FvH4_6g19500

Regulates membrane-cell wall junctions and localized cell wall deposition. Required for establishment of the Casparian strip membrane domain (CSD) and the subsequent formation of Casparian strips, a cell wall modification of the root endodermis that determines an apoplastic barrier between the intraorganismal apoplasm and the extraorganismal apoplasm and prevents lateral diffusion (By similarity)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002734
Physical Location & Seq
Forward (+)
13033089 .. 13034304
1216 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g19500.t1

Sequence Viewer

Length: 603 bp
ATGAAGAGTGGAGAAGCAACCACCATTGATGTTCCTGAGGCAAGTGGAGCAACCAAAGGAAAAGCACCTGTTTTGATGGTTCCTAGGAAAGAAAAGGGAGGAATGAAGAGAGGGCTTGCGATTATTGACTTGATTATAAGGATAGGTGCAATGGTGGCTGCTCTAGCTGCTGCAGCCACCATGGGAACTAGTGATCAGAACCTTCCTTTCTTCACTCAGTTCTTCCAGTTTGAAGCTAGCTATGATGACATGCCTAGTTTCGAGTTTTTCTTGATAGCAATGTCAATTGTAGCTGGCTACCTGGTGCTATCTCTCCCCTTCTCCATAGTAGCCATAGTTCGCCCTCATGCCGGTGGACCAAGGCTCTTGCTCCTCATCGTGGACATTGCGGCGCTTACTCTAGCCACTTCTGCTGCTGCGGCTGCAACATCCATAGTCTACTTAGCCCACAATGGCAATTCCAACTCGAACTGGCTCGCCATTTGCAACCAGTTTGGTGATTTCTGCCAGAAGGTCAGTGGGGCTGTGGTGGCATCCTTCATCACTGTTGTTTCCTTCATGTTCTTGATTCTGCTGTCTGCTTTGGCTCTCCGAAAGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

201

Amino Acids

21.13

Weight (kDa)

8.62

Isoelectric Point (pI)

19.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 37 - 184 1.3e-46 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 137
AccI GTMKAC 1 cut(s) 438
AciI CCGC 2 cut(s) 389, 419
AfiI CCNNNNNNNGG 2 cut(s) 350, 379
AgsI TTSAA 1 cut(s) 233
AhlI ACTAGT 1 cut(s) 188
AjnI CCWGG 1 cut(s) 300
AluBI AGCT 4 cut(s) 167, 236, 240, 293
AluI AGCT 4 cut(s) 167, 236, 240, 293
ApeKI GCWGC 7 cut(s) 158, 167, 170, 173, 413, 416, 422
AspA2I CCTAGG 1 cut(s) 83
AspLEI GCGC 1 cut(s) 394
AspS9I GGNCC 1 cut(s) 356
AsuHPI GGTGA 1 cut(s) 509
AsuNHI GCTAGC 1 cut(s) 236
AvaII GGWCC 1 cut(s) 356
AvrII CCTAGG 1 cut(s) 83
AxyI CCTNAGG 1 cut(s) 36
BbvI GCAGC 7 cut(s) 145, 154, 157, 185, 400, 403, 409
BccI CCATC 1 cut(s) 70
BciT130I CCWGG 1 cut(s) 302
BclI TGATCA 1 cut(s) 193
BcuI ACTAGT 1 cut(s) 188
BfaI CTAG 6 cut(s) 84, 164, 189, 237, 255, 401
BfmI CTRYAG 1 cut(s) 171
BfoI RGCGCY 1 cut(s) 395
BisI GCNGC 9 cut(s) 159, 168, 171, 174, 390, 414, 417, 420, 423
BlnI CCTAGG 1 cut(s) 83
BlsI GCNGC 9 cut(s) 160, 169, 172, 175, 391, 415, 418, 421, 424
Bme1390I CCNGG 1 cut(s) 302
Bme18I GGWCC 1 cut(s) 356
BmgT120I GGNCC 1 cut(s) 356
BmiI GGNNCC 1 cut(s) 81
BmrFI CCNGG 1 cut(s) 302
BmsI GCATC 1 cut(s) 542
BmtI GCTAGC 1 cut(s) 240
BsaJI CCNNGG 3 cut(s) 83, 180, 359
Bsc4I CCNNNNNNNGG 2 cut(s) 350, 379
Bse118I RCCGGY 1 cut(s) 350
Bse1I ACTGG 3 cut(s) 226, 476, 490
Bse21I CCTNAGG 1 cut(s) 36
Bse3DI GCAATG 3 cut(s) 156, 285, 384
BseBI CCWGG 1 cut(s) 302
BseDI CCNNGG 3 cut(s) 83, 180, 359
BseGI GGATG 2 cut(s) 428, 533
BseLI CCNNNNNNNGG 2 cut(s) 350, 379
BseMI GCAATG 3 cut(s) 156, 285, 384
BseMII CTCAG 2 cut(s) 27, 230
BseNI ACTGG 3 cut(s) 226, 476, 490
BseRI GAGGAG 1 cut(s) 362
BseXI GCAGC 7 cut(s) 145, 154, 157, 185, 400, 403, 409
BsiSI CCGG 1 cut(s) 351
BslI CCNNNNNNNGG 2 cut(s) 350, 379
Bsp143I GATC 1 cut(s) 193
Bsp19I CCATGG 1 cut(s) 180
BspACI CCGC 2 cut(s) 389, 419
BspCNI CTCAG 2 cut(s) 28, 229
BspLI GGNNCC 1 cut(s) 81
BspMAI CTGCAG 1 cut(s) 175
BspOI GCTAGC 1 cut(s) 240
BsrDI GCAATG 3 cut(s) 156, 285, 384
BsrFI RCCGGY 1 cut(s) 350
BsrI ACTGG 3 cut(s) 226, 476, 490
BssAI RCCGGY 1 cut(s) 350
BssECI CCNNGG 3 cut(s) 83, 180, 359
BssMI GATC 1 cut(s) 193
BssT1I CCWWGG 3 cut(s) 83, 180, 359
Bst2UI CCWGG 1 cut(s) 302
Bst4CI ACNGT 1 cut(s) 547
Bst6I CTCTTC 1 cut(s) 101
BstC8I GCNNGC 4 cut(s) 117, 238, 295, 477
BstDEI CTNAG 3 cut(s) 36, 216, 442
BstDSI CCRYGG 1 cut(s) 180
BstF5I GGATG 2 cut(s) 428, 533
BstH2I RGCGCY 1 cut(s) 395
BstHHI GCGC 1 cut(s) 394
BstKTI GATC 1 cut(s) 196
BstMBI GATC 1 cut(s) 193
BstMWI GCNNNNNNNGC 9 cut(s) 47, 155, 164, 167, 173, 410, 419, 422, 530
BstNI CCWGG 1 cut(s) 302
BstNSI RCATGY 1 cut(s) 253
BstSCI CCNGG 1 cut(s) 300
BstSFI CTRYAG 1 cut(s) 171
BstV1I GCAGC 7 cut(s) 145, 154, 157, 185, 400, 403, 409
Bsu36I CCTNAGG 1 cut(s) 36
BtgI CCRYGG 1 cut(s) 180
BtsCI GGATG 2 cut(s) 428, 533
BtsIMutI CAGTG 2 cut(s) 523, 543
Cac8I GCNNGC 4 cut(s) 117, 238, 295, 477
CfoI GCGC 1 cut(s) 394
Cfr10I RCCGGY 1 cut(s) 350
Cfr13I GGNCC 1 cut(s) 356
CsiI ACCWGGT 1 cut(s) 300
CviAII CATG 4 cut(s) 181, 250, 347, 559
DdeI CTNAG 3 cut(s) 36, 216, 442
DpnI GATC 1 cut(s) 195
DpnII GATC 1 cut(s) 193
Eam1104I CTCTTC 1 cut(s) 101
EarI CTCTTC 1 cut(s) 101
Eco130I CCWWGG 3 cut(s) 83, 180, 359
Eco47I GGWCC 1 cut(s) 356
Eco81I CCTNAGG 1 cut(s) 36
EcoRII CCWGG 1 cut(s) 300
EcoT14I CCWWGG 3 cut(s) 83, 180, 359
ErhI CCWWGG 3 cut(s) 83, 180, 359
FaeI CATG 4 cut(s) 184, 253, 350, 562
FaiI YATR 9 cut(s) 137, 182, 243, 251, 326, 335, 348, 434, 560
FatI CATG 4 cut(s) 180, 249, 346, 558
FbaI TGATCA 1 cut(s) 193
FblI GTMKAC 1 cut(s) 438
Fnu4HI GCNGC 9 cut(s) 159, 168, 171, 174, 390, 414, 417, 420, 423
FokI GGATG 2 cut(s) 415, 520
Fsp4HI GCNGC 9 cut(s) 159, 168, 171, 174, 390, 414, 417, 420, 423
FspBI CTAG 6 cut(s) 84, 164, 189, 237, 255, 401
GlaI GCGC 1 cut(s) 393
GluI GCNGC 9 cut(s) 159, 168, 171, 174, 390, 414, 417, 420, 423
HaeII RGCGCY 1 cut(s) 395
HapII CCGG 1 cut(s) 351
HhaI GCGC 1 cut(s) 394
Hin1II CATG 4 cut(s) 184, 253, 350, 562
Hin6I GCGC 1 cut(s) 392
HinP1I GCGC 1 cut(s) 392
HinfI GANTC 1 cut(s) 568
HpaII CCGG 1 cut(s) 351
HphI GGTGA 1 cut(s) 509
Hpy166II GTNNAC 3 cut(s) 356, 382, 439
Hpy188I TCNGA 2 cut(s) 198, 593
Hpy188III TCNNGA 3 cut(s) 35, 271, 565
Hpy8I GTNNAC 3 cut(s) 356, 382, 439
HpyAV CCTTC 5 cut(s) 212, 328, 505, 547, 565
HpyCH4III ACNGT 1 cut(s) 547
HpyCH4V TGCA 4 cut(s) 149, 173, 425, 486
HpyF10VI GCNNNNNNNGC 9 cut(s) 47, 155, 164, 167, 173, 410, 419, 422, 530
HpyF3I CTNAG 3 cut(s) 36, 216, 442
Hsp92II CATG 4 cut(s) 184, 253, 350, 562
HspAI GCGC 1 cut(s) 392
Ksp22I TGATCA 1 cut(s) 193
Kzo9I GATC 1 cut(s) 193
LmnI GCTCC 2 cut(s) 47, 375
Lsp1109I GCAGC 7 cut(s) 145, 154, 157, 185, 400, 403, 409
LweI GCATC 1 cut(s) 542
MabI ACCWGGT 1 cut(s) 300
MaeI CTAG 6 cut(s) 84, 164, 189, 237, 255, 401
MalI GATC 1 cut(s) 195
MboI GATC 1 cut(s) 193
MboII GAAGA 4 cut(s) 16, 118, 202, 214
MfeI CAATTG 1 cut(s) 285
MluCI AATT 2 cut(s) 285, 457
MmeI TCCRAC 1 cut(s) 486
MnlI CCTC 5 cut(s) 31, 92, 104, 354, 383
MseI TTAA 1 cut(s) 601
MslI CAYNNNNRTG 1 cut(s) 351
MspI CCGG 1 cut(s) 351
MspR9I CCNGG 1 cut(s) 302
MunI CAATTG 1 cut(s) 285
MvaI CCWGG 1 cut(s) 302
MwoI GCNNNNNNNGC 9 cut(s) 47, 155, 164, 167, 173, 410, 419, 422, 530
NcoI CCATGG 1 cut(s) 180
NdeII GATC 1 cut(s) 193
NheI GCTAGC 1 cut(s) 236
NlaIII CATG 4 cut(s) 184, 253, 350, 562
NlaIV GGNNCC 1 cut(s) 81
NspI RCATGY 1 cut(s) 253
PfeI GAWTC 1 cut(s) 568
PkrI GCNGC 9 cut(s) 160, 169, 172, 175, 391, 415, 418, 421, 424
PsiI TTATAA 1 cut(s) 137
Psp6I CCWGG 1 cut(s) 300
PspGI CCWGG 1 cut(s) 300
PspN4I GGNNCC 1 cut(s) 81
PspPI GGNCC 1 cut(s) 356
PsrI GAACNNNNNNTAC 2 cut(s) 321, 353
PstI CTGCAG 1 cut(s) 175
RseI CAYNNNNRTG 1 cut(s) 351
SaqAI TTAA 1 cut(s) 601
SatI GCNGC 9 cut(s) 159, 168, 171, 174, 390, 414, 417, 420, 423
Sau3AI GATC 1 cut(s) 193
Sau96I GGNCC 1 cut(s) 356
ScrFI CCNGG 1 cut(s) 302
SetI ASST 9 cut(s) 70, 148, 169, 204, 238, 242, 295, 303, 516
SexAI ACCWGGT 1 cut(s) 300
SfaNI GCATC 1 cut(s) 542
SfcI CTRYAG 1 cut(s) 171
SinI GGWCC 1 cut(s) 356
SmiMI CAYNNNNRTG 1 cut(s) 351
SpeI ACTAGT 1 cut(s) 188
Sse9I AATT 2 cut(s) 285, 457
SsiI CCGC 2 cut(s) 389, 419
SspMI CTAG 6 cut(s) 84, 164, 189, 237, 255, 401
StyD4I CCNGG 1 cut(s) 300
StyI CCWWGG 3 cut(s) 83, 180, 359
TaaI ACNGT 1 cut(s) 547
TaqI TCGA 2 cut(s) 261, 467
TasI AATT 2 cut(s) 285, 457
TauI GCSGC 2 cut(s) 392, 422
TfiI GAWTC 1 cut(s) 568
Tru1I TTAA 1 cut(s) 601
Tru9I TTAA 1 cut(s) 601
TscAI CASTG 2 cut(s) 523, 550
TseI GCWGC 7 cut(s) 158, 167, 170, 173, 413, 416, 422
TspDTI ATGAA 4 cut(s) 17, 119, 529, 547
TspRI CASTG 2 cut(s) 523, 550
VpaK11BI GGWCC 1 cut(s) 356
XceI RCATGY 1 cut(s) 253
XcmI CCANNNNNNNNNTGG 1 cut(s) 515
XmaJI CCTAGG 1 cut(s) 83
XmiI GTMKAC 1 cut(s) 438
XspI CTAG 6 cut(s) 84, 164, 189, 237, 255, 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.