FvH4_6g30270

Asp/Glu/Hydantoin racemase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002475
Physical Location & Seq
Forward (+)
23361712 .. 23364388
2677 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g30270.t3

Sequence Viewer

Length: 969 bp
ATGACAACTCCTTCTCACACTTTGAACTATCCTTTTCACAAATTGGCTCCTGTAGATACACATAAAACCCTCTACAAGACGAGGTGGAATCAGGGTTTCGCTGCTCTTTCCCCAGTTCTCTTACACGCAGAAAGTGGGAAGTTTCCGAAGTATAAGACGAATTCCGGTTTGATTAAGGCTTGCAGCGATTCTTTCCTGGGTGTACCCAATACAATTGGTATAATTGGAGGGGTATCTGTTGATTCTACTGTATATTTTTTGAAAAAACTAGTTCACTGGAGTAAGAATGGAGAAGGTTGTCCTCCTTTTGTTCTATGCTGTGATCCTGTGTTAAATAAAGAGCTGTCATTGATACAAAGAAGCTCAGCTCCTTTGCTTAACAATAAATATGAACGAACTACACGAGATCCTACTTCAGTTGTGGAGAATCTGAGGAATAAGAGGATTTATCTTGAACATTCTGGAGCTTGTTGCATAGTAATGCCTTGTCATATGTCACATTCTTGGCATAATGAAATTTCTGAGGGATGTTCTGTTCCTTTCCTCCATATGGGTGAGTGTGTTGTCAAGGAGCTCAAGGAAGCGAAGTTGAGGCCGCTTGAAGCTGGGAGTCCTTTGCGGATTGGATTGCTTGCTACTAAAGCAATTTTGACAGCAGGATTCTATCAGGAGAAATTGAAAAGCGAGGGGTTTGAGGTTGTGTTGCCAGATGAAGCGACCATGGAACACACTATCATCCCTGCTATTGAAGCTTTAAACAGAAAAGACATAGAGGGATCACAGAATCTTTTGAGGATTGCACTCCAGGTTCTTCTAGTGAGGGCTGTGAACTCTGTAATTCTTGCCTCTGATGATATCCGGGATCTTCTTCCCCAGGATGATCCTCTTCTTGAGAAATGCATTGATCCTATGGATGCATTGGCTCGGTCAACCATAAAATTGGCTACATCTGTTGAAAATGGTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asp_Glu_race

Protein Analysis

323

Amino Acids

35.55

Weight (kDa)

7.14

Isoelectric Point (pI)

48.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp_Glu_race PF01177 75 - 241 8.7e-14 Asp/Glu/Hydantoin racemase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 596, 619
AclWI GGATC 6 cut(s) 317, 401, 784, 870, 875, 899
AcsI RAATTY 2 cut(s) 160, 516
AcuI CTGAAG 1 cut(s) 399
AfaI GTAC 2 cut(s) 204, 964
AfiI CCNNNNNNNGG 1 cut(s) 550
AgsI TTSAA 7 cut(s) 25, 262, 455, 602, 679, 749, 956
AhlI ACTAGT 1 cut(s) 268
AjnI CCWGG 3 cut(s) 195, 804, 873
AloI GAACNNNNNNTCC 2 cut(s) 519, 551
AluBI AGCT 7 cut(s) 343, 363, 368, 467, 574, 605, 752
AluI AGCT 7 cut(s) 343, 363, 368, 467, 574, 605, 752
Alw21I GWGCWC 1 cut(s) 576
AlwI GGATC 6 cut(s) 317, 401, 784, 870, 875, 899
AoxI GGCC 1 cut(s) 593
ApeKI GCWGC 2 cut(s) 101, 183
ApoI RAATTY 2 cut(s) 160, 516
AsuC2I CCSGG 1 cut(s) 860
AsuHPI GGTGA 1 cut(s) 566
BanII GRGCYC 1 cut(s) 576
BauI CACGAG 1 cut(s) 402
Bbv12I GWGCWC 1 cut(s) 576
BbvI GCAGC 2 cut(s) 88, 195
BciT130I CCWGG 3 cut(s) 197, 806, 875
BcnI CCSGG 1 cut(s) 860
BcuI ACTAGT 1 cut(s) 268
BfaI CTAG 2 cut(s) 269, 815
BfmI CTRYAG 1 cut(s) 51
BisI GCNGC 3 cut(s) 102, 184, 596
BlpI GCTNAGC 1 cut(s) 364
BlsI GCNGC 3 cut(s) 103, 185, 597
Bme1390I CCNGG 4 cut(s) 197, 806, 860, 875
BmiI GGNNCC 1 cut(s) 48
BmrFI CCNGG 4 cut(s) 197, 806, 860, 875
BmrI ACTGGG 1 cut(s) 107
BmsI GCATC 1 cut(s) 904
BmuI ACTGGG 1 cut(s) 107
BpmI CTGGAG 3 cut(s) 298, 483, 788
Bpu1102I GCTNAGC 1 cut(s) 364
BpuEI CTTGAG 2 cut(s) 560, 911
BpuMI CCSGG 1 cut(s) 860
BsaJI CCNNGG 3 cut(s) 196, 720, 873
BsaWI WCCGGW 1 cut(s) 164
Bsc4I CCNNNNNNNGG 1 cut(s) 550
Bse1I ACTGG 2 cut(s) 113, 281
BseBI CCWGG 3 cut(s) 197, 806, 875
BseDI CCNNGG 3 cut(s) 196, 720, 873
BseGI GGATG 4 cut(s) 533, 735, 883, 919
BseLI CCNNNNNNNGG 1 cut(s) 550
BseMII CTCAG 3 cut(s) 378, 422, 513
BseNI ACTGG 2 cut(s) 113, 281
BseXI GCAGC 2 cut(s) 88, 195
BseYI CCCAGC 1 cut(s) 605
BshFI GGCC 1 cut(s) 595
BsiHKAI GWGCWC 1 cut(s) 576
BsiSI CCGG 2 cut(s) 165, 859
BslI CCNNNNNNNGG 1 cut(s) 550
BsnI GGCC 1 cut(s) 595
Bsp1286I GDGCHC 1 cut(s) 576
Bsp143I GATC 6 cut(s) 322, 406, 776, 862, 880, 904
Bsp1720I GCTNAGC 1 cut(s) 364
Bsp19I CCATGG 1 cut(s) 720
BspACI CCGC 2 cut(s) 596, 619
BspANI GGCC 1 cut(s) 595
BspCNI CTCAG 3 cut(s) 377, 423, 514
BspLI GGNNCC 1 cut(s) 48
BspPI GGATC 6 cut(s) 317, 401, 784, 870, 875, 899
BsrI ACTGG 2 cut(s) 113, 281
BssECI CCNNGG 3 cut(s) 196, 720, 873
BssMI GATC 6 cut(s) 322, 406, 776, 862, 880, 904
BssSI CACGAG 1 cut(s) 402
BssT1I CCWWGG 1 cut(s) 720
Bst2BI CACGAG 1 cut(s) 402
Bst2UI CCWGG 3 cut(s) 197, 806, 875
Bst4CI ACNGT 1 cut(s) 250
Bst6I CTCTTC 1 cut(s) 891
BstC8I GCNNGC 2 cut(s) 181, 633
BstDEI CTNAG 3 cut(s) 364, 431, 522
BstDSI CCRYGG 1 cut(s) 720
BstF5I GGATG 4 cut(s) 533, 735, 883, 919
BstKTI GATC 6 cut(s) 325, 409, 779, 865, 883, 907
BstMBI GATC 6 cut(s) 322, 406, 776, 862, 880, 904
BstMWI GCNNNNNNNGC 2 cut(s) 641, 749
BstNI CCWGG 3 cut(s) 197, 806, 875
BstSCI CCNGG 4 cut(s) 195, 804, 858, 873
BstSFI CTRYAG 1 cut(s) 51
BstV1I GCAGC 2 cut(s) 88, 195
BstX2I RGATCY 2 cut(s) 406, 862
BstXI CCANNNNNNTGG 1 cut(s) 940
BstYI RGATCY 2 cut(s) 406, 862
BsuRI GGCC 1 cut(s) 595
BtgI CCRYGG 1 cut(s) 720
BtsCI GGATG 4 cut(s) 533, 735, 883, 919
BtsIMutI CAGTG 1 cut(s) 274
Cac8I GCNNGC 2 cut(s) 181, 633
Csp6I GTAC 2 cut(s) 203, 963
CviAII CATG 2 cut(s) 721, 966
CviQI GTAC 2 cut(s) 203, 963
DdeI CTNAG 3 cut(s) 364, 431, 522
DpnI GATC 6 cut(s) 324, 408, 778, 864, 882, 906
DpnII GATC 6 cut(s) 322, 406, 776, 862, 880, 904
DraI TTTAAA 1 cut(s) 756
Eam1104I CTCTTC 1 cut(s) 891
EarI CTCTTC 1 cut(s) 891
Ecl136II GAGCTC 1 cut(s) 574
Eco130I CCWWGG 1 cut(s) 720
Eco24I GRGCYC 1 cut(s) 576
Eco32I GATATC 1 cut(s) 856
Eco53kI GAGCTC 1 cut(s) 574
Eco57I CTGAAG 1 cut(s) 399
EcoICRI GAGCTC 1 cut(s) 574
EcoRI GAATTC 1 cut(s) 160
EcoRII CCWGG 3 cut(s) 195, 804, 873
EcoRV GATATC 1 cut(s) 856
EcoT14I CCWWGG 1 cut(s) 720
EcoT22I ATGCAT 2 cut(s) 902, 919
EcoT38I GRGCYC 1 cut(s) 576
ErhI CCWWGG 1 cut(s) 720
FaeI CATG 2 cut(s) 724, 969
FatI CATG 2 cut(s) 720, 965
FauNDI CATATG 2 cut(s) 492, 549
Fnu4HI GCNGC 3 cut(s) 102, 184, 596
FokI GGATG 4 cut(s) 540, 722, 890, 926
FriOI GRGCYC 1 cut(s) 576
Fsp4HI GCNGC 3 cut(s) 102, 184, 596
FspBI CTAG 2 cut(s) 269, 815
GluI GCNGC 3 cut(s) 102, 184, 596
GsaI CCCAGC 1 cut(s) 609
GsuI CTGGAG 3 cut(s) 298, 483, 788
HaeIII GGCC 1 cut(s) 595
HapII CCGG 2 cut(s) 165, 859
Hin1II CATG 2 cut(s) 724, 969
HincII GTYRAC 1 cut(s) 930
HindII GTYRAC 1 cut(s) 930
HindIII AAGCTT 1 cut(s) 750
HinfI GANTC 7 cut(s) 88, 188, 242, 427, 610, 660, 784
HpaII CCGG 2 cut(s) 165, 859
HphI GGTGA 1 cut(s) 566
Hpy166II GTNNAC 4 cut(s) 203, 274, 829, 930
Hpy188I TCNGA 4 cut(s) 147, 432, 523, 850
Hpy188III TCNNGA 4 cut(s) 452, 462, 668, 890
Hpy8I GTNNAC 4 cut(s) 203, 274, 829, 930
HpyAV CCTTC 2 cut(s) 21, 287
HpyCH4III ACNGT 1 cut(s) 250
HpyCH4V TGCA 5 cut(s) 183, 474, 800, 900, 917
HpyF10VI GCNNNNNNNGC 2 cut(s) 641, 749
HpyF3I CTNAG 3 cut(s) 364, 431, 522
Hsp92II CATG 2 cut(s) 724, 969
Kzo9I GATC 6 cut(s) 322, 406, 776, 862, 880, 904
LmnI GCTCC 4 cut(s) 52, 373, 464, 571
Lsp1109I GCAGC 2 cut(s) 88, 195
LweI GCATC 1 cut(s) 904
MaeI CTAG 2 cut(s) 269, 815
MaeIII GTNAC 1 cut(s) 495
MalI GATC 6 cut(s) 324, 408, 778, 864, 882, 906
MboI GATC 6 cut(s) 322, 406, 776, 862, 880, 904
MboII GAAGA 4 cut(s) 803, 857, 860, 878
MfeI CAATTG 1 cut(s) 213
MflI RGATCY 2 cut(s) 406, 862
MhlI GDGCHC 1 cut(s) 576
MluCI AATT 9 cut(s) 41, 160, 213, 222, 516, 645, 674, 837, 938
MlyI GAGTC 1 cut(s) 619
Mph1103I ATGCAT 2 cut(s) 902, 919
MseI TTAA 4 cut(s) 174, 332, 378, 755
MslI CAYNNNNRTG 2 cut(s) 479, 552
MspI CCGG 2 cut(s) 165, 859
MspR9I CCNGG 4 cut(s) 197, 806, 860, 875
MunI CAATTG 1 cut(s) 213
MvaI CCWGG 3 cut(s) 197, 806, 875
MwoI GCNNNNNNNGC 2 cut(s) 641, 749
NciI CCSGG 1 cut(s) 860
NcoI CCATGG 1 cut(s) 720
NdeI CATATG 2 cut(s) 492, 549
NdeII GATC 6 cut(s) 322, 406, 776, 862, 880, 904
NlaIII CATG 2 cut(s) 724, 969
NlaIV GGNNCC 1 cut(s) 48
NmuCI GTSAC 1 cut(s) 495
NsiI ATGCAT 2 cut(s) 902, 919
PcsI WCGNNNNNNNCGW 1 cut(s) 400
PfeI GAWTC 6 cut(s) 88, 188, 242, 427, 660, 784
PfoI TCCNGGA 1 cut(s) 858
PkrI GCNGC 3 cut(s) 103, 185, 597
PleI GAGTC 1 cut(s) 618
PpsI GAGTC 1 cut(s) 618
Psp124BI GAGCTC 1 cut(s) 576
Psp6I CCWGG 3 cut(s) 195, 804, 873
PspFI CCCAGC 1 cut(s) 605
PspGI CCWGG 3 cut(s) 195, 804, 873
PspN4I GGNNCC 1 cut(s) 48
PsuI RGATCY 2 cut(s) 406, 862
RsaI GTAC 2 cut(s) 204, 964
RsaNI GTAC 2 cut(s) 203, 963
RseI CAYNNNNRTG 2 cut(s) 479, 552
SacI GAGCTC 1 cut(s) 576
SaqAI TTAA 4 cut(s) 174, 332, 378, 755
SatI GCNGC 3 cut(s) 102, 184, 596
Sau3AI GATC 6 cut(s) 322, 406, 776, 862, 880, 904
SchI GAGTC 1 cut(s) 619
ScrFI CCNGG 4 cut(s) 197, 806, 860, 875
SduI GDGCHC 1 cut(s) 576
SfaNI GCATC 1 cut(s) 904
SfcI CTRYAG 1 cut(s) 51
SmiMI CAYNNNNRTG 2 cut(s) 479, 552
SmlI CTYRAG 2 cut(s) 575, 890
SmoI CTYRAG 2 cut(s) 575, 890
SpeI ACTAGT 1 cut(s) 268
Sse9I AATT 9 cut(s) 41, 160, 213, 222, 516, 645, 674, 837, 938
SsiI CCGC 2 cut(s) 596, 619
SspMI CTAG 2 cut(s) 269, 815
SstI GAGCTC 1 cut(s) 576
StyD4I CCNGG 4 cut(s) 195, 804, 858, 873
StyI CCWWGG 1 cut(s) 720
TaaI ACNGT 1 cut(s) 250
TaqII GACCGA 1 cut(s) 915
TasI AATT 9 cut(s) 41, 160, 213, 222, 516, 645, 674, 837, 938
TauI GCSGC 1 cut(s) 598
TfiI GAWTC 6 cut(s) 88, 188, 242, 427, 660, 784
Tru1I TTAA 4 cut(s) 174, 332, 378, 755
Tru9I TTAA 4 cut(s) 174, 332, 378, 755
TscAI CASTG 1 cut(s) 281
TseFI GTSAC 1 cut(s) 495
TseI GCWGC 2 cut(s) 101, 183
Tsp45I GTSAC 1 cut(s) 495
TspDTI ATGAA 3 cut(s) 405, 528, 726
TspRI CASTG 1 cut(s) 281
XapI RAATTY 2 cut(s) 160, 516
XspI CTAG 2 cut(s) 269, 815
Zsp2I ATGCAT 2 cut(s) 902, 919
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.