FvH4_6g27511

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002692
Physical Location & Seq
Forward (+)
21156968 .. 21157889
922 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g27511.t1

Sequence Viewer

Length: 585 bp
ATGGCTAGAAATTATATTGTTGTTGGTGGTGGTGGTCATGGAGTGGGAGTAGGTCTCAGTATCTTGGCAGACAACTCTGGCGGCCTGCTTTTGGTTGGCATGGTTCTTATGTCTCTCTCACTCGTATCGATGGTCATATTTGCTTGTGGATCATCTGGCAAGAAAAGGAGGGGTGGCGGTGGTGGAGGCGGTTGTGGTGGTGGTGGTGATGGTGGTGGCGGTGGTGGTTGTGGAGGAGGTGCTGGTTGTGGAGGAGGTGGAGGTGGTGGTGGTGGTGGTTGTGGAGGAGGAGGTGGAGGTGGTGGTGGTGGTGGTGAGAAGACAACTATTCCAGTTGCAGAAGCTCTGGCTTTAAGAGACAGCTTTGTCAAAGCAAAGGAACAAGGCTACTGGAATATCCAAGTGGAAGGTGACTCAAAGCTGGTTATCGACGCTGTTAATGGAAGAATCAACATCCTTTGGAGACTTCTCAAGCTCATTCATGACATTAGAGATATTGCAAGATCCTTTTCTTCTGTTCGTTTTATGCATGTGTTTAGAGAAGTCAATTTTGTGGTAGACGTCATTGCTCATGAAGGACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

195

Amino Acids

18.93

Weight (kDa)

8.68

Isoelectric Point (pI)

55.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 105 - 192 1.1e-16 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 365
AatII GACGTC 1 cut(s) 564
AccI GTMKAC 1 cut(s) 558
AciI CCGC 4 cut(s) 81, 177, 189, 219
AclWI GGATC 2 cut(s) 157, 498
AcyI GRCGYC 1 cut(s) 561
AfiI CCNNNNNNNGG 1 cut(s) 91
AluBI AGCT 4 cut(s) 344, 363, 421, 475
AluI AGCT 4 cut(s) 344, 363, 421, 475
Alw26I GTCTC 4 cut(s) 59, 117, 351, 457
AlwI GGATC 2 cut(s) 157, 498
AoxI GGCC 1 cut(s) 82
AsuHPI GGTGA 3 cut(s) 218, 326, 422
BbsI GAAGAC 1 cut(s) 326
BccI CCATC 2 cut(s) 124, 203
BcoDI GTCTC 4 cut(s) 59, 117, 351, 457
BfaI CTAG 1 cut(s) 6
BisI GCNGC 1 cut(s) 82
BlsI GCNGC 1 cut(s) 83
BpiI GAAGAC 1 cut(s) 326
BplI GAGNNNNNCTC 2 cut(s) 39, 71
BpuEI CTTGAG 1 cut(s) 455
Bsa29I ATCGAT 1 cut(s) 128
BsaHI GRCGYC 1 cut(s) 561
BsaI GGTCTC 1 cut(s) 59
BsaXI ACNNNNNCTCC 2 cut(s) 33, 63
Bsc4I CCNNNNNNNGG 1 cut(s) 91
Bse1I ACTGG 2 cut(s) 332, 395
Bse3DI GCAATG 1 cut(s) 564
BseCI ATCGAT 1 cut(s) 128
BseGI GGATG 1 cut(s) 453
BseLI CCNNNNNNNGG 1 cut(s) 91
BseMI GCAATG 1 cut(s) 564
BseMII CTCAG 1 cut(s) 70
BseNI ACTGG 2 cut(s) 332, 395
BseRI GAGGAG 4 cut(s) 249, 267, 300, 303
BshFI GGCC 1 cut(s) 84
BshVI ATCGAT 1 cut(s) 128
BslI CCNNNNNNNGG 1 cut(s) 91
BsmAI GTCTC 4 cut(s) 59, 117, 351, 457
BsnI GGCC 1 cut(s) 84
Bso31I GGTCTC 1 cut(s) 59
Bsp143I GATC 2 cut(s) 149, 503
BspACI CCGC 4 cut(s) 81, 177, 189, 219
BspANI GGCC 1 cut(s) 84
BspCNI CTCAG 1 cut(s) 69
BspDI ATCGAT 1 cut(s) 128
BspHI TCATGA 2 cut(s) 481, 571
BspPI GGATC 2 cut(s) 157, 498
BspTNI GGTCTC 1 cut(s) 59
BsrDI GCAATG 1 cut(s) 564
BsrI ACTGG 2 cut(s) 332, 395
BssMI GATC 2 cut(s) 149, 503
BssNI GRCGYC 1 cut(s) 561
BstACI GRCGYC 1 cut(s) 561
BstC8I GCNNGC 1 cut(s) 86
BstDEI CTNAG 1 cut(s) 56
BstF5I GGATG 1 cut(s) 453
BstKTI GATC 2 cut(s) 152, 506
BstMAI GTCTC 4 cut(s) 59, 117, 351, 457
BstMBI GATC 2 cut(s) 149, 503
BstNSI RCATGY 1 cut(s) 533
BstV2I GAAGAC 1 cut(s) 326
BstX2I RGATCY 1 cut(s) 503
BstYI RGATCY 1 cut(s) 503
Bsu15I ATCGAT 1 cut(s) 128
BsuRI GGCC 1 cut(s) 84
BsuTUI ATCGAT 1 cut(s) 128
BtsCI GGATG 1 cut(s) 453
Cac8I GCNNGC 1 cut(s) 86
CciI TCATGA 2 cut(s) 481, 571
ClaI ATCGAT 1 cut(s) 128
CseI GACGC 1 cut(s) 440
CviAII CATG 5 cut(s) 38, 100, 482, 530, 572
CviJI RGCY 8 cut(s) 5, 84, 344, 350, 363, 387, 421, 475
CviKI_1 RGCY 8 cut(s) 5, 84, 344, 350, 363, 387, 421, 475
DdeI CTNAG 1 cut(s) 56
DpnI GATC 2 cut(s) 151, 505
DpnII GATC 2 cut(s) 149, 503
DrdI GACNNNNNNGTC 1 cut(s) 365
DseDI GACNNNNNNGTC 1 cut(s) 365
Eco31I GGTCTC 1 cut(s) 59
EcoT22I ATGCAT 1 cut(s) 531
FaeI CATG 5 cut(s) 41, 103, 485, 533, 575
FaiI YATR 9 cut(s) 15, 39, 101, 110, 137, 483, 527, 531, 573
FatI CATG 5 cut(s) 37, 99, 481, 529, 571
FblI GTMKAC 1 cut(s) 558
Fnu4HI GCNGC 1 cut(s) 82
FokI GGATG 1 cut(s) 440
Fsp4HI GCNGC 1 cut(s) 82
FspBI CTAG 1 cut(s) 6
GluI GCNGC 1 cut(s) 82
HaeIII GGCC 1 cut(s) 84
HgaI GACGC 1 cut(s) 440
Hin1I GRCGYC 1 cut(s) 561
Hin1II CATG 5 cut(s) 41, 103, 485, 533, 575
HinfI GANTC 2 cut(s) 413, 447
HphI GGTGA 3 cut(s) 218, 326, 422
Hpy166II GTNNAC 1 cut(s) 559
Hpy188III TCNNGA 2 cut(s) 482, 572
Hpy8I GTNNAC 1 cut(s) 559
Hpy99I CGWCG 1 cut(s) 434
HpyAV CCTTC 2 cut(s) 401, 569
HpyCH4IV ACGT 1 cut(s) 561
HpyCH4V TGCA 3 cut(s) 338, 500, 529
HpyF3I CTNAG 1 cut(s) 56
HpySE526I ACGT 1 cut(s) 561
Hsp92I GRCGYC 1 cut(s) 561
Hsp92II CATG 5 cut(s) 41, 103, 485, 533, 575
Kzo9I GATC 2 cut(s) 149, 503
LpnPI CCDG 8 cut(s) 63, 98, 141, 228, 332, 345, 376, 407
MaeI CTAG 1 cut(s) 6
MaeII ACGT 1 cut(s) 561
MaeIII GTNAC 1 cut(s) 410
MalI GATC 2 cut(s) 151, 505
MboI GATC 2 cut(s) 149, 503
MboII GAAGA 3 cut(s) 331, 456, 504
MflI RGATCY 1 cut(s) 503
MluCI AATT 2 cut(s) 10, 547
MlyI GAGTC 1 cut(s) 407
Mph1103I ATGCAT 1 cut(s) 531
MseI TTAA 2 cut(s) 353, 438
NdeII GATC 2 cut(s) 149, 503
NlaIII CATG 5 cut(s) 41, 103, 485, 533, 575
NmuCI GTSAC 1 cut(s) 410
NsiI ATGCAT 1 cut(s) 531
NspI RCATGY 1 cut(s) 533
PagI TCATGA 2 cut(s) 481, 571
PfeI GAWTC 1 cut(s) 447
PkrI GCNGC 1 cut(s) 83
PleI GAGTC 1 cut(s) 407
PpsI GAGTC 1 cut(s) 407
PsuI RGATCY 1 cut(s) 503
SaqAI TTAA 2 cut(s) 353, 438
SatI GCNGC 1 cut(s) 82
Sau3AI GATC 2 cut(s) 149, 503
SchI GAGTC 1 cut(s) 407
SmlI CTYRAG 1 cut(s) 470
SmoI CTYRAG 1 cut(s) 470
Sse9I AATT 2 cut(s) 10, 547
SsiI CCGC 4 cut(s) 81, 177, 189, 219
SspMI CTAG 1 cut(s) 6
TaiI ACGT 1 cut(s) 564
TaqI TCGA 2 cut(s) 128, 429
TasI AATT 2 cut(s) 10, 547
TauI GCSGC 1 cut(s) 84
TfiI GAWTC 1 cut(s) 447
Tru1I TTAA 2 cut(s) 353, 438
Tru9I TTAA 2 cut(s) 353, 438
TseFI GTSAC 1 cut(s) 410
Tsp45I GTSAC 1 cut(s) 410
TspDTI ATGAA 1 cut(s) 470
XceI RCATGY 1 cut(s) 533
XmiI GTMKAC 1 cut(s) 558
XspI CTAG 1 cut(s) 6
ZraI GACGTC 1 cut(s) 562
Zsp2I ATGCAT 1 cut(s) 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.