RchiOBHm_Chr2g0089121

Asp/Glu/Hydantoin racemase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002475
Physical Location & Seq
Forward (+)
3420615 .. 3422508
1894 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46442

Sequence Viewer

Length: 1050 bp
ATGCTAGTAGAAGTAGAAATATGCTCCTGGGTTCTTGATTGGTGCAGGGCTAGCCTGACAATGTCCTTCCACACCCAAAATCGTCCTACTCATCTTCTCGGGGTTCTAAGCGAAAACCGAACCCAAAACAGAACCAAATCAAGCCCATCTGTAGCTGTACAAGTATCCTCAGTCCTACAAACTAATGGAAAAAGTGGAAATGGGAAGGGAAATCTATCTGATCAGTCTAAGAAAGGCATGGCTCTGAAAAAGTGTCAAGTCCCCAATTTTGTACTAAGCCAACCAAACACTGTGGGCATCATTGGAGGAGTGTCTGTATATTCTAGCCTGCTTTTCTTGGAGAAGCTTGTTTGGTGGAGTCTGAAAGATGGAGGAGAATGCCCACCTTTTCTTGTGTGCAGTGATACAACACAATATAAAGAGCTTCCGATTCGCAGTTTGTTTCAATCACTCAAGAGAAAAGCTACTGAGATGAAATATTCAAGCAGCAATTGGGCCATCATTGACAGCTTGTGTCAAAAAAGGGCATTTCTCGAGAACTCTGGAGCTCGATGCATAGTGATGCCCTGCCATCTGTCTCATGCTTGGCATGAACAGATTTCTGAGGACTGTTCTTTGAGTTTCCTTCATGTTGGTGAGTGTGTTGCTAGAGAGCTCAGAGAAGCGAAGATGAGGCCACTCGAAACTGGGAGTAATGTGAGAATTGGGGTGCTTGCTGCTGATGCAACTTTAACTGCTGGTTTTTACCAGCAGAAGCTGAGAAATCAGGACTTTGAGGTGGTTGTACCAGACAAAGCGACGATGGAACATGTTGTGACTCCTGCAATCGAGGCATTGAAGAGAAGAGACCTGGAAGGGGCACGAAATCTGTTAAGAATCGCGGTGCAGATTCTGTTGGTGAGGGGTGTGAACATCGTCATCATCGCTTCTGATGAATTGCAGGGTGTTCTGCCTCATGATGATCCTCTTCTTAAGAAATGTATTGATCCCATGGATGCTTTGGCCAGGTCAACCATAAAATGGGCAAAGGCCACAGGAAATGGTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asp_Glu_race

Protein Analysis

349

Amino Acids

38.56

Weight (kDa)

8.82

Isoelectric Point (pI)

37.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp_Glu_race PF01177 161 - 337 1.1e-23 Asp/Glu/Hydantoin racemase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1020
AccII CGCG 1 cut(s) 881
AciI CCGC 1 cut(s) 881
AclWI GGATC 2 cut(s) 956, 980
AcoI YGGCCR 1 cut(s) 1002
AfaI GTAC 3 cut(s) 159, 273, 786
AfiI CCNNNNNNNGG 2 cut(s) 856, 1020
AflII CTTAAG 1 cut(s) 971
AflIII ACRYGT 1 cut(s) 808
AgsI TTSAA 3 cut(s) 446, 483, 838
AjnI CCWGG 3 cut(s) 26, 849, 1004
AluBI AGCT 8 cut(s) 155, 346, 424, 464, 510, 548, 655, 757
AluI AGCT 8 cut(s) 155, 346, 424, 464, 510, 548, 655, 757
Alw21I GWGCWC 2 cut(s) 550, 657
Alw26I GTCTC 2 cut(s) 582, 840
AlwI GGATC 2 cut(s) 956, 980
AlwNI CAGNNNCTG 2 cut(s) 757, 892
Ama87I CYCGRG 2 cut(s) 98, 533
AoxI GGCC 4 cut(s) 495, 674, 1002, 1029
ApeKI GCWGC 2 cut(s) 486, 716
AspS9I GGNCC 1 cut(s) 495
AsuHPI GGTGA 2 cut(s) 647, 910
AsuNHI GCTAGC 1 cut(s) 50
AvaI CYCGRG 2 cut(s) 98, 533
BaeGI GKGCMC 1 cut(s) 862
BalI TGGCCA 1 cut(s) 1004
BanII GRGCYC 2 cut(s) 550, 657
Bbv12I GWGCWC 2 cut(s) 550, 657
BbvI GCAGC 2 cut(s) 498, 703
BccI CCATC 5 cut(s) 154, 362, 506, 579, 796
BciT130I CCWGG 3 cut(s) 28, 851, 1006
BciVI GTATCC 1 cut(s) 175
BclI TGATCA 1 cut(s) 220
BcoDI GTCTC 2 cut(s) 582, 840
BfaI CTAG 4 cut(s) 5, 51, 324, 648
BfmI CTRYAG 1 cut(s) 150
BfrI CTTAAG 1 cut(s) 971
BfuI GTATCC 1 cut(s) 175
BisI GCNGC 2 cut(s) 487, 717
BlsI GCNGC 2 cut(s) 488, 718
Bme1390I CCNGG 3 cut(s) 28, 851, 1006
BmeT110I CYCGRG 2 cut(s) 98, 533
BmgT120I GGNCC 1 cut(s) 495
BmrFI CCNGG 3 cut(s) 28, 851, 1006
BmrI ACTGGG 1 cut(s) 696
BmsI GCATC 5 cut(s) 306, 542, 552, 712, 985
BmtI GCTAGC 1 cut(s) 54
BmuI ACTGGG 1 cut(s) 696
BpmI CTGGAG 1 cut(s) 564
BpuEI CTTGAG 1 cut(s) 437
BsaI GGTCTC 1 cut(s) 840
BsaJI CCNNGG 2 cut(s) 27, 990
BsaXI ACNNNNNCTCC 2 cut(s) 300, 330
Bsc4I CCNNNNNNNGG 2 cut(s) 856, 1020
Bse1I ACTGG 1 cut(s) 691
BseBI CCWGG 3 cut(s) 28, 851, 1006
BseDI CCNNGG 2 cut(s) 27, 990
BseGI GGATG 1 cut(s) 1000
BseLI CCNNNNNNNGG 2 cut(s) 856, 1020
BseMII CTCAG 5 cut(s) 183, 459, 594, 670, 749
BseNI ACTGG 1 cut(s) 691
BseRI GAGGAG 2 cut(s) 321, 387
BseSI GKGCMC 1 cut(s) 862
BseXI GCAGC 2 cut(s) 498, 703
BsgI GTGCAG 3 cut(s) 64, 418, 905
Bsh1236I CGCG 1 cut(s) 881
BshFI GGCC 4 cut(s) 497, 676, 1004, 1031
BsiHKAI GWGCWC 2 cut(s) 550, 657
BsiHKCI CYCGRG 2 cut(s) 98, 533
BslFI GGGAC 1 cut(s) 245
BslI CCNNNNNNNGG 2 cut(s) 856, 1020
BsmAI GTCTC 2 cut(s) 582, 840
BsmFI GGGAC 1 cut(s) 245
BsmI GAATGC 1 cut(s) 383
BsnI GGCC 4 cut(s) 497, 676, 1004, 1031
Bso31I GGTCTC 1 cut(s) 840
BsoBI CYCGRG 2 cut(s) 98, 533
Bsp1286I GDGCHC 3 cut(s) 550, 657, 862
Bsp1407I TGTACA 1 cut(s) 157
Bsp143I GATC 3 cut(s) 220, 961, 985
Bsp19I CCATGG 1 cut(s) 990
BspACI CCGC 1 cut(s) 881
BspANI GGCC 4 cut(s) 497, 676, 1004, 1031
BspCNI CTCAG 5 cut(s) 182, 460, 595, 669, 750
BspFNI CGCG 1 cut(s) 881
BspHI TCATGA 1 cut(s) 955
BspOI GCTAGC 1 cut(s) 54
BspPI GGATC 2 cut(s) 956, 980
BspTI CTTAAG 1 cut(s) 971
BspTNI GGTCTC 1 cut(s) 840
BsrGI TGTACA 1 cut(s) 157
BsrI ACTGG 1 cut(s) 691
BssECI CCNNGG 2 cut(s) 27, 990
BssMI GATC 3 cut(s) 220, 961, 985
BssT1I CCWWGG 1 cut(s) 990
Bst2UI CCWGG 3 cut(s) 28, 851, 1006
Bst4CI ACNGT 2 cut(s) 292, 611
Bst6I CTCTTC 3 cut(s) 833, 838, 972
BstAFI CTTAAG 1 cut(s) 971
BstAUI TGTACA 1 cut(s) 157
BstC8I GCNNGC 3 cut(s) 52, 329, 714
BstDEI CTNAG 8 cut(s) 107, 169, 228, 275, 468, 603, 656, 758
BstDSI CCRYGG 1 cut(s) 990
BstF5I GGATG 1 cut(s) 1000
BstFNI CGCG 1 cut(s) 881
BstKTI GATC 3 cut(s) 223, 964, 988
BstMAI GTCTC 2 cut(s) 582, 840
BstMBI GATC 3 cut(s) 220, 961, 985
BstMWI GCNNNNNNNGC 3 cut(s) 51, 722, 830
BstNI CCWGG 3 cut(s) 28, 851, 1006
BstNSI RCATGY 1 cut(s) 812
BstSCI CCNGG 3 cut(s) 26, 849, 1004
BstSFI CTRYAG 1 cut(s) 150
BstSLI GKGCMC 1 cut(s) 862
BstUI CGCG 1 cut(s) 881
BstV1I GCAGC 2 cut(s) 498, 703
BsuI GTATCC 1 cut(s) 175
BsuRI GGCC 4 cut(s) 497, 676, 1004, 1031
BtgI CCRYGG 1 cut(s) 990
BtgZI GCGATG 1 cut(s) 907
BtsCI GGATG 1 cut(s) 1000
BtsI GCAGTG 1 cut(s) 406
BtsIMutI CAGTG 2 cut(s) 288, 406
Cac8I GCNNGC 3 cut(s) 52, 329, 714
CaiI CAGNNNCTG 2 cut(s) 757, 892
CciI TCATGA 1 cut(s) 955
Cfr13I GGNCC 1 cut(s) 495
Csp6I GTAC 3 cut(s) 158, 272, 785
CspCI CAANNNNNGTGG 2 cut(s) 273, 308
CviAII CATG 7 cut(s) 238, 581, 590, 629, 809, 956, 991
CviQI GTAC 3 cut(s) 158, 272, 785
DdeI CTNAG 8 cut(s) 107, 169, 228, 275, 468, 603, 656, 758
DpnI GATC 3 cut(s) 222, 963, 987
DpnII GATC 3 cut(s) 220, 961, 985
EaeI YGGCCR 1 cut(s) 1002
Eam1104I CTCTTC 3 cut(s) 833, 838, 972
EarI CTCTTC 3 cut(s) 833, 838, 972
Ecl136II GAGCTC 2 cut(s) 548, 655
Eco130I CCWWGG 1 cut(s) 990
Eco24I GRGCYC 2 cut(s) 550, 657
Eco31I GGTCTC 1 cut(s) 840
Eco53kI GAGCTC 2 cut(s) 548, 655
Eco88I CYCGRG 2 cut(s) 98, 533
EcoICRI GAGCTC 2 cut(s) 548, 655
EcoRII CCWGG 3 cut(s) 26, 849, 1004
EcoT14I CCWWGG 1 cut(s) 990
EcoT22I ATGCAT 1 cut(s) 557
EcoT38I GRGCYC 2 cut(s) 550, 657
ErhI CCWWGG 1 cut(s) 990
FaeI CATG 7 cut(s) 241, 584, 593, 632, 812, 959, 994
FaqI GGGAC 1 cut(s) 245
FatI CATG 7 cut(s) 237, 580, 589, 628, 808, 955, 990
FbaI TGATCA 1 cut(s) 220
Fnu4HI GCNGC 2 cut(s) 487, 717
FokI GGATG 1 cut(s) 1007
FriOI GRGCYC 2 cut(s) 550, 657
Fsp4HI GCNGC 2 cut(s) 487, 717
FspBI CTAG 4 cut(s) 5, 51, 324, 648
GluI GCNGC 2 cut(s) 487, 717
GsuI CTGGAG 1 cut(s) 564
HaeIII GGCC 4 cut(s) 497, 676, 1004, 1031
Hin1II CATG 7 cut(s) 241, 584, 593, 632, 812, 959, 994
HincII GTYRAC 1 cut(s) 1011
HindII GTYRAC 1 cut(s) 1011
HindIII AAGCTT 1 cut(s) 344
HinfI GANTC 5 cut(s) 358, 430, 817, 876, 889
HphI GGTGA 2 cut(s) 647, 910
Hpy166II GTNNAC 2 cut(s) 910, 1011
Hpy188I TCNGA 7 cut(s) 220, 246, 363, 429, 604, 659, 931
Hpy188III TCNNGA 7 cut(s) 35, 454, 533, 535, 543, 767, 956
Hpy8I GTNNAC 2 cut(s) 910, 1011
Hpy99I CGWCG 1 cut(s) 802
HpyAV CCTTC 4 cut(s) 76, 199, 635, 848
HpyCH4III ACNGT 2 cut(s) 292, 611
HpyCH4V TGCA 7 cut(s) 45, 399, 555, 725, 824, 886, 940
HpyF10VI GCNNNNNNNGC 3 cut(s) 51, 722, 830
HpyF3I CTNAG 8 cut(s) 107, 169, 228, 275, 468, 603, 656, 758
Hsp92II CATG 7 cut(s) 241, 584, 593, 632, 812, 959, 994
Ksp22I TGATCA 1 cut(s) 220
Kzo9I GATC 3 cut(s) 220, 961, 985
LmnI GCTCC 2 cut(s) 29, 545
Lsp1109I GCAGC 2 cut(s) 498, 703
LweI GCATC 5 cut(s) 306, 542, 552, 712, 985
MaeI CTAG 4 cut(s) 5, 51, 324, 648
MaeIII GTNAC 1 cut(s) 814
MalI GATC 3 cut(s) 222, 963, 987
MboI GATC 3 cut(s) 220, 961, 985
MboII GAAGA 5 cut(s) 86, 679, 850, 855, 959
MfeI CAATTG 1 cut(s) 490
MhlI GDGCHC 3 cut(s) 550, 657, 862
MlsI TGGCCA 1 cut(s) 1004
MluCI AATT 4 cut(s) 265, 490, 702, 935
MluNI TGGCCA 1 cut(s) 1004
MlyI GAGTC 2 cut(s) 367, 811
Mox20I TGGCCA 1 cut(s) 1004
Mph1103I ATGCAT 1 cut(s) 557
MscI TGGCCA 1 cut(s) 1004
MseI TTAA 3 cut(s) 731, 872, 972
MslI CAYNNNNRTG 2 cut(s) 560, 633
Msp20I TGGCCA 1 cut(s) 1004
MspCI CTTAAG 1 cut(s) 971
MspR9I CCNGG 3 cut(s) 28, 851, 1006
MunI CAATTG 1 cut(s) 490
Mva1269I GAATGC 1 cut(s) 383
MvaI CCWGG 3 cut(s) 28, 851, 1006
MvnI CGCG 1 cut(s) 881
MwoI GCNNNNNNNGC 3 cut(s) 51, 722, 830
NcoI CCATGG 1 cut(s) 990
NdeII GATC 3 cut(s) 220, 961, 985
NheI GCTAGC 1 cut(s) 50
NlaIII CATG 7 cut(s) 241, 584, 593, 632, 812, 959, 994
NmuCI GTSAC 1 cut(s) 814
NsiI ATGCAT 1 cut(s) 557
NspI RCATGY 1 cut(s) 812
PaeR7I CTCGAG 1 cut(s) 533
PagI TCATGA 1 cut(s) 955
PciI ACATGT 1 cut(s) 808
PctI GAATGC 1 cut(s) 383
PfeI GAWTC 3 cut(s) 430, 876, 889
PflFI GACNNNGTC 1 cut(s) 61
PflMI CCANNNNNTGG 1 cut(s) 1020
PkrI GCNGC 2 cut(s) 488, 718
PleI GAGTC 2 cut(s) 366, 811
PpsI GAGTC 2 cut(s) 366, 811
PscI ACATGT 1 cut(s) 808
Psp124BI GAGCTC 2 cut(s) 550, 657
Psp6I CCWGG 3 cut(s) 26, 849, 1004
PspGI CCWGG 3 cut(s) 26, 849, 1004
PspPI GGNCC 1 cut(s) 495
PstNI CAGNNNCTG 2 cut(s) 757, 892
PsyI GACNNNGTC 1 cut(s) 61
RsaI GTAC 3 cut(s) 159, 273, 786
RsaNI GTAC 3 cut(s) 158, 272, 785
RseI CAYNNNNRTG 2 cut(s) 560, 633
SacI GAGCTC 2 cut(s) 550, 657
SaqAI TTAA 3 cut(s) 731, 872, 972
SatI GCNGC 2 cut(s) 487, 717
Sau3AI GATC 3 cut(s) 220, 961, 985
Sau96I GGNCC 1 cut(s) 495
SchI GAGTC 2 cut(s) 367, 811
ScrFI CCNGG 3 cut(s) 28, 851, 1006
SduI GDGCHC 3 cut(s) 550, 657, 862
SfaNI GCATC 5 cut(s) 306, 542, 552, 712, 985
SfcI CTRYAG 1 cut(s) 150
Sfr274I CTCGAG 1 cut(s) 533
SlaI CTCGAG 1 cut(s) 533
SmiMI CAYNNNNRTG 2 cut(s) 560, 633
SmlI CTYRAG 3 cut(s) 452, 533, 971
SmoI CTYRAG 3 cut(s) 452, 533, 971
Sse9I AATT 4 cut(s) 265, 490, 702, 935
SsiI CCGC 1 cut(s) 881
SspI AATATT 1 cut(s) 479
SspMI CTAG 4 cut(s) 5, 51, 324, 648
SstI GAGCTC 2 cut(s) 550, 657
StyD4I CCNGG 3 cut(s) 26, 849, 1004
StyI CCWWGG 1 cut(s) 990
TaaI ACNGT 2 cut(s) 292, 611
TaqI TCGA 4 cut(s) 534, 550, 681, 828
TasI AATT 4 cut(s) 265, 490, 702, 935
TatI WGTACW 2 cut(s) 157, 271
TfiI GAWTC 3 cut(s) 430, 876, 889
Tru1I TTAA 3 cut(s) 731, 872, 972
Tru9I TTAA 3 cut(s) 731, 872, 972
TscAI CASTG 2 cut(s) 295, 406
TseFI GTSAC 1 cut(s) 814
TseI GCWGC 2 cut(s) 486, 716
Tsp45I GTSAC 1 cut(s) 814
TspDTI ATGAA 4 cut(s) 488, 606, 617, 948
TspRI CASTG 2 cut(s) 295, 406
Tth111I GACNNNGTC 1 cut(s) 61
Van91I CCANNNNNTGG 1 cut(s) 1020
Vha464I CTTAAG 1 cut(s) 971
XceI RCATGY 1 cut(s) 812
XcmI CCANNNNNNNNNTGG 1 cut(s) 997
XhoI CTCGAG 1 cut(s) 533
XspI CTAG 4 cut(s) 5, 51, 324, 648
Zsp2I ATGCAT 1 cut(s) 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.