FvH4_6g16910

expansin-A9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004238
Physical Location & Seq
Reverse (-)
10791126 .. 10792423
1298 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g16910.t1

Sequence Viewer

Length: 789 bp
ATGTCGATGGCGTCCATAAGTTCTGCCCTCTCGGGTTCATTGCTTCTCATTCTTCTCGTACTACCTGACAATACTAATGCTCACCATAGGCCAAGTTTCAGACCTGGCCCTTGGAAGCATGCTCATGCTACATTTTACGAAGGCGGCTCTGGATCATTTGGTGGTGCTTGTGGTTACCACGACGTCGTTCAAGAAGGCTATGGCCTCGACACAGTGGCTTTGAGCGACACCTTGTTCAACAATGGTCAGTCTTGTGGTGCATGCTACGACATCCAATGTGTGGACTCCCCACAATGGTGCAAGCCCGGGCAGCCAACTCTTCATGTTACAGCAACCAACCATTGCCCCCCGAACTATAACCTTCCGAGTGACAATGGAGGATGGTGCAATGCACCACGCGAGCACTTCGATATAGCCAAGCCAGTGTTCCTCAACATTGCTGAGTACAAGGCCGGCATAGTTCCAATCATATACCGCAGGATTCCATGCCAAAAGAGAGGAGGCATCAGATTCACCATAACTGGGAATCCTTACTACAACGAAGTGTTGGTGTGGAACGTGGGAGGTGCCGGTGATGTGATCAGCGTGCAAGTGAAGGGCGACGACAAGCTGAAATGGACAACGATGAACCGAATGTGGGGTCAGAGGTGGATTACTGATGCAAGGCTGGTTGGAGAGTCTCTGACCTTCAGAGTTGAAGCAAGTGATAAAAGACGCTCGACTTCATGGCATATTGCCCCTAAGAACTGGCAATTTGGCCAGACCTTTGAAGGCAAGAACTTCAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

263

Amino Acids

29.08

Weight (kDa)

8.6

Isoelectric Point (pI)

38.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXPB1_D1 PF22514 43 - 141 1.9e-06 EXPB1-like domain 1
DPBB_1 PF03330 71 - 158 9.7e-18 Lytic transglycolase
Expansin_C PF01357 169 - 247 8e-24 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 186
AccB1I GGYRCC 1 cut(s) 566
AccB7I CCANNNNNTGG 1 cut(s) 280
AccII CGCG 1 cut(s) 399
AciI CCGC 2 cut(s) 144, 475
AclWI GGATC 1 cut(s) 160
AcoI YGGCCR 1 cut(s) 757
AcuI CTGAAG 2 cut(s) 673, 766
AcyI GRCGYC 2 cut(s) 11, 183
AfaI GTAC 2 cut(s) 60, 446
AfiI CCNNNNNNNGG 4 cut(s) 280, 294, 522, 637
AgsI TTSAA 4 cut(s) 191, 238, 698, 770
AjnI CCWGG 1 cut(s) 103
AjuI GAANNNNNNNTTGG 2 cut(s) 410, 442
AleI CACNNNNGTG 1 cut(s) 295
AluBI AGCT 1 cut(s) 610
AluI AGCT 1 cut(s) 610
Alw21I GWGCWC 1 cut(s) 405
Alw26I GTCTC 1 cut(s) 684
AlwI GGATC 1 cut(s) 160
Ama87I CYCGRG 2 cut(s) 31, 305
AoxI GGCC 5 cut(s) 89, 106, 202, 450, 757
ApeKI GCWGC 1 cut(s) 310
ArsI GACNNNNNNTTYG 2 cut(s) 625, 657
AspS9I GGNCC 1 cut(s) 107
AsuC2I CCSGG 2 cut(s) 306, 307
AsuHPI GGTGA 3 cut(s) 74, 505, 584
AvaI CYCGRG 2 cut(s) 31, 305
BalI TGGCCA 1 cut(s) 759
BanI GGYRCC 1 cut(s) 566
Bbv12I GWGCWC 1 cut(s) 405
BbvI GCAGC 1 cut(s) 322
BccI CCATC 1 cut(s) 375
BciT130I CCWGG 1 cut(s) 105
BclI TGATCA 1 cut(s) 579
BcnI CCSGG 2 cut(s) 306, 307
BcoDI GTCTC 1 cut(s) 684
BisI GCNGC 2 cut(s) 145, 311
BlsI GCNGC 2 cut(s) 146, 312
Bme1390I CCNGG 3 cut(s) 105, 306, 307
BmeT110I CYCGRG 2 cut(s) 31, 305
BmgT120I GGNCC 1 cut(s) 107
BmiI GGNNCC 1 cut(s) 568
BmrFI CCNGG 3 cut(s) 105, 306, 307
BmrI ACTGGG 1 cut(s) 531
BmsI GCATC 2 cut(s) 513, 649
BmuI ACTGGG 1 cut(s) 531
BpuMI CCSGG 2 cut(s) 306, 307
BsaHI GRCGYC 2 cut(s) 11, 183
BsaJI CCNNGG 2 cut(s) 110, 305
Bsc4I CCNNNNNNNGG 4 cut(s) 280, 294, 522, 637
Bse118I RCCGGY 2 cut(s) 452, 569
Bse1I ACTGG 3 cut(s) 422, 526, 752
Bse3DI GCAATG 4 cut(s) 38, 340, 394, 435
BseBI CCWGG 1 cut(s) 105
BseDI CCNNGG 2 cut(s) 110, 305
BseGI GGATG 2 cut(s) 270, 386
BseLI CCNNNNNNNGG 4 cut(s) 280, 294, 522, 637
BseMI GCAATG 4 cut(s) 38, 340, 394, 435
BseMII CTCAG 1 cut(s) 432
BseNI ACTGG 3 cut(s) 422, 526, 752
BseRI GAGGAG 1 cut(s) 513
BseXI GCAGC 1 cut(s) 322
Bsh1236I CGCG 1 cut(s) 399
BshFI GGCC 5 cut(s) 91, 108, 204, 452, 759
BshNI GGYRCC 1 cut(s) 566
BsiHKAI GWGCWC 1 cut(s) 405
BsiHKCI CYCGRG 2 cut(s) 31, 305
BsiSI CCGG 3 cut(s) 306, 453, 570
BslI CCNNNNNNNGG 4 cut(s) 280, 294, 522, 637
BsmAI GTCTC 1 cut(s) 684
BsnI GGCC 5 cut(s) 91, 108, 204, 452, 759
BsoBI CYCGRG 2 cut(s) 31, 305
Bsp1286I GDGCHC 1 cut(s) 405
Bsp143I GATC 2 cut(s) 152, 579
BspACI CCGC 2 cut(s) 144, 475
BspANI GGCC 5 cut(s) 91, 108, 204, 452, 759
BspCNI CTCAG 1 cut(s) 433
BspFNI CGCG 1 cut(s) 399
BspLI GGNNCC 1 cut(s) 568
BspPI GGATC 1 cut(s) 160
BspT107I GGYRCC 1 cut(s) 566
BsrDI GCAATG 4 cut(s) 38, 340, 394, 435
BsrFI RCCGGY 2 cut(s) 452, 569
BsrI ACTGG 3 cut(s) 422, 526, 752
BssAI RCCGGY 2 cut(s) 452, 569
BssECI CCNNGG 2 cut(s) 110, 305
BssMI GATC 2 cut(s) 152, 579
BssNI GRCGYC 2 cut(s) 11, 183
BssT1I CCWWGG 1 cut(s) 110
Bst2UI CCWGG 1 cut(s) 105
Bst4CI ACNGT 1 cut(s) 214
Bst6I CTCTTC 1 cut(s) 324
BstACI GRCGYC 2 cut(s) 11, 183
BstC8I GCNNGC 6 cut(s) 120, 262, 302, 401, 454, 587
BstDEI CTNAG 2 cut(s) 441, 741
BstEII GGTNACC 1 cut(s) 173
BstF5I GGATG 2 cut(s) 270, 386
BstFNI CGCG 1 cut(s) 399
BstKTI GATC 2 cut(s) 155, 582
BstMAI GTCTC 1 cut(s) 684
BstMBI GATC 2 cut(s) 152, 579
BstMWI GCNNNNNNNGC 1 cut(s) 310
BstNI CCWGG 1 cut(s) 105
BstNSI RCATGY 2 cut(s) 122, 264
BstPI GGTNACC 1 cut(s) 173
BstSCI CCNGG 3 cut(s) 103, 304, 305
BstUI CGCG 1 cut(s) 399
BstV1I GCAGC 1 cut(s) 322
BsuRI GGCC 5 cut(s) 91, 108, 204, 452, 759
BtsCI GGATG 2 cut(s) 270, 386
BtsIMutI CAGTG 2 cut(s) 219, 429
Cac8I GCNNGC 6 cut(s) 120, 262, 302, 401, 454, 587
Cfr10I RCCGGY 2 cut(s) 452, 569
Cfr13I GGNCC 1 cut(s) 107
Cfr9I CCCGGG 1 cut(s) 305
CseI GACGC 1 cut(s) 723
Csp6I GTAC 2 cut(s) 59, 445
CviAII CATG 6 cut(s) 119, 125, 261, 323, 486, 726
CviQI GTAC 2 cut(s) 59, 445
DdeI CTNAG 2 cut(s) 441, 741
DpnI GATC 2 cut(s) 154, 581
DpnII GATC 2 cut(s) 152, 579
EaeI YGGCCR 1 cut(s) 757
Eam1104I CTCTTC 1 cut(s) 324
EarI CTCTTC 1 cut(s) 324
Eco130I CCWWGG 1 cut(s) 110
Eco57I CTGAAG 2 cut(s) 673, 766
Eco88I CYCGRG 2 cut(s) 31, 305
Eco91I GGTNACC 1 cut(s) 173
EcoO65I GGTNACC 1 cut(s) 173
EcoRII CCWGG 1 cut(s) 103
EcoT14I CCWWGG 1 cut(s) 110
ErhI CCWWGG 1 cut(s) 110
FaeI CATG 6 cut(s) 122, 128, 264, 326, 489, 729
FatI CATG 6 cut(s) 118, 124, 260, 322, 485, 725
FbaI TGATCA 1 cut(s) 579
Fnu4HI GCNGC 2 cut(s) 145, 311
FokI GGATG 2 cut(s) 257, 393
Fsp4HI GCNGC 2 cut(s) 145, 311
GluI GCNGC 2 cut(s) 145, 311
HaeIII GGCC 5 cut(s) 91, 108, 204, 452, 759
HapII CCGG 3 cut(s) 306, 453, 570
HgaI GACGC 1 cut(s) 723
Hin1I GRCGYC 2 cut(s) 11, 183
Hin1II CATG 6 cut(s) 122, 128, 264, 326, 489, 729
HinfI GANTC 5 cut(s) 284, 481, 510, 526, 677
HpaII CCGG 3 cut(s) 306, 453, 570
HphI GGTGA 3 cut(s) 74, 505, 584
Hpy166II GTNNAC 1 cut(s) 283
Hpy188I TCNGA 7 cut(s) 101, 366, 509, 645, 684, 692, 785
Hpy188III TCNNGA 2 cut(s) 150, 191
Hpy8I GTNNAC 1 cut(s) 283
Hpy99I CGWCG 3 cut(s) 185, 188, 605
HpyAV CCTTC 6 cut(s) 134, 188, 371, 589, 697, 764
HpyCH4III ACNGT 1 cut(s) 214
HpyCH4IV ACGT 2 cut(s) 183, 558
HpyCH4V TGCA 6 cut(s) 260, 300, 387, 392, 589, 662
HpyF10VI GCNNNNNNNGC 1 cut(s) 310
HpyF3I CTNAG 2 cut(s) 441, 741
HpySE526I ACGT 2 cut(s) 183, 558
Hsp92I GRCGYC 2 cut(s) 11, 183
Hsp92II CATG 6 cut(s) 122, 128, 264, 326, 489, 729
KroI GCCGGC 1 cut(s) 452
KroNI GCCGGC 1 cut(s) 454
Ksp22I TGATCA 1 cut(s) 579
Kzo9I GATC 2 cut(s) 152, 579
Lsp1109I GCAGC 1 cut(s) 322
LweI GCATC 2 cut(s) 513, 649
MaeII ACGT 2 cut(s) 183, 558
MaeIII GTNAC 3 cut(s) 173, 325, 368
MalI GATC 2 cut(s) 154, 581
MboI GATC 2 cut(s) 152, 579
MboII GAAGA 2 cut(s) 44, 311
MhlI GDGCHC 1 cut(s) 405
MlsI TGGCCA 1 cut(s) 759
MluCI AATT 1 cut(s) 752
MluNI TGGCCA 1 cut(s) 759
MlyI GAGTC 2 cut(s) 278, 686
MmeI TCCRAC 1 cut(s) 652
MnlI CCTC 8 cut(s) 38, 215, 371, 440, 491, 494, 557, 639
Mox20I TGGCCA 1 cut(s) 759
MroNI GCCGGC 1 cut(s) 452
MscI TGGCCA 1 cut(s) 759
MslI CAYNNNNRTG 2 cut(s) 123, 295
Msp20I TGGCCA 1 cut(s) 759
MspI CCGG 3 cut(s) 306, 453, 570
MspR9I CCNGG 3 cut(s) 105, 306, 307
MvaI CCWGG 1 cut(s) 105
MvnI CGCG 1 cut(s) 399
MwoI GCNNNNNNNGC 1 cut(s) 310
NaeI GCCGGC 1 cut(s) 454
NciI CCSGG 2 cut(s) 306, 307
NdeII GATC 2 cut(s) 152, 579
NgoMIV GCCGGC 1 cut(s) 452
NlaIII CATG 6 cut(s) 122, 128, 264, 326, 489, 729
NlaIV GGNNCC 1 cut(s) 568
NmuCI GTSAC 1 cut(s) 368
NspI RCATGY 2 cut(s) 122, 264
OliI CACNNNNGTG 1 cut(s) 295
PaeI GCATGC 2 cut(s) 122, 264
PdiI GCCGGC 1 cut(s) 454
PfeI GAWTC 3 cut(s) 481, 510, 526
PflMI CCANNNNNTGG 1 cut(s) 280
PkrI GCNGC 2 cut(s) 146, 312
PleI GAGTC 2 cut(s) 278, 685
PpsI GAGTC 2 cut(s) 278, 685
Psp6I CCWGG 1 cut(s) 103
PspEI GGTNACC 1 cut(s) 173
PspGI CCWGG 1 cut(s) 103
PspN4I GGNNCC 1 cut(s) 568
PspPI GGNCC 1 cut(s) 107
RsaI GTAC 2 cut(s) 60, 446
RsaNI GTAC 2 cut(s) 59, 445
RseI CAYNNNNRTG 2 cut(s) 123, 295
SatI GCNGC 2 cut(s) 145, 311
Sau3AI GATC 2 cut(s) 152, 579
Sau96I GGNCC 1 cut(s) 107
SchI GAGTC 2 cut(s) 278, 686
ScrFI CCNGG 3 cut(s) 105, 306, 307
SduI GDGCHC 1 cut(s) 405
SfaNI GCATC 2 cut(s) 513, 649
SmaI CCCGGG 1 cut(s) 307
SmiMI CAYNNNNRTG 2 cut(s) 123, 295
SphI GCATGC 2 cut(s) 122, 264
SrfI GCCCGGGC 1 cut(s) 307
Sse9I AATT 1 cut(s) 752
SsiI CCGC 2 cut(s) 144, 475
StyD4I CCNGG 3 cut(s) 103, 304, 305
StyI CCWWGG 1 cut(s) 110
TaaI ACNGT 1 cut(s) 214
TaiI ACGT 2 cut(s) 186, 561
TaqI TCGA 4 cut(s) 5, 207, 408, 719
TasI AATT 1 cut(s) 752
TatI WGTACW 1 cut(s) 444
TauI GCSGC 1 cut(s) 147
TfiI GAWTC 3 cut(s) 481, 510, 526
TscAI CASTG 2 cut(s) 219, 429
TseFI GTSAC 1 cut(s) 368
TseI GCWGC 1 cut(s) 310
Tsp45I GTSAC 1 cut(s) 368
TspDTI ATGAA 4 cut(s) 27, 311, 641, 714
TspMI CCCGGG 1 cut(s) 305
TspRI CASTG 2 cut(s) 219, 429
Van91I CCANNNNNTGG 1 cut(s) 280
XceI RCATGY 2 cut(s) 122, 264
XmaI CCCGGG 1 cut(s) 305
ZraI GACGTC 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.