pycom12g10340

expansin-A9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0004238
Physical Location & Seq
Forward (+)
12293192 .. 12295435
2244 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g10340.2

Sequence Viewer

Length: 864 bp
ATGGCATATGAAGTGCCCTTGCAGGCCCTCTTAGGGTTACTGACATTGATTTTTCTTCCTCTGGTGGCTGATGTTGTCCATGCAAGCACTGCATGGAGACTTGCAGCTAAATTATCGGAGGACGATGGACATGACATTCATACGGTTGCCCGCACCCACAACCGGCCAAGGTTCGGTGGTGGCCCTTGGAAGCATGCTCATGCCACATTCTATGAAGGCGGCCCGGGAACCTTTGGAGGAGCTTGTGGTTATGAGGATGTGATAAAAGAAGGGTATGGCCTGGACACAGCAGCGTTGAGCGAGGCTTTATTCAACAAAGGGCTGTCTTGTGGAGCATGCTATGAAATCAAATGCGTCGACAACCCTCAGTGGTGCAAGCCAGGACAGCCAACTCTCGCCGTCACGGCGACCAACAACTGCCCCCCCAATTGGAACCAGCCAAGTGACAATGGAGGGTGGTGTAACCCTCCACGTGAGCATTTTGATATAGCCAAACCTTCGTTCATCAAAATTGCCGAGTACAAGGCCGGCATTGTTCCGGTCATGTACCGCAGGGTTCCATGCCTGAAGAAAGGAGGAATTAAATTCACAATAACTGGGAATCCATATTTCAACCAAGTGTTGGTGTGGAATGTGGGAGGAGCCGGAGATGTGACCGGAATGCAAGTGAAGGGCAGCAAGAAGCTCAAATGGACAACGATGAAGCGGCTATGGGGTCAGAAGTGGGAAACTGACGCCAAGATGGTTGGCGAGTCATTGACCTTCCGAGTCAGAGCGAGTGATGGTAGATACTCAACCTCATGGCATGTTGCTCCAAGCAATTGGCAGTTTGGCCAAACCTTCGTAGGCAAGAATTTCAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0006949 GO:0008150 GO:0009653 GO:0009987 GO:0016020 GO:0030312 GO:0032502 GO:0044464 GO:0048646 GO:0048856 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

288

Amino Acids

31.64

Weight (kDa)

9.13

Isoelectric Point (pI)

26.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 622
AccI GTMKAC 1 cut(s) 357
AciI CCGC 4 cut(s) 151, 219, 550, 706
AcoI YGGCCR 2 cut(s) 164, 832
AcsI RAATTY 2 cut(s) 584, 853
AcuI CTGAAG 1 cut(s) 587
AcvI CACGTG 1 cut(s) 473
AcyI GRCGYC 1 cut(s) 735
AfaI GTAC 2 cut(s) 521, 548
AfiI CCNNNNNNNGG 5 cut(s) 33, 162, 173, 429, 622
AgsI TTSAA 2 cut(s) 313, 613
AjnI CCWGG 2 cut(s) 279, 379
AjuI GAANNNNNNNTTGG 2 cut(s) 485, 517
AluBI AGCT 3 cut(s) 107, 242, 685
AluI AGCT 3 cut(s) 107, 242, 685
Alw26I GTCTC 1 cut(s) 91
Ama87I CYCGRG 1 cut(s) 223
AoxI GGCC 7 cut(s) 24, 164, 181, 220, 277, 525, 832
ApeKI GCWGC 3 cut(s) 104, 290, 675
ApoI RAATTY 2 cut(s) 584, 853
AspS9I GGNCC 3 cut(s) 25, 182, 221
AsuC2I CCSGG 2 cut(s) 224, 225
AvaI CYCGRG 1 cut(s) 223
BaeGI GKGCMC 1 cut(s) 18
BalI TGGCCA 1 cut(s) 834
BbrPI CACGTG 1 cut(s) 473
BbvI GCAGC 3 cut(s) 116, 302, 687
BccI CCATC 3 cut(s) 119, 736, 776
BceAI ACGGC 2 cut(s) 383, 420
BciT130I CCWGG 2 cut(s) 281, 381
BcnI CCSGG 2 cut(s) 224, 225
BcoDI GTCTC 1 cut(s) 91
BglI GCCNNNNNGGC 1 cut(s) 404
BisI GCNGC 5 cut(s) 105, 220, 291, 676, 707
BlsI GCNGC 5 cut(s) 106, 221, 292, 677, 708
Bme1390I CCNGG 4 cut(s) 224, 225, 281, 381
BmeT110I CYCGRG 1 cut(s) 223
BmgT120I GGNCC 3 cut(s) 25, 182, 221
BmiI GGNNCC 4 cut(s) 229, 434, 558, 643
BmrFI CCNGG 4 cut(s) 224, 225, 281, 381
BmrI ACTGGG 1 cut(s) 606
BmuI ACTGGG 1 cut(s) 606
BpuMI CCSGG 2 cut(s) 224, 225
BsaAI YACGTR 1 cut(s) 473
BsaHI GRCGYC 1 cut(s) 735
BsaJI CCNNGG 3 cut(s) 167, 185, 223
BsaWI WCCGGW 2 cut(s) 538, 656
Bsc4I CCNNNNNNNGG 5 cut(s) 33, 162, 173, 429, 622
Bse118I RCCGGY 2 cut(s) 162, 527
Bse1I ACTGG 1 cut(s) 601
BseBI CCWGG 2 cut(s) 281, 381
BseDI CCNNGG 3 cut(s) 167, 185, 223
BseGI GGATG 1 cut(s) 262
BseLI CCNNNNNNNGG 5 cut(s) 33, 162, 173, 429, 622
BseMII CTCAG 1 cut(s) 380
BseNI ACTGG 1 cut(s) 601
BseRI GAGGAG 2 cut(s) 252, 654
BseSI GKGCMC 1 cut(s) 18
BseXI GCAGC 3 cut(s) 116, 302, 687
BshFI GGCC 7 cut(s) 26, 166, 183, 222, 279, 527, 834
BsiHKCI CYCGRG 1 cut(s) 223
BsiSI CCGG 6 cut(s) 163, 224, 528, 539, 645, 657
BslI CCNNNNNNNGG 5 cut(s) 33, 162, 173, 429, 622
BsmAI GTCTC 1 cut(s) 91
BsmI GAATGC 1 cut(s) 666
BsnI GGCC 7 cut(s) 26, 166, 183, 222, 279, 527, 834
BsoBI CYCGRG 1 cut(s) 223
Bsp1286I GDGCHC 1 cut(s) 18
BspACI CCGC 4 cut(s) 151, 219, 550, 706
BspANI GGCC 7 cut(s) 26, 166, 183, 222, 279, 527, 834
BspCNI CTCAG 1 cut(s) 379
BspLI GGNNCC 4 cut(s) 229, 434, 558, 643
BsrFI RCCGGY 2 cut(s) 162, 527
BsrI ACTGG 1 cut(s) 601
BssAI RCCGGY 2 cut(s) 162, 527
BssECI CCNNGG 3 cut(s) 167, 185, 223
BssNI GRCGYC 1 cut(s) 735
BssT1I CCWWGG 2 cut(s) 167, 185
Bst2UI CCWGG 2 cut(s) 281, 381
Bst4CI ACNGT 1 cut(s) 145
BstACI GRCGYC 1 cut(s) 735
BstAPI GCANNNNNTGC 1 cut(s) 89
BstBAI YACGTR 1 cut(s) 473
BstC8I GCNNGC 7 cut(s) 24, 85, 151, 195, 337, 377, 529
BstDEI CTNAG 2 cut(s) 31, 366
BstF5I GGATG 1 cut(s) 262
BstMAI GTCTC 1 cut(s) 91
BstMWI GCNNNNNNNGC 3 cut(s) 89, 385, 404
BstNI CCWGG 2 cut(s) 281, 381
BstNSI RCATGY 3 cut(s) 197, 339, 809
BstSCI CCNGG 4 cut(s) 222, 223, 279, 379
BstSLI GKGCMC 1 cut(s) 18
BstV1I GCAGC 3 cut(s) 116, 302, 687
BstXI CCANNNNNNTGG 1 cut(s) 822
BsuRI GGCC 7 cut(s) 26, 166, 183, 222, 279, 527, 834
BtsCI GGATG 1 cut(s) 262
BtsI GCAGTG 1 cut(s) 87
BtsIMutI CAGTG 2 cut(s) 87, 374
Cac8I GCNNGC 7 cut(s) 24, 85, 151, 195, 337, 377, 529
Cfr10I RCCGGY 2 cut(s) 162, 527
Cfr13I GGNCC 3 cut(s) 25, 182, 221
Cfr9I CCCGGG 1 cut(s) 223
CseI GACGC 2 cut(s) 343, 743
Csp6I GTAC 2 cut(s) 520, 547
CviQI GTAC 2 cut(s) 520, 547
DdeI CTNAG 2 cut(s) 31, 366
EaeI YGGCCR 2 cut(s) 164, 832
Eco130I CCWWGG 2 cut(s) 167, 185
Eco57I CTGAAG 1 cut(s) 587
Eco72I CACGTG 1 cut(s) 473
Eco88I CYCGRG 1 cut(s) 223
EcoO109I RGGNCCY 1 cut(s) 25
EcoRII CCWGG 2 cut(s) 279, 379
EcoT14I CCWWGG 2 cut(s) 167, 185
ErhI CCWWGG 2 cut(s) 167, 185
FauI CCCGC 1 cut(s) 158
FauNDI CATATG 1 cut(s) 7
FblI GTMKAC 1 cut(s) 357
Fnu4HI GCNGC 5 cut(s) 105, 220, 291, 676, 707
FokI GGATG 1 cut(s) 269
Fsp4HI GCNGC 5 cut(s) 105, 220, 291, 676, 707
GluI GCNGC 5 cut(s) 105, 220, 291, 676, 707
HaeIII GGCC 7 cut(s) 26, 166, 183, 222, 279, 527, 834
HapII CCGG 6 cut(s) 163, 224, 528, 539, 645, 657
HgaI GACGC 2 cut(s) 343, 743
Hin1I GRCGYC 1 cut(s) 735
HincII GTYRAC 1 cut(s) 358
HindII GTYRAC 1 cut(s) 358
HinfI GANTC 3 cut(s) 601, 752, 768
HpaII CCGG 6 cut(s) 163, 224, 528, 539, 645, 657
Hpy166II GTNNAC 1 cut(s) 358
Hpy188I TCNGA 4 cut(s) 118, 720, 767, 773
Hpy8I GTNNAC 1 cut(s) 358
Hpy99I CGWCG 1 cut(s) 359
HpyAV CCTTC 6 cut(s) 209, 263, 507, 664, 772, 850
HpyCH4III ACNGT 1 cut(s) 145
HpyCH4IV ACGT 1 cut(s) 472
HpyCH4V TGCA 6 cut(s) 22, 83, 92, 104, 375, 664
HpyF10VI GCNNNNNNNGC 3 cut(s) 89, 385, 404
HpyF3I CTNAG 2 cut(s) 31, 366
HpySE526I ACGT 1 cut(s) 472
Hsp92I GRCGYC 1 cut(s) 735
KroI GCCGGC 1 cut(s) 527
KroNI GCCGGC 1 cut(s) 529
LmnI GCTCC 4 cut(s) 239, 332, 641, 817
Lsp1109I GCAGC 3 cut(s) 116, 302, 687
MaeII ACGT 1 cut(s) 472
MaeIII GTNAC 5 cut(s) 36, 400, 443, 461, 652
MboII GAAGA 2 cut(s) 47, 580
MfeI CAATTG 2 cut(s) 427, 820
MhlI GDGCHC 1 cut(s) 18
MlsI TGGCCA 1 cut(s) 834
MluCI AATT 7 cut(s) 110, 427, 510, 579, 584, 820, 853
MluNI TGGCCA 1 cut(s) 834
MlyI GAGTC 2 cut(s) 761, 777
Mox20I TGGCCA 1 cut(s) 834
MroNI GCCGGC 1 cut(s) 527
MscI TGGCCA 1 cut(s) 834
MseI TTAA 1 cut(s) 582
MslI CAYNNNNRTG 1 cut(s) 198
Msp20I TGGCCA 1 cut(s) 834
MspI CCGG 6 cut(s) 163, 224, 528, 539, 645, 657
MspR9I CCNGG 4 cut(s) 224, 225, 281, 381
MunI CAATTG 2 cut(s) 427, 820
Mva1269I GAATGC 1 cut(s) 666
MvaI CCWGG 2 cut(s) 281, 381
MwoI GCNNNNNNNGC 3 cut(s) 89, 385, 404
NaeI GCCGGC 1 cut(s) 529
NciI CCSGG 2 cut(s) 224, 225
NdeI CATATG 1 cut(s) 7
NgoMIV GCCGGC 1 cut(s) 527
NlaIV GGNNCC 4 cut(s) 229, 434, 558, 643
NmeAIII GCCGAG 1 cut(s) 541
NmuCI GTSAC 3 cut(s) 400, 443, 652
NspI RCATGY 3 cut(s) 197, 339, 809
PaeI GCATGC 2 cut(s) 197, 339
PctI GAATGC 1 cut(s) 666
PdiI GCCGGC 1 cut(s) 529
PfeI GAWTC 1 cut(s) 601
PflMI CCANNNNNTGG 1 cut(s) 622
PkrI GCNGC 5 cut(s) 106, 221, 292, 677, 708
PleI GAGTC 2 cut(s) 760, 776
PmaCI CACGTG 1 cut(s) 473
PmlI CACGTG 1 cut(s) 473
PpsI GAGTC 2 cut(s) 760, 776
Ppu21I YACGTR 1 cut(s) 473
Psp6I CCWGG 2 cut(s) 279, 379
PspCI CACGTG 1 cut(s) 473
PspGI CCWGG 2 cut(s) 279, 379
PspN4I GGNNCC 4 cut(s) 229, 434, 558, 643
PspPI GGNCC 3 cut(s) 25, 182, 221
RsaI GTAC 2 cut(s) 521, 548
RsaNI GTAC 2 cut(s) 520, 547
RseI CAYNNNNRTG 1 cut(s) 198
SalI GTCGAC 1 cut(s) 356
SaqAI TTAA 1 cut(s) 582
SatI GCNGC 5 cut(s) 105, 220, 291, 676, 707
Sau96I GGNCC 3 cut(s) 25, 182, 221
SchI GAGTC 2 cut(s) 761, 777
ScrFI CCNGG 4 cut(s) 224, 225, 281, 381
SduI GDGCHC 1 cut(s) 18
SmaI CCCGGG 1 cut(s) 225
SmiMI CAYNNNNRTG 1 cut(s) 198
SphI GCATGC 2 cut(s) 197, 339
Sse9I AATT 7 cut(s) 110, 427, 510, 579, 584, 820, 853
SsiI CCGC 4 cut(s) 151, 219, 550, 706
StyD4I CCNGG 4 cut(s) 222, 223, 279, 379
StyI CCWWGG 2 cut(s) 167, 185
TaaI ACNGT 1 cut(s) 145
TaiI ACGT 1 cut(s) 475
TaqI TCGA 1 cut(s) 357
TasI AATT 7 cut(s) 110, 427, 510, 579, 584, 820, 853
TatI WGTACW 1 cut(s) 519
TauI GCSGC 2 cut(s) 222, 709
TfiI GAWTC 1 cut(s) 601
Tru1I TTAA 1 cut(s) 582
Tru9I TTAA 1 cut(s) 582
TscAI CASTG 2 cut(s) 94, 374
TseFI GTSAC 3 cut(s) 400, 443, 652
TseI GCWGC 3 cut(s) 104, 290, 675
Tsp45I GTSAC 3 cut(s) 400, 443, 652
TspDTI ATGAA 6 cut(s) 24, 128, 228, 357, 493, 716
TspMI CCCGGG 1 cut(s) 223
TspRI CASTG 2 cut(s) 94, 374
Van91I CCANNNNNTGG 1 cut(s) 622
XapI RAATTY 2 cut(s) 584, 853
XceI RCATGY 3 cut(s) 197, 339, 809
XmaI CCCGGG 1 cut(s) 223
XmiI GTMKAC 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.