MD12G1031100.v1.1

Regulates membrane-cell wall junctions and localized cell wall deposition. Required for establishment of the Casparian strip membrane domain (CSD) and the subsequent formation of Casparian strips, a cell wall modification of the root endodermis that determines an apoplastic barrier between the intraorganismal apoplasm and the extraorganismal apoplasm and prevents lateral diffusion (By similarity)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002734
Physical Location & Seq
Forward (+)
3442543 .. 3443655
1113 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1031100.v1.1.491

Sequence Viewer

Length: 612 bp
ATGAAGAGTGGCGGCGACTCGACCACCATTGATGTTGAGGCAAGAAGTCCTTCCAAAGGAAAAGGGCCTGTTCTGGCAGTTCATCATGTGAAGGAAGAAAAGGGAGGAATGAAGAGAGGGATGGCCATAATCGACTTGGTATTGAGGATTGGTGCTATAGTGGCTGCTCTTGCTGCTGCTGCCACCATGGGAACCAGTGATCAAAACCTTCCTTTCTTCACCCAGTTCTTCCAATTTGAAGCTAGCTATGATGACATGCCTAGCTTTCAGTTTTTCCTAATAGCAATGTCAATTGTAGCTGGCTACTTGGTGCTCTCACTTCCCTTCTCCATCGTCTCCATCGTTCGCCCCCATGCAAGTGGACCAAGGCTCTTGCTCCTCATCCTTGACCTTGTGGCACTGACTCTAGCCACTTCTGCTGCTGGGGCTGCAACATCCATAGTCTACCTAGCCCACAATGGCAATGCAAGCTCCAACTGGCTAGCCATCTGCAACCAGTTTGGTGATTTCTGCCGGAACGTGAGTGGGGCTGTGGTGGCTTCCTTCGTTACTGTCGTCACCTTCATGTTCTTGATTCTGCTCTCTGCTTTGGCTCTCCGAAAGCATCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

204

Amino Acids

21.55

Weight (kDa)

8.64

Isoelectric Point (pI)

28.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 39 - 186 2.3e-47 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 444
AciI CCGC 1 cut(s) 12
AcoI YGGCCR 1 cut(s) 123
AfiI CCNNNNNNNGG 1 cut(s) 56
AgsI TTSAA 1 cut(s) 239
AluBI AGCT 5 cut(s) 242, 246, 264, 299, 471
AluI AGCT 5 cut(s) 242, 246, 264, 299, 471
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 1 cut(s) 340
AoxI GGCC 2 cut(s) 65, 123
ApeKI GCWGC 6 cut(s) 164, 173, 176, 179, 419, 428
Asp700I GAANNNNTTC 1 cut(s) 49
AspS9I GGNCC 2 cut(s) 65, 362
AsuHPI GGTGA 3 cut(s) 211, 515, 550
AsuNHI GCTAGC 2 cut(s) 242, 481
AvaII GGWCC 1 cut(s) 362
BalI TGGCCA 1 cut(s) 125
Bbv12I GWGCWC 1 cut(s) 315
BbvI GCAGC 6 cut(s) 151, 160, 163, 166, 406, 415
BccI CCATC 4 cut(s) 115, 338, 347, 494
BclI TGATCA 1 cut(s) 199
BcoDI GTCTC 1 cut(s) 340
BfaI CTAG 5 cut(s) 243, 261, 407, 449, 482
BfmI CTRYAG 1 cut(s) 156
BisI GCNGC 7 cut(s) 13, 165, 174, 177, 180, 420, 429
BlsI GCNGC 7 cut(s) 14, 166, 175, 178, 181, 421, 430
Bme18I GGWCC 1 cut(s) 362
BmgT120I GGNCC 2 cut(s) 65, 362
BmiI GGNNCC 1 cut(s) 193
BmrI ACTGGG 1 cut(s) 217
BmtI GCTAGC 2 cut(s) 246, 485
BmuI ACTGGG 1 cut(s) 217
BsaJI CCNNGG 2 cut(s) 186, 365
Bsc4I CCNNNNNNNGG 1 cut(s) 56
Bse1I ACTGG 4 cut(s) 195, 223, 482, 496
Bse3DI GCAATG 2 cut(s) 291, 469
BseDI CCNNGG 2 cut(s) 186, 365
BseGI GGATG 3 cut(s) 126, 381, 434
BseLI CCNNNNNNNGG 1 cut(s) 56
BseMI GCAATG 2 cut(s) 291, 469
BseNI ACTGG 4 cut(s) 195, 223, 482, 496
BseRI GAGGAG 1 cut(s) 368
BseXI GCAGC 6 cut(s) 151, 160, 163, 166, 406, 415
BseYI CCCAGC 1 cut(s) 422
BshFI GGCC 2 cut(s) 67, 125
BsiHKAI GWGCWC 1 cut(s) 315
BsiSI CCGG 1 cut(s) 514
BslI CCNNNNNNNGG 1 cut(s) 56
BsmAI GTCTC 1 cut(s) 340
BsmBI CGTCTC 1 cut(s) 340
BsnI GGCC 2 cut(s) 67, 125
Bsp1286I GDGCHC 1 cut(s) 315
Bsp143I GATC 1 cut(s) 199
Bsp19I CCATGG 1 cut(s) 186
BspACI CCGC 1 cut(s) 12
BspANI GGCC 2 cut(s) 67, 125
BspLI GGNNCC 1 cut(s) 193
BspOI GCTAGC 2 cut(s) 246, 485
BsrDI GCAATG 2 cut(s) 291, 469
BsrI ACTGG 4 cut(s) 195, 223, 482, 496
BssECI CCNNGG 2 cut(s) 186, 365
BssMI GATC 1 cut(s) 199
BssT1I CCWWGG 2 cut(s) 186, 365
Bst4CI ACNGT 1 cut(s) 553
Bst6I CTCTTC 1 cut(s) 107
BstC8I GCNNGC 4 cut(s) 244, 301, 469, 483
BstDSI CCRYGG 1 cut(s) 186
BstENI CCTNNNNNAGG 1 cut(s) 54
BstF5I GGATG 3 cut(s) 126, 381, 434
BstKTI GATC 1 cut(s) 202
BstMAI GTCTC 1 cut(s) 340
BstMBI GATC 1 cut(s) 199
BstMWI GCNNNNNNNGC 9 cut(s) 161, 170, 173, 179, 416, 425, 428, 468, 536
BstNSI RCATGY 1 cut(s) 259
BstSFI CTRYAG 1 cut(s) 156
BstV1I GCAGC 6 cut(s) 151, 160, 163, 166, 406, 415
BstXI CCANNNNNNTGG 1 cut(s) 359
BsuRI GGCC 2 cut(s) 67, 125
BtgI CCRYGG 1 cut(s) 186
BtsCI GGATG 3 cut(s) 126, 381, 434
BtsIMutI CAGTG 3 cut(s) 202, 398, 607
Cac8I GCNNGC 4 cut(s) 244, 301, 469, 483
Cfr13I GGNCC 2 cut(s) 65, 362
CviAII CATG 5 cut(s) 86, 187, 256, 353, 565
DpnI GATC 1 cut(s) 201
DpnII GATC 1 cut(s) 199
EaeI YGGCCR 1 cut(s) 123
Eam1104I CTCTTC 1 cut(s) 107
EarI CTCTTC 1 cut(s) 107
Eco130I CCWWGG 2 cut(s) 186, 365
Eco47I GGWCC 1 cut(s) 362
EcoNI CCTNNNNNAGG 1 cut(s) 54
EcoO109I RGGNCCY 1 cut(s) 65
EcoT14I CCWWGG 2 cut(s) 186, 365
ErhI CCWWGG 2 cut(s) 186, 365
Esp3I CGTCTC 1 cut(s) 340
FaeI CATG 5 cut(s) 89, 190, 259, 356, 568
FaiI YATR 9 cut(s) 87, 128, 158, 188, 249, 257, 354, 440, 566
FalI AAGNNNNNCTT 2 cut(s) 34, 66
FatI CATG 5 cut(s) 85, 186, 255, 352, 564
FbaI TGATCA 1 cut(s) 199
FblI GTMKAC 1 cut(s) 444
Fnu4HI GCNGC 7 cut(s) 13, 165, 174, 177, 180, 420, 429
FokI GGATG 3 cut(s) 133, 368, 421
Fsp4HI GCNGC 7 cut(s) 13, 165, 174, 177, 180, 420, 429
FspBI CTAG 5 cut(s) 243, 261, 407, 449, 482
GluI GCNGC 7 cut(s) 13, 165, 174, 177, 180, 420, 429
GsaI CCCAGC 1 cut(s) 426
HaeIII GGCC 2 cut(s) 67, 125
HapII CCGG 1 cut(s) 514
Hin1II CATG 5 cut(s) 89, 190, 259, 356, 568
HinfI GANTC 3 cut(s) 17, 403, 574
HpaII CCGG 1 cut(s) 514
HphI GGTGA 3 cut(s) 211, 515, 550
Hpy166II GTNNAC 2 cut(s) 362, 445
Hpy188I TCNGA 1 cut(s) 599
Hpy188III TCNNGA 1 cut(s) 571
Hpy8I GTNNAC 2 cut(s) 362, 445
HpyAV CCTTC 6 cut(s) 60, 85, 218, 334, 553, 571
HpyCH4III ACNGT 1 cut(s) 553
HpyCH4IV ACGT 1 cut(s) 519
HpyCH4V TGCA 4 cut(s) 356, 431, 467, 492
HpyF10VI GCNNNNNNNGC 9 cut(s) 161, 170, 173, 179, 416, 425, 428, 468, 536
HpySE526I ACGT 1 cut(s) 519
Hsp92II CATG 5 cut(s) 89, 190, 259, 356, 568
Ksp22I TGATCA 1 cut(s) 199
Kzo9I GATC 1 cut(s) 199
LmnI GCTCC 2 cut(s) 381, 476
LpnPI CCDG 9 cut(s) 59, 81, 208, 236, 285, 408, 463, 509, 527
Lsp1109I GCAGC 6 cut(s) 151, 160, 163, 166, 406, 415
MaeI CTAG 5 cut(s) 243, 261, 407, 449, 482
MaeII ACGT 1 cut(s) 519
MaeIII GTNAC 2 cut(s) 547, 556
MalI GATC 1 cut(s) 201
MboI GATC 1 cut(s) 199
MboII GAAGA 5 cut(s) 16, 107, 124, 208, 220
MfeI CAATTG 1 cut(s) 291
MhlI GDGCHC 1 cut(s) 315
MlsI TGGCCA 1 cut(s) 125
MluCI AATT 2 cut(s) 233, 291
MluNI TGGCCA 1 cut(s) 125
MlyI GAGTC 2 cut(s) 11, 397
MmeI TCCRAC 1 cut(s) 498
MnlI CCTC 5 cut(s) 31, 98, 110, 138, 389
Mox20I TGGCCA 1 cut(s) 125
MroXI GAANNNNTTC 1 cut(s) 49
MscI TGGCCA 1 cut(s) 125
MslI CAYNNNNRTG 2 cut(s) 357, 563
Msp20I TGGCCA 1 cut(s) 125
MspI CCGG 1 cut(s) 514
MunI CAATTG 1 cut(s) 291
MwoI GCNNNNNNNGC 9 cut(s) 161, 170, 173, 179, 416, 425, 428, 468, 536
NcoI CCATGG 1 cut(s) 186
NdeII GATC 1 cut(s) 199
NheI GCTAGC 2 cut(s) 242, 481
NlaIII CATG 5 cut(s) 89, 190, 259, 356, 568
NlaIV GGNNCC 1 cut(s) 193
NmuCI GTSAC 1 cut(s) 556
NspI RCATGY 1 cut(s) 259
PcsI WCGNNNNNNNCGW 2 cut(s) 339, 552
PdmI GAANNNNTTC 1 cut(s) 49
PfeI GAWTC 1 cut(s) 574
PkrI GCNGC 7 cut(s) 14, 166, 175, 178, 181, 421, 430
PleI GAGTC 2 cut(s) 11, 397
PpsI GAGTC 2 cut(s) 11, 397
PspFI CCCAGC 1 cut(s) 422
PspN4I GGNNCC 1 cut(s) 193
PspPI GGNCC 2 cut(s) 65, 362
RseI CAYNNNNRTG 2 cut(s) 357, 563
SatI GCNGC 7 cut(s) 13, 165, 174, 177, 180, 420, 429
Sau3AI GATC 1 cut(s) 199
Sau96I GGNCC 2 cut(s) 65, 362
SchI GAGTC 2 cut(s) 11, 397
SduI GDGCHC 1 cut(s) 315
SfcI CTRYAG 1 cut(s) 156
SinI GGWCC 1 cut(s) 362
SmiMI CAYNNNNRTG 2 cut(s) 357, 563
Sse9I AATT 2 cut(s) 233, 291
SsiI CCGC 1 cut(s) 12
SspMI CTAG 5 cut(s) 243, 261, 407, 449, 482
StyI CCWWGG 2 cut(s) 186, 365
TaaI ACNGT 1 cut(s) 553
TaiI ACGT 1 cut(s) 522
TaqI TCGA 2 cut(s) 20, 132
TasI AATT 2 cut(s) 233, 291
TauI GCSGC 1 cut(s) 15
TfiI GAWTC 1 cut(s) 574
TscAI CASTG 2 cut(s) 202, 405
TseFI GTSAC 1 cut(s) 556
TseI GCWGC 6 cut(s) 164, 173, 176, 179, 419, 428
Tsp45I GTSAC 1 cut(s) 556
TspDTI ATGAA 4 cut(s) 17, 71, 125, 553
TspRI CASTG 2 cut(s) 202, 405
VpaK11BI GGWCC 1 cut(s) 362
XagI CCTNNNNNAGG 1 cut(s) 54
XceI RCATGY 1 cut(s) 259
XcmI CCANNNNNNNNNTGG 1 cut(s) 133
XmiI GTMKAC 1 cut(s) 444
XmnI GAANNNNTTC 1 cut(s) 49
XspI CTAG 5 cut(s) 243, 261, 407, 449, 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.