MD12G1105900.v1.1

expansin-A9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0004238
Physical Location & Seq
Forward (+)
16762207 .. 16765004
2798 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1105900.v1.1.491

Sequence Viewer

Length: 792 bp
ATGACATTACCTACTTTGCAAGCTCTTTTGGGTTCCTTTTCTGTCTTTCTTTTTGTGCTAGTTGACCATGTCCATGTGGTTGCAGCCAACCGCGGCCGCTTTGGAGTTCACCACCCCATAAAGCACCATAAGCCAAGGTTCACTGCTGGCCCTTGGAAGCAAGCTCATGCCACATTCTACGAGGGCGGCTCAGGAACATTTGGTGGAGCTTGTGGTTACCATTCAATGCTTTGTTCAACAATGGACTGTCATGTGGTGCATGCTAGCTATGAAATCAAGTGTGTGGATGAGCCCCAGTGGTGTAAGCCAGGGAAGCCTATTCTCACTGTTACTGCAACCAACAATTGCCCACCAAATTGGAATCAGGCTAGTGACAATGGAGGATGGTGCAATCCGCCACGCGAGCATTTTGATATAGCCAAGCCCGTGTTCCTCAACATTGCTGAGTACAAGGCGGGCATTATTCCAGTCACGTACCGCAGGGTTCCATGCCAGAAGAAAGGAGGCATGCGATTCACAATAACCGGAAACCCTTATTTCAATGAAGTGTTGGTATGGAACGTGGGAGGAGCTGGGGACATCATCAGCGTGCAGGTGAAGGGCTCCGACAAGCTCAAATGGACAACAATGAGTCGGATGTGGGGTCAGCGGTGGGTTACAGGTGCTAAGATGGTCGGTGAGTCACTGACCTTCCGAGTTGAAGCAAGTGATAGAAGACACTCAACTTCATGGCATGTTGCCCCCAAGACTTGGCAGTTTGGCCAGACTTTTGAAGGGAAGAACTTCCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

264

Amino Acids

29.43

Weight (kDa)

9.47

Isoelectric Point (pI)

26.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_1 PF03330 88 - 159 1e-10 Lytic transglycolase
Expansin_C PF01357 170 - 248 2.2e-23 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 583
Acc36I ACCTGC 1 cut(s) 583
AccB7I CCANNNNNTGG 1 cut(s) 750
AccII CGCG 2 cut(s) 93, 402
AciI CCGC 8 cut(s) 91, 93, 97, 186, 395, 455, 478, 649
AcoI YGGCCR 2 cut(s) 94, 760
AfaI GTAC 2 cut(s) 449, 476
AfiI CCNNNNNNNGG 1 cut(s) 750
AgsI TTSAA 5 cut(s) 225, 237, 541, 701, 773
AjnI CCWGG 1 cut(s) 307
AjuI GAANNNNNNNTTGG 2 cut(s) 413, 445
AluBI AGCT 6 cut(s) 23, 164, 209, 267, 572, 613
AluI AGCT 6 cut(s) 23, 164, 209, 267, 572, 613
AoxI GGCC 3 cut(s) 94, 148, 760
ApeKI GCWGC 1 cut(s) 83
Asp700I GAANNNNTTC 1 cut(s) 782
AspS9I GGNCC 1 cut(s) 149
AsuHPI GGTGA 3 cut(s) 101, 607, 689
AsuNHI GCTAGC 1 cut(s) 263
BalI TGGCCA 1 cut(s) 762
BanII GRGCYC 2 cut(s) 294, 605
BbsI GAAGAC 1 cut(s) 721
BbvI GCAGC 1 cut(s) 95
BccI CCATC 2 cut(s) 378, 664
BciT130I CCWGG 1 cut(s) 309
BfaI CTAG 3 cut(s) 59, 264, 369
BfuAI ACCTGC 1 cut(s) 583
BisI GCNGC 4 cut(s) 84, 94, 97, 187
BlsI GCNGC 4 cut(s) 85, 95, 98, 188
Bme1390I CCNGG 1 cut(s) 309
BmgT120I GGNCC 1 cut(s) 149
BmiI GGNNCC 3 cut(s) 34, 486, 604
BmrFI CCNGG 1 cut(s) 309
BmrI ACTGGG 1 cut(s) 289
BmtI GCTAGC 1 cut(s) 267
BmuI ACTGGG 1 cut(s) 289
BpiI GAAGAC 1 cut(s) 721
BplI GAGNNNNNCTC 2 cut(s) 173, 205
Bpu10I CCTNAGC 1 cut(s) 190
BsaAI YACGTR 1 cut(s) 474
BsaJI CCNNGG 4 cut(s) 91, 134, 152, 308
BsaWI WCCGGW 1 cut(s) 524
Bsc4I CCNNNNNNNGG 1 cut(s) 750
Bse1I ACTGG 2 cut(s) 295, 467
Bse3DI GCAATG 1 cut(s) 438
BseBI CCWGG 1 cut(s) 309
BseDI CCNNGG 4 cut(s) 91, 134, 152, 308
BseGI GGATG 3 cut(s) 292, 389, 642
BseLI CCNNNNNNNGG 1 cut(s) 750
BseMI GCAATG 1 cut(s) 438
BseMII CTCAG 2 cut(s) 204, 435
BseNI ACTGG 2 cut(s) 295, 467
BseRI GAGGAG 1 cut(s) 582
BseX3I CGGCCG 1 cut(s) 94
BseXI GCAGC 1 cut(s) 95
BseYI CCCAGC 1 cut(s) 572
BsgI GTGCAG 1 cut(s) 611
Bsh1236I CGCG 2 cut(s) 93, 402
Bsh1285I CGRYCG 1 cut(s) 97
BshFI GGCC 3 cut(s) 96, 150, 762
BsiEI CGRYCG 1 cut(s) 97
BsiSI CCGG 1 cut(s) 525
BslFI GGGAC 1 cut(s) 590
BslI CCNNNNNNNGG 1 cut(s) 750
BsmFI GGGAC 1 cut(s) 590
BsnI GGCC 3 cut(s) 96, 150, 762
Bsp1286I GDGCHC 2 cut(s) 294, 605
BspACI CCGC 8 cut(s) 91, 93, 97, 186, 395, 455, 478, 649
BspANI GGCC 3 cut(s) 96, 150, 762
BspCNI CTCAG 2 cut(s) 203, 436
BspFNI CGCG 2 cut(s) 93, 402
BspLI GGNNCC 3 cut(s) 34, 486, 604
BspMI ACCTGC 1 cut(s) 583
BspOI GCTAGC 1 cut(s) 267
BsrDI GCAATG 1 cut(s) 438
BsrI ACTGG 2 cut(s) 295, 467
BssECI CCNNGG 4 cut(s) 91, 134, 152, 308
BssT1I CCWWGG 2 cut(s) 134, 152
Bst2UI CCWGG 1 cut(s) 309
Bst4CI ACNGT 2 cut(s) 248, 328
BstBAI YACGTR 1 cut(s) 474
BstC8I GCNNGC 9 cut(s) 21, 148, 162, 261, 265, 404, 457, 509, 590
BstDEI CTNAG 3 cut(s) 190, 444, 666
BstDSI CCRYGG 1 cut(s) 91
BstEII GGTNACC 1 cut(s) 215
BstF5I GGATG 3 cut(s) 292, 389, 642
BstFNI CGCG 2 cut(s) 93, 402
BstMCI CGRYCG 1 cut(s) 97
BstMWI GCNNNNNNNGC 3 cut(s) 130, 313, 403
BstNI CCWGG 1 cut(s) 309
BstNSI RCATGY 3 cut(s) 263, 511, 737
BstPI GGTNACC 1 cut(s) 215
BstSCI CCNGG 1 cut(s) 307
BstUI CGCG 2 cut(s) 93, 402
BstV1I GCAGC 1 cut(s) 95
BstV2I GAAGAC 1 cut(s) 721
BstXI CCANNNNNNTGG 1 cut(s) 357
BstZI CGGCCG 1 cut(s) 94
BsuRI GGCC 3 cut(s) 96, 150, 762
BtgI CCRYGG 1 cut(s) 91
BtsCI GGATG 3 cut(s) 292, 389, 642
BtsI GCAGTG 1 cut(s) 141
BtsIMutI CAGTG 4 cut(s) 141, 302, 324, 683
BveI ACCTGC 1 cut(s) 583
Cac8I GCNNGC 9 cut(s) 21, 148, 162, 261, 265, 404, 457, 509, 590
CciNI GCGGCCGC 1 cut(s) 94
Cfr13I GGNCC 1 cut(s) 149
Cfr42I CCGCGG 1 cut(s) 94
Csp6I GTAC 2 cut(s) 448, 475
CviAII CATG 9 cut(s) 68, 74, 167, 251, 260, 489, 508, 729, 734
CviQI GTAC 2 cut(s) 448, 475
DdeI CTNAG 3 cut(s) 190, 444, 666
EaeI YGGCCR 2 cut(s) 94, 760
EagI CGGCCG 1 cut(s) 94
EciI GGCGGA 1 cut(s) 384
EclXI CGGCCG 1 cut(s) 94
Eco130I CCWWGG 2 cut(s) 134, 152
Eco24I GRGCYC 2 cut(s) 294, 605
Eco52I CGGCCG 1 cut(s) 94
Eco91I GGTNACC 1 cut(s) 215
EcoO65I GGTNACC 1 cut(s) 215
EcoRII CCWGG 1 cut(s) 307
EcoT14I CCWWGG 2 cut(s) 134, 152
EcoT38I GRGCYC 2 cut(s) 294, 605
ErhI CCWWGG 2 cut(s) 134, 152
FaeI CATG 9 cut(s) 71, 77, 170, 254, 263, 492, 511, 732, 737
FaqI GGGAC 1 cut(s) 590
FatI CATG 9 cut(s) 67, 73, 166, 250, 259, 488, 507, 728, 733
FauI CCCGC 1 cut(s) 448
Fnu4HI GCNGC 4 cut(s) 84, 94, 97, 187
FokI GGATG 3 cut(s) 299, 396, 649
FriOI GRGCYC 2 cut(s) 294, 605
Fsp4HI GCNGC 4 cut(s) 84, 94, 97, 187
FspBI CTAG 3 cut(s) 59, 264, 369
GluI GCNGC 4 cut(s) 84, 94, 97, 187
GsaI CCCAGC 1 cut(s) 576
HaeIII GGCC 3 cut(s) 96, 150, 762
HapII CCGG 1 cut(s) 525
Hin1II CATG 9 cut(s) 71, 77, 170, 254, 263, 492, 511, 732, 737
HincII GTYRAC 1 cut(s) 64
HindII GTYRAC 1 cut(s) 64
HinfI GANTC 4 cut(s) 361, 513, 631, 680
HpaII CCGG 1 cut(s) 525
HphI GGTGA 3 cut(s) 101, 607, 689
Hpy166II GTNNAC 3 cut(s) 64, 109, 141
Hpy188I TCNGA 4 cut(s) 607, 636, 695, 788
Hpy188III TCNNGA 1 cut(s) 192
Hpy8I GTNNAC 3 cut(s) 64, 109, 141
HpyAV CCTTC 3 cut(s) 592, 700, 767
HpyCH4III ACNGT 2 cut(s) 248, 328
HpyCH4IV ACGT 2 cut(s) 473, 561
HpyCH4V TGCA 6 cut(s) 19, 83, 259, 335, 390, 592
HpyF10VI GCNNNNNNNGC 3 cut(s) 130, 313, 403
HpyF3I CTNAG 3 cut(s) 190, 444, 666
HpySE526I ACGT 2 cut(s) 473, 561
Hsp92II CATG 9 cut(s) 71, 77, 170, 254, 263, 492, 511, 732, 737
KspI CCGCGG 1 cut(s) 94
LmnI GCTCC 3 cut(s) 206, 569, 608
Lsp1109I GCAGC 1 cut(s) 95
MaeI CTAG 3 cut(s) 59, 264, 369
MaeII ACGT 2 cut(s) 473, 561
MaeIII GTNAC 6 cut(s) 215, 328, 371, 469, 655, 681
MboII GAAGA 3 cut(s) 508, 726, 790
MfeI CAATTG 1 cut(s) 343
MhlI GDGCHC 2 cut(s) 294, 605
MlsI TGGCCA 1 cut(s) 762
MluCI AATT 2 cut(s) 343, 355
MluNI TGGCCA 1 cut(s) 762
MlyI GAGTC 2 cut(s) 640, 689
MmeI TCCRAC 2 cut(s) 614, 630
MnlI CCTC 5 cut(s) 175, 374, 443, 497, 560
Mox20I TGGCCA 1 cut(s) 762
MroXI GAANNNNTTC 1 cut(s) 782
MscI TGGCCA 1 cut(s) 762
MslI CAYNNNNRTG 2 cut(s) 72, 587
Msp20I TGGCCA 1 cut(s) 762
MspA1I CMGCKG 2 cut(s) 93, 649
MspI CCGG 1 cut(s) 525
MspR9I CCNGG 1 cut(s) 309
MunI CAATTG 1 cut(s) 343
MvaI CCWGG 1 cut(s) 309
MvnI CGCG 2 cut(s) 93, 402
MwoI GCNNNNNNNGC 3 cut(s) 130, 313, 403
NheI GCTAGC 1 cut(s) 263
NlaIII CATG 9 cut(s) 71, 77, 170, 254, 263, 492, 511, 732, 737
NlaIV GGNNCC 3 cut(s) 34, 486, 604
NmuCI GTSAC 3 cut(s) 371, 469, 681
NotI GCGGCCGC 1 cut(s) 94
NspI RCATGY 3 cut(s) 263, 511, 737
PaeI GCATGC 2 cut(s) 263, 511
PaqCI CACCTGC 1 cut(s) 583
PdmI GAANNNNTTC 1 cut(s) 782
PfeI GAWTC 2 cut(s) 361, 513
PflFI GACNNNGTC 1 cut(s) 68
PflMI CCANNNNNTGG 1 cut(s) 750
PkrI GCNGC 4 cut(s) 85, 95, 98, 188
PleI GAGTC 2 cut(s) 639, 688
PpsI GAGTC 2 cut(s) 639, 688
Ppu21I YACGTR 1 cut(s) 474
Psp6I CCWGG 1 cut(s) 307
PspEI GGTNACC 1 cut(s) 215
PspFI CCCAGC 1 cut(s) 572
PspGI CCWGG 1 cut(s) 307
PspN4I GGNNCC 3 cut(s) 34, 486, 604
PspPI GGNCC 1 cut(s) 149
PsyI GACNNNGTC 1 cut(s) 68
RsaI GTAC 2 cut(s) 449, 476
RsaNI GTAC 2 cut(s) 448, 475
RseI CAYNNNNRTG 2 cut(s) 72, 587
SacII CCGCGG 1 cut(s) 94
SatI GCNGC 4 cut(s) 84, 94, 97, 187
Sau96I GGNCC 1 cut(s) 149
SchI GAGTC 2 cut(s) 640, 689
ScrFI CCNGG 1 cut(s) 309
SduI GDGCHC 2 cut(s) 294, 605
Sfr303I CCGCGG 1 cut(s) 94
SgrBI CCGCGG 1 cut(s) 94
SmiMI CAYNNNNRTG 2 cut(s) 72, 587
SphI GCATGC 2 cut(s) 263, 511
Sse9I AATT 2 cut(s) 343, 355
SsiI CCGC 8 cut(s) 91, 93, 97, 186, 395, 455, 478, 649
SspMI CTAG 3 cut(s) 59, 264, 369
StyD4I CCNGG 1 cut(s) 307
StyI CCWWGG 2 cut(s) 134, 152
TaaI ACNGT 2 cut(s) 248, 328
TaiI ACGT 2 cut(s) 476, 564
TasI AATT 2 cut(s) 343, 355
TatI WGTACW 1 cut(s) 447
TauI GCSGC 3 cut(s) 96, 99, 189
TfiI GAWTC 2 cut(s) 361, 513
TscAI CASTG 4 cut(s) 148, 302, 331, 690
TseFI GTSAC 3 cut(s) 371, 469, 681
TseI GCWGC 1 cut(s) 83
Tsp45I GTSAC 3 cut(s) 371, 469, 681
TspDTI ATGAA 3 cut(s) 285, 558, 717
TspRI CASTG 4 cut(s) 148, 302, 331, 690
Tth111I GACNNNGTC 1 cut(s) 68
Van91I CCANNNNNTGG 1 cut(s) 750
XceI RCATGY 3 cut(s) 263, 511, 737
XmnI GAANNNNTTC 1 cut(s) 782
XspI CTAG 3 cut(s) 59, 264, 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.