Rh3AG163700

expansin-A9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0004238
Physical Location & Seq
Reverse (-)
15103278 .. 15104551
1274 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG163700.1

Sequence Viewer

Length: 780 bp
ATGTCAATGAGTTCTGCCCTCCTGGGTTCATTGCTTTTCATTCTTTTCGTGTTACCTGACCATGTCACCGCTCGCCATAAGCCAAAGTTCAGACCTGGCCCTTGGAAGCATGCTCATGCCACATTCTACGAAGGCGGCTCTGGATCATTTGGTGGTGCTTGTGGTTACCACGACGTCGTTCAAGAAGGCTATGGCCTCGACACGGTGGCTTTGAGCGACACCTTGTTCAACAATGGGCAGTCGTGTGGTGCATGTTACGATATCCAATGTGTGGACTCCCCACAATGGTGCAAGCCCGGGCAGCCAACTCTTCATGTTACAGCAACCAACCATTGCCCCCCGAATTATAACCTTCCCAGTGACAATGGAGGATGGTGCAATGCACCACGTGAGCACTTCGATATAGCCAAGCCGGTGTTCCTCAACATTGCAGAGTACAAGGCTGGCATAGTACCAATCATATACCGCAGGGTTCAGTGCCAAAAGAGAGGAGGCATCCGATTCACGATAACCGGGAATCCTTACTTCAATGAAGTGTTGGTGTGGAACGTGGGAGGTGCCGGAGATGTCATAAGCGTGCAAGTGAAGGGCCATGACAAGCTCAAATGGACAACGATGAAGCGAATGTGGGGTCAGAGGTGGATTACTGATGCCAAGTTGGTTGGGGAGTCTCTGACCTTCAGAGTAGAAGCAAGTGATAAAAGACGCTCAACTTCTTGGCATATAGCCCCTCAGAACTGGCAGTTTGGCCAGACCTTTGAAGGCAAGAACTTTCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

259

Amino Acids

28.95

Weight (kDa)

9.0

Isoelectric Point (pI)

41.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXPB1_D1 PF22514 40 - 161 3.5e-06 EXPB1-like domain 1
DPBB_1 PF03330 68 - 155 9.5e-18 Lytic transglycolase
Expansin_C PF01357 166 - 244 6.1e-24 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 348
AatII GACGTC 1 cut(s) 177
AccB1I GGYRCC 1 cut(s) 557
AccB7I CCANNNNNTGG 1 cut(s) 271
AccBSI CCGCTC 1 cut(s) 71
AciI CCGC 3 cut(s) 69, 135, 466
AclWI GGATC 1 cut(s) 151
AcoI YGGCCR 1 cut(s) 748
AcuI CTGAAG 1 cut(s) 664
AcvI CACGTG 1 cut(s) 389
AcyI GRCGYC 1 cut(s) 174
AdeI CACNNNGTG 1 cut(s) 389
AfaI GTAC 2 cut(s) 437, 453
AfiI CCNNNNNNNGG 3 cut(s) 202, 271, 285
AgsI TTSAA 4 cut(s) 182, 229, 529, 761
AjnI CCWGG 2 cut(s) 21, 94
AjuI GAANNNNNNNTTGG 2 cut(s) 401, 433
AleI CACNNNNGTG 1 cut(s) 286
AluBI AGCT 1 cut(s) 601
AluI AGCT 1 cut(s) 601
Alw21I GWGCWC 1 cut(s) 396
Alw26I GTCTC 1 cut(s) 675
AlwI GGATC 1 cut(s) 151
Ama87I CYCGRG 1 cut(s) 296
AoxI GGCC 4 cut(s) 97, 193, 589, 748
ApeKI GCWGC 1 cut(s) 301
ArsI GACNNNNNNTTYG 2 cut(s) 616, 648
AspS9I GGNCC 2 cut(s) 98, 589
AsuC2I CCSGG 3 cut(s) 297, 298, 514
AsuHPI GGTGA 1 cut(s) 58
AvaI CYCGRG 1 cut(s) 296
BalI TGGCCA 1 cut(s) 750
BanI GGYRCC 1 cut(s) 557
BbrPI CACGTG 1 cut(s) 389
Bbv12I GWGCWC 1 cut(s) 396
BbvI GCAGC 1 cut(s) 313
BccI CCATC 1 cut(s) 366
BciT130I CCWGG 2 cut(s) 23, 96
BcnI CCSGG 3 cut(s) 297, 298, 514
BcoDI GTCTC 1 cut(s) 675
BisI GCNGC 2 cut(s) 136, 302
BlsI GCNGC 2 cut(s) 137, 303
Bme1390I CCNGG 5 cut(s) 23, 96, 297, 298, 514
BmeT110I CYCGRG 1 cut(s) 296
BmgT120I GGNCC 2 cut(s) 98, 589
BmiI GGNNCC 1 cut(s) 559
BmrFI CCNGG 5 cut(s) 23, 96, 297, 298, 514
BmrI ACTGGG 1 cut(s) 351
BmsI GCATC 2 cut(s) 504, 640
BmuI ACTGGG 1 cut(s) 351
BpuMI CCSGG 3 cut(s) 297, 298, 514
BsaAI YACGTR 1 cut(s) 389
BsaHI GRCGYC 1 cut(s) 174
BsaJI CCNNGG 3 cut(s) 22, 101, 296
Bsc4I CCNNNNNNNGG 3 cut(s) 202, 271, 285
Bse118I RCCGGY 1 cut(s) 412
Bse1I ACTGG 2 cut(s) 357, 743
Bse3DI GCAATG 4 cut(s) 29, 331, 385, 426
BseBI CCWGG 2 cut(s) 23, 96
BseDI CCNNGG 3 cut(s) 22, 101, 296
BseGI GGATG 2 cut(s) 377, 495
BseLI CCNNNNNNNGG 3 cut(s) 202, 271, 285
BseMI GCAATG 4 cut(s) 29, 331, 385, 426
BseMII CTCAG 1 cut(s) 746
BseNI ACTGG 2 cut(s) 357, 743
BseRI GAGGAG 1 cut(s) 504
BseXI GCAGC 1 cut(s) 313
BshFI GGCC 4 cut(s) 99, 195, 591, 750
BshNI GGYRCC 1 cut(s) 557
BsiHKAI GWGCWC 1 cut(s) 396
BsiHKCI CYCGRG 1 cut(s) 296
BsiSI CCGG 4 cut(s) 297, 413, 513, 561
BslI CCNNNNNNNGG 3 cut(s) 202, 271, 285
BsmAI GTCTC 1 cut(s) 675
BsnI GGCC 4 cut(s) 99, 195, 591, 750
BsoBI CYCGRG 1 cut(s) 296
Bsp1286I GDGCHC 1 cut(s) 396
Bsp143I GATC 1 cut(s) 143
BspACI CCGC 3 cut(s) 69, 135, 466
BspANI GGCC 4 cut(s) 99, 195, 591, 750
BspCNI CTCAG 1 cut(s) 745
BspLI GGNNCC 1 cut(s) 559
BspPI GGATC 1 cut(s) 151
BspT107I GGYRCC 1 cut(s) 557
BsrBI CCGCTC 1 cut(s) 71
BsrDI GCAATG 4 cut(s) 29, 331, 385, 426
BsrFI RCCGGY 1 cut(s) 412
BsrI ACTGG 2 cut(s) 357, 743
BssAI RCCGGY 1 cut(s) 412
BssECI CCNNGG 3 cut(s) 22, 101, 296
BssMI GATC 1 cut(s) 143
BssNI GRCGYC 1 cut(s) 174
BssT1I CCWWGG 1 cut(s) 101
Bst2UI CCWGG 2 cut(s) 23, 96
Bst4CI ACNGT 1 cut(s) 205
Bst6I CTCTTC 1 cut(s) 315
BstACI GRCGYC 1 cut(s) 174
BstBAI YACGTR 1 cut(s) 389
BstC8I GCNNGC 5 cut(s) 73, 111, 293, 445, 578
BstDEI CTNAG 1 cut(s) 732
BstEII GGTNACC 1 cut(s) 164
BstF5I GGATG 2 cut(s) 377, 495
BstKTI GATC 1 cut(s) 146
BstMAI GTCTC 1 cut(s) 675
BstMBI GATC 1 cut(s) 143
BstMWI GCNNNNNNNGC 1 cut(s) 301
BstNI CCWGG 2 cut(s) 23, 96
BstNSI RCATGY 2 cut(s) 113, 255
BstPI GGTNACC 1 cut(s) 164
BstSCI CCNGG 5 cut(s) 21, 94, 295, 296, 512
BstV1I GCAGC 1 cut(s) 313
BsuRI GGCC 4 cut(s) 99, 195, 591, 750
BtsCI GGATG 2 cut(s) 377, 495
BtsIMutI CAGTG 2 cut(s) 364, 482
Cac8I GCNNGC 5 cut(s) 73, 111, 293, 445, 578
Cfr10I RCCGGY 1 cut(s) 412
Cfr13I GGNCC 2 cut(s) 98, 589
Cfr9I CCCGGG 1 cut(s) 296
CseI GACGC 1 cut(s) 714
Csp6I GTAC 2 cut(s) 436, 452
CviAII CATG 6 cut(s) 62, 110, 116, 252, 314, 593
CviQI GTAC 2 cut(s) 436, 452
DdeI CTNAG 1 cut(s) 732
DpnI GATC 1 cut(s) 145
DpnII GATC 1 cut(s) 143
DraIII CACNNNGTG 1 cut(s) 389
EaeI YGGCCR 1 cut(s) 748
Eam1104I CTCTTC 1 cut(s) 315
EarI CTCTTC 1 cut(s) 315
Eco130I CCWWGG 1 cut(s) 101
Eco32I GATATC 1 cut(s) 262
Eco57I CTGAAG 1 cut(s) 664
Eco72I CACGTG 1 cut(s) 389
Eco88I CYCGRG 1 cut(s) 296
Eco91I GGTNACC 1 cut(s) 164
EcoO65I GGTNACC 1 cut(s) 164
EcoRII CCWGG 2 cut(s) 21, 94
EcoRV GATATC 1 cut(s) 262
EcoT14I CCWWGG 1 cut(s) 101
ErhI CCWWGG 1 cut(s) 101
FaeI CATG 6 cut(s) 65, 113, 119, 255, 317, 596
FatI CATG 6 cut(s) 61, 109, 115, 251, 313, 592
Fnu4HI GCNGC 2 cut(s) 136, 302
FokI GGATG 2 cut(s) 384, 482
Fsp4HI GCNGC 2 cut(s) 136, 302
GluI GCNGC 2 cut(s) 136, 302
HaeIII GGCC 4 cut(s) 99, 195, 591, 750
HapII CCGG 4 cut(s) 297, 413, 513, 561
HgaI GACGC 1 cut(s) 714
Hin1I GRCGYC 1 cut(s) 174
Hin1II CATG 6 cut(s) 65, 113, 119, 255, 317, 596
HinfI GANTC 4 cut(s) 275, 501, 517, 668
HpaII CCGG 4 cut(s) 297, 413, 513, 561
HphI GGTGA 1 cut(s) 58
Hpy166II GTNNAC 1 cut(s) 274
Hpy188I TCNGA 6 cut(s) 92, 500, 636, 675, 683, 735
Hpy188III TCNNGA 3 cut(s) 141, 182, 505
Hpy8I GTNNAC 1 cut(s) 274
Hpy99I CGWCG 2 cut(s) 176, 179
HpyAV CCTTC 6 cut(s) 125, 179, 362, 580, 688, 755
HpyCH4III ACNGT 1 cut(s) 205
HpyCH4IV ACGT 3 cut(s) 174, 388, 549
HpyCH4V TGCA 6 cut(s) 251, 291, 378, 383, 431, 580
HpyF10VI GCNNNNNNNGC 1 cut(s) 301
HpyF3I CTNAG 1 cut(s) 732
HpySE526I ACGT 3 cut(s) 174, 388, 549
Hsp92I GRCGYC 1 cut(s) 174
Hsp92II CATG 6 cut(s) 65, 113, 119, 255, 317, 596
Kzo9I GATC 1 cut(s) 143
Lsp1109I GCAGC 1 cut(s) 313
LweI GCATC 2 cut(s) 504, 640
MaeII ACGT 3 cut(s) 174, 388, 549
MaeIII GTNAC 6 cut(s) 51, 64, 164, 254, 316, 359
MalI GATC 1 cut(s) 145
MbiI CCGCTC 1 cut(s) 71
MboI GATC 1 cut(s) 143
MboII GAAGA 1 cut(s) 302
MhlI GDGCHC 1 cut(s) 396
MlsI TGGCCA 1 cut(s) 750
MluCI AATT 1 cut(s) 343
MluNI TGGCCA 1 cut(s) 750
MlyI GAGTC 2 cut(s) 269, 677
MnlI CCTC 9 cut(s) 29, 206, 362, 431, 482, 485, 548, 630, 741
Mox20I TGGCCA 1 cut(s) 750
MscI TGGCCA 1 cut(s) 750
MslI CAYNNNNRTG 3 cut(s) 114, 286, 575
Msp20I TGGCCA 1 cut(s) 750
MspI CCGG 4 cut(s) 297, 413, 513, 561
MspR9I CCNGG 5 cut(s) 23, 96, 297, 298, 514
MvaI CCWGG 2 cut(s) 23, 96
MwoI GCNNNNNNNGC 1 cut(s) 301
NciI CCSGG 3 cut(s) 297, 298, 514
NdeII GATC 1 cut(s) 143
NlaIII CATG 6 cut(s) 65, 113, 119, 255, 317, 596
NlaIV GGNNCC 1 cut(s) 559
NmuCI GTSAC 2 cut(s) 64, 359
NspI RCATGY 2 cut(s) 113, 255
OliI CACNNNNGTG 1 cut(s) 286
PaeI GCATGC 1 cut(s) 113
PfeI GAWTC 2 cut(s) 501, 517
PflFI GACNNNGTC 1 cut(s) 62
PflMI CCANNNNNTGG 1 cut(s) 271
PkrI GCNGC 2 cut(s) 137, 303
PleI GAGTC 2 cut(s) 269, 676
PmaCI CACGTG 1 cut(s) 389
PmlI CACGTG 1 cut(s) 389
PpsI GAGTC 2 cut(s) 269, 676
Ppu21I YACGTR 1 cut(s) 389
PsiI TTATAA 1 cut(s) 348
Psp6I CCWGG 2 cut(s) 21, 94
PspCI CACGTG 1 cut(s) 389
PspEI GGTNACC 1 cut(s) 164
PspGI CCWGG 2 cut(s) 21, 94
PspN4I GGNNCC 1 cut(s) 559
PspPI GGNCC 2 cut(s) 98, 589
PsyI GACNNNGTC 1 cut(s) 62
RsaI GTAC 2 cut(s) 437, 453
RsaNI GTAC 2 cut(s) 436, 452
RseI CAYNNNNRTG 3 cut(s) 114, 286, 575
SatI GCNGC 2 cut(s) 136, 302
Sau3AI GATC 1 cut(s) 143
Sau96I GGNCC 2 cut(s) 98, 589
SchI GAGTC 2 cut(s) 269, 677
ScrFI CCNGG 5 cut(s) 23, 96, 297, 298, 514
SduI GDGCHC 1 cut(s) 396
SfaNI GCATC 2 cut(s) 504, 640
SmaI CCCGGG 1 cut(s) 298
SmiMI CAYNNNNRTG 3 cut(s) 114, 286, 575
SphI GCATGC 1 cut(s) 113
SrfI GCCCGGGC 1 cut(s) 298
Sse9I AATT 1 cut(s) 343
SsiI CCGC 3 cut(s) 69, 135, 466
StyD4I CCNGG 5 cut(s) 21, 94, 295, 296, 512
StyI CCWWGG 1 cut(s) 101
TaaI ACNGT 1 cut(s) 205
TaiI ACGT 3 cut(s) 177, 391, 552
TaqI TCGA 3 cut(s) 198, 399, 775
TasI AATT 1 cut(s) 343
TatI WGTACW 1 cut(s) 435
TauI GCSGC 1 cut(s) 138
TfiI GAWTC 2 cut(s) 501, 517
TscAI CASTG 2 cut(s) 364, 482
TseFI GTSAC 2 cut(s) 64, 359
TseI GCWGC 1 cut(s) 301
Tsp45I GTSAC 2 cut(s) 64, 359
TspDTI ATGAA 5 cut(s) 18, 28, 302, 546, 632
TspMI CCCGGG 1 cut(s) 296
TspRI CASTG 2 cut(s) 364, 482
Tth111I GACNNNGTC 1 cut(s) 62
Van91I CCANNNNNTGG 1 cut(s) 271
XceI RCATGY 2 cut(s) 113, 255
XmaI CCCGGG 1 cut(s) 296
ZraI GACGTC 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.