pycom12g11520

expansin-A9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0004238
Physical Location & Seq
Forward (+)
13741898 .. 13743147
1250 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g11520.1

Sequence Viewer

Length: 1035 bp
ATGACATTGCCTAATCTGCGAGCTCTTTTGGGTTCCTTGGCTGTCTTTCTTTTTGTGCTAGCTGACCATCTCCATGTGGTTGCCGCCAACCGCGGCCGGTTTGGAGTTCACCACCCCATAAAGCACCATAAGCCAAGGTTCACTGCTGGCCCTTGGAAGCAAGCTCATGCCACATTCTACGAAGGCGGCTCGGGAACGTTTGGCGGAGCTTGTGGTTACCACGACGTGGTTCAAGAAGGCTATGGCCTCGAGACAGTGGCTTTGAGCAATGCTTTGTTCAACAATGGGCTGTCATGTGGTGCATGCTATGAAATGAAGTGTGTGGATGAGCCGCAATGGTGCAAGCCAGGGAACCCTATTCTCACTGTTACTGCAACCAACAATTGCCCACCAAATTGGAATCAGGCTAGTGACAATGGAGGATGGTGCAATCCGCCACGCGAGCATTTCGATATAGCCAAGCCTGTGTTCCTCAACATTGCTGAGTACAAGGCGGGCATTATTCCTGTCGCGTACCGCAGGGTTCCATGTCAGAAGAAAGGGGGCATGCGATTCACAATAACCGGGAACCCTTATTTCAATGAAGTGTTGGTATGGAATGTGGGAGGAGCTGGGGACATCATCAGCGTGCAGGTGAAGGGCTCCGACAAGCTCAAATGGACAGCAATGAGTCAGATGTGGGGTCAGCGGTGGGTTACCGGTGCCAAGATGGTCGGTGAGTCACTGACCTTCCGAGTTGAAGCAAGTGATAAACGACACTCAACTTCATGGCATGTTGCGCCCAAGACTTGGCAGTTTGGCCAGACTTTTGAAGGCAAGAACGGCCAAGATCGATCAAAAGCACATCATCAGCTTCAATGCTTCGTGCATGTTGAAATCCTCCACACCATATTATTTGAACTAGTTTGGCTTGTCATTACCAATGTATACAAACTAACCATGGAATTCTTGCCCGACCATGTAATAATAAAAACAAGGCACTCATATACACCTTACCACTTTTTCGTAAATTTCCTTCTTCAATATTTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

345

Amino Acids

38.71

Weight (kDa)

8.98

Isoelectric Point (pI)

26.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXPB1_D1 PF22514 57 - 179 1.6e-06 EXPB1-like domain 1
DPBB_1 PF03330 85 - 172 1.1e-18 Lytic transglycolase
Expansin_C PF01357 183 - 261 1.1e-22 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 622
Acc36I ACCTGC 1 cut(s) 622
AccB1I GGYRCC 1 cut(s) 701
AccB7I CCANNNNNTGG 2 cut(s) 226, 789
AccI GTMKAC 1 cut(s) 927
AccII CGCG 3 cut(s) 93, 441, 512
AclI AACGTT 1 cut(s) 197
AcoI YGGCCR 3 cut(s) 94, 799, 823
AcsI RAATTY 2 cut(s) 944, 1009
AdeI CACNNNGTG 1 cut(s) 226
AfaI GTAC 2 cut(s) 488, 515
AfiI CCNNNNNNNGG 2 cut(s) 226, 789
AgeI ACCGGT 1 cut(s) 698
AgsI TTSAA 9 cut(s) 233, 280, 580, 740, 812, 857, 875, 899, 1022
AhlI ACTAGT 1 cut(s) 901
AjiI CACGTC 1 cut(s) 226
AjnI CCWGG 1 cut(s) 346
AjuI GAANNNNNNNTTGG 2 cut(s) 452, 484
AluBI AGCT 7 cut(s) 23, 62, 164, 209, 611, 652, 853
AluI AGCT 7 cut(s) 23, 62, 164, 209, 611, 652, 853
Alw21I GWGCWC 1 cut(s) 25
Alw26I GTCTC 1 cut(s) 245
Ama87I CYCGRG 2 cut(s) 190, 248
AoxI GGCC 5 cut(s) 94, 148, 244, 799, 823
ApoI RAATTY 2 cut(s) 944, 1009
ArsI GACNNNNNNTTYG 2 cut(s) 244, 276
AsiGI ACCGGT 1 cut(s) 698
AspLEI GCGC 1 cut(s) 781
AspS9I GGNCC 1 cut(s) 149
AsuC2I CCSGG 1 cut(s) 565
AsuHPI GGTGA 3 cut(s) 101, 646, 728
AsuNHI GCTAGC 1 cut(s) 58
AvaI CYCGRG 2 cut(s) 190, 248
BalI TGGCCA 1 cut(s) 801
BanI GGYRCC 1 cut(s) 701
BanII GRGCYC 2 cut(s) 25, 644
BarI GAAGNNNNNNTAC 2 cut(s) 999, 1031
Bbv12I GWGCWC 1 cut(s) 25
BccI CCATC 3 cut(s) 75, 417, 703
BceAI ACGGC 1 cut(s) 838
BciT130I CCWGG 1 cut(s) 348
BcnI CCSGG 1 cut(s) 565
BcoDI GTCTC 1 cut(s) 245
BcuI ACTAGT 1 cut(s) 901
BfaI CTAG 3 cut(s) 59, 408, 902
BfuAI ACCTGC 1 cut(s) 622
BisI GCNGC 4 cut(s) 84, 94, 187, 332
BlsI GCNGC 4 cut(s) 85, 95, 188, 333
Bme1390I CCNGG 2 cut(s) 348, 565
BmeT110I CYCGRG 2 cut(s) 190, 248
BmgBI CACGTC 1 cut(s) 226
BmgT120I GGNCC 1 cut(s) 149
BmiI GGNNCC 6 cut(s) 34, 353, 525, 569, 643, 703
BmrFI CCNGG 2 cut(s) 348, 565
BmtI GCTAGC 1 cut(s) 62
BpuMI CCSGG 1 cut(s) 565
Bsa29I ATCGAT 1 cut(s) 832
BsaJI CCNNGG 6 cut(s) 36, 91, 134, 152, 347, 939
BsaWI WCCGGW 1 cut(s) 698
Bsc4I CCNNNNNNNGG 2 cut(s) 226, 789
Bse118I RCCGGY 2 cut(s) 96, 698
Bse3DI GCAATG 5 cut(s) 5, 274, 341, 477, 672
BseBI CCWGG 1 cut(s) 348
BseCI ATCGAT 1 cut(s) 832
BseDI CCNNGG 6 cut(s) 36, 91, 134, 152, 347, 939
BseGI GGATG 2 cut(s) 331, 428
BseLI CCNNNNNNNGG 2 cut(s) 226, 789
BseMI GCAATG 5 cut(s) 5, 274, 341, 477, 672
BseMII CTCAG 1 cut(s) 474
BseRI GAGGAG 1 cut(s) 621
BseX3I CGGCCG 1 cut(s) 94
BseYI CCCAGC 1 cut(s) 611
BsgI GTGCAG 1 cut(s) 650
Bsh1236I CGCG 3 cut(s) 93, 441, 512
Bsh1285I CGRYCG 1 cut(s) 97
BshFI GGCC 5 cut(s) 96, 150, 246, 801, 825
BshNI GGYRCC 1 cut(s) 701
BshTI ACCGGT 1 cut(s) 698
BshVI ATCGAT 1 cut(s) 832
BsiEI CGRYCG 1 cut(s) 97
BsiHKAI GWGCWC 1 cut(s) 25
BsiHKCI CYCGRG 2 cut(s) 190, 248
BsiSI CCGG 3 cut(s) 97, 564, 699
BslFI GGGAC 1 cut(s) 629
BslI CCNNNNNNNGG 2 cut(s) 226, 789
BsmAI GTCTC 1 cut(s) 245
BsmFI GGGAC 1 cut(s) 629
BsnI GGCC 5 cut(s) 96, 150, 246, 801, 825
BsoBI CYCGRG 2 cut(s) 190, 248
Bsp1286I GDGCHC 2 cut(s) 25, 644
Bsp143I GATC 2 cut(s) 829, 833
Bsp19I CCATGG 1 cut(s) 939
BspANI GGCC 5 cut(s) 96, 150, 246, 801, 825
BspCNI CTCAG 1 cut(s) 475
BspDI ATCGAT 1 cut(s) 832
BspFNI CGCG 3 cut(s) 93, 441, 512
BspLI GGNNCC 6 cut(s) 34, 353, 525, 569, 643, 703
BspMI ACCTGC 1 cut(s) 622
BspOI GCTAGC 1 cut(s) 62
BspT107I GGYRCC 1 cut(s) 701
BsrDI GCAATG 5 cut(s) 5, 274, 341, 477, 672
BsrFI RCCGGY 2 cut(s) 96, 698
BssAI RCCGGY 2 cut(s) 96, 698
BssECI CCNNGG 6 cut(s) 36, 91, 134, 152, 347, 939
BssMI GATC 2 cut(s) 829, 833
BssNAI GTATAC 1 cut(s) 928
BssT1I CCWWGG 4 cut(s) 36, 134, 152, 939
Bst1107I GTATAC 1 cut(s) 928
Bst2UI CCWGG 1 cut(s) 348
Bst4CI ACNGT 2 cut(s) 256, 367
BstDEI CTNAG 1 cut(s) 483
BstDSI CCRYGG 2 cut(s) 91, 939
BstEII GGTNACC 2 cut(s) 215, 694
BstF5I GGATG 2 cut(s) 331, 428
BstFNI CGCG 3 cut(s) 93, 441, 512
BstHHI GCGC 1 cut(s) 781
BstKTI GATC 2 cut(s) 832, 836
BstMAI GTCTC 1 cut(s) 245
BstMBI GATC 2 cut(s) 829, 833
BstMCI CGRYCG 1 cut(s) 97
BstMWI GCNNNNNNNGC 5 cut(s) 16, 130, 442, 778, 822
BstNI CCWGG 1 cut(s) 348
BstNSI RCATGY 4 cut(s) 306, 550, 776, 872
BstPI GGTNACC 2 cut(s) 215, 694
BstSCI CCNGG 2 cut(s) 346, 563
BstUI CGCG 3 cut(s) 93, 441, 512
BstXI CCANNNNNNTGG 1 cut(s) 396
BstZ17I GTATAC 1 cut(s) 928
BstZI CGGCCG 1 cut(s) 94
Bsu15I ATCGAT 1 cut(s) 832
BsuRI GGCC 5 cut(s) 96, 150, 246, 801, 825
BsuTUI ATCGAT 1 cut(s) 832
BtgI CCRYGG 2 cut(s) 91, 939
BtrI CACGTC 1 cut(s) 226
BtsCI GGATG 2 cut(s) 331, 428
BtsI GCAGTG 1 cut(s) 141
BtsIMutI CAGTG 4 cut(s) 141, 261, 363, 722
BveI ACCTGC 1 cut(s) 622
CfoI GCGC 1 cut(s) 781
Cfr10I RCCGGY 2 cut(s) 96, 698
Cfr13I GGNCC 1 cut(s) 149
Cfr42I CCGCGG 1 cut(s) 94
ClaI ATCGAT 1 cut(s) 832
Csp6I GTAC 2 cut(s) 487, 514
CspAI ACCGGT 1 cut(s) 698
CviQI GTAC 2 cut(s) 487, 514
DdeI CTNAG 1 cut(s) 483
DpnI GATC 2 cut(s) 831, 835
DpnII GATC 2 cut(s) 829, 833
DraIII CACNNNGTG 1 cut(s) 226
EaeI YGGCCR 3 cut(s) 94, 799, 823
EagI CGGCCG 1 cut(s) 94
EciI GGCGGA 2 cut(s) 219, 423
Ecl136II GAGCTC 1 cut(s) 23
EclXI CGGCCG 1 cut(s) 94
Eco130I CCWWGG 4 cut(s) 36, 134, 152, 939
Eco24I GRGCYC 2 cut(s) 25, 644
Eco52I CGGCCG 1 cut(s) 94
Eco53kI GAGCTC 1 cut(s) 23
Eco88I CYCGRG 2 cut(s) 190, 248
Eco91I GGTNACC 2 cut(s) 215, 694
EcoICRI GAGCTC 1 cut(s) 23
EcoO65I GGTNACC 2 cut(s) 215, 694
EcoRI GAATTC 1 cut(s) 944
EcoRII CCWGG 1 cut(s) 346
EcoT14I CCWWGG 4 cut(s) 36, 134, 152, 939
EcoT38I GRGCYC 2 cut(s) 25, 644
ErhI CCWWGG 4 cut(s) 36, 134, 152, 939
FaqI GGGAC 1 cut(s) 629
FauI CCCGC 1 cut(s) 487
FblI GTMKAC 1 cut(s) 927
Fnu4HI GCNGC 4 cut(s) 84, 94, 187, 332
FokI GGATG 2 cut(s) 338, 435
FriOI GRGCYC 2 cut(s) 25, 644
Fsp4HI GCNGC 4 cut(s) 84, 94, 187, 332
FspBI CTAG 3 cut(s) 59, 408, 902
GlaI GCGC 1 cut(s) 780
GluI GCNGC 4 cut(s) 84, 94, 187, 332
GsaI CCCAGC 1 cut(s) 615
HaeIII GGCC 5 cut(s) 96, 150, 246, 801, 825
HapII CCGG 3 cut(s) 97, 564, 699
HhaI GCGC 1 cut(s) 781
Hin6I GCGC 1 cut(s) 779
HinP1I GCGC 1 cut(s) 779
HinfI GANTC 4 cut(s) 400, 552, 670, 719
HpaII CCGG 3 cut(s) 97, 564, 699
HphI GGTGA 3 cut(s) 101, 646, 728
Hpy166II GTNNAC 3 cut(s) 109, 141, 928
Hpy188I TCNGA 4 cut(s) 534, 646, 675, 734
Hpy188III TCNNGA 4 cut(s) 192, 233, 250, 1032
Hpy8I GTNNAC 3 cut(s) 109, 141, 928
Hpy99I CGWCG 1 cut(s) 227
HpyAV CCTTC 6 cut(s) 176, 230, 631, 739, 806, 1025
HpyCH4III ACNGT 2 cut(s) 256, 367
HpyCH4IV ACGT 2 cut(s) 197, 225
HpyCH4V TGCA 6 cut(s) 302, 342, 374, 429, 631, 868
HpyF10VI GCNNNNNNNGC 5 cut(s) 16, 130, 442, 778, 822
HpyF3I CTNAG 1 cut(s) 483
HpySE526I ACGT 2 cut(s) 197, 225
HspAI GCGC 1 cut(s) 779
KspI CCGCGG 1 cut(s) 94
Kzo9I GATC 2 cut(s) 829, 833
LmnI GCTCC 3 cut(s) 206, 608, 647
MaeI CTAG 3 cut(s) 59, 408, 902
MaeII ACGT 2 cut(s) 197, 225
MaeIII GTNAC 5 cut(s) 215, 367, 410, 694, 720
MalI GATC 2 cut(s) 831, 835
MboI GATC 2 cut(s) 829, 833
MboII GAAGA 2 cut(s) 547, 1010
MfeI CAATTG 1 cut(s) 382
MhlI GDGCHC 2 cut(s) 25, 644
MlsI TGGCCA 1 cut(s) 801
MluCI AATT 4 cut(s) 382, 394, 944, 1009
MluNI TGGCCA 1 cut(s) 801
MlyI GAGTC 2 cut(s) 679, 728
MmeI TCCRAC 1 cut(s) 669
MnlI CCTC 5 cut(s) 257, 413, 482, 599, 890
Mox20I TGGCCA 1 cut(s) 801
MscI TGGCCA 1 cut(s) 801
MslI CAYNNNNRTG 2 cut(s) 72, 626
Msp20I TGGCCA 1 cut(s) 801
MspA1I CMGCKG 2 cut(s) 93, 688
MspI CCGG 3 cut(s) 97, 564, 699
MspR9I CCNGG 2 cut(s) 348, 565
MunI CAATTG 1 cut(s) 382
MvaI CCWGG 1 cut(s) 348
MvnI CGCG 3 cut(s) 93, 441, 512
MwoI GCNNNNNNNGC 5 cut(s) 16, 130, 442, 778, 822
NciI CCSGG 1 cut(s) 565
NcoI CCATGG 1 cut(s) 939
NdeII GATC 2 cut(s) 829, 833
NheI GCTAGC 1 cut(s) 58
NlaIV GGNNCC 6 cut(s) 34, 353, 525, 569, 643, 703
NmuCI GTSAC 2 cut(s) 410, 720
NspI RCATGY 4 cut(s) 306, 550, 776, 872
PaeI GCATGC 2 cut(s) 306, 550
PaeR7I CTCGAG 1 cut(s) 248
PaqCI CACCTGC 1 cut(s) 622
PfeI GAWTC 2 cut(s) 400, 552
PflMI CCANNNNNTGG 2 cut(s) 226, 789
PinAI ACCGGT 1 cut(s) 698
PkrI GCNGC 4 cut(s) 85, 95, 188, 333
PleI GAGTC 2 cut(s) 678, 727
PpsI GAGTC 2 cut(s) 678, 727
Psp124BI GAGCTC 1 cut(s) 25
Psp1406I AACGTT 1 cut(s) 197
Psp6I CCWGG 1 cut(s) 346
PspEI GGTNACC 2 cut(s) 215, 694
PspFI CCCAGC 1 cut(s) 611
PspGI CCWGG 1 cut(s) 346
PspN4I GGNNCC 6 cut(s) 34, 353, 525, 569, 643, 703
PspPI GGNCC 1 cut(s) 149
RsaI GTAC 2 cut(s) 488, 515
RsaNI GTAC 2 cut(s) 487, 514
RseI CAYNNNNRTG 2 cut(s) 72, 626
SacI GAGCTC 1 cut(s) 25
SacII CCGCGG 1 cut(s) 94
SatI GCNGC 4 cut(s) 84, 94, 187, 332
Sau3AI GATC 2 cut(s) 829, 833
Sau96I GGNCC 1 cut(s) 149
SchI GAGTC 2 cut(s) 679, 728
ScrFI CCNGG 2 cut(s) 348, 565
SduI GDGCHC 2 cut(s) 25, 644
Sfr274I CTCGAG 1 cut(s) 248
Sfr303I CCGCGG 1 cut(s) 94
SgrBI CCGCGG 1 cut(s) 94
SlaI CTCGAG 1 cut(s) 248
SmiMI CAYNNNNRTG 2 cut(s) 72, 626
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
SpeI ACTAGT 1 cut(s) 901
SphI GCATGC 2 cut(s) 306, 550
Sse9I AATT 4 cut(s) 382, 394, 944, 1009
SspI AATATT 1 cut(s) 1025
SspMI CTAG 3 cut(s) 59, 408, 902
SstI GAGCTC 1 cut(s) 25
StyD4I CCNGG 2 cut(s) 346, 563
StyI CCWWGG 4 cut(s) 36, 134, 152, 939
TaaI ACNGT 2 cut(s) 256, 367
TaiI ACGT 2 cut(s) 200, 228
TaqI TCGA 3 cut(s) 249, 450, 832
TasI AATT 4 cut(s) 382, 394, 944, 1009
TatI WGTACW 1 cut(s) 486
TauI GCSGC 4 cut(s) 86, 96, 189, 334
TfiI GAWTC 2 cut(s) 400, 552
TscAI CASTG 4 cut(s) 148, 261, 370, 729
TseFI GTSAC 2 cut(s) 410, 720
Tsp45I GTSAC 2 cut(s) 410, 720
TspDTI ATGAA 4 cut(s) 324, 329, 597, 756
TspRI CASTG 4 cut(s) 148, 261, 370, 729
Van91I CCANNNNNTGG 2 cut(s) 226, 789
XapI RAATTY 2 cut(s) 944, 1009
XceI RCATGY 4 cut(s) 306, 550, 776, 872
XhoI CTCGAG 1 cut(s) 248
XmiI GTMKAC 1 cut(s) 927
XspI CTAG 3 cut(s) 59, 408, 902
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.