Prupe.1G516600_v2.0.a1

expansin-A9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0004238
Physical Location & Seq
Forward (+)
42489506 .. 42490485
980 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G516600.1

Sequence Viewer

Length: 762 bp
ATGTTGGGTTCATTGCTGCTCTTTCTTCTAGCAGTATTTCCTGACCATGCCATTGCCCACGGTAGGCCAAGGTTCACTGCTGGCCCTTGGAAGCAAGCTCATGCCACATTCTATGAAGGAGGCTCCGGAACATTTGGTGGAGCTTGCGGTTACCATGATGTTGTTCAAGAAGGATATGGCCTGGAGACAGTAGCATTGAGCAATGCATTGTTCAACAACGGACAGTCATGTGGTGCATGCTACGATATCAAATGTGTAGACCAACCTCAATGGTGCAAGCCCGGGAGTCCGATTCTGCATGCTACGGCAACTAACAATTGCCCCCCGAATTGGAATCAGGCAAGCGACAATGGAGGATGGTGCAATCCACCACGCGAGCATTTTGACATAGCTAAGCCTGTATTCCTCAATATTGCCGAGTACAAGGCTGGAATCGTCCCAGTCGAATACCGCAGGGTTCCATGCCAAAAGAAAGGAGGAATTCGATTTACGATAACCGGCAACCCTTACTTCAACGAAGTGTTGGTTTGGAATGTGGCAGGAGCTGGAGATGTGGTCAGCGTGCAAGTGAAGGGCAACAAGTTGAAATGGACAATGATGAAGAGGATGTGGGGTCAGAGGTGGGTTACTGATGCCAAGTTGGTTGGTGAGTCACTCACCTTCCGAGTCCTAGCAAGTGATAAACGATACTCCACTTCATGGCATATAACCCCTAAGAATTGGCAGTTTGGCCAGACCTTCGAAGGCAAGAACTTCCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

254

Amino Acids

28.13

Weight (kDa)

9.1

Isoelectric Point (pI)

30.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 699
AccI GTMKAC 1 cut(s) 258
AccII CGCG 1 cut(s) 375
AccIII TCCGGA 1 cut(s) 125
AciI CCGC 2 cut(s) 147, 451
AcoI YGGCCR 1 cut(s) 730
AcsI RAATTY 1 cut(s) 480
AfaI GTAC 1 cut(s) 422
AfiI CCNNNNNNNGG 3 cut(s) 63, 330, 699
AgsI TTSAA 4 cut(s) 167, 214, 514, 586
AjnI CCWGG 1 cut(s) 180
AluBI AGCT 4 cut(s) 98, 143, 392, 545
AluI AGCT 4 cut(s) 98, 143, 392, 545
Alw26I GTCTC 1 cut(s) 179
AlwNI CAGNNNCTG 1 cut(s) 545
Ama87I CYCGRG 1 cut(s) 281
Aor13HI TCCGGA 1 cut(s) 125
AoxI GGCC 4 cut(s) 65, 82, 178, 730
ApeKI GCWGC 1 cut(s) 16
ApoI RAATTY 1 cut(s) 480
AspS9I GGNCC 1 cut(s) 83
AsuC2I CCSGG 2 cut(s) 282, 283
AsuHPI GGTGA 2 cut(s) 649, 659
AsuII TTCGAA 1 cut(s) 741
AvaI CYCGRG 1 cut(s) 281
BalI TGGCCA 1 cut(s) 732
BbvI GCAGC 1 cut(s) 3
BccI CCATC 1 cut(s) 351
BceAI ACGGC 1 cut(s) 321
BciT130I CCWGG 1 cut(s) 182
BcnI CCSGG 2 cut(s) 282, 283
BcoDI GTCTC 1 cut(s) 179
BfaI CTAG 2 cut(s) 29, 671
BisI GCNGC 1 cut(s) 17
BlpI GCTNAGC 1 cut(s) 393
BlsI GCNGC 1 cut(s) 18
Bme1390I CCNGG 3 cut(s) 182, 282, 283
BmeT110I CYCGRG 1 cut(s) 281
BmgT120I GGNCC 1 cut(s) 83
BmiI GGNNCC 2 cut(s) 124, 459
BmrFI CCNGG 3 cut(s) 182, 282, 283
BmrI ACTGGG 1 cut(s) 434
BmsI GCATC 1 cut(s) 622
BmuI ACTGGG 1 cut(s) 434
BpmI CTGGAG 2 cut(s) 203, 567
Bpu1102I GCTNAGC 1 cut(s) 393
Bpu14I TTCGAA 1 cut(s) 741
BpuMI CCSGG 2 cut(s) 282, 283
BsaJI CCNNGG 4 cut(s) 58, 68, 86, 281
BsaWI WCCGGW 1 cut(s) 125
BsaXI ACNNNNNCTCC 2 cut(s) 540, 570
Bsc4I CCNNNNNNNGG 3 cut(s) 63, 330, 699
Bse118I RCCGGY 1 cut(s) 497
Bse1I ACTGG 1 cut(s) 440
Bse3DI GCAATG 3 cut(s) 11, 51, 208
BseAI TCCGGA 1 cut(s) 125
BseBI CCWGG 1 cut(s) 182
BseDI CCNNGG 4 cut(s) 58, 68, 86, 281
BseGI GGATG 2 cut(s) 362, 612
BseLI CCNNNNNNNGG 3 cut(s) 63, 330, 699
BseMI GCAATG 3 cut(s) 11, 51, 208
BseNI ACTGG 1 cut(s) 440
BseXI GCAGC 1 cut(s) 3
Bsh1236I CGCG 1 cut(s) 375
BshFI GGCC 4 cut(s) 67, 84, 180, 732
BsiHKCI CYCGRG 1 cut(s) 281
BsiSI CCGG 3 cut(s) 126, 282, 498
BslFI GGGAC 1 cut(s) 422
BslI CCNNNNNNNGG 3 cut(s) 63, 330, 699
BsmAI GTCTC 1 cut(s) 179
BsmFI GGGAC 1 cut(s) 422
BsnI GGCC 4 cut(s) 67, 84, 180, 732
BsoBI CYCGRG 1 cut(s) 281
Bsp119I TTCGAA 1 cut(s) 741
Bsp13I TCCGGA 1 cut(s) 125
Bsp1720I GCTNAGC 1 cut(s) 393
BspACI CCGC 2 cut(s) 147, 451
BspANI GGCC 4 cut(s) 67, 84, 180, 732
BspEI TCCGGA 1 cut(s) 125
BspFNI CGCG 1 cut(s) 375
BspLI GGNNCC 2 cut(s) 124, 459
BspT104I TTCGAA 1 cut(s) 741
BsrDI GCAATG 3 cut(s) 11, 51, 208
BsrFI RCCGGY 1 cut(s) 497
BsrI ACTGG 1 cut(s) 440
BssAI RCCGGY 1 cut(s) 497
BssECI CCNNGG 4 cut(s) 58, 68, 86, 281
BssT1I CCWWGG 2 cut(s) 68, 86
Bst2UI CCWGG 1 cut(s) 182
Bst4CI ACNGT 3 cut(s) 62, 190, 225
Bst6I CTCTTC 1 cut(s) 596
BstBI TTCGAA 1 cut(s) 741
BstC8I GCNNGC 9 cut(s) 82, 96, 145, 238, 278, 300, 343, 377, 563
BstDEI CTNAG 2 cut(s) 393, 714
BstDSI CCRYGG 1 cut(s) 58
BstEII GGTNACC 1 cut(s) 149
BstF5I GGATG 2 cut(s) 362, 612
BstFNI CGCG 1 cut(s) 375
BstMAI GTCTC 1 cut(s) 179
BstNI CCWGG 1 cut(s) 182
BstNSI RCATGY 2 cut(s) 240, 302
BstPI GGTNACC 1 cut(s) 149
BstSCI CCNGG 3 cut(s) 180, 280, 281
BstUI CGCG 1 cut(s) 375
BstV1I GCAGC 1 cut(s) 3
BsuRI GGCC 4 cut(s) 67, 84, 180, 732
BtgI CCRYGG 1 cut(s) 58
BtsCI GGATG 2 cut(s) 362, 612
BtsI GCAGTG 1 cut(s) 75
BtsIMutI CAGTG 1 cut(s) 75
Cac8I GCNNGC 9 cut(s) 82, 96, 145, 238, 278, 300, 343, 377, 563
CaiI CAGNNNCTG 1 cut(s) 545
Cfr10I RCCGGY 1 cut(s) 497
Cfr13I GGNCC 1 cut(s) 83
Cfr9I CCCGGG 1 cut(s) 281
Csp6I GTAC 1 cut(s) 421
CviAII CATG 8 cut(s) 47, 101, 155, 228, 237, 299, 462, 699
CviQI GTAC 1 cut(s) 421
DdeI CTNAG 2 cut(s) 393, 714
EaeI YGGCCR 1 cut(s) 730
Eam1104I CTCTTC 1 cut(s) 596
EarI CTCTTC 1 cut(s) 596
Eco130I CCWWGG 2 cut(s) 68, 86
Eco32I GATATC 1 cut(s) 247
Eco88I CYCGRG 1 cut(s) 281
Eco91I GGTNACC 1 cut(s) 149
EcoO65I GGTNACC 1 cut(s) 149
EcoRI GAATTC 1 cut(s) 480
EcoRII CCWGG 1 cut(s) 180
EcoRV GATATC 1 cut(s) 247
EcoT14I CCWWGG 2 cut(s) 68, 86
EcoT22I ATGCAT 1 cut(s) 208
ErhI CCWWGG 2 cut(s) 68, 86
FaeI CATG 8 cut(s) 50, 104, 158, 231, 240, 302, 465, 702
FaqI GGGAC 1 cut(s) 422
FatI CATG 8 cut(s) 46, 100, 154, 227, 236, 298, 461, 698
FblI GTMKAC 1 cut(s) 258
Fnu4HI GCNGC 1 cut(s) 17
FokI GGATG 2 cut(s) 369, 619
Fsp4HI GCNGC 1 cut(s) 17
FspBI CTAG 2 cut(s) 29, 671
GluI GCNGC 1 cut(s) 17
GsuI CTGGAG 2 cut(s) 203, 567
HaeIII GGCC 4 cut(s) 67, 84, 180, 732
HapII CCGG 3 cut(s) 126, 282, 498
Hin1II CATG 8 cut(s) 50, 104, 158, 231, 240, 302, 465, 702
HinfI GANTC 6 cut(s) 286, 292, 334, 432, 650, 666
HpaII CCGG 3 cut(s) 126, 282, 498
HphI GGTGA 2 cut(s) 649, 659
Hpy166II GTNNAC 2 cut(s) 75, 259
Hpy188I TCNGA 4 cut(s) 291, 618, 665, 758
Hpy188III TCNNGA 3 cut(s) 41, 126, 167
Hpy8I GTNNAC 2 cut(s) 75, 259
HpyAV CCTTC 6 cut(s) 110, 164, 565, 670, 737, 748
HpyCH4III ACNGT 3 cut(s) 62, 190, 225
HpyCH4V TGCA 6 cut(s) 206, 236, 276, 298, 363, 565
HpyF3I CTNAG 2 cut(s) 393, 714
Hsp92II CATG 8 cut(s) 50, 104, 158, 231, 240, 302, 465, 702
Kpn2I TCCGGA 1 cut(s) 125
LmnI GCTCC 3 cut(s) 128, 140, 542
Lsp1109I GCAGC 1 cut(s) 3
LweI GCATC 1 cut(s) 622
MaeI CTAG 2 cut(s) 29, 671
MaeIII GTNAC 3 cut(s) 149, 625, 651
MboII GAAGA 2 cut(s) 17, 613
MfeI CAATTG 1 cut(s) 316
MlsI TGGCCA 1 cut(s) 732
MluCI AATT 4 cut(s) 316, 328, 480, 718
MluNI TGGCCA 1 cut(s) 732
MlyI GAGTC 3 cut(s) 295, 659, 675
MnlI CCTC 7 cut(s) 113, 276, 347, 416, 470, 597, 612
Mox20I TGGCCA 1 cut(s) 732
Mph1103I ATGCAT 1 cut(s) 208
MroI TCCGGA 1 cut(s) 125
MscI TGGCCA 1 cut(s) 732
Msp20I TGGCCA 1 cut(s) 732
MspI CCGG 3 cut(s) 126, 282, 498
MspR9I CCNGG 3 cut(s) 182, 282, 283
MunI CAATTG 1 cut(s) 316
MvaI CCWGG 1 cut(s) 182
MvnI CGCG 1 cut(s) 375
NciI CCSGG 2 cut(s) 282, 283
NlaIII CATG 8 cut(s) 50, 104, 158, 231, 240, 302, 465, 702
NlaIV GGNNCC 2 cut(s) 124, 459
NmeAIII GCCGAG 1 cut(s) 442
NmuCI GTSAC 1 cut(s) 651
NsiI ATGCAT 1 cut(s) 208
NspI RCATGY 2 cut(s) 240, 302
NspV TTCGAA 1 cut(s) 741
PaeI GCATGC 2 cut(s) 240, 302
PcsI WCGNNNNNNNCGW 1 cut(s) 441
PfeI GAWTC 3 cut(s) 292, 334, 432
PflMI CCANNNNNTGG 1 cut(s) 699
PkrI GCNGC 1 cut(s) 18
PleI GAGTC 3 cut(s) 294, 658, 674
PpsI GAGTC 3 cut(s) 294, 658, 674
Psp6I CCWGG 1 cut(s) 180
PspEI GGTNACC 1 cut(s) 149
PspGI CCWGG 1 cut(s) 180
PspN4I GGNNCC 2 cut(s) 124, 459
PspPI GGNCC 1 cut(s) 83
PstNI CAGNNNCTG 1 cut(s) 545
RsaI GTAC 1 cut(s) 422
RsaNI GTAC 1 cut(s) 421
SatI GCNGC 1 cut(s) 17
Sau96I GGNCC 1 cut(s) 83
SchI GAGTC 3 cut(s) 295, 659, 675
ScrFI CCNGG 3 cut(s) 182, 282, 283
SetI ASST 9 cut(s) 74, 100, 145, 268, 394, 547, 623, 662, 740
SfaNI GCATC 1 cut(s) 622
SfuI TTCGAA 1 cut(s) 741
SmaI CCCGGG 1 cut(s) 283
SphI GCATGC 2 cut(s) 240, 302
Sse9I AATT 4 cut(s) 316, 328, 480, 718
SsiI CCGC 2 cut(s) 147, 451
SspI AATATT 1 cut(s) 412
SspMI CTAG 2 cut(s) 29, 671
StyD4I CCNGG 3 cut(s) 180, 280, 281
StyI CCWWGG 2 cut(s) 68, 86
TaaI ACNGT 3 cut(s) 62, 190, 225
TaqI TCGA 3 cut(s) 444, 484, 741
TasI AATT 4 cut(s) 316, 328, 480, 718
TatI WGTACW 1 cut(s) 420
TfiI GAWTC 3 cut(s) 292, 334, 432
TscAI CASTG 1 cut(s) 82
TseFI GTSAC 1 cut(s) 651
TseI GCWGC 1 cut(s) 16
Tsp45I GTSAC 1 cut(s) 651
TspDTI ATGAA 3 cut(s) 129, 614, 687
TspGWI ACGGA 1 cut(s) 234
TspMI CCCGGG 1 cut(s) 281
TspRI CASTG 1 cut(s) 82
Van91I CCANNNNNTGG 1 cut(s) 699
XapI RAATTY 1 cut(s) 480
XceI RCATGY 2 cut(s) 240, 302
XmaI CCCGGG 1 cut(s) 281
XmiI GTMKAC 1 cut(s) 258
XspI CTAG 2 cut(s) 29, 671
Zsp2I ATGCAT 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.