Rorug03G0114000

expansin-A9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0004238
Physical Location & Seq
Reverse (-)
9187024 .. 9191396
4373 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0114000.1

Sequence Viewer

Length: 1128 bp
ATGGCGTCCGTACTCAATACGGTGCCGTCCGCTAGATTTTCGAACCCTAACGCCAACCGAGTTCGTACTCACTCCGTTTTGGCGCCGATTTCCGTTTCGTTTTCCAGGAGGAGATTTACAGTTAGGGCTGCAGAGACTGATACGAATGAAGAAATTAAATCTCAGGCGGCACCGGATAAGGCACCGGCGAGCGGTGGTTCGAGCTTTAATCAGCTTCTTGGAATCAAAGGAGCTTCTCAAGAAACTAATAAATGGAAGATTCGTCTTCAGCTAACCAAACCTGTTACTTGGCCTCCTTTGGTATGGGGAGTAGTTTGCGGAGCTGCTGCCTCTGGAAATTTTCATTGGAATTTGGAGGATGTTGCCAAATCTATACTTTGTATGTCAATGTCTGGCCCTTTTCTCACTGGCTACACACAGACACTAAATGATTGGTATGACCGAGAGATTGATGCAATCAATGAGCCATATCGTCCAATTCCCTCGGGGGCAATATCTGAGAATGAGGTTATTACTCAAATCTGGGTACTGCTTTTAGGAGGTCTTGTCTCGGCTGGTATATTGGATGTGTGGGCAGGACATGATTTCCCAATAGTCTTTTACCTTGCTCTGGGTGGATCCTTGCTATCGTACATTTACTCTGCTCCACCTCTAAAGCTTAAACAAAATGGATGGATTGGAAATTTTGCGCTTGGAGCAAGTTATATCGGTTTGCCATGGTGGGCTGGTCAGGCATTATTTGGAACCCTTACACCTGACATAATTGTCCTCACACTCCTGTACAGCATTGCCGGATTGGGGATTGCTATTGTAAATGACTTCAAAAGTGTTGAAGGAGATAGAGCACTGGGACTTCAGTCACTCCCCGTAGCTTTTGGTTCTGAAACTGCAAAATGGATTTGCGTGGGTGCCATCGACATAACTCAGCTATCGATTGCTGGTTATCTGCTAGGGGCTGGTAAACCATACTATGCATTAGCTCTAGTTGCTTTGATAGCTCCCCAAGTCTTTTTCCAGTTCAAGTATTTTCTCAAAGACCCTGTCAAGTATGATGTTAAATATCAGGCCAGTGCGCAGCCATTTCTTGTACTTGGTCTTCTGGTAACAGCTTTAGCAACCAGTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

375

Amino Acids

40.53

Weight (kDa)

8.29

Isoelectric Point (pI)

28.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UbiA PF01040 98 - 346 1.3e-38 UbiA prenyltransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 764
Acc16I TGCGCA 1 cut(s) 1074
AccB1I GGYRCC 5 cut(s) 22, 82, 169, 181, 908
AccBSI CCGCTC 1 cut(s) 192
AciI CCGC 4 cut(s) 30, 167, 192, 318
AclWI GGATC 2 cut(s) 612, 625
AcsI RAATTY 3 cut(s) 337, 349, 682
AcuI CTGAAG 2 cut(s) 251, 839
AcyI GRCGYC 2 cut(s) 5, 83
AfaI GTAC 6 cut(s) 12, 67, 528, 632, 782, 1089
AfiI CCNNNNNNNGG 3 cut(s) 191, 610, 798
AgsI TTSAA 3 cut(s) 823, 833, 1021
AjnI CCWGG 1 cut(s) 104
AjuI GAANNNNNNNTTGG 2 cut(s) 328, 360
Alw21I GWGCWC 1 cut(s) 847
Alw26I GTCTC 2 cut(s) 128, 553
AlwI GGATC 2 cut(s) 612, 625
AlwNI CAGNNNCTG 1 cut(s) 137
Ama87I CYCGRG 1 cut(s) 484
AoxI GGCC 3 cut(s) 290, 394, 1065
ApeKI GCWGC 4 cut(s) 128, 323, 326, 1075
ApoI RAATTY 3 cut(s) 337, 349, 682
ArsI GACNNNNNNTTYG 2 cut(s) 1026, 1058
AspLEI GCGC 3 cut(s) 85, 691, 1075
AspS9I GGNCC 1 cut(s) 395
AsuII TTCGAA 1 cut(s) 41
AvaI CYCGRG 1 cut(s) 484
BamHI GGATCC 1 cut(s) 617
BanI GGYRCC 5 cut(s) 22, 82, 169, 181, 908
BarI GAAGNNNNNNTAC 2 cut(s) 1080, 1112
BbsI GAAGAC 2 cut(s) 257, 1088
Bbv12I GWGCWC 1 cut(s) 847
BbvI GCAGC 4 cut(s) 115, 310, 313, 1087
BccI CCATC 2 cut(s) 666, 920
BceAI ACGGC 1 cut(s) 10
BciT130I CCWGG 1 cut(s) 106
BcoDI GTCTC 2 cut(s) 128, 553
BfaI CTAG 3 cut(s) 33, 950, 983
BfmI CTRYAG 1 cut(s) 129
BfoI RGCGCY 1 cut(s) 86
BisI GCNGC 5 cut(s) 129, 168, 324, 327, 1076
BlsI GCNGC 5 cut(s) 130, 169, 325, 328, 1077
Bme1390I CCNGG 1 cut(s) 106
BmeT110I CYCGRG 1 cut(s) 484
BmgT120I GGNCC 1 cut(s) 395
BmiI GGNNCC 7 cut(s) 24, 84, 171, 183, 619, 745, 910
BmrFI CCNGG 1 cut(s) 106
BmrI ACTGGG 1 cut(s) 857
BmsI GCATC 1 cut(s) 442
BmuI ACTGGG 1 cut(s) 857
BoxI GACNNNNGTC 1 cut(s) 856
BpiI GAAGAC 2 cut(s) 257, 1088
Bpu14I TTCGAA 1 cut(s) 41
BpuEI CTTGAG 1 cut(s) 222
Bsa29I ATCGAT 1 cut(s) 932
BsaHI GRCGYC 2 cut(s) 5, 83
BsaJI CCNNGG 2 cut(s) 483, 716
BsaWI WCCGGW 1 cut(s) 172
BsaXI ACNNNNNCTCC 4 cut(s) 277, 307, 531, 561
Bsc4I CCNNNNNNNGG 3 cut(s) 191, 610, 798
Bse118I RCCGGY 1 cut(s) 184
Bse1I ACTGG 5 cut(s) 412, 852, 1015, 1068, 1119
Bse3DI GCAATG 1 cut(s) 786
BseBI CCWGG 1 cut(s) 106
BseCI ATCGAT 1 cut(s) 932
BseDI CCNNGG 2 cut(s) 483, 716
BseGI GGATG 3 cut(s) 364, 571, 677
BseLI CCNNNNNNNGG 3 cut(s) 191, 610, 798
BseMI GCAATG 1 cut(s) 786
BseMII CTCAG 3 cut(s) 176, 489, 938
BseNI ACTGG 5 cut(s) 412, 852, 1015, 1068, 1119
BseRI GAGGAG 1 cut(s) 124
BseXI GCAGC 4 cut(s) 115, 310, 313, 1087
BshFI GGCC 3 cut(s) 292, 396, 1067
BshNI GGYRCC 5 cut(s) 22, 82, 169, 181, 908
BshVI ATCGAT 1 cut(s) 932
BsiHKAI GWGCWC 1 cut(s) 847
BsiHKCI CYCGRG 1 cut(s) 484
BsiSI CCGG 3 cut(s) 173, 185, 792
BslFI GGGAC 1 cut(s) 864
BslI CCNNNNNNNGG 3 cut(s) 191, 610, 798
BsmAI GTCTC 2 cut(s) 128, 553
BsmFI GGGAC 1 cut(s) 864
BsnI GGCC 3 cut(s) 292, 396, 1067
BsoBI CYCGRG 1 cut(s) 484
Bsp119I TTCGAA 1 cut(s) 41
Bsp1286I GDGCHC 1 cut(s) 847
Bsp1407I TGTACA 1 cut(s) 780
Bsp143I GATC 1 cut(s) 617
Bsp19I CCATGG 1 cut(s) 716
BspACI CCGC 4 cut(s) 30, 167, 192, 318
BspANI GGCC 3 cut(s) 292, 396, 1067
BspCNI CTCAG 3 cut(s) 175, 490, 937
BspDI ATCGAT 1 cut(s) 932
BspLI GGNNCC 7 cut(s) 24, 84, 171, 183, 619, 745, 910
BspMAI CTGCAG 1 cut(s) 133
BspPI GGATC 2 cut(s) 612, 625
BspT104I TTCGAA 1 cut(s) 41
BspT107I GGYRCC 5 cut(s) 22, 82, 169, 181, 908
BsrBI CCGCTC 1 cut(s) 192
BsrDI GCAATG 1 cut(s) 786
BsrFI RCCGGY 1 cut(s) 184
BsrGI TGTACA 1 cut(s) 780
BsrI ACTGG 5 cut(s) 412, 852, 1015, 1068, 1119
BssAI RCCGGY 1 cut(s) 184
BssECI CCNNGG 2 cut(s) 483, 716
BssMI GATC 1 cut(s) 617
BssNI GRCGYC 2 cut(s) 5, 83
BssT1I CCWWGG 1 cut(s) 716
Bst2UI CCWGG 1 cut(s) 106
Bst4CI ACNGT 2 cut(s) 22, 121
BstACI GRCGYC 2 cut(s) 5, 83
BstAUI TGTACA 1 cut(s) 780
BstBI TTCGAA 1 cut(s) 41
BstC8I GCNNGC 1 cut(s) 190
BstDEI CTNAG 3 cut(s) 162, 498, 924
BstDSI CCRYGG 1 cut(s) 716
BstF5I GGATG 3 cut(s) 364, 571, 677
BstH2I RGCGCY 1 cut(s) 86
BstHHI GCGC 3 cut(s) 85, 691, 1075
BstKTI GATC 1 cut(s) 620
BstMAI GTCTC 2 cut(s) 128, 553
BstMBI GATC 1 cut(s) 617
BstMWI GCNNNNNNNGC 4 cut(s) 695, 731, 986, 995
BstNI CCWGG 1 cut(s) 106
BstPAI GACNNNNGTC 1 cut(s) 856
BstSCI CCNGG 1 cut(s) 104
BstSFI CTRYAG 1 cut(s) 129
BstV1I GCAGC 4 cut(s) 115, 310, 313, 1087
BstV2I GAAGAC 2 cut(s) 257, 1088
BstX2I RGATCY 1 cut(s) 617
BstYI RGATCY 1 cut(s) 617
Bsu15I ATCGAT 1 cut(s) 932
BsuRI GGCC 3 cut(s) 292, 396, 1067
BsuTUI ATCGAT 1 cut(s) 932
BtgI CCRYGG 1 cut(s) 716
BtsCI GGATG 3 cut(s) 364, 571, 677
BtsIMutI CAGTG 3 cut(s) 405, 845, 1075
Cac8I GCNNGC 1 cut(s) 190
CaiI CAGNNNCTG 1 cut(s) 137
CfoI GCGC 3 cut(s) 85, 691, 1075
Cfr10I RCCGGY 1 cut(s) 184
Cfr13I GGNCC 1 cut(s) 395
ClaI ATCGAT 1 cut(s) 932
Csp6I GTAC 6 cut(s) 11, 66, 527, 631, 781, 1088
CviAII CATG 2 cut(s) 581, 717
CviQI GTAC 6 cut(s) 11, 66, 527, 631, 781, 1088
DdeI CTNAG 3 cut(s) 162, 498, 924
DinI GGCGCC 1 cut(s) 84
DpnI GATC 1 cut(s) 619
DpnII GATC 1 cut(s) 617
DrdI GACNNNNNNGTC 1 cut(s) 764
DseDI GACNNNNNNGTC 1 cut(s) 764
Eco130I CCWWGG 1 cut(s) 716
Eco57I CTGAAG 2 cut(s) 251, 839
Eco88I CYCGRG 1 cut(s) 484
EcoRII CCWGG 1 cut(s) 104
EcoT14I CCWWGG 1 cut(s) 716
EcoT22I ATGCAT 1 cut(s) 976
EgeI GGCGCC 1 cut(s) 84
EheI GGCGCC 1 cut(s) 84
ErhI CCWWGG 1 cut(s) 716
FaeI CATG 2 cut(s) 584, 720
FaqI GGGAC 1 cut(s) 864
FatI CATG 2 cut(s) 580, 716
Fnu4HI GCNGC 5 cut(s) 129, 168, 324, 327, 1076
FokI GGATG 3 cut(s) 371, 578, 684
Fsp4HI GCNGC 5 cut(s) 129, 168, 324, 327, 1076
FspBI CTAG 3 cut(s) 33, 950, 983
FspI TGCGCA 1 cut(s) 1074
GlaI GCGC 3 cut(s) 84, 690, 1074
GluI GCNGC 5 cut(s) 129, 168, 324, 327, 1076
HaeII RGCGCY 1 cut(s) 86
HaeIII GGCC 3 cut(s) 292, 396, 1067
HapII CCGG 3 cut(s) 173, 185, 792
HhaI GCGC 3 cut(s) 85, 691, 1075
Hin1I GRCGYC 2 cut(s) 5, 83
Hin1II CATG 2 cut(s) 584, 720
Hin6I GCGC 3 cut(s) 83, 689, 1073
HinP1I GCGC 3 cut(s) 83, 689, 1073
HindIII AAGCTT 1 cut(s) 656
HinfI GANTC 2 cut(s) 222, 259
HpaII CCGG 3 cut(s) 173, 185, 792
Hpy166II GTNNAC 1 cut(s) 962
Hpy188I TCNGA 2 cut(s) 499, 883
Hpy188III TCNNGA 2 cut(s) 239, 333
Hpy8I GTNNAC 1 cut(s) 962
HpyAV CCTTC 1 cut(s) 827
HpyCH4III ACNGT 2 cut(s) 22, 121
HpyCH4V TGCA 4 cut(s) 131, 455, 890, 974
HpyF10VI GCNNNNNNNGC 4 cut(s) 695, 731, 986, 995
HpyF3I CTNAG 3 cut(s) 162, 498, 924
Hsp92I GRCGYC 2 cut(s) 5, 83
Hsp92II CATG 2 cut(s) 584, 720
HspAI GCGC 3 cut(s) 83, 689, 1073
KasI GGCGCC 1 cut(s) 82
Kzo9I GATC 1 cut(s) 617
LmnI GCTCC 5 cut(s) 230, 320, 649, 695, 1003
Lsp1109I GCAGC 4 cut(s) 115, 310, 313, 1087
LweI GCATC 1 cut(s) 442
MaeI CTAG 3 cut(s) 33, 950, 983
MaeIII GTNAC 3 cut(s) 283, 858, 1102
MalI GATC 1 cut(s) 619
MbiI CCGCTC 1 cut(s) 192
MboI GATC 1 cut(s) 617
MboII GAAGA 4 cut(s) 161, 257, 268, 1088
MflI RGATCY 1 cut(s) 617
MhlI GDGCHC 1 cut(s) 847
MluCI AATT 6 cut(s) 153, 337, 349, 477, 682, 762
Mly113I GGCGCC 1 cut(s) 83
MnlI CCTC 9 cut(s) 102, 303, 340, 349, 493, 499, 533, 660, 779
Mph1103I ATGCAT 1 cut(s) 976
MseI TTAA 4 cut(s) 156, 207, 660, 1056
MspI CCGG 3 cut(s) 173, 185, 792
MspR9I CCNGG 1 cut(s) 106
MvaI CCWGG 1 cut(s) 106
MwoI GCNNNNNNNGC 4 cut(s) 695, 731, 986, 995
NarI GGCGCC 1 cut(s) 83
NcoI CCATGG 1 cut(s) 716
NdeII GATC 1 cut(s) 617
NlaIII CATG 2 cut(s) 584, 720
NlaIV GGNNCC 7 cut(s) 24, 84, 171, 183, 619, 745, 910
NmeAIII GCCGAG 1 cut(s) 530
NmuCI GTSAC 1 cut(s) 858
NsbI TGCGCA 1 cut(s) 1074
NsiI ATGCAT 1 cut(s) 976
NspV TTCGAA 1 cut(s) 41
PfeI GAWTC 2 cut(s) 222, 259
PflFI GACNNNGTC 1 cut(s) 1040
PfoI TCCNGGA 1 cut(s) 104
PkrI GCNGC 5 cut(s) 130, 169, 325, 328, 1077
PluTI GGCGCC 1 cut(s) 86
PshAI GACNNNNGTC 1 cut(s) 856
Psp6I CCWGG 1 cut(s) 104
PspGI CCWGG 1 cut(s) 104
PspN4I GGNNCC 7 cut(s) 24, 84, 171, 183, 619, 745, 910
PspPI GGNCC 1 cut(s) 395
PstI CTGCAG 1 cut(s) 133
PstNI CAGNNNCTG 1 cut(s) 137
PsuI RGATCY 1 cut(s) 617
PsyI GACNNNGTC 1 cut(s) 1040
RsaI GTAC 6 cut(s) 12, 67, 528, 632, 782, 1089
RsaNI GTAC 6 cut(s) 11, 66, 527, 631, 781, 1088
SaqAI TTAA 4 cut(s) 156, 207, 660, 1056
SatI GCNGC 5 cut(s) 129, 168, 324, 327, 1076
Sau3AI GATC 1 cut(s) 617
Sau96I GGNCC 1 cut(s) 395
ScrFI CCNGG 1 cut(s) 106
SduI GDGCHC 1 cut(s) 847
SfaNI GCATC 1 cut(s) 442
SfcI CTRYAG 1 cut(s) 129
SfoI GGCGCC 1 cut(s) 84
SfuI TTCGAA 1 cut(s) 41
SgrAI CRCCGGYG 1 cut(s) 184
SmlI CTYRAG 1 cut(s) 237
SmoI CTYRAG 1 cut(s) 237
Sse9I AATT 6 cut(s) 153, 337, 349, 477, 682, 762
SsiI CCGC 4 cut(s) 30, 167, 192, 318
SspDI GGCGCC 1 cut(s) 82
SspMI CTAG 3 cut(s) 33, 950, 983
StyD4I CCNGG 1 cut(s) 104
StyI CCWWGG 1 cut(s) 716
TaaI ACNGT 2 cut(s) 22, 121
TaqI TCGA 4 cut(s) 41, 200, 915, 932
TaqII GACCGA 1 cut(s) 456
TasI AATT 6 cut(s) 153, 337, 349, 477, 682, 762
TatI WGTACW 2 cut(s) 780, 1087
TauI GCSGC 1 cut(s) 170
TfiI GAWTC 2 cut(s) 222, 259
Tru1I TTAA 4 cut(s) 156, 207, 660, 1056
Tru9I TTAA 4 cut(s) 156, 207, 660, 1056
TscAI CASTG 3 cut(s) 412, 852, 1075
TseFI GTSAC 1 cut(s) 858
TseI GCWGC 4 cut(s) 128, 323, 326, 1075
Tsp45I GTSAC 1 cut(s) 858
TspDTI ATGAA 2 cut(s) 162, 332
TspGWI ACGGA 2 cut(s) 64, 82
TspRI CASTG 3 cut(s) 412, 852, 1075
Tth111I GACNNNGTC 1 cut(s) 1040
XapI RAATTY 3 cut(s) 337, 349, 682
XspI CTAG 3 cut(s) 33, 950, 983
Zsp2I ATGCAT 1 cut(s) 976
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.