MD02G1035500.v1.1

Asp/Glu/Hydantoin racemase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0002475
Physical Location & Seq
Forward (+)
2705268 .. 2706223
956 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1035500.v1.1.491

Sequence Viewer

Length: 912 bp
ATGGTTGATGCAGGCATGACAATGTCCTTCCACACACAAAACCGTCCACCTCTTCTTTTCGGACTTTTGACCAAAAATAGGACACAATACAGAACCAAATCAAGCTCTTTTGTAGCCGCAGTGCAAGTATCTTCAGTTCTGCAAACCAATGAGAGCGGAAATCTACCTGAATCGAAGAAAGTTCGGAATTCTGGCTTACCTCTAACAAGATGCCACGTACTGAGCCAACCAAACACCGTCGGCATCATTGGAGGGGTGTCTGTTTATTCAAGCCTCCTTTTCTTGGAGAAGCTTGTGTGGTGGAGTCTAAAAGATGGAGGAGAATGCCCGCCTTTCATCGTGTGCAGCGATCCAACGTTGTATAAGGAGCTTCCGATTCGCAGTTTGTTGCATTCACTCAAACGAAGTACTAGTAGTGCTCAAATCCTATCAAATAACTGGCCTGTCATCGAGAGCTTGTGTCGCAAGAGGGCGTTCCTCGAGCACTCTGGCGCTCGATGCATAGTCATGCCGTGTCACCTGTCTCATGCGTGGCACGACCAGATATCTGAGGACTGTTCTCTGCGCTTCCTTCATATCGGTGAGTGTGTTGCCCGGGAGCTCAGGGAAGCAAAGTTGAAGCCACTGGAAAAAGGAAGTAATGTAAGGATTGGAGTGCTTGCTGCAGATGCAACTTTAACGGCTGGTTTTTACCAAGAAAAGCTACAAAGTCAGGTACACATTCATCCTAATTTTGTTTTTCTTGCTAATTTATCAAATTATGTGAAAACCATGAAGATAACAATTCCGATTTTCTTCGAAAACATTACTTGTATTTTAAGTCACCCGCGAAAAACATATTTCGGTGCAATGGCAATGGACAGGGGTTTGATGTTGTGGTACCTGACAAGCAAACCATGGAGCATTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asp_Glu_race

Protein Analysis

304

Amino Acids

34.12

Weight (kDa)

9.38

Isoelectric Point (pI)

42.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp_Glu_race PF01177 83 - 238 1.3e-10 Asp/Glu/Hydantoin racemase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 879
AccB1I GGYRCC 1 cut(s) 879
AccBSI CCGCTC 1 cut(s) 156
AccII CGCG 1 cut(s) 829
AciI CCGC 4 cut(s) 117, 156, 329, 827
AclI AACGTT 1 cut(s) 356
AclWI GGATC 1 cut(s) 344
AcsI RAATTY 1 cut(s) 187
AcuI CTGAAG 1 cut(s) 117
AfaI GTAC 4 cut(s) 219, 409, 717, 881
AfiI CCNNNNNNNGG 2 cut(s) 78, 283
AgsI TTSAA 2 cut(s) 270, 619
AhlI ACTAGT 1 cut(s) 410
AluBI AGCT 6 cut(s) 105, 292, 370, 456, 601, 703
AluI AGCT 6 cut(s) 105, 292, 370, 456, 601, 703
Alw21I GWGCWC 3 cut(s) 421, 486, 603
Alw26I GTCTC 1 cut(s) 528
AlwI GGATC 1 cut(s) 344
Ama87I CYCGRG 2 cut(s) 479, 594
AoxI GGCC 1 cut(s) 440
ApeKI GCWGC 2 cut(s) 345, 662
ApoI RAATTY 1 cut(s) 187
Asp718I GGTACC 1 cut(s) 879
AspLEI GCGC 2 cut(s) 494, 567
AsuC2I CCSGG 2 cut(s) 595, 596
AsuHPI GGTGA 3 cut(s) 509, 593, 815
AsuII TTCGAA 1 cut(s) 798
AvaI CYCGRG 2 cut(s) 479, 594
BanI GGYRCC 1 cut(s) 879
BanII GRGCYC 1 cut(s) 603
Bbv12I GWGCWC 3 cut(s) 421, 486, 603
BbvI GCAGC 2 cut(s) 357, 649
BccI CCATC 1 cut(s) 308
BceAI ACGGC 2 cut(s) 496, 696
BcnI CCSGG 2 cut(s) 595, 596
BcoDI GTCTC 1 cut(s) 528
BcuI ACTAGT 1 cut(s) 410
BfaI CTAG 1 cut(s) 411
BfmI CTRYAG 1 cut(s) 663
BfoI RGCGCY 1 cut(s) 495
BisI GCNGC 3 cut(s) 117, 346, 663
BlsI GCNGC 3 cut(s) 118, 347, 664
BmcAI AGTACT 1 cut(s) 409
Bme1390I CCNGG 2 cut(s) 595, 596
BmeT110I CYCGRG 2 cut(s) 479, 594
BmiI GGNNCC 1 cut(s) 881
BmrFI CCNGG 2 cut(s) 595, 596
BmsI GCATC 4 cut(s) 200, 252, 488, 658
Bpu10I CCTNAGC 1 cut(s) 602
Bpu14I TTCGAA 1 cut(s) 798
BpuMI CCSGG 2 cut(s) 595, 596
BsaAI YACGTR 1 cut(s) 217
BsaJI CCNNGG 2 cut(s) 594, 896
Bsc4I CCNNNNNNNGG 2 cut(s) 78, 283
Bse1I ACTGG 2 cut(s) 443, 630
Bse3DI GCAATG 2 cut(s) 855, 861
BseDI CCNNGG 2 cut(s) 594, 896
BseGI GGATG 1 cut(s) 724
BseLI CCNNNNNNNGG 2 cut(s) 78, 283
BseMI GCAATG 2 cut(s) 855, 861
BseMII CTCAG 3 cut(s) 212, 540, 616
BseNI ACTGG 2 cut(s) 443, 630
BseRI GAGGAG 1 cut(s) 333
BseXI GCAGC 2 cut(s) 357, 649
BsgI GTGCAG 1 cut(s) 364
Bsh1236I CGCG 1 cut(s) 829
BshFI GGCC 1 cut(s) 442
BshNI GGYRCC 1 cut(s) 879
BsiHKAI GWGCWC 3 cut(s) 421, 486, 603
BsiHKCI CYCGRG 2 cut(s) 479, 594
BsiSI CCGG 1 cut(s) 595
BslI CCNNNNNNNGG 2 cut(s) 78, 283
BsmAI GTCTC 1 cut(s) 528
BsmI GAATGC 2 cut(s) 329, 391
BsnI GGCC 1 cut(s) 442
BsoBI CYCGRG 2 cut(s) 479, 594
Bsp119I TTCGAA 1 cut(s) 798
Bsp1286I GDGCHC 3 cut(s) 421, 486, 603
Bsp143I GATC 1 cut(s) 349
Bsp19I CCATGG 1 cut(s) 896
BspACI CCGC 4 cut(s) 117, 156, 329, 827
BspANI GGCC 1 cut(s) 442
BspCNI CTCAG 3 cut(s) 213, 541, 615
BspFNI CGCG 1 cut(s) 829
BspLI GGNNCC 1 cut(s) 881
BspMAI CTGCAG 1 cut(s) 667
BspPI GGATC 1 cut(s) 344
BspT104I TTCGAA 1 cut(s) 798
BspT107I GGYRCC 1 cut(s) 879
BsrBI CCGCTC 1 cut(s) 156
BsrDI GCAATG 2 cut(s) 855, 861
BsrI ACTGG 2 cut(s) 443, 630
BssECI CCNNGG 2 cut(s) 594, 896
BssMI GATC 1 cut(s) 349
BssT1I CCWWGG 1 cut(s) 896
Bst4CI ACNGT 3 cut(s) 44, 238, 557
Bst6I CTCTTC 1 cut(s) 57
BstBAI YACGTR 1 cut(s) 217
BstBI TTCGAA 1 cut(s) 798
BstC8I GCNNGC 3 cut(s) 13, 329, 660
BstDEI CTNAG 3 cut(s) 221, 549, 602
BstDSI CCRYGG 1 cut(s) 896
BstF5I GGATG 1 cut(s) 724
BstFNI CGCG 1 cut(s) 829
BstH2I RGCGCY 1 cut(s) 495
BstHHI GCGC 2 cut(s) 494, 567
BstKTI GATC 1 cut(s) 352
BstMAI GTCTC 1 cut(s) 528
BstMBI GATC 1 cut(s) 349
BstMWI GCNNNNNNNGC 3 cut(s) 462, 498, 668
BstSCI CCNGG 2 cut(s) 593, 594
BstSFI CTRYAG 1 cut(s) 663
BstUI CGCG 1 cut(s) 829
BstV1I GCAGC 2 cut(s) 357, 649
BsuRI GGCC 1 cut(s) 442
BtgI CCRYGG 1 cut(s) 896
BtsCI GGATG 1 cut(s) 724
BtsI GCAGTG 1 cut(s) 126
BtsIMutI CAGTG 2 cut(s) 126, 623
Cac8I GCNNGC 3 cut(s) 13, 329, 660
CfoI GCGC 2 cut(s) 494, 567
Cfr9I CCCGGG 1 cut(s) 594
Csp6I GTAC 4 cut(s) 218, 408, 716, 880
CviAII CATG 5 cut(s) 16, 508, 527, 772, 897
CviQI GTAC 4 cut(s) 218, 408, 716, 880
DdeI CTNAG 3 cut(s) 221, 549, 602
DpnI GATC 1 cut(s) 351
DpnII GATC 1 cut(s) 349
Eam1104I CTCTTC 1 cut(s) 57
EarI CTCTTC 1 cut(s) 57
Ecl136II GAGCTC 1 cut(s) 601
Eco130I CCWWGG 1 cut(s) 896
Eco24I GRGCYC 1 cut(s) 603
Eco32I GATATC 1 cut(s) 546
Eco53kI GAGCTC 1 cut(s) 601
Eco57I CTGAAG 1 cut(s) 117
Eco88I CYCGRG 2 cut(s) 479, 594
EcoICRI GAGCTC 1 cut(s) 601
EcoRI GAATTC 1 cut(s) 187
EcoRV GATATC 1 cut(s) 546
EcoT14I CCWWGG 1 cut(s) 896
EcoT22I ATGCAT 1 cut(s) 503
EcoT38I GRGCYC 1 cut(s) 603
ErhI CCWWGG 1 cut(s) 896
FaeI CATG 5 cut(s) 19, 511, 530, 775, 900
FatI CATG 5 cut(s) 15, 507, 526, 771, 896
FauI CCCGC 2 cut(s) 336, 834
Fnu4HI GCNGC 3 cut(s) 117, 346, 663
FokI GGATG 1 cut(s) 711
FriOI GRGCYC 1 cut(s) 603
Fsp4HI GCNGC 3 cut(s) 117, 346, 663
FspBI CTAG 1 cut(s) 411
GlaI GCGC 2 cut(s) 493, 566
GluI GCNGC 3 cut(s) 117, 346, 663
HaeII RGCGCY 1 cut(s) 495
HaeIII GGCC 1 cut(s) 442
HapII CCGG 1 cut(s) 595
HhaI GCGC 2 cut(s) 494, 567
Hin1II CATG 5 cut(s) 19, 511, 530, 775, 900
Hin6I GCGC 2 cut(s) 492, 565
HinP1I GCGC 2 cut(s) 492, 565
HindIII AAGCTT 1 cut(s) 290
HinfI GANTC 3 cut(s) 170, 304, 376
HpaII CCGG 1 cut(s) 595
HphI GGTGA 3 cut(s) 509, 593, 815
Hpy166II GTNNAC 2 cut(s) 47, 718
Hpy188I TCNGA 5 cut(s) 62, 186, 375, 550, 789
Hpy188III TCNNGA 1 cut(s) 451
Hpy8I GTNNAC 2 cut(s) 47, 718
Hpy99I CGWCG 1 cut(s) 242
HpyAV CCTTC 2 cut(s) 37, 581
HpyCH4III ACNGT 3 cut(s) 44, 238, 557
HpyCH4IV ACGT 2 cut(s) 216, 356
HpyCH4V TGCA 9 cut(s) 11, 124, 142, 345, 391, 501, 665, 671, 848
HpyF10VI GCNNNNNNNGC 3 cut(s) 462, 498, 668
HpyF3I CTNAG 3 cut(s) 221, 549, 602
HpySE526I ACGT 2 cut(s) 216, 356
Hsp92II CATG 5 cut(s) 19, 511, 530, 775, 900
HspAI GCGC 2 cut(s) 492, 565
KpnI GGTACC 1 cut(s) 883
Kzo9I GATC 1 cut(s) 349
LmnI GCTCC 3 cut(s) 367, 598, 900
Lsp1109I GCAGC 2 cut(s) 357, 649
LweI GCATC 4 cut(s) 200, 252, 488, 658
MaeI CTAG 1 cut(s) 411
MaeII ACGT 2 cut(s) 216, 356
MaeIII GTNAC 2 cut(s) 515, 821
MalI GATC 1 cut(s) 351
MbiI CCGCTC 1 cut(s) 156
MboI GATC 1 cut(s) 349
MboII GAAGA 5 cut(s) 44, 123, 187, 787, 787
MhlI GDGCHC 3 cut(s) 421, 486, 603
MluCI AATT 5 cut(s) 187, 730, 748, 757, 783
MlyI GAGTC 1 cut(s) 313
MmeI TCCRAC 1 cut(s) 377
MnlI CCTC 8 cut(s) 60, 210, 245, 284, 311, 462, 488, 544
Mph1103I ATGCAT 1 cut(s) 503
MseI TTAA 2 cut(s) 677, 818
MslI CAYNNNNRTG 3 cut(s) 20, 506, 579
MspI CCGG 1 cut(s) 595
MspR9I CCNGG 2 cut(s) 595, 596
Mva1269I GAATGC 2 cut(s) 329, 391
MvnI CGCG 1 cut(s) 829
MwoI GCNNNNNNNGC 3 cut(s) 462, 498, 668
NciI CCSGG 2 cut(s) 595, 596
NcoI CCATGG 1 cut(s) 896
NdeII GATC 1 cut(s) 349
NlaIII CATG 5 cut(s) 19, 511, 530, 775, 900
NlaIV GGNNCC 1 cut(s) 881
NmuCI GTSAC 2 cut(s) 515, 821
NsiI ATGCAT 1 cut(s) 503
NspV TTCGAA 1 cut(s) 798
PaeR7I CTCGAG 1 cut(s) 479
PcsI WCGNNNNNNNCGW 1 cut(s) 345
PctI GAATGC 2 cut(s) 329, 391
PfeI GAWTC 2 cut(s) 170, 376
PflFI GACNNNGTC 1 cut(s) 22
PkrI GCNGC 3 cut(s) 118, 347, 664
PleI GAGTC 1 cut(s) 312
PpsI GAGTC 1 cut(s) 312
Ppu21I YACGTR 1 cut(s) 217
Psp124BI GAGCTC 1 cut(s) 603
Psp1406I AACGTT 1 cut(s) 356
PspN4I GGNNCC 1 cut(s) 881
PspXI VCTCGAGB 1 cut(s) 479
PsrI GAACNNNNNNTAC 2 cut(s) 120, 152
PstI CTGCAG 1 cut(s) 667
PsyI GACNNNGTC 1 cut(s) 22
RsaI GTAC 4 cut(s) 219, 409, 717, 881
RsaNI GTAC 4 cut(s) 218, 408, 716, 880
RseI CAYNNNNRTG 3 cut(s) 20, 506, 579
SacI GAGCTC 1 cut(s) 603
SaqAI TTAA 2 cut(s) 677, 818
SatI GCNGC 3 cut(s) 117, 346, 663
Sau3AI GATC 1 cut(s) 349
ScaI AGTACT 1 cut(s) 409
SchI GAGTC 1 cut(s) 313
ScrFI CCNGG 2 cut(s) 595, 596
SduI GDGCHC 3 cut(s) 421, 486, 603
SfaNI GCATC 4 cut(s) 200, 252, 488, 658
SfcI CTRYAG 1 cut(s) 663
Sfr274I CTCGAG 1 cut(s) 479
SfuI TTCGAA 1 cut(s) 798
SlaI CTCGAG 1 cut(s) 479
SmaI CCCGGG 1 cut(s) 596
SmiMI CAYNNNNRTG 3 cut(s) 20, 506, 579
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
SpeI ACTAGT 1 cut(s) 410
Sse9I AATT 5 cut(s) 187, 730, 748, 757, 783
SsiI CCGC 4 cut(s) 117, 156, 329, 827
SspMI CTAG 1 cut(s) 411
SstI GAGCTC 1 cut(s) 603
StyD4I CCNGG 2 cut(s) 593, 594
StyI CCWWGG 1 cut(s) 896
TaaI ACNGT 3 cut(s) 44, 238, 557
TaiI ACGT 2 cut(s) 219, 359
TaqI TCGA 5 cut(s) 173, 450, 480, 496, 798
TasI AATT 5 cut(s) 187, 730, 748, 757, 783
TatI WGTACW 1 cut(s) 407
TauI GCSGC 1 cut(s) 119
TfiI GAWTC 2 cut(s) 170, 376
Tru1I TTAA 2 cut(s) 677, 818
Tru9I TTAA 2 cut(s) 677, 818
TscAI CASTG 2 cut(s) 126, 630
TseFI GTSAC 2 cut(s) 515, 821
TseI GCWGC 2 cut(s) 345, 662
Tsp45I GTSAC 2 cut(s) 515, 821
TspDTI ATGAA 4 cut(s) 325, 563, 713, 788
TspMI CCCGGG 1 cut(s) 594
TspRI CASTG 2 cut(s) 126, 630
Tth111I GACNNNGTC 1 cut(s) 22
XapI RAATTY 1 cut(s) 187
XcmI CCANNNNNNNNNTGG 1 cut(s) 903
XhoI CTCGAG 1 cut(s) 479
XmaI CCCGGG 1 cut(s) 594
XspI CTAG 1 cut(s) 411
ZrmI AGTACT 1 cut(s) 409
Zsp2I ATGCAT 1 cut(s) 503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.