Rroxscaffold_6G00410400

expansin-A9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0004238
Physical Location & Seq
Forward (+)
32835610 .. 32836747
1138 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00410400.1

Sequence Viewer

Length: 690 bp
ATGTCAATGGCGTCAATGAGTTCTGCCCTCCTGGGTTCATTGCTTTTCATTCTTCTCGTGTTACCTGACCATGTCACCGCTCGCCATAAGCCAAAGTTCAGACCTGGTCCTTGGAAGCATGCTCATGCCACATTCTACGAAGGCGGCTCTGGATCATTTGGTGGTGCTTGTGGTTACCACGACGTCGTTCAAGAAGGCTATGGCCTCGACACGGTGGCTTTGAGCGACACCTTCAACCAATCATTGCCCCCGAATTATAACCTTCCCAGTGACAATGGAGGATGGTGCAATGCACCACGTGAGCACTTCGATATAGCCAAGCCGGTGTTCCTCAACATTGCAGAGTATAAGGCTGGCATAGTACCAATCATATACCGCAGGGTTCCGTGCCAAAAGAGAGGAGGCATCCGATTCACGATAACCGGGAATCCCTACTTCAATGAAGTGTTGGTGTGGAACGTGGGAGGTGCCGGAGATGTGATAAGCGTGCAAGTGAAGGGCCATGACAAGCTCAAATGGACAACGATGAAGCGAATGTGGGGTCAGAGGTGGATTACTGATGCCAAGTTGGTTGGGGAGTCTCTGACCTTCAGAGTTGAAGCAAGTGATAAAAGACGCTCTACTTCTTGGCACATAGCCCCTCAGAACTGGCAGTTTGGCCAGACCTTTGAAGGCAAGAACTTCCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

229

Amino Acids

25.64

Weight (kDa)

9.49

Isoelectric Point (pI)

42.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Expansin_C PF01357 136 - 214 4.8e-24 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 258
AatII GACGTC 1 cut(s) 186
AccB1I GGYRCC 1 cut(s) 467
AccBSI CCGCTC 1 cut(s) 80
AciI CCGC 3 cut(s) 78, 144, 376
AclWI GGATC 1 cut(s) 160
AcoI YGGCCR 1 cut(s) 658
AcuI CTGAAG 1 cut(s) 574
AcvI CACGTG 1 cut(s) 299
AcyI GRCGYC 2 cut(s) 11, 183
AdeI CACNNNGTG 1 cut(s) 299
AfaI GTAC 1 cut(s) 363
AfiI CCNNNNNNNGG 1 cut(s) 211
AgsI TTSAA 5 cut(s) 191, 235, 439, 599, 671
AjnI CCWGG 2 cut(s) 30, 103
AjuI GAANNNNNNNTTGG 2 cut(s) 311, 343
AluBI AGCT 1 cut(s) 511
AluI AGCT 1 cut(s) 511
Alw21I GWGCWC 1 cut(s) 306
Alw26I GTCTC 1 cut(s) 585
AlwI GGATC 1 cut(s) 160
AoxI GGCC 3 cut(s) 202, 499, 658
ArsI GACNNNNNNTTYG 2 cut(s) 526, 558
AspS9I GGNCC 2 cut(s) 107, 499
AsuC2I CCSGG 1 cut(s) 424
AsuHPI GGTGA 1 cut(s) 67
AvaII GGWCC 1 cut(s) 107
BalI TGGCCA 1 cut(s) 660
BanI GGYRCC 1 cut(s) 467
BauI CACGAG 1 cut(s) 56
BbrPI CACGTG 1 cut(s) 299
Bbv12I GWGCWC 1 cut(s) 306
BccI CCATC 1 cut(s) 276
BciT130I CCWGG 2 cut(s) 32, 105
BcnI CCSGG 1 cut(s) 424
BcoDI GTCTC 1 cut(s) 585
BisI GCNGC 1 cut(s) 145
BlsI GCNGC 1 cut(s) 146
Bme1390I CCNGG 3 cut(s) 32, 105, 424
Bme18I GGWCC 1 cut(s) 107
BmgT120I GGNCC 2 cut(s) 107, 499
BmiI GGNNCC 2 cut(s) 384, 469
BmrFI CCNGG 3 cut(s) 32, 105, 424
BmrI ACTGGG 1 cut(s) 261
BmsI GCATC 2 cut(s) 414, 550
BmuI ACTGGG 1 cut(s) 261
BpuMI CCSGG 1 cut(s) 424
BsaAI YACGTR 1 cut(s) 299
BsaHI GRCGYC 2 cut(s) 11, 183
BsaJI CCNNGG 2 cut(s) 31, 110
Bsc4I CCNNNNNNNGG 1 cut(s) 211
Bse118I RCCGGY 1 cut(s) 322
Bse1I ACTGG 2 cut(s) 267, 653
Bse3DI GCAATG 4 cut(s) 38, 242, 295, 336
BseBI CCWGG 2 cut(s) 32, 105
BseDI CCNNGG 2 cut(s) 31, 110
BseGI GGATG 2 cut(s) 287, 405
BseLI CCNNNNNNNGG 1 cut(s) 211
BseMI GCAATG 4 cut(s) 38, 242, 295, 336
BseMII CTCAG 1 cut(s) 656
BseNI ACTGG 2 cut(s) 267, 653
BseRI GAGGAG 1 cut(s) 414
BshFI GGCC 3 cut(s) 204, 501, 660
BshNI GGYRCC 1 cut(s) 467
BsiHKAI GWGCWC 1 cut(s) 306
BsiSI CCGG 3 cut(s) 323, 423, 471
BslI CCNNNNNNNGG 1 cut(s) 211
BsmAI GTCTC 1 cut(s) 585
BsnI GGCC 3 cut(s) 204, 501, 660
Bsp1286I GDGCHC 1 cut(s) 306
Bsp143I GATC 1 cut(s) 152
BspACI CCGC 3 cut(s) 78, 144, 376
BspANI GGCC 3 cut(s) 204, 501, 660
BspCNI CTCAG 1 cut(s) 655
BspLI GGNNCC 2 cut(s) 384, 469
BspPI GGATC 1 cut(s) 160
BspT107I GGYRCC 1 cut(s) 467
BsrBI CCGCTC 1 cut(s) 80
BsrDI GCAATG 4 cut(s) 38, 242, 295, 336
BsrFI RCCGGY 1 cut(s) 322
BsrI ACTGG 2 cut(s) 267, 653
BssAI RCCGGY 1 cut(s) 322
BssECI CCNNGG 2 cut(s) 31, 110
BssMI GATC 1 cut(s) 152
BssNI GRCGYC 2 cut(s) 11, 183
BssSI CACGAG 1 cut(s) 56
BssT1I CCWWGG 1 cut(s) 110
Bst2BI CACGAG 1 cut(s) 56
Bst2UI CCWGG 2 cut(s) 32, 105
Bst4CI ACNGT 1 cut(s) 214
BstACI GRCGYC 2 cut(s) 11, 183
BstBAI YACGTR 1 cut(s) 299
BstC8I GCNNGC 4 cut(s) 82, 120, 355, 488
BstDEI CTNAG 1 cut(s) 642
BstEII GGTNACC 1 cut(s) 173
BstF5I GGATG 2 cut(s) 287, 405
BstKTI GATC 1 cut(s) 155
BstMAI GTCTC 1 cut(s) 585
BstMBI GATC 1 cut(s) 152
BstNI CCWGG 2 cut(s) 32, 105
BstNSI RCATGY 1 cut(s) 122
BstPI GGTNACC 1 cut(s) 173
BstSCI CCNGG 3 cut(s) 30, 103, 422
BsuRI GGCC 3 cut(s) 204, 501, 660
BtsCI GGATG 2 cut(s) 287, 405
BtsIMutI CAGTG 1 cut(s) 274
Cac8I GCNNGC 4 cut(s) 82, 120, 355, 488
Cfr10I RCCGGY 1 cut(s) 322
Cfr13I GGNCC 2 cut(s) 107, 499
CseI GACGC 1 cut(s) 624
CsiI ACCWGGT 1 cut(s) 103
Csp6I GTAC 1 cut(s) 362
CviAII CATG 4 cut(s) 71, 119, 125, 503
CviQI GTAC 1 cut(s) 362
DdeI CTNAG 1 cut(s) 642
DpnI GATC 1 cut(s) 154
DpnII GATC 1 cut(s) 152
DraIII CACNNNGTG 1 cut(s) 299
EaeI YGGCCR 1 cut(s) 658
Eco130I CCWWGG 1 cut(s) 110
Eco47I GGWCC 1 cut(s) 107
Eco57I CTGAAG 1 cut(s) 574
Eco72I CACGTG 1 cut(s) 299
Eco91I GGTNACC 1 cut(s) 173
EcoO65I GGTNACC 1 cut(s) 173
EcoRII CCWGG 2 cut(s) 30, 103
EcoT14I CCWWGG 1 cut(s) 110
ErhI CCWWGG 1 cut(s) 110
FaeI CATG 4 cut(s) 74, 122, 128, 506
FatI CATG 4 cut(s) 70, 118, 124, 502
Fnu4HI GCNGC 1 cut(s) 145
FokI GGATG 2 cut(s) 294, 392
Fsp4HI GCNGC 1 cut(s) 145
GluI GCNGC 1 cut(s) 145
HaeIII GGCC 3 cut(s) 204, 501, 660
HapII CCGG 3 cut(s) 323, 423, 471
HgaI GACGC 1 cut(s) 624
Hin1I GRCGYC 2 cut(s) 11, 183
Hin1II CATG 4 cut(s) 74, 122, 128, 506
HinfI GANTC 3 cut(s) 411, 427, 578
HpaII CCGG 3 cut(s) 323, 423, 471
HphI GGTGA 1 cut(s) 67
Hpy188I TCNGA 7 cut(s) 101, 410, 546, 585, 593, 645, 686
Hpy188III TCNNGA 3 cut(s) 150, 191, 415
Hpy99I CGWCG 2 cut(s) 185, 188
HpyAV CCTTC 7 cut(s) 134, 188, 241, 272, 490, 598, 665
HpyCH4III ACNGT 1 cut(s) 214
HpyCH4IV ACGT 3 cut(s) 183, 298, 459
HpyCH4V TGCA 4 cut(s) 288, 293, 341, 490
HpyF3I CTNAG 1 cut(s) 642
HpySE526I ACGT 3 cut(s) 183, 298, 459
Hsp92I GRCGYC 2 cut(s) 11, 183
Hsp92II CATG 4 cut(s) 74, 122, 128, 506
Kzo9I GATC 1 cut(s) 152
LweI GCATC 2 cut(s) 414, 550
MabI ACCWGGT 1 cut(s) 103
MaeII ACGT 3 cut(s) 183, 298, 459
MaeIII GTNAC 4 cut(s) 60, 73, 173, 269
MalI GATC 1 cut(s) 154
MbiI CCGCTC 1 cut(s) 80
MboI GATC 1 cut(s) 152
MboII GAAGA 1 cut(s) 44
MhlI GDGCHC 1 cut(s) 306
MlsI TGGCCA 1 cut(s) 660
MluCI AATT 1 cut(s) 253
MluNI TGGCCA 1 cut(s) 660
MlyI GAGTC 1 cut(s) 587
MnlI CCTC 9 cut(s) 38, 215, 272, 341, 392, 395, 458, 540, 651
Mox20I TGGCCA 1 cut(s) 660
MscI TGGCCA 1 cut(s) 660
MslI CAYNNNNRTG 1 cut(s) 123
Msp20I TGGCCA 1 cut(s) 660
MspI CCGG 3 cut(s) 323, 423, 471
MspR9I CCNGG 3 cut(s) 32, 105, 424
MvaI CCWGG 2 cut(s) 32, 105
NciI CCSGG 1 cut(s) 424
NdeII GATC 1 cut(s) 152
NlaIII CATG 4 cut(s) 74, 122, 128, 506
NlaIV GGNNCC 2 cut(s) 384, 469
NmuCI GTSAC 2 cut(s) 73, 269
NspI RCATGY 1 cut(s) 122
PaeI GCATGC 1 cut(s) 122
PfeI GAWTC 2 cut(s) 411, 427
PflFI GACNNNGTC 2 cut(s) 71, 105
PkrI GCNGC 1 cut(s) 146
PleI GAGTC 1 cut(s) 586
PmaCI CACGTG 1 cut(s) 299
PmlI CACGTG 1 cut(s) 299
PpsI GAGTC 1 cut(s) 586
Ppu21I YACGTR 1 cut(s) 299
PsiI TTATAA 1 cut(s) 258
Psp6I CCWGG 2 cut(s) 30, 103
PspCI CACGTG 1 cut(s) 299
PspEI GGTNACC 1 cut(s) 173
PspGI CCWGG 2 cut(s) 30, 103
PspN4I GGNNCC 2 cut(s) 384, 469
PspPI GGNCC 2 cut(s) 107, 499
PsyI GACNNNGTC 2 cut(s) 71, 105
RsaI GTAC 1 cut(s) 363
RsaNI GTAC 1 cut(s) 362
RseI CAYNNNNRTG 1 cut(s) 123
SatI GCNGC 1 cut(s) 145
Sau3AI GATC 1 cut(s) 152
Sau96I GGNCC 2 cut(s) 107, 499
SchI GAGTC 1 cut(s) 587
ScrFI CCNGG 3 cut(s) 32, 105, 424
SduI GDGCHC 1 cut(s) 306
SexAI ACCWGGT 1 cut(s) 103
SfaNI GCATC 2 cut(s) 414, 550
SinI GGWCC 1 cut(s) 107
SmiMI CAYNNNNRTG 1 cut(s) 123
SphI GCATGC 1 cut(s) 122
Sse9I AATT 1 cut(s) 253
SsiI CCGC 3 cut(s) 78, 144, 376
StyD4I CCNGG 3 cut(s) 30, 103, 422
StyI CCWWGG 1 cut(s) 110
TaaI ACNGT 1 cut(s) 214
TaiI ACGT 3 cut(s) 186, 301, 462
TaqI TCGA 2 cut(s) 207, 309
TasI AATT 1 cut(s) 253
TauI GCSGC 1 cut(s) 147
TfiI GAWTC 2 cut(s) 411, 427
TscAI CASTG 1 cut(s) 274
TseFI GTSAC 2 cut(s) 73, 269
Tsp45I GTSAC 2 cut(s) 73, 269
TspDTI ATGAA 4 cut(s) 27, 37, 456, 542
TspGWI ACGGA 1 cut(s) 375
TspRI CASTG 1 cut(s) 274
Tth111I GACNNNGTC 2 cut(s) 71, 105
VpaK11BI GGWCC 1 cut(s) 107
XceI RCATGY 1 cut(s) 122
ZraI GACGTC 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.