FvH4_6g14711

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005680
Physical Location & Seq
Forward (+)
9076926 .. 9077428
503 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g14711.t1

Sequence Viewer

Length: 468 bp
ATGAAACATGAAGAAGCATGGAGCTGCAGTGCCCAACTCGGAACCACAATGAAACTGAAACTGAAGCTGAGACGAAATTTGCAGACCGGCCAAGATGCCCCACAAGAGACCAAGAAAATCGAATTGATCGGGTCGTCGCCAAAAGTTTACGTGGGTACCTCATCCCCAGTTGGCGACCAGATTCAGAGGCAAGCTGATGCACAAGCTTCTGAAACTCTGATCAAGAACTTTGATCAGCTTCCACCTCTGCTTCTAACCCAATCGATGTTCAACGATTCTGGTGATGAAGAATGGCTCTTTGAGAAGAAGCAGAATCAGCAGACTGAGCAATCTTCTTCTTCTAAAAGATTTAAAGATAACCAGGATGAACCCATGGCCGGTTGTGCAATTCGGACTTTGTGGCCACCAAAAGCACAATGGTTGCCTCAGGCTGGCATATACGCCTCGCCGTTCACCGTTCCACTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

156

Amino Acids

17.41

Weight (kDa)

6.59

Isoelectric Point (pI)

68.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 155
AccB1I GGYRCC 1 cut(s) 155
AcoI YGGCCR 3 cut(s) 88, 375, 401
AcsI RAATTY 1 cut(s) 76
AcuI CTGAAG 1 cut(s) 83
AfaI GTAC 1 cut(s) 157
AfiI CCNNNNNNNGG 2 cut(s) 377, 431
AgsI TTSAA 1 cut(s) 271
AjnI CCWGG 1 cut(s) 360
AluBI AGCT 5 cut(s) 24, 67, 194, 206, 238
AluI AGCT 5 cut(s) 24, 67, 194, 206, 238
Alw26I GTCTC 2 cut(s) 64, 101
AoxI GGCC 3 cut(s) 88, 375, 401
ApeKI GCWGC 1 cut(s) 24
ApoI RAATTY 1 cut(s) 76
Asp718I GGTACC 1 cut(s) 155
AsuHPI GGTGA 2 cut(s) 293, 445
AxyI CCTNAGG 1 cut(s) 426
BaeGI GKGCMC 1 cut(s) 34
BaeI ACNNNNGTAYC 2 cut(s) 139, 172
BalI TGGCCA 1 cut(s) 403
BanI GGYRCC 1 cut(s) 155
BbvI GCAGC 1 cut(s) 11
BceAI ACGGC 1 cut(s) 433
BciT130I CCWGG 1 cut(s) 362
BclI TGATCA 2 cut(s) 219, 232
BcoDI GTCTC 2 cut(s) 64, 101
BfmI CTRYAG 1 cut(s) 25
BisI GCNGC 1 cut(s) 25
BlsI GCNGC 1 cut(s) 26
Bme1390I CCNGG 1 cut(s) 362
BmiI GGNNCC 2 cut(s) 43, 157
BmrFI CCNGG 1 cut(s) 362
BmrI ACTGGG 1 cut(s) 161
BmsI GCATC 2 cut(s) 85, 187
BmuI ACTGGG 1 cut(s) 161
Bsa29I ATCGAT 1 cut(s) 263
BsaAI YACGTR 1 cut(s) 151
BsaI GGTCTC 1 cut(s) 101
BsaJI CCNNGG 1 cut(s) 372
BsaXI ACNNNNNCTCC 2 cut(s) 13, 43
Bsc4I CCNNNNNNNGG 2 cut(s) 377, 431
Bse118I RCCGGY 2 cut(s) 86, 377
Bse1I ACTGG 1 cut(s) 167
Bse21I CCTNAGG 1 cut(s) 426
BseBI CCWGG 1 cut(s) 362
BseCI ATCGAT 1 cut(s) 263
BseDI CCNNGG 1 cut(s) 372
BseGI GGATG 2 cut(s) 161, 370
BseLI CCNNNNNNNGG 2 cut(s) 377, 431
BseMII CTCAG 3 cut(s) 59, 315, 440
BseNI ACTGG 1 cut(s) 167
BseSI GKGCMC 1 cut(s) 34
BseXI GCAGC 1 cut(s) 11
BshFI GGCC 3 cut(s) 90, 377, 403
BshNI GGYRCC 1 cut(s) 155
BshVI ATCGAT 1 cut(s) 263
BsiSI CCGG 2 cut(s) 87, 378
BslI CCNNNNNNNGG 2 cut(s) 377, 431
BsmAI GTCTC 2 cut(s) 64, 101
BsmBI CGTCTC 1 cut(s) 64
BsnI GGCC 3 cut(s) 90, 377, 403
Bso31I GGTCTC 1 cut(s) 101
Bsp1286I GDGCHC 1 cut(s) 34
Bsp143I GATC 3 cut(s) 126, 219, 232
Bsp19I CCATGG 1 cut(s) 372
BspANI GGCC 3 cut(s) 90, 377, 403
BspCNI CTCAG 3 cut(s) 60, 316, 439
BspDI ATCGAT 1 cut(s) 263
BspLI GGNNCC 2 cut(s) 43, 157
BspMAI CTGCAG 1 cut(s) 29
BspT107I GGYRCC 1 cut(s) 155
BspTNI GGTCTC 1 cut(s) 101
BsrFI RCCGGY 2 cut(s) 86, 377
BsrI ACTGG 1 cut(s) 167
BssAI RCCGGY 2 cut(s) 86, 377
BssECI CCNNGG 1 cut(s) 372
BssMI GATC 3 cut(s) 126, 219, 232
BssT1I CCWWGG 1 cut(s) 372
Bst2UI CCWGG 1 cut(s) 362
Bst4CI ACNGT 1 cut(s) 457
BstBAI YACGTR 1 cut(s) 151
BstC8I GCNNGC 2 cut(s) 192, 433
BstDEI CTNAG 3 cut(s) 68, 324, 426
BstDSI CCRYGG 1 cut(s) 372
BstF5I GGATG 2 cut(s) 161, 370
BstKTI GATC 3 cut(s) 129, 222, 235
BstMAI GTCTC 2 cut(s) 64, 101
BstMBI GATC 3 cut(s) 126, 219, 232
BstMWI GCNNNNNNNGC 3 cut(s) 316, 325, 383
BstNI CCWGG 1 cut(s) 362
BstSCI CCNGG 1 cut(s) 360
BstSFI CTRYAG 1 cut(s) 25
BstSLI GKGCMC 1 cut(s) 34
BstV1I GCAGC 1 cut(s) 11
Bsu15I ATCGAT 1 cut(s) 263
Bsu36I CCTNAGG 1 cut(s) 426
BsuRI GGCC 3 cut(s) 90, 377, 403
BsuTUI ATCGAT 1 cut(s) 263
BtgI CCRYGG 1 cut(s) 372
BtsCI GGATG 2 cut(s) 161, 370
BtsI GCAGTG 1 cut(s) 34
BtsIMutI CAGTG 1 cut(s) 34
Cac8I GCNNGC 2 cut(s) 192, 433
Cfr10I RCCGGY 2 cut(s) 86, 377
ClaI ATCGAT 1 cut(s) 263
Csp6I GTAC 1 cut(s) 156
CviAII CATG 3 cut(s) 8, 18, 373
CviQI GTAC 1 cut(s) 156
DdeI CTNAG 3 cut(s) 68, 324, 426
DpnI GATC 3 cut(s) 128, 221, 234
DpnII GATC 3 cut(s) 126, 219, 232
DraI TTTAAA 1 cut(s) 352
EaeI YGGCCR 3 cut(s) 88, 375, 401
Eco130I CCWWGG 1 cut(s) 372
Eco31I GGTCTC 1 cut(s) 101
Eco57I CTGAAG 1 cut(s) 83
Eco81I CCTNAGG 1 cut(s) 426
EcoRII CCWGG 1 cut(s) 360
EcoT14I CCWWGG 1 cut(s) 372
ErhI CCWWGG 1 cut(s) 372
Esp3I CGTCTC 1 cut(s) 64
FaeI CATG 3 cut(s) 11, 21, 376
FaiI YATR 5 cut(s) 9, 19, 374, 437, 439
FatI CATG 3 cut(s) 7, 17, 372
FbaI TGATCA 2 cut(s) 219, 232
Fnu4HI GCNGC 1 cut(s) 25
FokI GGATG 2 cut(s) 148, 377
Fsp4HI GCNGC 1 cut(s) 25
GluI GCNGC 1 cut(s) 25
HaeIII GGCC 3 cut(s) 90, 377, 403
HapII CCGG 2 cut(s) 87, 378
Hin1II CATG 3 cut(s) 11, 21, 376
HindIII AAGCTT 1 cut(s) 204
HinfI GANTC 3 cut(s) 181, 275, 313
HpaII CCGG 2 cut(s) 87, 378
HphI GGTGA 2 cut(s) 293, 445
Hpy166II GTNNAC 2 cut(s) 148, 453
Hpy188I TCNGA 5 cut(s) 41, 186, 211, 219, 393
Hpy188III TCNNGA 1 cut(s) 223
Hpy8I GTNNAC 2 cut(s) 148, 453
Hpy99I CGWCG 1 cut(s) 139
HpyCH4III ACNGT 1 cut(s) 457
HpyCH4IV ACGT 1 cut(s) 150
HpyCH4V TGCA 4 cut(s) 27, 82, 200, 386
HpyF10VI GCNNNNNNNGC 3 cut(s) 316, 325, 383
HpyF3I CTNAG 3 cut(s) 68, 324, 426
HpySE526I ACGT 1 cut(s) 150
Hsp92II CATG 3 cut(s) 11, 21, 376
KpnI GGTACC 1 cut(s) 159
Ksp22I TGATCA 2 cut(s) 219, 232
Kzo9I GATC 3 cut(s) 126, 219, 232
LmnI GCTCC 1 cut(s) 21
LpnPI CCDG 9 cut(s) 100, 180, 191, 264, 347, 374, 391, 413, 417
Lsp1109I GCAGC 1 cut(s) 11
LweI GCATC 2 cut(s) 85, 187
MaeII ACGT 1 cut(s) 150
MalI GATC 3 cut(s) 128, 221, 234
MboI GATC 3 cut(s) 126, 219, 232
MboII GAAGA 6 cut(s) 23, 299, 316, 324, 327, 330
MhlI GDGCHC 1 cut(s) 34
MlsI TGGCCA 1 cut(s) 403
MluCI AATT 3 cut(s) 76, 122, 387
MluNI TGGCCA 1 cut(s) 403
MnlI CCTC 5 cut(s) 169, 180, 255, 435, 454
Mox20I TGGCCA 1 cut(s) 403
MscI TGGCCA 1 cut(s) 403
MseI TTAA 1 cut(s) 351
Msp20I TGGCCA 1 cut(s) 403
MspI CCGG 2 cut(s) 87, 378
MspR9I CCNGG 1 cut(s) 362
MvaI CCWGG 1 cut(s) 362
MwoI GCNNNNNNNGC 3 cut(s) 316, 325, 383
NcoI CCATGG 1 cut(s) 372
NdeII GATC 3 cut(s) 126, 219, 232
NlaIII CATG 3 cut(s) 11, 21, 376
NlaIV GGNNCC 2 cut(s) 43, 157
PfeI GAWTC 3 cut(s) 181, 275, 313
PkrI GCNGC 1 cut(s) 26
Ppu21I YACGTR 1 cut(s) 151
Psp6I CCWGG 1 cut(s) 360
PspGI CCWGG 1 cut(s) 360
PspN4I GGNNCC 2 cut(s) 43, 157
PstI CTGCAG 1 cut(s) 29
RsaI GTAC 1 cut(s) 157
RsaNI GTAC 1 cut(s) 156
SaqAI TTAA 1 cut(s) 351
SatI GCNGC 1 cut(s) 25
Sau3AI GATC 3 cut(s) 126, 219, 232
ScrFI CCNGG 1 cut(s) 362
SduI GDGCHC 1 cut(s) 34
SetI ASST 8 cut(s) 26, 69, 153, 161, 196, 208, 240, 247
SfaNI GCATC 2 cut(s) 85, 187
SfcI CTRYAG 1 cut(s) 25
Sse9I AATT 3 cut(s) 76, 122, 387
StyD4I CCNGG 1 cut(s) 360
StyI CCWWGG 1 cut(s) 372
TaaI ACNGT 1 cut(s) 457
TaiI ACGT 1 cut(s) 153
TaqI TCGA 2 cut(s) 120, 263
TasI AATT 3 cut(s) 76, 122, 387
TfiI GAWTC 3 cut(s) 181, 275, 313
Tru1I TTAA 1 cut(s) 351
Tru9I TTAA 1 cut(s) 351
TscAI CASTG 1 cut(s) 34
TseI GCWGC 1 cut(s) 24
TspDTI ATGAA 5 cut(s) 17, 24, 65, 300, 381
TspRI CASTG 1 cut(s) 34
XapI RAATTY 1 cut(s) 76
XcmI CCANNNNNNNNNTGG 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.