MD12G1031200.v1.1

Regulates membrane-cell wall junctions and localized cell wall deposition. Required for establishment of the Casparian strip membrane domain (CSD) and the subsequent formation of Casparian strips, a cell wall modification of the root endodermis that determines an apoplastic barrier between the intraorganismal apoplasm and the extraorganismal apoplasm and prevents lateral diffusion (By similarity)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002734
Physical Location & Seq
Forward (+)
3451674 .. 3452776
1103 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1031200.v1.1.491

Sequence Viewer

Length: 618 bp
ATGAAGAGCAGCGGCGACTCGACCACCATTGATGTTGTTGCTCACGAGGGAAGTGGTACATCCAAAGGGAAAGGGCCCATTCTGATGGTTCCTGTAAAGGAAGGAAAAGGTGGAATGAAGAGAGGGATGGCCATAATTGACTTGGTTTTGAGGAGTGGTGCTATAGTGTCTGCTCTTGCTGCTGCTGCGACCATGGGAACCAGTGATCAAAACCTTCCCTTCTTCACCCAGTTCTTCCAGTTTGAAGCTAGCTATGATGACATGCCTAGTTTTCAGTTTTTCCTGATAGCAATGTCACTTGTAGCGGGATACTTGGTGCTCTCAATTCCCTTTGCCATAGTCTCCATTGTTCGCCCCCATGCAAGTGGACCAAGGCTCTTGCTCCTCATCCTTGACGTTGTGGCACTGACTCTAGCCACTTCTGCTGCTGGGGCTGCAACATCCATAGTCTACCTAGCCCACAATGGCAATGCAAGCTCCAACTGGCTAGCCATCTGCAACCAGTTTGGTGATTTCTGCCGGAACGTGAGTGGGGCTGTGGTGGCTTCCTTCGTTACTGTCGTCACCTTCATGTTCTTGATTCTGCTCTCTGCTTTGGCTCTCCGAAAGCATCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

206

Amino Acids

21.56

Weight (kDa)

8.64

Isoelectric Point (pI)

23.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 41 - 188 2.1e-45 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 450
AciI CCGC 2 cut(s) 12, 305
AcoI YGGCCR 1 cut(s) 129
AfaI GTAC 1 cut(s) 58
AgsI TTSAA 1 cut(s) 245
AluBI AGCT 3 cut(s) 248, 252, 477
AluI AGCT 3 cut(s) 248, 252, 477
Alw21I GWGCWC 1 cut(s) 321
Alw26I GTCTC 1 cut(s) 346
AoxI GGCC 2 cut(s) 74, 129
ApaI GGGCCC 1 cut(s) 78
ApeKI GCWGC 6 cut(s) 9, 179, 182, 185, 425, 434
AspS9I GGNCC 3 cut(s) 74, 75, 368
AsuHPI GGTGA 3 cut(s) 217, 521, 556
AsuNHI GCTAGC 2 cut(s) 248, 487
AvaII GGWCC 1 cut(s) 368
BaeGI GKGCMC 1 cut(s) 78
BalI TGGCCA 1 cut(s) 131
BanII GRGCYC 1 cut(s) 78
BauI CACGAG 1 cut(s) 44
Bbv12I GWGCWC 1 cut(s) 321
BbvI GCAGC 6 cut(s) 21, 166, 169, 172, 412, 421
BccI CCATC 3 cut(s) 79, 121, 500
BciVI GTATCC 1 cut(s) 302
BclI TGATCA 1 cut(s) 205
BcoDI GTCTC 1 cut(s) 346
BfaI CTAG 5 cut(s) 249, 267, 413, 455, 488
BfmI CTRYAG 1 cut(s) 162
BfuI GTATCC 1 cut(s) 302
BisI GCNGC 7 cut(s) 10, 13, 180, 183, 186, 426, 435
BlsI GCNGC 7 cut(s) 11, 14, 181, 184, 187, 427, 436
Bme18I GGWCC 1 cut(s) 368
BmgT120I GGNCC 3 cut(s) 74, 75, 368
BmiI GGNNCC 3 cut(s) 76, 90, 199
BmrI ACTGGG 1 cut(s) 223
BmtI GCTAGC 2 cut(s) 252, 491
BmuI ACTGGG 1 cut(s) 223
BsaJI CCNNGG 2 cut(s) 192, 371
Bse1I ACTGG 5 cut(s) 201, 229, 238, 488, 502
Bse3DI GCAATG 2 cut(s) 297, 475
BseDI CCNNGG 2 cut(s) 192, 371
BseGI GGATG 4 cut(s) 59, 132, 387, 440
BseMI GCAATG 2 cut(s) 297, 475
BseNI ACTGG 5 cut(s) 201, 229, 238, 488, 502
BseRI GAGGAG 2 cut(s) 166, 374
BseSI GKGCMC 1 cut(s) 78
BseXI GCAGC 6 cut(s) 21, 166, 169, 172, 412, 421
BseYI CCCAGC 1 cut(s) 428
BshFI GGCC 2 cut(s) 76, 131
BsiHKAI GWGCWC 1 cut(s) 321
BsiSI CCGG 1 cut(s) 520
BsmAI GTCTC 1 cut(s) 346
BsnI GGCC 2 cut(s) 76, 131
Bsp120I GGGCCC 1 cut(s) 74
Bsp1286I GDGCHC 2 cut(s) 78, 321
Bsp143I GATC 1 cut(s) 205
Bsp19I CCATGG 1 cut(s) 192
BspACI CCGC 2 cut(s) 12, 305
BspANI GGCC 2 cut(s) 76, 131
BspLI GGNNCC 3 cut(s) 76, 90, 199
BspOI GCTAGC 2 cut(s) 252, 491
BsrDI GCAATG 2 cut(s) 297, 475
BsrI ACTGG 5 cut(s) 201, 229, 238, 488, 502
BssECI CCNNGG 2 cut(s) 192, 371
BssMI GATC 1 cut(s) 205
BssSI CACGAG 1 cut(s) 44
BssT1I CCWWGG 2 cut(s) 192, 371
Bst2BI CACGAG 1 cut(s) 44
Bst4CI ACNGT 1 cut(s) 559
Bst6I CTCTTC 1 cut(s) 113
BstC8I GCNNGC 3 cut(s) 250, 475, 489
BstDSI CCRYGG 1 cut(s) 192
BstF5I GGATG 4 cut(s) 59, 132, 387, 440
BstKTI GATC 1 cut(s) 208
BstMAI GTCTC 1 cut(s) 346
BstMBI GATC 1 cut(s) 205
BstMWI GCNNNNNNNGC 7 cut(s) 179, 185, 422, 431, 434, 474, 542
BstNSI RCATGY 1 cut(s) 265
BstSFI CTRYAG 1 cut(s) 162
BstSLI GKGCMC 1 cut(s) 78
BstV1I GCAGC 6 cut(s) 21, 166, 169, 172, 412, 421
BstXI CCANNNNNNTGG 2 cut(s) 85, 365
BsuI GTATCC 1 cut(s) 302
BsuRI GGCC 2 cut(s) 76, 131
BtgI CCRYGG 1 cut(s) 192
BtsCI GGATG 4 cut(s) 59, 132, 387, 440
BtsIMutI CAGTG 3 cut(s) 208, 404, 613
Cac8I GCNNGC 3 cut(s) 250, 475, 489
Cfr13I GGNCC 3 cut(s) 74, 75, 368
Csp6I GTAC 1 cut(s) 57
CviAII CATG 4 cut(s) 193, 262, 359, 571
CviQI GTAC 1 cut(s) 57
DpnI GATC 1 cut(s) 207
DpnII GATC 1 cut(s) 205
EaeI YGGCCR 1 cut(s) 129
Eam1104I CTCTTC 1 cut(s) 113
EarI CTCTTC 1 cut(s) 113
Eco130I CCWWGG 2 cut(s) 192, 371
Eco24I GRGCYC 1 cut(s) 78
Eco47I GGWCC 1 cut(s) 368
EcoO109I RGGNCCY 1 cut(s) 74
EcoT14I CCWWGG 2 cut(s) 192, 371
EcoT38I GRGCYC 1 cut(s) 78
ErhI CCWWGG 2 cut(s) 192, 371
FaeI CATG 4 cut(s) 196, 265, 362, 574
FaiI YATR 9 cut(s) 134, 164, 194, 255, 263, 338, 360, 446, 572
FatI CATG 4 cut(s) 192, 261, 358, 570
FauI CCCGC 1 cut(s) 298
FbaI TGATCA 1 cut(s) 205
FblI GTMKAC 1 cut(s) 450
Fnu4HI GCNGC 7 cut(s) 10, 13, 180, 183, 186, 426, 435
FokI GGATG 4 cut(s) 46, 139, 374, 427
FriOI GRGCYC 1 cut(s) 78
Fsp4HI GCNGC 7 cut(s) 10, 13, 180, 183, 186, 426, 435
FspBI CTAG 5 cut(s) 249, 267, 413, 455, 488
GluI GCNGC 7 cut(s) 10, 13, 180, 183, 186, 426, 435
GsaI CCCAGC 1 cut(s) 432
HaeIII GGCC 2 cut(s) 76, 131
HapII CCGG 1 cut(s) 520
Hin1II CATG 4 cut(s) 196, 265, 362, 574
HinfI GANTC 3 cut(s) 17, 409, 580
HpaII CCGG 1 cut(s) 520
HphI GGTGA 3 cut(s) 217, 521, 556
Hpy166II GTNNAC 2 cut(s) 368, 451
Hpy188I TCNGA 2 cut(s) 84, 605
Hpy188III TCNNGA 3 cut(s) 44, 283, 577
Hpy8I GTNNAC 2 cut(s) 368, 451
HpyAV CCTTC 5 cut(s) 95, 224, 229, 559, 577
HpyCH4III ACNGT 1 cut(s) 559
HpyCH4IV ACGT 2 cut(s) 396, 525
HpyCH4V TGCA 4 cut(s) 362, 437, 473, 498
HpyF10VI GCNNNNNNNGC 7 cut(s) 179, 185, 422, 431, 434, 474, 542
HpySE526I ACGT 2 cut(s) 396, 525
Hsp92II CATG 4 cut(s) 196, 265, 362, 574
Ksp22I TGATCA 1 cut(s) 205
Kzo9I GATC 1 cut(s) 205
LmnI GCTCC 2 cut(s) 387, 482
LpnPI CCDG 9 cut(s) 105, 214, 242, 251, 296, 414, 469, 515, 533
Lsp1109I GCAGC 6 cut(s) 21, 166, 169, 172, 412, 421
MaeI CTAG 5 cut(s) 249, 267, 413, 455, 488
MaeII ACGT 2 cut(s) 396, 525
MaeIII GTNAC 3 cut(s) 294, 553, 562
MalI GATC 1 cut(s) 207
MboI GATC 1 cut(s) 205
MboII GAAGA 4 cut(s) 16, 130, 214, 226
MhlI GDGCHC 2 cut(s) 78, 321
MlsI TGGCCA 1 cut(s) 131
MluCI AATT 2 cut(s) 135, 324
MluNI TGGCCA 1 cut(s) 131
MlyI GAGTC 2 cut(s) 11, 403
MmeI TCCRAC 1 cut(s) 504
MnlI CCTC 4 cut(s) 40, 116, 144, 395
Mox20I TGGCCA 1 cut(s) 131
MscI TGGCCA 1 cut(s) 131
MslI CAYNNNNRTG 3 cut(s) 83, 363, 569
Msp20I TGGCCA 1 cut(s) 131
MspA1I CMGCKG 1 cut(s) 12
MspI CCGG 1 cut(s) 520
MwoI GCNNNNNNNGC 7 cut(s) 179, 185, 422, 431, 434, 474, 542
NcoI CCATGG 1 cut(s) 192
NdeII GATC 1 cut(s) 205
NheI GCTAGC 2 cut(s) 248, 487
NlaIII CATG 4 cut(s) 196, 265, 362, 574
NlaIV GGNNCC 3 cut(s) 76, 90, 199
NmuCI GTSAC 2 cut(s) 294, 562
NspI RCATGY 1 cut(s) 265
PcsI WCGNNNNNNNCGW 1 cut(s) 558
PfeI GAWTC 1 cut(s) 580
PkrI GCNGC 7 cut(s) 11, 14, 181, 184, 187, 427, 436
PleI GAGTC 2 cut(s) 11, 403
PpsI GAGTC 2 cut(s) 11, 403
PspFI CCCAGC 1 cut(s) 428
PspN4I GGNNCC 3 cut(s) 76, 90, 199
PspOMI GGGCCC 1 cut(s) 74
PspPI GGNCC 3 cut(s) 74, 75, 368
RsaI GTAC 1 cut(s) 58
RsaNI GTAC 1 cut(s) 57
RseI CAYNNNNRTG 3 cut(s) 83, 363, 569
SatI GCNGC 7 cut(s) 10, 13, 180, 183, 186, 426, 435
Sau3AI GATC 1 cut(s) 205
Sau96I GGNCC 3 cut(s) 74, 75, 368
SchI GAGTC 2 cut(s) 11, 403
SduI GDGCHC 2 cut(s) 78, 321
SetI ASST 9 cut(s) 112, 216, 250, 254, 399, 456, 479, 528, 569
SfcI CTRYAG 1 cut(s) 162
SinI GGWCC 1 cut(s) 368
SmiMI CAYNNNNRTG 3 cut(s) 83, 363, 569
Sse9I AATT 2 cut(s) 135, 324
SsiI CCGC 2 cut(s) 12, 305
SspMI CTAG 5 cut(s) 249, 267, 413, 455, 488
StyI CCWWGG 2 cut(s) 192, 371
TaaI ACNGT 1 cut(s) 559
TaiI ACGT 2 cut(s) 399, 528
TaqI TCGA 1 cut(s) 20
TasI AATT 2 cut(s) 135, 324
TauI GCSGC 1 cut(s) 15
TfiI GAWTC 1 cut(s) 580
TscAI CASTG 2 cut(s) 208, 411
TseFI GTSAC 2 cut(s) 294, 562
TseI GCWGC 6 cut(s) 9, 179, 182, 185, 425, 434
Tsp45I GTSAC 2 cut(s) 294, 562
TspDTI ATGAA 3 cut(s) 17, 131, 559
TspRI CASTG 2 cut(s) 208, 411
VpaK11BI GGWCC 1 cut(s) 368
XceI RCATGY 1 cut(s) 265
XcmI CCANNNNNNNNNTGG 1 cut(s) 139
XmiI GTMKAC 1 cut(s) 450
XspI CTAG 5 cut(s) 249, 267, 413, 455, 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.