCTC 2 cut(s) 471, 869
BstMBI GATC 6 cut(s) 100, 262, 443, 565, 628, 746
BstMCI CGRYCG 1 cut(s) 446
BstMWI GCNNNNNNNGC 8 cut(s) 11, 535, 647, 653, 667, 684, 732, 799
BstNI CCWGG 1 cut(s) 545
BstNSI RCATGY 2 cut(s) 307, 892
BstSCI CCNGG 1 cut(s) 543
BstSNI TACGTA 1 cut(s) 171
BstV1I GCAGC 3 cut(s) 648, 659, 707
BstX2I RGATCY 1 cut(s) 746
BstYI RGATCY 1 cut(s) 746
Bsu15I ATCGAT 1 cut(s) 446
BsuTUI ATCGAT 1 cut(s) 446
BtgI CCRYGG 1 cut(s) 651
BtgZI GCGATG 1 cut(s) 373
BtsCI GGATG 1 cut(s) 910
BtsIMutI CAGTG 3 cut(s) 284, 831, 906
BveI ACCTGC 2 cut(s) 669, 862
Cac8I GCNNGC 5 cut(s) 111, 241, 733, 890, 936
CciI TCATGA 1 cut(s) 631
CfoI GCGC 2 cut(s) 402, 574
Cfr13I GGNCC 1 cut(s) 757
ClaI ATCGAT 1 cut(s) 446
CseI GACGC 1 cut(s) 538
Csp6I GTAC 3 cut(s) 194, 463, 604
CviAII CATG 7 cut(s) 304, 632, 652, 728, 794, 889, 926
CviQI GTAC 3 cut(s) 194, 463, 604
DdeI CTNAG 1 cut(s) 860
DpnI GATC 6 cut(s) 102, 264, 445, 567, 630, 748
DpnII GATC 6 cut(s) 100, 262, 443, 565, 628, 746
DrdI GACNNNNNNGTC 1 cut(s) 464
DseDI GACNNNNNNGTC 1 cut(s) 464
Eam1104I CTCTTC 2 cut(s) 335, 945
EarI CTCTTC 2 cut(s) 335, 945
EciI GGCGGA 1 cut(s) 671
Eco105I TACGTA 1 cut(s) 171
Eco130I CCWWGG 1 cut(s) 651
Eco31I GGTCTC 1 cut(s) 471
Eco47I GGWCC 1 cut(s) 757
Eco57I CTGAAG 1 cut(s) 882
Eco88I CYCGRG 1 cut(s) 671
EcoO109I RGGNCCY 1 cut(s) 757
EcoRI GAATTC 1 cut(s) 584
EcoRII CCWGG 1 cut(s) 543
EcoT14I CCWWGG 1 cut(s) 651
ErhI CCWWGG 1 cut(s) 651
FaeI CATG 7 cut(s) 307, 635, 655, 731, 797, 892, 929
FalI AAGNNNNNCTT 4 cut(s) 102, 134, 755, 787
FaqI GGGAC 1 cut(s) 743
FatI CATG 7 cut(s) 303, 631, 651, 727, 793, 888, 925
FbaI TGATCA 3 cut(s) 100, 262, 628
Fnu4HI GCNGC 3 cut(s) 648, 662, 696
FokI GGATG 1 cut(s) 917
Fsp4HI GCNGC 3 cut(s) 648, 662, 696
FspBI CTAG 5 cut(s) 309, 524, 624, 935, 939
GlaI GCGC 2 cut(s) 401, 573
GluI GCNGC 3 cut(s) 648, 662, 696
GsuI CTGGAG 1 cut(s) 308
HgaI GACGC 1 cut(s) 538
HhaI GCGC 2 cut(s) 402, 574
Hin1I GRCGYC 2 cut(s) 331, 549
Hin1II CATG 7 cut(s) 307, 635, 655, 731, 797, 892, 929
Hin6I GCGC 2 cut(s) 400, 572
HinP1I GCGC 2 cut(s) 400, 572
HinfI GANTC 4 cut(s) 382, 458, 635, 943
HphI GGTGA 3 cut(s) 171, 272, 583
Hpy188I TCNGA 1 cut(s) 583
Hpy188III TCNNGA 8 cut(s) 8, 29, 119, 128, 309, 632, 772, 947
Hpy99I CGWCG 3 cut(s) 20, 333, 336
HpyAV CCTTC 3 cut(s) 195, 511, 604
HpyCH4III ACNGT 1 cut(s) 835
HpyCH4IV ACGT 3 cut(s) 170, 331, 465
HpyCH4V TGCA 9 cut(s) 214, 538, 678, 695, 731, 793, 802, 888, 914
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 535, 647, 653, 667, 684, 732, 799
HpyF3I CTNAG 1 cut(s) 860
HpySE526I ACGT 3 cut(s) 170, 331, 465
Hsp92I GRCGYC 2 cut(s) 331, 549
Hsp92II CATG 7 cut(s) 307, 635, 655, 731, 797, 892, 929
HspAI GCGC 2 cut(s) 400, 572
KpnI GGTACC 1 cut(s) 197
Ksp22I TGATCA 3 cut(s) 100, 262, 628
Kzo9I GATC 6 cut(s) 100, 262, 443, 565, 628, 746
LmnI GCTCC 2 cut(s) 520, 658
Lsp1109I GCAGC 3 cut(s) 648, 659, 707
LweI GCATC 2 cut(s) 494, 921
MaeI CTAG 5 cut(s) 309, 524, 624, 935, 939
MaeII ACGT 3 cut(s) 170, 331, 465
MaeIII GTNAC 3 cut(s) 41, 709, 829
MalI GATC 6 cut(s) 102, 264, 445, 567, 630, 748
MboI GATC 6 cut(s) 100, 262, 443, 565, 628, 746
MboII GAAGA 4 cut(s) 352, 580, 890, 962
MflI RGATCY 1 cut(s) 746
MlyI GAGTC 3 cut(s) 376, 452, 644
MseI TTAA 2 cut(s) 507, 738
MslI CAYNNNNRTG 2 cut(s) 707, 822
MspA1I CMGCKG 2 cut(s) 698, 920
MspR9I CCNGG 1 cut(s) 545
MvaI CCWGG 1 cut(s) 545
MwoI GCNNNNNNNGC 8 cut(s) 11, 535, 647, 653, 667, 684, 732, 799
NcoI CCATGG 1 cut(s) 651
NdeII GATC 6 cut(s) 100, 262, 443, 565, 628, 746
NheI GCTAGC 1 cut(s) 934
NlaIII CATG 7 cut(s) 307, 635, 655, 731, 797, 892, 929
NlaIV GGNNCC 2 cut(s) 195, 759
NmuCI GTSAC 2 cut(s) 709, 829
NspI RCATGY 2 cut(s) 307, 892
PaeI GCATGC 1 cut(s) 892
PaeR7I CTCGAG 1 cut(s) 671
PagI TCATGA 1 cut(s) 631
PciI ACATGT 1 cut(s) 303
PcsI WCGNNNNNNNCGW 1 cut(s) 325
PfeI GAWTC 1 cut(s) 943
Pfl23II CGTACG 1 cut(s) 462
PkrI GCNGC 3 cut(s) 649, 663, 697
Ple19I CGATCG 1 cut(s) 446
PleI GAGTC 3 cut(s) 376, 452, 643
PpsI GAGTC 3 cut(s) 376, 452, 643
Ppu21I YACGTR 1 cut(s) 171
PpuMI RGGWCCY 1 cut(s) 757
PscI ACATGT 1 cut(s) 303
PshBI ATTAAT 1 cut(s) 507
Psp5II RGGWCCY 1 cut(s) 757
Psp6I CCWGG 1 cut(s) 543
PspGI CCWGG 1 cut(s) 543
PspLI CGTACG 1 cut(s) 462
PspN4I GGNNCC 2 cut(s) 195, 759
PspPI GGNCC 1 cut(s) 757
PspPPI RGGWCCY 1 cut(s) 757
PspXI VCTCGAGB 1 cut(s) 671
PsuI RGATCY 1 cut(s) 746
PvuI CGATCG 1 cut(s) 446
PvuII CAGCTG 2 cut(s) 698, 920
RsaI GTAC 3 cut(s) 195, 464, 605
RsaNI GTAC 3 cut(s) 194, 463, 604
RseI CAYNNNNRTG 2 cut(s) 707, 822
SaqAI TTAA 2 cut(s) 507, 738
SatI GCNGC 3 cut(s) 648, 662, 696
Sau3AI GATC 6 cut(s) 100, 262, 443, 565, 628, 746
Sau96I GGNCC 1 cut(s) 757
ScaI AGTACT 1 cut(s) 605
SchI GAGTC 3 cut(s) 376, 452, 644
ScrFI CCNGG 1 cut(s) 545
SfaNI GCATC 2 cut(s) 494, 921
Sfr274I CTCGAG 1 cut(s) 671
SinI GGWCC 1 cut(s) 757
SlaI CTCGAG 1 cut(s) 671
SmiMI CAYNNNNRTG 2 cut(s) 707, 822
SmlI CTYRAG 3 cut(s) 27, 671, 866
SmoI CTYRAG 3 cut(s) 27, 671, 866
SnaBI TACGTA 1 cut(s) 171
SpeI ACTAGT 1 cut(s) 623
SphI GCATGC 1 cut(s) 892
SsiI CCGC 3 cut(s) 182, 641, 656
SspMI CTAG 5 cut(s) 309, 524, 624, 935, 939
StyD4I CCNGG 1 cut(s) 543
StyI CCWWGG 1 cut(s) 651
TaaI ACNGT 1 cut(s) 835
TaiI ACGT 3 cut(s) 173, 334, 468
TaqI TCGA 6 cut(s) 129, 442, 446, 611, 672, 683
TatI WGTACW 1 cut(s) 603
TfiI GAWTC 1 cut(s) 943
Tru1I TTAA 2 cut(s) 507, 738
Tru9I TTAA 2 cut(s) 507, 738
TscAI CASTG 3 cut(s) 291, 838, 906
TseFI GTSAC 2 cut(s) 709, 829
TseI GCWGC 3 cut(s) 647, 661, 695
Tsp45I GTSAC 2 cut(s) 709, 829
TspDTI ATGAA 1 cut(s) 434
TspRI CASTG 3 cut(s) 291, 838, 906
VpaK11BI GGWCC 1 cut(s) 757
VspI ATTAAT 1 cut(s) 507
XapI RAATTY 4 cut(s) 82, 272, 584, 964
XbaI TCTAGA 1 cut(s) 308
XceI RCATGY 2 cut(s) 307, 892
XhoI CTCGAG 1 cut(s) 671
XspI CTAG 5 cut(s) 309, 524, 624, 935, 939
ZraI GACGTC 1 cut(s) 332
ZrmI AGTACT 1 cut(s) 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.