Rorug02G0331600

Asp/Glu/Hydantoin racemase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002475
Physical Location & Seq
Forward (+)
41137163 .. 41137543
381 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0331600.1

Sequence Viewer

Length: 381 bp
ATGCTCGTGGAGTCGGAGATCCTCCGCTTCAAGGCCTACAAGCCCATCCTTCTGCTCGGACGGCACCGTTTCGTCGGTGTCGACATGAGGATCCGCGTTAAGGGAGGAGGTCACACTTCTCAGATCTACGCCATCCATCAGAGCATCGCCAAGGCCTTGGTTGCTTATTACCAGAAGTTCGTGGATGAGCAGAGCAAGAAGGAAATCAAGGACATTCTCGTCATGTATGACCGTACTCTCCTCGTCGCTGATCCAAGGCGTTGCGAGCCAGAGCTAGGTTTCGAGTGGCTGCTGTTGTCGGTTTCGAATTGCCCCTTTCCGAGACGGACCGATTCGGACCAGATTTGTATGAGGCATAGGGATTGTGGTCGTGATCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asp_Glu_race

Protein Analysis

126

Amino Acids

14.73

Weight (kDa)

9.16

Isoelectric Point (pI)

54.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S9 PF00380 4 - 90 1e-16 Ribosomal protein S9/S16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 218
AccB1I GGYRCC 1 cut(s) 63
AccI GTMKAC 1 cut(s) 81
AccII CGCG 1 cut(s) 96
AciI CCGC 2 cut(s) 25, 94
AclWI GGATC 4 cut(s) 13, 85, 98, 245
AfaI GTAC 1 cut(s) 235
AfiI CCNNNNNNNGG 3 cut(s) 31, 100, 275
AgsI TTSAA 1 cut(s) 31
AluBI AGCT 1 cut(s) 274
AluI AGCT 1 cut(s) 274
Alw26I GTCTC 1 cut(s) 316
AlwI GGATC 4 cut(s) 13, 85, 98, 245
AoxI GGCC 2 cut(s) 33, 153
ApeKI GCWGC 1 cut(s) 289
AspS9I GGNCC 2 cut(s) 327, 337
AsuII TTCGAA 1 cut(s) 305
AvaII GGWCC 2 cut(s) 327, 337
BamHI GGATCC 1 cut(s) 90
BanI GGYRCC 1 cut(s) 63
BauI CACGAG 1 cut(s) 5
BbvI GCAGC 1 cut(s) 276
BccI CCATC 3 cut(s) 53, 140, 144
BceAI ACGGC 1 cut(s) 77
BclI TGATCA 1 cut(s) 373
BcoDI GTCTC 1 cut(s) 316
BfaI CTAG 1 cut(s) 275
BglII AGATCT 1 cut(s) 123
BisI GCNGC 1 cut(s) 290
BlsI GCNGC 1 cut(s) 291
Bme18I GGWCC 2 cut(s) 327, 337
BmgT120I GGNCC 2 cut(s) 327, 337
BmiI GGNNCC 2 cut(s) 65, 92
BmsI GCATC 1 cut(s) 153
Bpu14I TTCGAA 1 cut(s) 305
BsaJI CCNNGG 3 cut(s) 150, 156, 254
Bsc4I CCNNNNNNNGG 3 cut(s) 31, 100, 275
BseDI CCNNGG 3 cut(s) 150, 156, 254
BseGI GGATG 3 cut(s) 45, 132, 190
BseLI CCNNNNNNNGG 3 cut(s) 31, 100, 275
BseMII CTCAG 1 cut(s) 134
BseRI GAGGAG 2 cut(s) 120, 230
BseXI GCAGC 1 cut(s) 276
Bsh1236I CGCG 1 cut(s) 96
BshFI GGCC 2 cut(s) 35, 155
BshNI GGYRCC 1 cut(s) 63
BslI CCNNNNNNNGG 3 cut(s) 31, 100, 275
BsmAI GTCTC 1 cut(s) 316
BsmBI CGTCTC 1 cut(s) 316
BsnI GGCC 2 cut(s) 35, 155
Bsp119I TTCGAA 1 cut(s) 305
Bsp143I GATC 5 cut(s) 18, 90, 123, 250, 373
BspACI CCGC 2 cut(s) 25, 94
BspANI GGCC 2 cut(s) 35, 155
BspCNI CTCAG 1 cut(s) 133
BspFNI CGCG 1 cut(s) 96
BspLI GGNNCC 2 cut(s) 65, 92
BspPI GGATC 4 cut(s) 13, 85, 98, 245
BspT104I TTCGAA 1 cut(s) 305
BspT107I GGYRCC 1 cut(s) 63
BssECI CCNNGG 3 cut(s) 150, 156, 254
BssMI GATC 5 cut(s) 18, 90, 123, 250, 373
BssSI CACGAG 1 cut(s) 5
BssT1I CCWWGG 3 cut(s) 150, 156, 254
Bst2BI CACGAG 1 cut(s) 5
Bst4CI ACNGT 2 cut(s) 68, 233
BstBI TTCGAA 1 cut(s) 305
BstC8I GCNNGC 1 cut(s) 266
BstDEI CTNAG 1 cut(s) 120
BstF5I GGATG 3 cut(s) 45, 132, 190
BstFNI CGCG 1 cut(s) 96
BstKTI GATC 5 cut(s) 21, 93, 126, 253, 376
BstMAI GTCTC 1 cut(s) 316
BstMBI GATC 5 cut(s) 18, 90, 123, 250, 373
BstMWI GCNNNNNNNGC 3 cut(s) 61, 161, 265
BstUI CGCG 1 cut(s) 96
BstV1I GCAGC 1 cut(s) 276
BstX2I RGATCY 3 cut(s) 18, 90, 123
BstXI CCANNNNNNTGG 1 cut(s) 157
BstYI RGATCY 3 cut(s) 18, 90, 123
BsuRI GGCC 2 cut(s) 35, 155
BtgZI GCGATG 1 cut(s) 130
BtsCI GGATG 3 cut(s) 45, 132, 190
Cac8I GCNNGC 1 cut(s) 266
Cfr13I GGNCC 2 cut(s) 327, 337
CpoI CGGWCCG 1 cut(s) 327
Csp6I GTAC 1 cut(s) 234
CspI CGGWCCG 1 cut(s) 327
CviAII CATG 2 cut(s) 85, 223
CviJI RGCY 6 cut(s) 35, 43, 155, 268, 274, 289
CviKI_1 RGCY 6 cut(s) 35, 43, 155, 268, 274, 289
CviQI GTAC 1 cut(s) 234
DdeI CTNAG 1 cut(s) 120
DpnI GATC 5 cut(s) 20, 92, 125, 252, 375
DpnII GATC 5 cut(s) 18, 90, 123, 250, 373
DrdI GACNNNNNNGTC 1 cut(s) 218
DseDI GACNNNNNNGTC 1 cut(s) 218
Eco130I CCWWGG 3 cut(s) 150, 156, 254
Eco147I AGGCCT 2 cut(s) 35, 155
Eco47I GGWCC 2 cut(s) 327, 337
EcoT14I CCWWGG 3 cut(s) 150, 156, 254
ErhI CCWWGG 3 cut(s) 150, 156, 254
Esp3I CGTCTC 1 cut(s) 316
FaeI CATG 2 cut(s) 88, 226
FaiI YATR 5 cut(s) 86, 224, 228, 350, 357
FatI CATG 2 cut(s) 84, 222
FbaI TGATCA 1 cut(s) 373
FblI GTMKAC 1 cut(s) 81
Fnu4HI GCNGC 1 cut(s) 290
FokI GGATG 3 cut(s) 32, 119, 197
Fsp4HI GCNGC 1 cut(s) 290
FspBI CTAG 1 cut(s) 275
GluI GCNGC 1 cut(s) 290
HaeIII GGCC 2 cut(s) 35, 155
Hin1II CATG 2 cut(s) 88, 226
HincII GTYRAC 1 cut(s) 82
HindII GTYRAC 1 cut(s) 82
HinfI GANTC 2 cut(s) 11, 332
Hpy166II GTNNAC 1 cut(s) 82
Hpy188I TCNGA 6 cut(s) 16, 59, 123, 141, 321, 337
Hpy188III TCNNGA 1 cut(s) 371
Hpy8I GTNNAC 1 cut(s) 82
Hpy99I CGWCG 2 cut(s) 77, 248
HpyAV CCTTC 2 cut(s) 59, 193
HpyCH4III ACNGT 2 cut(s) 68, 233
HpyF10VI GCNNNNNNNGC 3 cut(s) 61, 161, 265
HpyF3I CTNAG 1 cut(s) 120
Hsp92II CATG 2 cut(s) 88, 226
Ksp22I TGATCA 1 cut(s) 373
Kzo9I GATC 5 cut(s) 18, 90, 123, 250, 373
LpnPI CCDG 3 cut(s) 185, 282, 353
Lsp1109I GCAGC 1 cut(s) 276
LweI GCATC 1 cut(s) 153
MaeI CTAG 1 cut(s) 275
MaeIII GTNAC 1 cut(s) 110
MalI GATC 5 cut(s) 20, 92, 125, 252, 375
MboI GATC 5 cut(s) 18, 90, 123, 250, 373
MflI RGATCY 3 cut(s) 18, 90, 123
MluCI AATT 1 cut(s) 307
MlyI GAGTC 1 cut(s) 20
MnlI CCTC 6 cut(s) 32, 81, 98, 101, 251, 345
MseI TTAA 1 cut(s) 99
MvnI CGCG 1 cut(s) 96
MwoI GCNNNNNNNGC 3 cut(s) 61, 161, 265
NdeII GATC 5 cut(s) 18, 90, 123, 250, 373
NlaIII CATG 2 cut(s) 88, 226
NlaIV GGNNCC 2 cut(s) 65, 92
NmuCI GTSAC 1 cut(s) 110
NspV TTCGAA 1 cut(s) 305
PceI AGGCCT 2 cut(s) 35, 155
PcsI WCGNNNNNNNCGW 1 cut(s) 78
PfeI GAWTC 1 cut(s) 332
PkrI GCNGC 1 cut(s) 291
PleI GAGTC 1 cut(s) 19
PpsI GAGTC 1 cut(s) 19
PspN4I GGNNCC 2 cut(s) 65, 92
PspPI GGNCC 2 cut(s) 327, 337
PsuI RGATCY 3 cut(s) 18, 90, 123
RsaI GTAC 1 cut(s) 235
RsaNI GTAC 1 cut(s) 234
Rsr2I CGGWCCG 1 cut(s) 327
RsrII CGGWCCG 1 cut(s) 327
SalI GTCGAC 1 cut(s) 80
SaqAI TTAA 1 cut(s) 99
SatI GCNGC 1 cut(s) 290
Sau3AI GATC 5 cut(s) 18, 90, 123, 250, 373
Sau96I GGNCC 2 cut(s) 327, 337
SchI GAGTC 1 cut(s) 20
SetI ASST 3 cut(s) 112, 276, 280
SfaNI GCATC 1 cut(s) 153
SfuI TTCGAA 1 cut(s) 305
SinI GGWCC 2 cut(s) 327, 337
Sse9I AATT 1 cut(s) 307
SseBI AGGCCT 2 cut(s) 35, 155
SsiI CCGC 2 cut(s) 25, 94
SspMI CTAG 1 cut(s) 275
StuI AGGCCT 2 cut(s) 35, 155
StyI CCWWGG 3 cut(s) 150, 156, 254
TaaI ACNGT 2 cut(s) 68, 233
TaqI TCGA 3 cut(s) 81, 282, 305
TaqII GACCGA 1 cut(s) 344
TasI AATT 1 cut(s) 307
TfiI GAWTC 1 cut(s) 332
Tru1I TTAA 1 cut(s) 99
Tru9I TTAA 1 cut(s) 99
TseFI GTSAC 1 cut(s) 110
TseI GCWGC 1 cut(s) 289
Tsp45I GTSAC 1 cut(s) 110
TspGWI ACGGA 1 cut(s) 340
VpaK11BI GGWCC 2 cut(s) 327, 337
XmiI GTMKAC 1 cut(s) 81
XspI CTAG 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.