pycom02g02920

Asp/Glu/Hydantoin racemase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0002475
Physical Location & Seq
Forward (+)
1873128 .. 1874274
1147 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g02920.1

Sequence Viewer

Length: 999 bp
ATGGTTGATGCAGGGATGACAATGTCCTTCCACACACAAAACCGTCCACCTCTTCTTTCTGGACTTTTGACCAAAAATAGGACACAAAACAGAACCAAATCAAGCTCTTCTGTAGCTGCAATGCAAGCATCTTCAGTTCTGCAAACCAATGAGAGCAGAAATCTACCTGAATCGAAGAAAGTTCGGAATTCGGGCTTACCTCTAACAAGATGTCACGTACTGAGCCAACCAAACACTGTCGGCATCATTGGAGGGGTGTCTGTTTATTCAAGCCTCCTTTTCTTGGAGAAGCTTGTGTGGTGGAGTCTAAAAAATGGAGGAGAATGCCCGCCCTTCATCGTGTGCAGCGATCCAACATTGTATAAGGAGCTTCCGATTCGTAGTTTGTTTCATTCACACACAAGAGGAAGTACCAGTAGTGCTCAAATCCTATCAAATAACTGGCCTATCATCGAGAGCTTGTGTAGCAAGAGGGCGTTCCTCCAGCACTCTGGCGCTCGATGCATAGTCATGCCGTGTCATCTGTCCCATGCGTGGCACGACCAGATATCTGTGGACTGTTCTCTGCGCTTCCTTCATATCGGTGAGTGTGTTGCCCTGGAGCTCAGGGAAGCAAAGTTGAAGCCACTGGAAAAAGGAAGTAATGTAAGGATTGGAGTGCTTGCTGCAGATGCAACTTTAATGGCTGGTTTTTACCAAGAAAAGCTACAAAGTCAGGGGTTTGATGTTGTGGTACCTGACAAGCAAACCATGGAGCATGTGGTGATTCCCGCAATTGAAGCGATAAAACGACGGGACATGAAGGGGGCACGAAACCTGTTAAGCATTGCAGTCCAGGTCCTGCTGGTGAGGGCGGTGAACACTGTGATCATTGCTTCGGACGAGTTGCAGGTTGTTCTGCCTCCGGGCGATCCTCTTCTTAAGAAATGTATCAACCCCATGGACGCTTTGGCTAGGTCGACAATAAAATGGGCAAAGGCTACAAGCAACGGTGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asp_Glu_race

Protein Analysis

333

Amino Acids

36.36

Weight (kDa)

9.41

Isoelectric Point (pI)

42.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp_Glu_race PF01177 83 - 320 5.9e-19 Asp/Glu/Hydantoin racemase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 880
Acc65I GGTACC 1 cut(s) 733
AccB1I GGYRCC 1 cut(s) 733
AccI GTMKAC 1 cut(s) 959
AciI CCGC 3 cut(s) 329, 771, 854
AclWI GGATC 2 cut(s) 344, 905
AcsI RAATTY 1 cut(s) 187
AcuI CTGAAG 1 cut(s) 117
AfaI GTAC 3 cut(s) 219, 412, 735
AfiI CCNNNNNNNGG 3 cut(s) 78, 283, 534
AflII CTTAAG 1 cut(s) 920
AgsI TTSAA 3 cut(s) 270, 622, 779
AjnI CCWGG 2 cut(s) 597, 834
AluBI AGCT 7 cut(s) 105, 116, 292, 370, 459, 604, 706
AluI AGCT 7 cut(s) 105, 116, 292, 370, 459, 604, 706
Alw21I GWGCWC 2 cut(s) 424, 606
AlwI GGATC 2 cut(s) 344, 905
AlwNI CAGNNNCTG 1 cut(s) 841
AoxI GGCC 1 cut(s) 443
ApeKI GCWGC 3 cut(s) 116, 345, 665
ApoI RAATTY 1 cut(s) 187
Asp718I GGTACC 1 cut(s) 733
AspLEI GCGC 2 cut(s) 497, 570
AspS9I GGNCC 1 cut(s) 838
AsuC2I CCSGG 1 cut(s) 906
AsuHPI GGTGA 4 cut(s) 596, 775, 859, 868
AvaII GGWCC 1 cut(s) 838
BaeGI GKGCMC 1 cut(s) 811
BanI GGYRCC 1 cut(s) 733
BanII GRGCYC 1 cut(s) 606
Bbv12I GWGCWC 2 cut(s) 424, 606
BbvI GCAGC 3 cut(s) 103, 357, 652
BceAI ACGGC 1 cut(s) 499
BciT130I CCWGG 2 cut(s) 599, 836
BclI TGATCA 1 cut(s) 867
BcnI CCSGG 1 cut(s) 906
BfaI CTAG 1 cut(s) 954
BfmI CTRYAG 2 cut(s) 111, 666
BfoI RGCGCY 1 cut(s) 498
BfrI CTTAAG 1 cut(s) 920
BfuAI ACCTGC 1 cut(s) 880
BisI GCNGC 3 cut(s) 117, 346, 666
BlsI GCNGC 3 cut(s) 118, 347, 667
Bme1390I CCNGG 3 cut(s) 599, 836, 906
Bme18I GGWCC 1 cut(s) 838
BmgT120I GGNCC 1 cut(s) 838
BmiI GGNNCC 1 cut(s) 735
BmrFI CCNGG 3 cut(s) 599, 836, 906
BmsI GCATC 4 cut(s) 137, 252, 491, 661
BpmI CTGGAG 2 cut(s) 467, 620
Bpu10I CCTNAGC 1 cut(s) 605
BpuMI CCSGG 1 cut(s) 906
BsaAI YACGTR 1 cut(s) 217
BsaJI CCNNGG 3 cut(s) 597, 750, 939
Bsc4I CCNNNNNNNGG 3 cut(s) 78, 283, 534
Bse1I ACTGG 3 cut(s) 414, 446, 633
Bse3DI GCAATG 3 cut(s) 126, 825, 870
BseBI CCWGG 2 cut(s) 599, 836
BseDI CCNNGG 3 cut(s) 597, 750, 939
BseGI GGATG 1 cut(s) 21
BseLI CCNNNNNNNGG 3 cut(s) 78, 283, 534
BseMI GCAATG 3 cut(s) 126, 825, 870
BseMII CTCAG 2 cut(s) 212, 619
BseNI ACTGG 3 cut(s) 414, 446, 633
BseRI GAGGAG 1 cut(s) 333
BseSI GKGCMC 1 cut(s) 811
BseXI GCAGC 3 cut(s) 103, 357, 652
BsgI GTGCAG 1 cut(s) 364
BshFI GGCC 1 cut(s) 445
BshNI GGYRCC 1 cut(s) 733
BsiHKAI GWGCWC 2 cut(s) 424, 606
BsiSI CCGG 1 cut(s) 905
BslFI GGGAC 2 cut(s) 511, 809
BslI CCNNNNNNNGG 3 cut(s) 78, 283, 534
BsmFI GGGAC 2 cut(s) 511, 809
BsmI GAATGC 1 cut(s) 329
BsnI GGCC 1 cut(s) 445
Bsp1286I GDGCHC 3 cut(s) 424, 606, 811
Bsp143I GATC 3 cut(s) 349, 867, 910
Bsp19I CCATGG 2 cut(s) 750, 939
BspACI CCGC 3 cut(s) 329, 771, 854
BspANI GGCC 1 cut(s) 445
BspCNI CTCAG 2 cut(s) 213, 618
BspLI GGNNCC 1 cut(s) 735
BspMAI CTGCAG 1 cut(s) 670
BspMI ACCTGC 1 cut(s) 880
BspPI GGATC 2 cut(s) 344, 905
BspQI GCTCTTC 1 cut(s) 112
BspT107I GGYRCC 1 cut(s) 733
BspTI CTTAAG 1 cut(s) 920
BsrDI GCAATG 3 cut(s) 126, 825, 870
BsrI ACTGG 3 cut(s) 414, 446, 633
BssECI CCNNGG 3 cut(s) 597, 750, 939
BssMI GATC 3 cut(s) 349, 867, 910
BssT1I CCWWGG 2 cut(s) 750, 939
Bst2UI CCWGG 2 cut(s) 599, 836
Bst4CI ACNGT 5 cut(s) 44, 238, 560, 865, 992
Bst6I CTCTTC 3 cut(s) 57, 112, 921
BstAFI CTTAAG 1 cut(s) 920
BstBAI YACGTR 1 cut(s) 217
BstC8I GCNNGC 3 cut(s) 126, 329, 663
BstDEI CTNAG 2 cut(s) 221, 605
BstDSI CCRYGG 2 cut(s) 750, 939
BstF5I GGATG 1 cut(s) 21
BstH2I RGCGCY 1 cut(s) 498
BstHHI GCGC 2 cut(s) 497, 570
BstKTI GATC 3 cut(s) 352, 870, 913
BstMBI GATC 3 cut(s) 349, 867, 910
BstMWI GCNNNNNNNGC 5 cut(s) 125, 465, 501, 671, 779
BstNI CCWGG 2 cut(s) 599, 836
BstNSI RCATGY 1 cut(s) 761
BstSCI CCNGG 3 cut(s) 597, 834, 904
BstSFI CTRYAG 2 cut(s) 111, 666
BstSLI GKGCMC 1 cut(s) 811
BstV1I GCAGC 3 cut(s) 103, 357, 652
BstXI CCANNNNNNTGG 1 cut(s) 491
BsuRI GGCC 1 cut(s) 445
BtgI CCRYGG 2 cut(s) 750, 939
BtsCI GGATG 1 cut(s) 21
BtsIMutI CAGTG 3 cut(s) 234, 626, 861
BveI ACCTGC 1 cut(s) 880
Cac8I GCNNGC 3 cut(s) 126, 329, 663
CaiI CAGNNNCTG 1 cut(s) 841
CfoI GCGC 2 cut(s) 497, 570
Cfr13I GGNCC 1 cut(s) 838
CseI GACGC 1 cut(s) 953
Csp6I GTAC 3 cut(s) 218, 411, 734
CviAII CATG 6 cut(s) 511, 530, 751, 758, 799, 940
CviQI GTAC 3 cut(s) 218, 411, 734
DdeI CTNAG 2 cut(s) 221, 605
DpnI GATC 3 cut(s) 351, 869, 912
DpnII GATC 3 cut(s) 349, 867, 910
Eam1104I CTCTTC 3 cut(s) 57, 112, 921
EarI CTCTTC 3 cut(s) 57, 112, 921
Ecl136II GAGCTC 1 cut(s) 604
Eco130I CCWWGG 2 cut(s) 750, 939
Eco24I GRGCYC 1 cut(s) 606
Eco32I GATATC 1 cut(s) 549
Eco47I GGWCC 1 cut(s) 838
Eco53kI GAGCTC 1 cut(s) 604
Eco57I CTGAAG 1 cut(s) 117
EcoICRI GAGCTC 1 cut(s) 604
EcoO109I RGGNCCY 1 cut(s) 838
EcoRI GAATTC 1 cut(s) 187
EcoRII CCWGG 2 cut(s) 597, 834
EcoRV GATATC 1 cut(s) 549
EcoT14I CCWWGG 2 cut(s) 750, 939
EcoT22I ATGCAT 1 cut(s) 506
EcoT38I GRGCYC 1 cut(s) 606
ErhI CCWWGG 2 cut(s) 750, 939
FaeI CATG 6 cut(s) 514, 533, 754, 761, 802, 943
FaiI YATR 9 cut(s) 363, 506, 512, 531, 579, 752, 759, 800, 941
FaqI GGGAC 2 cut(s) 511, 809
FatI CATG 6 cut(s) 510, 529, 750, 757, 798, 939
FauI CCCGC 2 cut(s) 336, 778
FbaI TGATCA 1 cut(s) 867
FblI GTMKAC 1 cut(s) 959
Fnu4HI GCNGC 3 cut(s) 117, 346, 666
FokI GGATG 1 cut(s) 28
FriOI GRGCYC 1 cut(s) 606
Fsp4HI GCNGC 3 cut(s) 117, 346, 666
FspBI CTAG 1 cut(s) 954
GlaI GCGC 2 cut(s) 496, 569
GluI GCNGC 3 cut(s) 117, 346, 666
GsuI CTGGAG 2 cut(s) 467, 620
HaeII RGCGCY 1 cut(s) 498
HaeIII GGCC 1 cut(s) 445
HapII CCGG 1 cut(s) 905
HgaI GACGC 1 cut(s) 953
HhaI GCGC 2 cut(s) 497, 570
Hin1II CATG 6 cut(s) 514, 533, 754, 761, 802, 943
Hin6I GCGC 2 cut(s) 495, 568
HinP1I GCGC 2 cut(s) 495, 568
HincII GTYRAC 1 cut(s) 960
HindII GTYRAC 1 cut(s) 960
HindIII AAGCTT 1 cut(s) 290
HinfI GANTC 4 cut(s) 170, 304, 376, 766
HpaII CCGG 1 cut(s) 905
HphI GGTGA 4 cut(s) 596, 775, 859, 868
Hpy166II GTNNAC 4 cut(s) 47, 556, 859, 960
Hpy188I TCNGA 3 cut(s) 186, 375, 880
Hpy188III TCNNGA 2 cut(s) 60, 454
Hpy8I GTNNAC 4 cut(s) 47, 556, 859, 960
Hpy99I CGWCG 1 cut(s) 795
HpyAV CCTTC 4 cut(s) 37, 343, 584, 796
HpyCH4III ACNGT 5 cut(s) 44, 238, 560, 865, 992
HpyCH4IV ACGT 1 cut(s) 216
HpyF10VI GCNNNNNNNGC 5 cut(s) 125, 465, 501, 671, 779
HpyF3I CTNAG 2 cut(s) 221, 605
HpySE526I ACGT 1 cut(s) 216
Hsp92II CATG 6 cut(s) 514, 533, 754, 761, 802, 943
HspAI GCGC 2 cut(s) 495, 568
KpnI GGTACC 1 cut(s) 737
Ksp22I TGATCA 1 cut(s) 867
Kzo9I GATC 3 cut(s) 349, 867, 910
LguI GCTCTTC 1 cut(s) 112
LmnI GCTCC 3 cut(s) 367, 601, 754
Lsp1109I GCAGC 3 cut(s) 103, 357, 652
LweI GCATC 4 cut(s) 137, 252, 491, 661
MaeI CTAG 1 cut(s) 954
MaeII ACGT 1 cut(s) 216
MaeIII GTNAC 1 cut(s) 212
MalI GATC 3 cut(s) 351, 869, 912
MboI GATC 3 cut(s) 349, 867, 910
MboII GAAGA 5 cut(s) 44, 99, 123, 187, 908
MfeI CAATTG 1 cut(s) 774
MhlI GDGCHC 3 cut(s) 424, 606, 811
MluCI AATT 2 cut(s) 187, 774
MlyI GAGTC 1 cut(s) 313
MmeI TCCRAC 1 cut(s) 377
Mph1103I ATGCAT 1 cut(s) 506
MseI TTAA 3 cut(s) 680, 821, 921
MslI CAYNNNNRTG 2 cut(s) 509, 582
MspCI CTTAAG 1 cut(s) 920
MspI CCGG 1 cut(s) 905
MspR9I CCNGG 3 cut(s) 599, 836, 906
MunI CAATTG 1 cut(s) 774
Mva1269I GAATGC 1 cut(s) 329
MvaI CCWGG 2 cut(s) 599, 836
MwoI GCNNNNNNNGC 5 cut(s) 125, 465, 501, 671, 779
NciI CCSGG 1 cut(s) 906
NcoI CCATGG 2 cut(s) 750, 939
NdeII GATC 3 cut(s) 349, 867, 910
NlaIII CATG 6 cut(s) 514, 533, 754, 761, 802, 943
NlaIV GGNNCC 1 cut(s) 735
NmuCI GTSAC 1 cut(s) 212
NsiI ATGCAT 1 cut(s) 506
NspI RCATGY 1 cut(s) 761
PciSI GCTCTTC 1 cut(s) 112
PcsI WCGNNNNNNNCGW 1 cut(s) 345
PctI GAATGC 1 cut(s) 329
PfeI GAWTC 3 cut(s) 170, 376, 766
PflFI GACNNNGTC 1 cut(s) 22
PkrI GCNGC 3 cut(s) 118, 347, 667
PleI GAGTC 1 cut(s) 312
PpsI GAGTC 1 cut(s) 312
Ppu21I YACGTR 1 cut(s) 217
PpuMI RGGWCCY 1 cut(s) 838
Psp124BI GAGCTC 1 cut(s) 606
Psp5II RGGWCCY 1 cut(s) 838
Psp6I CCWGG 2 cut(s) 597, 834
PspGI CCWGG 2 cut(s) 597, 834
PspN4I GGNNCC 1 cut(s) 735
PspPI GGNCC 1 cut(s) 838
PspPPI RGGWCCY 1 cut(s) 838
PstI CTGCAG 1 cut(s) 670
PstNI CAGNNNCTG 1 cut(s) 841
PsyI GACNNNGTC 1 cut(s) 22
RsaI GTAC 3 cut(s) 219, 412, 735
RsaNI GTAC 3 cut(s) 218, 411, 734
RseI CAYNNNNRTG 2 cut(s) 509, 582
SacI GAGCTC 1 cut(s) 606
SalI GTCGAC 1 cut(s) 958
SapI GCTCTTC 1 cut(s) 112
SaqAI TTAA 3 cut(s) 680, 821, 921
SatI GCNGC 3 cut(s) 117, 346, 666
Sau3AI GATC 3 cut(s) 349, 867, 910
Sau96I GGNCC 1 cut(s) 838
SchI GAGTC 1 cut(s) 313
ScrFI CCNGG 3 cut(s) 599, 836, 906
SduI GDGCHC 3 cut(s) 424, 606, 811
SfaNI GCATC 4 cut(s) 137, 252, 491, 661
SfcI CTRYAG 2 cut(s) 111, 666
SinI GGWCC 1 cut(s) 838
SmiMI CAYNNNNRTG 2 cut(s) 509, 582
SmlI CTYRAG 1 cut(s) 920
SmoI CTYRAG 1 cut(s) 920
Sse9I AATT 2 cut(s) 187, 774
SsiI CCGC 3 cut(s) 329, 771, 854
SspMI CTAG 1 cut(s) 954
SstI GAGCTC 1 cut(s) 606
StyD4I CCNGG 3 cut(s) 597, 834, 904
StyI CCWWGG 2 cut(s) 750, 939
TaaI ACNGT 5 cut(s) 44, 238, 560, 865, 992
TaiI ACGT 1 cut(s) 219
TaqI TCGA 4 cut(s) 173, 453, 499, 959
TasI AATT 2 cut(s) 187, 774
TfiI GAWTC 3 cut(s) 170, 376, 766
Tru1I TTAA 3 cut(s) 680, 821, 921
Tru9I TTAA 3 cut(s) 680, 821, 921
TscAI CASTG 3 cut(s) 241, 633, 868
TseFI GTSAC 1 cut(s) 212
TseI GCWGC 3 cut(s) 116, 345, 665
Tsp45I GTSAC 1 cut(s) 212
TspDTI ATGAA 4 cut(s) 325, 380, 566, 815
TspRI CASTG 3 cut(s) 241, 633, 868
Tth111I GACNNNGTC 1 cut(s) 22
Vha464I CTTAAG 1 cut(s) 920
VpaK11BI GGWCC 1 cut(s) 838
XapI RAATTY 1 cut(s) 187
XceI RCATGY 1 cut(s) 761
XcmI CCANNNNNNNNNTGG 2 cut(s) 757, 946
XmiI GTMKAC 1 cut(s) 959
XspI CTAG 1 cut(s) 954
Zsp2I ATGCAT 1 cut(s) 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.