MD14G1031600.v1.1

Regulates membrane-cell wall junctions and localized cell wall deposition. Required for establishment of the Casparian strip membrane domain (CSD) and the subsequent formation of Casparian strips, a cell wall modification of the root endodermis that determines an apoplastic barrier between the intraorganismal apoplasm and the extraorganismal apoplasm and prevents lateral diffusion (By similarity)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0002734
Physical Location & Seq
Forward (+)
2814412 .. 2815695
1284 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1031600.v1.1.491

Sequence Viewer

Length: 735 bp
ATGTTTAACCATTTTCATCGCAAGCTTTTGAGTCTCTTCTCCTCAGCAATTCCCATTTCCAAAGCTTTAGTTTTCTGGTTTATCTCCTCTCTCGTTATCTCTCTCTTTCGGACGAAAATGAAGAGCGGAGGCGGCGGCGAGTCAACCACCATTGATATTGAGGCAAGAAGTGCTTCCAAAGGAAAAGGGCCTGTTATGGCAGTTCATCATGTGAAGAAAGAAAAGGGAGGAATGGAGAGAGGGATGGCCATAATTGACTTGGTTTTGAGGATTGGGGCAATAGTGGCTGCTCTTGCTGCTGCTGCCACCATGGGAACCAGTGATCAAAACCTTCCTTTCTTCACCCAGTTCTTCCAGTTTGAAGCTAGCTATGATGACATGCCTAGCTTTCAGTTTTTCCTGATAGCAATGTCACTTGTAGCTGGCTACTTGGTGCTCTCACTTCCCTTCTCCGTCGTCTGCATCGTTCGCCCCCATGCAAGTGGACCAAGGCTCTTGCTCCTCATCCTTGACCTTGTGGCACTGACTCTAGCCACTTCTGCTGCTGGGGCTGCAACATCCATAGTCTACTTAGCCCACAACGGCAACTCAAGCTCCAACTGGCTGGCCATCTGCAACCAGTTTGGTGATTTCTGCAGGAACGTGAGTGGGGCTGTGGTGGCTTCTTTCGTTACTGTTGTCACCTTCATGTTCTTGATTCTGCTGTCTGCGTTGGCTCTACGAAAGCATCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

245

Amino Acids

26.26

Weight (kDa)

9.58

Isoelectric Point (pI)

26.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 80 - 227 3.3e-47 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 126
AccI GTMKAC 1 cut(s) 567
AciI CCGC 3 cut(s) 126, 132, 135
AcoI YGGCCR 2 cut(s) 246, 606
AgsI TTSAA 1 cut(s) 362
AluBI AGCT 7 cut(s) 25, 65, 365, 369, 387, 422, 594
AluI AGCT 7 cut(s) 25, 65, 365, 369, 387, 422, 594
Alw21I GWGCWC 1 cut(s) 438
Alw26I GTCTC 1 cut(s) 38
AoxI GGCC 3 cut(s) 188, 246, 606
ApeKI GCWGC 6 cut(s) 287, 296, 299, 302, 542, 551
Asp700I GAANNNNTTC 1 cut(s) 172
AspS9I GGNCC 2 cut(s) 188, 485
AsuHPI GGTGA 3 cut(s) 334, 638, 673
AsuNHI GCTAGC 1 cut(s) 365
AvaII GGWCC 1 cut(s) 485
BalI TGGCCA 2 cut(s) 248, 608
Bbv12I GWGCWC 1 cut(s) 438
BbvCI CCTCAGC 1 cut(s) 43
BbvI GCAGC 6 cut(s) 274, 283, 286, 289, 529, 538
BccI CCATC 2 cut(s) 238, 617
BceAI ACGGC 1 cut(s) 598
BclI TGATCA 1 cut(s) 322
BcoDI GTCTC 1 cut(s) 38
BfaI CTAG 3 cut(s) 366, 384, 530
BfmI CTRYAG 1 cut(s) 634
BisI GCNGC 8 cut(s) 133, 136, 288, 297, 300, 303, 543, 552
BlsI GCNGC 8 cut(s) 134, 137, 289, 298, 301, 304, 544, 553
Bme18I GGWCC 1 cut(s) 485
BmgT120I GGNCC 2 cut(s) 188, 485
BmiI GGNNCC 1 cut(s) 316
BmrI ACTGGG 1 cut(s) 340
BmsI GCATC 1 cut(s) 471
BmtI GCTAGC 1 cut(s) 369
BmuI ACTGGG 1 cut(s) 340
Bpu10I CCTNAGC 1 cut(s) 43
BpuEI CTTGAG 1 cut(s) 574
BsaJI CCNNGG 2 cut(s) 309, 488
BsaXI ACNNNNNCTCC 2 cut(s) 578, 608
Bse1I ACTGG 5 cut(s) 318, 346, 355, 605, 619
Bse3DI GCAATG 1 cut(s) 414
BseDI CCNNGG 2 cut(s) 309, 488
BseGI GGATG 3 cut(s) 249, 504, 557
BseMI GCAATG 1 cut(s) 414
BseMII CTCAG 1 cut(s) 57
BseNI ACTGG 5 cut(s) 318, 346, 355, 605, 619
BseRI GAGGAG 3 cut(s) 31, 76, 491
BseXI GCAGC 6 cut(s) 274, 283, 286, 289, 529, 538
BseYI CCCAGC 1 cut(s) 545
BshFI GGCC 3 cut(s) 190, 248, 608
BsiHKAI GWGCWC 1 cut(s) 438
BsmAI GTCTC 1 cut(s) 38
BsnI GGCC 3 cut(s) 190, 248, 608
Bsp1286I GDGCHC 1 cut(s) 438
Bsp143I GATC 1 cut(s) 322
Bsp19I CCATGG 1 cut(s) 309
BspACI CCGC 3 cut(s) 126, 132, 135
BspANI GGCC 3 cut(s) 190, 248, 608
BspCNI CTCAG 1 cut(s) 56
BspLI GGNNCC 1 cut(s) 316
BspMAI CTGCAG 1 cut(s) 638
BspOI GCTAGC 1 cut(s) 369
BspQI GCTCTTC 1 cut(s) 116
BsrBI CCGCTC 1 cut(s) 126
BsrDI GCAATG 1 cut(s) 414
BsrI ACTGG 5 cut(s) 318, 346, 355, 605, 619
BssECI CCNNGG 2 cut(s) 309, 488
BssMI GATC 1 cut(s) 322
BssT1I CCWWGG 2 cut(s) 309, 488
Bst4CI ACNGT 1 cut(s) 676
Bst6I CTCTTC 2 cut(s) 41, 116
BstAPI GCANNNNNTGC 1 cut(s) 170
BstC8I GCNNGC 4 cut(s) 23, 367, 424, 606
BstDEI CTNAG 2 cut(s) 43, 571
BstDSI CCRYGG 1 cut(s) 309
BstF5I GGATG 3 cut(s) 249, 504, 557
BstKTI GATC 1 cut(s) 325
BstMAI GTCTC 1 cut(s) 38
BstMBI GATC 1 cut(s) 322
BstNSI RCATGY 1 cut(s) 382
BstSFI CTRYAG 1 cut(s) 634
BstV1I GCAGC 6 cut(s) 274, 283, 286, 289, 529, 538
BstXI CCANNNNNNTGG 2 cut(s) 482, 604
BsuRI GGCC 3 cut(s) 190, 248, 608
BtgI CCRYGG 1 cut(s) 309
BtgZI GCGATG 1 cut(s) 2
BtsCI GGATG 3 cut(s) 249, 504, 557
BtsIMutI CAGTG 3 cut(s) 325, 521, 730
Cac8I GCNNGC 4 cut(s) 23, 367, 424, 606
Cfr13I GGNCC 2 cut(s) 188, 485
CviAII CATG 5 cut(s) 209, 310, 379, 476, 688
DdeI CTNAG 2 cut(s) 43, 571
DpnI GATC 1 cut(s) 324
DpnII GATC 1 cut(s) 322
EaeI YGGCCR 2 cut(s) 246, 606
Eam1104I CTCTTC 2 cut(s) 41, 116
EarI CTCTTC 2 cut(s) 41, 116
Eco130I CCWWGG 2 cut(s) 309, 488
Eco47I GGWCC 1 cut(s) 485
EcoO109I RGGNCCY 1 cut(s) 188
EcoT14I CCWWGG 2 cut(s) 309, 488
ErhI CCWWGG 2 cut(s) 309, 488
FaeI CATG 5 cut(s) 212, 313, 382, 479, 691
FaiI YATR 9 cut(s) 197, 210, 251, 311, 372, 380, 477, 563, 689
FalI AAGNNNNNCTT 2 cut(s) 157, 189
FatI CATG 5 cut(s) 208, 309, 378, 475, 687
FbaI TGATCA 1 cut(s) 322
FblI GTMKAC 1 cut(s) 567
Fnu4HI GCNGC 8 cut(s) 133, 136, 288, 297, 300, 303, 543, 552
FokI GGATG 3 cut(s) 256, 491, 544
Fsp4HI GCNGC 8 cut(s) 133, 136, 288, 297, 300, 303, 543, 552
FspBI CTAG 3 cut(s) 366, 384, 530
GluI GCNGC 8 cut(s) 133, 136, 288, 297, 300, 303, 543, 552
GsaI CCCAGC 1 cut(s) 549
HaeIII GGCC 3 cut(s) 190, 248, 608
Hin1II CATG 5 cut(s) 212, 313, 382, 479, 691
HincII GTYRAC 1 cut(s) 144
HindII GTYRAC 1 cut(s) 144
HindIII AAGCTT 2 cut(s) 23, 63
HinfI GANTC 4 cut(s) 31, 140, 526, 697
HphI GGTGA 3 cut(s) 334, 638, 673
Hpy166II GTNNAC 3 cut(s) 144, 485, 568
Hpy188I TCNGA 1 cut(s) 111
Hpy188III TCNNGA 2 cut(s) 400, 694
Hpy8I GTNNAC 3 cut(s) 144, 485, 568
Hpy99I CGWCG 1 cut(s) 458
HpyAV CCTTC 3 cut(s) 341, 457, 694
HpyCH4III ACNGT 1 cut(s) 676
HpyCH4IV ACGT 1 cut(s) 642
HpyCH4V TGCA 5 cut(s) 462, 479, 554, 615, 636
HpyF3I CTNAG 2 cut(s) 43, 571
HpySE526I ACGT 1 cut(s) 642
Hsp92II CATG 5 cut(s) 212, 313, 382, 479, 691
Ksp22I TGATCA 1 cut(s) 322
Kzo9I GATC 1 cut(s) 322
LguI GCTCTTC 1 cut(s) 116
LmnI GCTCC 2 cut(s) 504, 599
Lsp1109I GCAGC 6 cut(s) 274, 283, 286, 289, 529, 538
LweI GCATC 1 cut(s) 471
MaeI CTAG 3 cut(s) 366, 384, 530
MaeII ACGT 1 cut(s) 642
MaeIII GTNAC 3 cut(s) 411, 670, 679
MalI GATC 1 cut(s) 324
MbiI CCGCTC 1 cut(s) 126
MboI GATC 1 cut(s) 322
MboII GAAGA 5 cut(s) 28, 133, 226, 331, 343
MhlI GDGCHC 1 cut(s) 438
MlsI TGGCCA 2 cut(s) 248, 608
MluCI AATT 2 cut(s) 48, 252
MluNI TGGCCA 2 cut(s) 248, 608
MlyI GAGTC 3 cut(s) 40, 149, 520
MmeI TCCRAC 1 cut(s) 621
MnlI CCTC 8 cut(s) 52, 97, 122, 154, 221, 233, 261, 512
Mox20I TGGCCA 2 cut(s) 248, 608
MroXI GAANNNNTTC 1 cut(s) 172
MscI TGGCCA 2 cut(s) 248, 608
MseI TTAA 1 cut(s) 6
MslI CAYNNNNRTG 2 cut(s) 480, 686
Msp20I TGGCCA 2 cut(s) 248, 608
NcoI CCATGG 1 cut(s) 309
NdeII GATC 1 cut(s) 322
NheI GCTAGC 1 cut(s) 365
NlaIII CATG 5 cut(s) 212, 313, 382, 479, 691
NlaIV GGNNCC 1 cut(s) 316
NmuCI GTSAC 2 cut(s) 411, 679
NspI RCATGY 1 cut(s) 382
PciSI GCTCTTC 1 cut(s) 116
PcsI WCGNNNNNNNCGW 1 cut(s) 462
PdmI GAANNNNTTC 1 cut(s) 172
PfeI GAWTC 1 cut(s) 697
PkrI GCNGC 8 cut(s) 134, 137, 289, 298, 301, 304, 544, 553
PleI GAGTC 3 cut(s) 39, 148, 520
PpsI GAGTC 3 cut(s) 39, 148, 520
PspFI CCCAGC 1 cut(s) 545
PspN4I GGNNCC 1 cut(s) 316
PspPI GGNCC 2 cut(s) 188, 485
PstI CTGCAG 1 cut(s) 638
RseI CAYNNNNRTG 2 cut(s) 480, 686
SapI GCTCTTC 1 cut(s) 116
SaqAI TTAA 1 cut(s) 6
SatI GCNGC 8 cut(s) 133, 136, 288, 297, 300, 303, 543, 552
Sau3AI GATC 1 cut(s) 322
Sau96I GGNCC 2 cut(s) 188, 485
SchI GAGTC 3 cut(s) 40, 149, 520
SduI GDGCHC 1 cut(s) 438
SfaNI GCATC 1 cut(s) 471
SfcI CTRYAG 1 cut(s) 634
SinI GGWCC 1 cut(s) 485
SmiMI CAYNNNNRTG 2 cut(s) 480, 686
SmlI CTYRAG 1 cut(s) 589
SmoI CTYRAG 1 cut(s) 589
Sse9I AATT 2 cut(s) 48, 252
SsiI CCGC 3 cut(s) 126, 132, 135
SspMI CTAG 3 cut(s) 366, 384, 530
StyI CCWWGG 2 cut(s) 309, 488
TaaI ACNGT 1 cut(s) 676
TaiI ACGT 1 cut(s) 645
TasI AATT 2 cut(s) 48, 252
TauI GCSGC 2 cut(s) 135, 138
TfiI GAWTC 1 cut(s) 697
Tru1I TTAA 1 cut(s) 6
Tru9I TTAA 1 cut(s) 6
TscAI CASTG 2 cut(s) 325, 528
TseFI GTSAC 2 cut(s) 411, 679
TseI GCWGC 6 cut(s) 287, 296, 299, 302, 542, 551
Tsp45I GTSAC 2 cut(s) 411, 679
TspDTI ATGAA 4 cut(s) 5, 134, 194, 676
TspGWI ACGGA 1 cut(s) 442
TspRI CASTG 2 cut(s) 325, 528
VpaK11BI GGWCC 1 cut(s) 485
XceI RCATGY 1 cut(s) 382
XcmI CCANNNNNNNNNTGG 1 cut(s) 256
XmiI GTMKAC 1 cut(s) 567
XmnI GAANNNNTTC 1 cut(s) 172
XspI CTAG 3 cut(s) 366, 384, 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.