Rroxscaffold_2G00152230

Asp/Glu/Hydantoin racemase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002475
Physical Location & Seq
Reverse (-)
89273672 .. 89275276
1605 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00152230.1

Sequence Viewer

Length: 990 bp
ATGTCCTTCCACACCCAAAATCGTCCTACTCTTCTTCTCGGTGTTCTAAGCGAAAACCGAACCCAAAACAGAACCAAATCAAGCCCATCTGTAGCTGTACAAGTATCCTCAGTCCTACAAACTAATGGAAAAAGTGGAAATGGGAATGGAAATCTATCTGATCAGTCTAAGAAAGGCATGGCTCTGAAAAAGTGTCAAGTCCCCAATTTTGTACTAAGCCAACCAAACACTGTGGGCATCATTGGAGGAGTGTCTGTATATTCTAGCCTGCTTTTCTTGGAGAAGCTTGTTTGGTGGAGTCTGAAAGATGGAGGTGAATGCCCACCTTTTCTTGTGTGCAGTGATACAACACAATATAAAGAGCTTCCGATTCGCAGTTTGTTTCAATCACTCAAGAGAAAAGCTACTGAGATGAAATATTCAAGCAGCAATTGGGCCATCATTGACAGCTTGTGTCAAAAAAGGGCATTTCTCGAGAACTCTGGAGCTCGATGCATAGTCATGCCATGCCATCTGTCTCATGCTTGGCATGAACAGATTTCTCAGGACTGTTCTTTGAGTTTCCTTCATGTTGGTGAGTGTGTTGCTAGAGAGCTCAGAGAAGCGAAGATGAGGCCACTCGAAACTGGTAGTAATGTGAGAATTGGGGTGCTTGCTGCTCATGCAACTTTAACAGCTGGTTTTTACCAGCAGAAGCTAAGAAATCAGGACTTTGAGGTGGTTGTACCAGACAAAGCGACGATGGAACATGTTGTGACTCCAGCAATCGAGGCATTGAAGAGAAGAGACCTGGAAGGGGCACGAAATCTGTTAAGAATCGCGGTGCAGATTCTGTTGGTGAGGGGTGTGAACATCGTCATCATCGCTTCTGATGAATTGCAGGGTGTTCTGCCTCATGATGATCCTCTTCTTAAGAAATGTATTGATCCCATGGATGCTTTGGCCAGGTCAACCATAAAATGGGCAAAGGCCACCGGAAATGGTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asp_Glu_race

Protein Analysis

329

Amino Acids

36.21

Weight (kDa)

9.2

Isoelectric Point (pI)

38.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp_Glu_race PF01177 141 - 317 2.6e-23 Asp/Glu/Hydantoin racemase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 960
AccII CGCG 1 cut(s) 821
AciI CCGC 1 cut(s) 821
AclWI GGATC 2 cut(s) 896, 920
AcoI YGGCCR 1 cut(s) 942
AfaI GTAC 3 cut(s) 99, 213, 726
AfiI CCNNNNNNNGG 2 cut(s) 796, 960
AflII CTTAAG 1 cut(s) 911
AflIII ACRYGT 1 cut(s) 748
AgsI TTSAA 3 cut(s) 386, 423, 778
AjnI CCWGG 2 cut(s) 789, 944
AluBI AGCT 9 cut(s) 95, 286, 364, 404, 450, 488, 595, 677, 697
AluI AGCT 9 cut(s) 95, 286, 364, 404, 450, 488, 595, 677, 697
Alw21I GWGCWC 2 cut(s) 490, 597
Alw26I GTCTC 2 cut(s) 522, 780
AlwI GGATC 2 cut(s) 896, 920
AlwNI CAGNNNCTG 1 cut(s) 832
Ama87I CYCGRG 1 cut(s) 473
AoxI GGCC 4 cut(s) 435, 614, 942, 969
ApeKI GCWGC 2 cut(s) 426, 656
AspS9I GGNCC 1 cut(s) 435
AsuHPI GGTGA 3 cut(s) 326, 587, 850
AvaI CYCGRG 1 cut(s) 473
BaeGI GKGCMC 1 cut(s) 802
BalI TGGCCA 1 cut(s) 944
BanII GRGCYC 2 cut(s) 490, 597
Bbv12I GWGCWC 2 cut(s) 490, 597
BbvI GCAGC 2 cut(s) 438, 643
BccI CCATC 5 cut(s) 94, 302, 446, 519, 736
BciT130I CCWGG 2 cut(s) 791, 946
BciVI GTATCC 1 cut(s) 115
BclI TGATCA 1 cut(s) 160
BcoDI GTCTC 2 cut(s) 522, 780
BfaI CTAG 2 cut(s) 264, 588
BfmI CTRYAG 1 cut(s) 90
BfrI CTTAAG 1 cut(s) 911
BfuI GTATCC 1 cut(s) 115
BisI GCNGC 2 cut(s) 427, 657
BlsI GCNGC 2 cut(s) 428, 658
Bme1390I CCNGG 2 cut(s) 791, 946
BmeT110I CYCGRG 1 cut(s) 473
BmgT120I GGNCC 1 cut(s) 435
BmrFI CCNGG 2 cut(s) 791, 946
BmsI GCATC 3 cut(s) 246, 482, 925
BpmI CTGGAG 2 cut(s) 504, 744
BpuEI CTTGAG 1 cut(s) 377
BsaI GGTCTC 1 cut(s) 780
BsaJI CCNNGG 1 cut(s) 930
BsaWI WCCGGW 1 cut(s) 974
BsaXI ACNNNNNCTCC 2 cut(s) 240, 270
Bsc4I CCNNNNNNNGG 2 cut(s) 796, 960
Bse1I ACTGG 1 cut(s) 631
BseBI CCWGG 2 cut(s) 791, 946
BseDI CCNNGG 1 cut(s) 930
BseGI GGATG 1 cut(s) 940
BseLI CCNNNNNNNGG 2 cut(s) 796, 960
BseMII CTCAG 4 cut(s) 123, 399, 557, 610
BseNI ACTGG 1 cut(s) 631
BseRI GAGGAG 1 cut(s) 261
BseSI GKGCMC 1 cut(s) 802
BseXI GCAGC 2 cut(s) 438, 643
BsgI GTGCAG 2 cut(s) 358, 845
Bsh1236I CGCG 1 cut(s) 821
BshFI GGCC 4 cut(s) 437, 616, 944, 971
BsiHKAI GWGCWC 2 cut(s) 490, 597
BsiHKCI CYCGRG 1 cut(s) 473
BsiSI CCGG 1 cut(s) 975
BslFI GGGAC 1 cut(s) 185
BslI CCNNNNNNNGG 2 cut(s) 796, 960
BsmAI GTCTC 2 cut(s) 522, 780
BsmFI GGGAC 1 cut(s) 185
BsmI GAATGC 1 cut(s) 323
BsnI GGCC 4 cut(s) 437, 616, 944, 971
Bso31I GGTCTC 1 cut(s) 780
BsoBI CYCGRG 1 cut(s) 473
Bsp1286I GDGCHC 3 cut(s) 490, 597, 802
Bsp1407I TGTACA 1 cut(s) 97
Bsp143I GATC 3 cut(s) 160, 901, 925
Bsp19I CCATGG 1 cut(s) 930
BspACI CCGC 1 cut(s) 821
BspANI GGCC 4 cut(s) 437, 616, 944, 971
BspCNI CTCAG 4 cut(s) 122, 400, 556, 609
BspFNI CGCG 1 cut(s) 821
BspHI TCATGA 1 cut(s) 895
BspPI GGATC 2 cut(s) 896, 920
BspTI CTTAAG 1 cut(s) 911
BspTNI GGTCTC 1 cut(s) 780
BsrGI TGTACA 1 cut(s) 97
BsrI ACTGG 1 cut(s) 631
BssECI CCNNGG 1 cut(s) 930
BssMI GATC 3 cut(s) 160, 901, 925
BssT1I CCWWGG 1 cut(s) 930
Bst2UI CCWGG 2 cut(s) 791, 946
Bst4CI ACNGT 2 cut(s) 232, 551
Bst6I CTCTTC 4 cut(s) 36, 773, 778, 912
BstAFI CTTAAG 1 cut(s) 911
BstAUI TGTACA 1 cut(s) 97
BstC8I GCNNGC 2 cut(s) 269, 654
BstDEI CTNAG 8 cut(s) 47, 109, 168, 215, 408, 543, 596, 698
BstDSI CCRYGG 1 cut(s) 930
BstF5I GGATG 1 cut(s) 940
BstFNI CGCG 1 cut(s) 821
BstKTI GATC 3 cut(s) 163, 904, 928
BstMAI GTCTC 2 cut(s) 522, 780
BstMBI GATC 3 cut(s) 160, 901, 925
BstMWI GCNNNNNNNGC 2 cut(s) 662, 770
BstNI CCWGG 2 cut(s) 791, 946
BstNSI RCATGY 1 cut(s) 752
BstSCI CCNGG 2 cut(s) 789, 944
BstSFI CTRYAG 1 cut(s) 90
BstSLI GKGCMC 1 cut(s) 802
BstUI CGCG 1 cut(s) 821
BstV1I GCAGC 2 cut(s) 438, 643
BsuI GTATCC 1 cut(s) 115
BsuRI GGCC 4 cut(s) 437, 616, 944, 971
BtgI CCRYGG 1 cut(s) 930
BtgZI GCGATG 1 cut(s) 847
BtsCI GGATG 1 cut(s) 940
BtsI GCAGTG 1 cut(s) 346
BtsIMutI CAGTG 2 cut(s) 228, 346
Cac8I GCNNGC 2 cut(s) 269, 654
CaiI CAGNNNCTG 1 cut(s) 832
CciI TCATGA 1 cut(s) 895
Cfr13I GGNCC 1 cut(s) 435
Csp6I GTAC 3 cut(s) 98, 212, 725
CspCI CAANNNNNGTGG 2 cut(s) 213, 248
CviQI GTAC 3 cut(s) 98, 212, 725
DdeI CTNAG 8 cut(s) 47, 109, 168, 215, 408, 543, 596, 698
DpnI GATC 3 cut(s) 162, 903, 927
DpnII GATC 3 cut(s) 160, 901, 925
EaeI YGGCCR 1 cut(s) 942
Eam1104I CTCTTC 4 cut(s) 36, 773, 778, 912
EarI CTCTTC 4 cut(s) 36, 773, 778, 912
Ecl136II GAGCTC 2 cut(s) 488, 595
Eco130I CCWWGG 1 cut(s) 930
Eco24I GRGCYC 2 cut(s) 490, 597
Eco31I GGTCTC 1 cut(s) 780
Eco53kI GAGCTC 2 cut(s) 488, 595
Eco88I CYCGRG 1 cut(s) 473
EcoICRI GAGCTC 2 cut(s) 488, 595
EcoRII CCWGG 2 cut(s) 789, 944
EcoT14I CCWWGG 1 cut(s) 930
EcoT22I ATGCAT 1 cut(s) 497
EcoT38I GRGCYC 2 cut(s) 490, 597
ErhI CCWWGG 1 cut(s) 930
FaqI GGGAC 1 cut(s) 185
FbaI TGATCA 1 cut(s) 160
Fnu4HI GCNGC 2 cut(s) 427, 657
FokI GGATG 1 cut(s) 947
FriOI GRGCYC 2 cut(s) 490, 597
Fsp4HI GCNGC 2 cut(s) 427, 657
FspBI CTAG 2 cut(s) 264, 588
GluI GCNGC 2 cut(s) 427, 657
GsuI CTGGAG 2 cut(s) 504, 744
HaeIII GGCC 4 cut(s) 437, 616, 944, 971
HapII CCGG 1 cut(s) 975
HincII GTYRAC 1 cut(s) 951
HindII GTYRAC 1 cut(s) 951
HindIII AAGCTT 1 cut(s) 284
HinfI GANTC 5 cut(s) 298, 370, 757, 816, 829
HpaII CCGG 1 cut(s) 975
HphI GGTGA 3 cut(s) 326, 587, 850
Hpy166II GTNNAC 2 cut(s) 850, 951
Hpy188I TCNGA 6 cut(s) 160, 186, 303, 369, 599, 871
Hpy188III TCNNGA 7 cut(s) 394, 473, 475, 483, 545, 707, 896
Hpy8I GTNNAC 2 cut(s) 850, 951
Hpy99I CGWCG 1 cut(s) 742
HpyAV CCTTC 3 cut(s) 16, 575, 788
HpyCH4III ACNGT 2 cut(s) 232, 551
HpyCH4V TGCA 5 cut(s) 339, 495, 665, 826, 880
HpyF10VI GCNNNNNNNGC 2 cut(s) 662, 770
HpyF3I CTNAG 8 cut(s) 47, 109, 168, 215, 408, 543, 596, 698
Ksp22I TGATCA 1 cut(s) 160
Kzo9I GATC 3 cut(s) 160, 901, 925
LmnI GCTCC 1 cut(s) 485
Lsp1109I GCAGC 2 cut(s) 438, 643
LweI GCATC 3 cut(s) 246, 482, 925
MaeI CTAG 2 cut(s) 264, 588
MaeIII GTNAC 1 cut(s) 754
MalI GATC 3 cut(s) 162, 903, 927
MboI GATC 3 cut(s) 160, 901, 925
MboII GAAGA 6 cut(s) 23, 26, 619, 790, 795, 899
MfeI CAATTG 1 cut(s) 430
MhlI GDGCHC 3 cut(s) 490, 597, 802
MlsI TGGCCA 1 cut(s) 944
MluCI AATT 4 cut(s) 205, 430, 642, 875
MluNI TGGCCA 1 cut(s) 944
MlyI GAGTC 2 cut(s) 307, 751
MnlI CCTC 9 cut(s) 118, 239, 305, 606, 709, 763, 834, 903, 915
Mox20I TGGCCA 1 cut(s) 944
Mph1103I ATGCAT 1 cut(s) 497
MscI TGGCCA 1 cut(s) 944
MseI TTAA 3 cut(s) 671, 812, 912
MslI CAYNNNNRTG 2 cut(s) 500, 573
Msp20I TGGCCA 1 cut(s) 944
MspA1I CMGCKG 1 cut(s) 677
MspCI CTTAAG 1 cut(s) 911
MspI CCGG 1 cut(s) 975
MspR9I CCNGG 2 cut(s) 791, 946
MunI CAATTG 1 cut(s) 430
Mva1269I GAATGC 1 cut(s) 323
MvaI CCWGG 2 cut(s) 791, 946
MvnI CGCG 1 cut(s) 821
MwoI GCNNNNNNNGC 2 cut(s) 662, 770
NcoI CCATGG 1 cut(s) 930
NdeII GATC 3 cut(s) 160, 901, 925
NmuCI GTSAC 1 cut(s) 754
NsiI ATGCAT 1 cut(s) 497
NspI RCATGY 1 cut(s) 752
PaeR7I CTCGAG 1 cut(s) 473
PagI TCATGA 1 cut(s) 895
PciI ACATGT 1 cut(s) 748
PctI GAATGC 1 cut(s) 323
PfeI GAWTC 3 cut(s) 370, 816, 829
PflMI CCANNNNNTGG 1 cut(s) 960
PkrI GCNGC 2 cut(s) 428, 658
PleI GAGTC 2 cut(s) 306, 751
PpsI GAGTC 2 cut(s) 306, 751
PscI ACATGT 1 cut(s) 748
Psp124BI GAGCTC 2 cut(s) 490, 597
Psp6I CCWGG 2 cut(s) 789, 944
PspGI CCWGG 2 cut(s) 789, 944
PspPI GGNCC 1 cut(s) 435
PstNI CAGNNNCTG 1 cut(s) 832
PvuII CAGCTG 1 cut(s) 677
RsaI GTAC 3 cut(s) 99, 213, 726
RsaNI GTAC 3 cut(s) 98, 212, 725
RseI CAYNNNNRTG 2 cut(s) 500, 573
SacI GAGCTC 2 cut(s) 490, 597
SaqAI TTAA 3 cut(s) 671, 812, 912
SatI GCNGC 2 cut(s) 427, 657
Sau3AI GATC 3 cut(s) 160, 901, 925
Sau96I GGNCC 1 cut(s) 435
SchI GAGTC 2 cut(s) 307, 751
ScrFI CCNGG 2 cut(s) 791, 946
SduI GDGCHC 3 cut(s) 490, 597, 802
SfaNI GCATC 3 cut(s) 246, 482, 925
SfcI CTRYAG 1 cut(s) 90
Sfr274I CTCGAG 1 cut(s) 473
SlaI CTCGAG 1 cut(s) 473
SmiMI CAYNNNNRTG 2 cut(s) 500, 573
SmlI CTYRAG 3 cut(s) 392, 473, 911
SmoI CTYRAG 3 cut(s) 392, 473, 911
Sse9I AATT 4 cut(s) 205, 430, 642, 875
SsiI CCGC 1 cut(s) 821
SspI AATATT 1 cut(s) 419
SspMI CTAG 2 cut(s) 264, 588
SstI GAGCTC 2 cut(s) 490, 597
StyD4I CCNGG 2 cut(s) 789, 944
StyI CCWWGG 1 cut(s) 930
TaaI ACNGT 2 cut(s) 232, 551
TaqI TCGA 4 cut(s) 474, 490, 621, 768
TasI AATT 4 cut(s) 205, 430, 642, 875
TatI WGTACW 2 cut(s) 97, 211
TfiI GAWTC 3 cut(s) 370, 816, 829
Tru1I TTAA 3 cut(s) 671, 812, 912
Tru9I TTAA 3 cut(s) 671, 812, 912
TscAI CASTG 2 cut(s) 235, 346
TseFI GTSAC 1 cut(s) 754
TseI GCWGC 2 cut(s) 426, 656
Tsp45I GTSAC 1 cut(s) 754
TspDTI ATGAA 4 cut(s) 428, 546, 557, 888
TspRI CASTG 2 cut(s) 235, 346
Van91I CCANNNNNTGG 1 cut(s) 960
Vha464I CTTAAG 1 cut(s) 911
XceI RCATGY 1 cut(s) 752
XcmI CCANNNNNNNNNTGG 1 cut(s) 937
XhoI CTCGAG 1 cut(s) 473
XspI CTAG 2 cut(s) 264, 588
Zsp2I ATGCAT 1 cut(s) 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.