MD17G1205400.v1.1

Asp/Glu/Hydantoin racemase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002475
Physical Location & Seq
Reverse (-)
24898456 .. 24901118
2663 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1205400.v1.1.491

Sequence Viewer

Length: 1281 bp
ATGGAAGCAACGTCTTTGCGCCCTTCGAACTATCATTTCTATAACTTGGGATTTGTAAATACACATAAAACCCTTTATATGACGAGGTTGAATCCAGGTCAAGCTAAAACTCCATCCCCAGTTCTCTTGCACACAGATGAAACTGGCCCGTTTCCAGAGTTTAAGAAGGGTTCTGGTTCAAGTGCAGCTTTAAGAAACACTGCTCCCAGAAAATCATTTCCGGGTCTACCAAACACAGTTGGCATAATTGGAGGGCTATCTGTCGATTCTAATGTTAAATTTATGAGAAAACTTGTCAATTGGAGTTCAAGAGATGGAGAAAGTTGCCCTCCTTTTGTTCTTTGTTCTGACCATGTTTCAAATAAGGAGCTTTTATCAATTGAGAGTTCTTTTCCTTCTGTCAGCAGTAAAAGCGATGGTCCTGAATTCGATCCTACTCCAATTGTGGAGAATTTGCTGAGTAAGAGGATTTTTCTTGAAAAATCGGGAGCTCGTTGCATTGTAATGCCTTGTCACATATCACATTCTTGGCATGATGAAATTTCTAAGGGATGTTCTGTTCCTTTTCTTCATATTGGTGAGTGTGTTGTCAAGGAGCTCAAGGAAGCAAAGTTGAAGCCGCTTGAAGCCGGAAGTCCTTTGCGGATTGGAGTGCTTGCAAGCCATGCAACTTTGACAGCAGGATTCTATCAGGAGAAACTGCAGAGCGAGGGATTTGAGGTTGTTCTGCCGGATAAAGTGACCATGGAATACTCTGTAATCCCTGCTATTGAAGCCTTAAACAGAAAGGACATAGAGGGGGCACAAAATCTCTTGAGGATCGCACTCCAGGTTCTTCTGGCGAGGGTTGTGAACTCTGTTATTCTTGCGTCCGATGATATGCGAGGTCTTCTTCACCTGGATCCTCTTCTTAAGAAATGCATTGACCCTATAGATGCCTTGGCTAGGTCAGCCATAAGCTGGGCTCAGTCTGCTAAAAAAGATGGTTCAAAGAAGAAAAAACAGAAGAAGAAAAAGGATCCGAATGCACCCAAAAGAGCGATGTCTGGTTTCATGTTCTTCTCAAATATGGAGAGAGATAACCCTGGAATTGCGTTTACTGATGTGGGGAGAGTGCTTGGAGAGAAGTGGAGAAAGATGTCTGCGGAGGAAAAGGAGCCATATGAAGCAAAGGCTCGTCAAGATAAGGAACGCTACAAGGATGATATTAGAAGCTACAAGAACCCCCAACCCATGAACATAGATTCACAAGGAGTGCAGATTGGACGAATTTCTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000785 GO:0000790 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006323 GO:0006325 GO:0006333 GO:0006334 GO:0006338 GO:0006342 GO:0006355 GO:0006357 GO:0006725 GO:0006807 GO:0006950 GO:0006996 GO:0007154 GO:0008023 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009267 GO:0009605 GO:0009607 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0009991 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0016043 GO:0016458 GO:0019219 GO:0019222 GO:0022607 GO:0030447 GO:0030466 GO:0030702 GO:0031298 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031334 GO:0031497 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032991 GO:0033036 GO:0033554 GO:0034613 GO:0034622 GO:0034641 GO:0034645 GO:0034724 GO:0034728 GO:0035101 GO:0036170 GO:0036180 GO:0040007 GO:0040029 GO:0042594 GO:0043044 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0043486 GO:0043596 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044182 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0045814 GO:0045892 GO:0045893 GO:0045898 GO:0045899 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0051641 GO:0051716 GO:0060255 GO:0060260 GO:0060261 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070727 GO:0071103 GO:0071496 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1901576 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1990141 GO:2000112 GO:2000113 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
POB3_N Rtt106 SSrecog

Protein Analysis

427

Amino Acids

47.23

Weight (kDa)

9.18

Isoelectric Point (pI)

49.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp_Glu_race PF01177 83 - 318 1.4e-25 Asp/Glu/Hydantoin racemase
HMG_box_2 PF09011 341 - 406 2.5e-12 HMG-box domain
HMG_box PF00505 344 - 407 1.3e-16 HMG (high mobility group) box
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 960
AccI GTMKAC 1 cut(s) 226
AciI CCGC 3 cut(s) 620, 643, 1145
AclWI GGATC 6 cut(s) 425, 827, 896, 909, 1013, 1026
AcsI RAATTY 5 cut(s) 278, 425, 451, 540, 1269
AfiI CCNNNNNNNGG 2 cut(s) 945, 960
AflII CTTAAG 1 cut(s) 911
AgsI TTSAA 9 cut(s) 91, 180, 309, 360, 479, 616, 626, 773, 990
AjnI CCWGG 4 cut(s) 94, 828, 897, 1084
AloI GAACNNNNNNTCC 2 cut(s) 543, 575
AluBI AGCT 7 cut(s) 104, 188, 370, 491, 598, 960, 1215
AluI AGCT 7 cut(s) 104, 188, 370, 491, 598, 960, 1215
Alw21I GWGCWC 2 cut(s) 493, 600
AlwI GGATC 6 cut(s) 425, 827, 896, 909, 1013, 1026
AoxI GGCC 1 cut(s) 145
ApeKI GCWGC 1 cut(s) 185
ApoI RAATTY 5 cut(s) 278, 425, 451, 540, 1269
AspLEI GCGC 1 cut(s) 21
AspS9I GGNCC 2 cut(s) 146, 419
AsuC2I CCSGG 1 cut(s) 222
AsuHPI GGTGA 2 cut(s) 590, 887
AsuII TTCGAA 1 cut(s) 26
AvaII GGWCC 1 cut(s) 419
BaeGI GKGCMC 1 cut(s) 805
BamHI GGATCC 2 cut(s) 901, 1018
BanII GRGCYC 3 cut(s) 493, 600, 967
BbsI GAAGAC 1 cut(s) 881
Bbv12I GWGCWC 2 cut(s) 493, 600
BbvI GCAGC 1 cut(s) 197
BccI CCATC 4 cut(s) 121, 308, 410, 977
BciT130I CCWGG 4 cut(s) 96, 830, 899, 1086
BcnI CCSGG 1 cut(s) 222
BfaI CTAG 1 cut(s) 945
BfmI CTRYAG 2 cut(s) 701, 930
BfrI CTTAAG 1 cut(s) 911
BisI GCNGC 2 cut(s) 186, 620
BlsI GCNGC 2 cut(s) 187, 621
Bme1390I CCNGG 5 cut(s) 96, 222, 830, 899, 1086
Bme18I GGWCC 1 cut(s) 419
BmgT120I GGNCC 2 cut(s) 146, 419
BmiI GGNNCC 3 cut(s) 903, 1020, 1158
BmrFI CCNGG 5 cut(s) 96, 222, 830, 899, 1086
BmrI ACTGGG 1 cut(s) 113
BmsI GCATC 1 cut(s) 925
BmuI ACTGGG 1 cut(s) 113
BpiI GAAGAC 1 cut(s) 881
BpmI CTGGAG 1 cut(s) 812
Bpu14I TTCGAA 1 cut(s) 26
BpuEI CTTGAG 2 cut(s) 584, 835
BpuMI CCSGG 1 cut(s) 222
BsaJI CCNNGG 3 cut(s) 744, 939, 1084
Bsc4I CCNNNNNNNGG 2 cut(s) 945, 960
Bse1I ACTGG 2 cut(s) 119, 148
BseBI CCWGG 4 cut(s) 96, 830, 899, 1086
BseDI CCNNGG 3 cut(s) 744, 939, 1084
BseGI GGATG 3 cut(s) 113, 557, 1207
BseLI CCNNNNNNNGG 2 cut(s) 945, 960
BseMII CTCAG 2 cut(s) 449, 980
BseNI ACTGG 2 cut(s) 119, 148
BseSI GKGCMC 1 cut(s) 805
BseXI GCAGC 1 cut(s) 197
BseYI CCCAGC 1 cut(s) 960
BsgI GTGCAG 2 cut(s) 204, 1277
BshFI GGCC 1 cut(s) 147
BsiHKAI GWGCWC 2 cut(s) 493, 600
BsiSI CCGG 3 cut(s) 221, 630, 731
BslI CCNNNNNNNGG 2 cut(s) 945, 960
BsmI GAATGC 1 cut(s) 1030
BsnI GGCC 1 cut(s) 147
Bsp119I TTCGAA 1 cut(s) 26
Bsp1286I GDGCHC 4 cut(s) 493, 600, 805, 967
Bsp143I GATC 4 cut(s) 430, 819, 901, 1018
Bsp19I CCATGG 1 cut(s) 744
BspACI CCGC 3 cut(s) 620, 643, 1145
BspANI GGCC 1 cut(s) 147
BspCNI CTCAG 2 cut(s) 450, 979
BspLI GGNNCC 3 cut(s) 903, 1020, 1158
BspMAI CTGCAG 1 cut(s) 705
BspPI GGATC 6 cut(s) 425, 827, 896, 909, 1013, 1026
BspT104I TTCGAA 1 cut(s) 26
BspTI CTTAAG 1 cut(s) 911
BsrI ACTGG 2 cut(s) 119, 148
BssECI CCNNGG 3 cut(s) 744, 939, 1084
BssMI GATC 4 cut(s) 430, 819, 901, 1018
BssT1I CCWWGG 2 cut(s) 744, 939
Bst2UI CCWGG 4 cut(s) 96, 830, 899, 1086
Bst4CI ACNGT 1 cut(s) 238
Bst6I CTCTTC 1 cut(s) 912
BstAFI CTTAAG 1 cut(s) 911
BstAPI GCANNNNNTGC 1 cut(s) 665
BstBI TTCGAA 1 cut(s) 26
BstC8I GCNNGC 2 cut(s) 657, 661
BstDEI CTNAG 3 cut(s) 458, 546, 966
BstDSI CCRYGG 1 cut(s) 744
BstENI CCTNNNNNAGG 1 cut(s) 943
BstF5I GGATG 3 cut(s) 113, 557, 1207
BstHHI GCGC 1 cut(s) 21
BstKTI GATC 4 cut(s) 433, 822, 904, 1021
BstMBI GATC 4 cut(s) 430, 819, 901, 1018
BstMWI GCNNNNNNNGC 5 cut(s) 411, 665, 773, 950, 971
BstNI CCWGG 4 cut(s) 96, 830, 899, 1086
BstSCI CCNGG 5 cut(s) 94, 220, 828, 897, 1084
BstSFI CTRYAG 2 cut(s) 701, 930
BstSLI GKGCMC 1 cut(s) 805
BstV1I GCAGC 1 cut(s) 197
BstV2I GAAGAC 1 cut(s) 881
BstX2I RGATCY 2 cut(s) 901, 1018
BstYI RGATCY 2 cut(s) 901, 1018
BsuRI GGCC 1 cut(s) 147
BtgI CCRYGG 1 cut(s) 744
BtgZI GCGATG 2 cut(s) 429, 1055
BtsCI GGATG 3 cut(s) 113, 557, 1207
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 1 cut(s) 198
Cac8I GCNNGC 2 cut(s) 657, 661
CfoI GCGC 1 cut(s) 21
Cfr13I GGNCC 2 cut(s) 146, 419
CseI GACGC 1 cut(s) 858
CviAII CATG 6 cut(s) 353, 533, 665, 745, 1054, 1234
DdeI CTNAG 3 cut(s) 458, 546, 966
DpnI GATC 4 cut(s) 432, 821, 903, 1020
DpnII GATC 4 cut(s) 430, 819, 901, 1018
Eam1104I CTCTTC 1 cut(s) 912
EarI CTCTTC 1 cut(s) 912
Ecl136II GAGCTC 2 cut(s) 491, 598
Eco130I CCWWGG 2 cut(s) 744, 939
Eco24I GRGCYC 3 cut(s) 493, 600, 967
Eco47I GGWCC 1 cut(s) 419
Eco53kI GAGCTC 2 cut(s) 491, 598
EcoICRI GAGCTC 2 cut(s) 491, 598
EcoNI CCTNNNNNAGG 1 cut(s) 943
EcoRI GAATTC 1 cut(s) 425
EcoRII CCWGG 4 cut(s) 94, 828, 897, 1084
EcoT14I CCWWGG 2 cut(s) 744, 939
EcoT22I ATGCAT 1 cut(s) 923
EcoT38I GRGCYC 3 cut(s) 493, 600, 967
ErhI CCWWGG 2 cut(s) 744, 939
FaeI CATG 6 cut(s) 356, 536, 668, 748, 1057, 1237
FalI AAGNNNNNCTT 2 cut(s) 172, 204
FatI CATG 6 cut(s) 352, 532, 664, 744, 1053, 1233
FauNDI CATATG 1 cut(s) 1162
FblI GTMKAC 1 cut(s) 226
Fnu4HI GCNGC 2 cut(s) 186, 620
FokI GGATG 3 cut(s) 100, 564, 1214
FriOI GRGCYC 3 cut(s) 493, 600, 967
Fsp4HI GCNGC 2 cut(s) 186, 620
FspBI CTAG 1 cut(s) 945
GlaI GCGC 1 cut(s) 20
GluI GCNGC 2 cut(s) 186, 620
GsaI CCCAGC 1 cut(s) 964
GsuI CTGGAG 1 cut(s) 812
HaeIII GGCC 1 cut(s) 147
HapII CCGG 3 cut(s) 221, 630, 731
HgaI GACGC 1 cut(s) 858
HhaI GCGC 1 cut(s) 21
Hin1II CATG 6 cut(s) 356, 536, 668, 748, 1057, 1237
Hin6I GCGC 1 cut(s) 19
HinP1I GCGC 1 cut(s) 19
HinfI GANTC 4 cut(s) 91, 266, 684, 1244
HpaII CCGG 3 cut(s) 221, 630, 731
HphI GGTGA 2 cut(s) 590, 887
Hpy166II GTNNAC 3 cut(s) 227, 853, 1098
Hpy188I TCNGA 3 cut(s) 349, 874, 1023
Hpy188III TCNNGA 8 cut(s) 155, 309, 422, 476, 486, 692, 814, 1181
Hpy8I GTNNAC 3 cut(s) 227, 853, 1098
HpyAV CCTTC 3 cut(s) 33, 160, 405
HpyCH4III ACNGT 1 cut(s) 238
HpyCH4IV ACGT 1 cut(s) 11
HpyCH4V TGCA 9 cut(s) 130, 185, 498, 659, 668, 703, 921, 1028, 1258
HpyF10VI GCNNNNNNNGC 5 cut(s) 411, 665, 773, 950, 971
HpyF3I CTNAG 3 cut(s) 458, 546, 966
HpySE526I ACGT 1 cut(s) 11
Hsp92II CATG 6 cut(s) 356, 536, 668, 748, 1057, 1237
HspAI GCGC 1 cut(s) 19
Kzo9I GATC 4 cut(s) 430, 819, 901, 1018
LmnI GCTCC 5 cut(s) 208, 367, 488, 595, 1156
Lsp1109I GCAGC 1 cut(s) 197
LweI GCATC 1 cut(s) 925
MaeI CTAG 1 cut(s) 945
MaeII ACGT 1 cut(s) 11
MaeIII GTNAC 2 cut(s) 512, 739
MalI GATC 4 cut(s) 432, 821, 903, 1020
MboI GATC 4 cut(s) 430, 819, 901, 1018
MboII GAAGA 9 cut(s) 560, 827, 881, 884, 899, 1006, 1018, 1021, 1051
MfeI CAATTG 3 cut(s) 298, 378, 441
MflI RGATCY 2 cut(s) 901, 1018
MhlI GDGCHC 4 cut(s) 493, 600, 805, 967
Mph1103I ATGCAT 1 cut(s) 923
MseI TTAA 5 cut(s) 162, 191, 276, 779, 912
MslI CAYNNNNRTG 3 cut(s) 135, 503, 576
MspCI CTTAAG 1 cut(s) 911
MspI CCGG 3 cut(s) 221, 630, 731
MspR9I CCNGG 5 cut(s) 96, 222, 830, 899, 1086
MunI CAATTG 3 cut(s) 298, 378, 441
Mva1269I GAATGC 1 cut(s) 1030
MvaI CCWGG 4 cut(s) 96, 830, 899, 1086
MwoI GCNNNNNNNGC 5 cut(s) 411, 665, 773, 950, 971
NciI CCSGG 1 cut(s) 222
NcoI CCATGG 1 cut(s) 744
NdeI CATATG 1 cut(s) 1162
NdeII GATC 4 cut(s) 430, 819, 901, 1018
NlaIII CATG 6 cut(s) 356, 536, 668, 748, 1057, 1237
NlaIV GGNNCC 3 cut(s) 903, 1020, 1158
NmuCI GTSAC 2 cut(s) 512, 739
NsiI ATGCAT 1 cut(s) 923
NspV TTCGAA 1 cut(s) 26
PctI GAATGC 1 cut(s) 1030
PfeI GAWTC 4 cut(s) 91, 266, 684, 1244
PflMI CCANNNNNTGG 1 cut(s) 960
PkrI GCNGC 2 cut(s) 187, 621
Psp124BI GAGCTC 2 cut(s) 493, 600
Psp6I CCWGG 4 cut(s) 94, 828, 897, 1084
PspFI CCCAGC 1 cut(s) 960
PspGI CCWGG 4 cut(s) 94, 828, 897, 1084
PspN4I GGNNCC 3 cut(s) 903, 1020, 1158
PspPI GGNCC 2 cut(s) 146, 419
PstI CTGCAG 1 cut(s) 705
PsuI RGATCY 2 cut(s) 901, 1018
RseI CAYNNNNRTG 3 cut(s) 135, 503, 576
SacI GAGCTC 2 cut(s) 493, 600
SaqAI TTAA 5 cut(s) 162, 191, 276, 779, 912
SatI GCNGC 2 cut(s) 186, 620
Sau3AI GATC 4 cut(s) 430, 819, 901, 1018
Sau96I GGNCC 2 cut(s) 146, 419
ScrFI CCNGG 5 cut(s) 96, 222, 830, 899, 1086
SduI GDGCHC 4 cut(s) 493, 600, 805, 967
SfaNI GCATC 1 cut(s) 925
SfcI CTRYAG 2 cut(s) 701, 930
SfuI TTCGAA 1 cut(s) 26
SinI GGWCC 1 cut(s) 419
SmiMI CAYNNNNRTG 3 cut(s) 135, 503, 576
SmlI CTYRAG 3 cut(s) 599, 814, 911
SmoI CTYRAG 3 cut(s) 599, 814, 911
SsiI CCGC 3 cut(s) 620, 643, 1145
SspMI CTAG 1 cut(s) 945
SstI GAGCTC 2 cut(s) 493, 600
StyD4I CCNGG 5 cut(s) 94, 220, 828, 897, 1084
StyI CCWWGG 2 cut(s) 744, 939
TaaI ACNGT 1 cut(s) 238
TaiI ACGT 1 cut(s) 14
TaqI TCGA 3 cut(s) 26, 264, 429
TauI GCSGC 1 cut(s) 622
TfiI GAWTC 4 cut(s) 91, 266, 684, 1244
Tru1I TTAA 5 cut(s) 162, 191, 276, 779, 912
Tru9I TTAA 5 cut(s) 162, 191, 276, 779, 912
TscAI CASTG 1 cut(s) 205
TseFI GTSAC 2 cut(s) 512, 739
TseI GCWGC 1 cut(s) 185
Tsp45I GTSAC 2 cut(s) 512, 739
TspDTI ATGAA 6 cut(s) 153, 552, 560, 1042, 1179, 1250
TspRI CASTG 1 cut(s) 205
Van91I CCANNNNNTGG 1 cut(s) 960
Vha464I CTTAAG 1 cut(s) 911
VpaK11BI GGWCC 1 cut(s) 419
XagI CCTNNNNNAGG 1 cut(s) 943
XapI RAATTY 5 cut(s) 278, 425, 451, 540, 1269
XmiI GTMKAC 1 cut(s) 226
XspI CTAG 1 cut(s) 945
Zsp2I ATGCAT 1 cut(s) 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.