Rw2G031250

Asp/Glu/Hydantoin racemase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002475
Physical Location & Seq
Forward (+)
51337767 .. 51339430
1664 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G031250.1

Sequence Viewer

Length: 966 bp
ATGACAACTTCTGTTCACACTTTGAACTATCCTTTCCACAAATTGTGCTCTGTAAATACACATAAAACCCTCTACAAGACAAGGTGGAATCAGGGTTTAGCTCTAGCTCTATCCCCAGTTCTCTTGCAAACTAGGAAGTTTCCAGAGTATAAGAAGAATTCTGGTTTGAGTACCGCATGCAGCAATTCTTTTCTGGGTCAACCAAATACAATTGGTATAATTGGAGGGAAATCTGTCGATTCTACTGTTATTTTTCTGAAAAAACTATTTCACTGGAGTAAGAATGGAGAAAGTTTCCCTCCTTTTATTCTATGCTCTGATCCTGTGTTAAATAAAGAGCTGTTATTGACAGAGAGAAGCTCCTTACCTTGGCTTGACAGTAAAAATGAACGAACTAAACTTGATCCTACTTCAGTTGTGGAGAATCTGAGGAGTAAGAGGATTTATCTTGAAAATTCTGGTGCTCGTTGCATAGTAATGCCTTGTCATATTTCACATTCTTGGCATAATGAAATATCTGATGGATGTTCTGTTCCTTTCCTCCATATGGGTGAGTGTGTTGCCAAGGAGCTCAAGGAAGCAAAGTTGAAGCCACTTGAAGCCAGGAGTCCTTTGCGGATTGGAGTGCTTGCTACAAATGCAACTTTGACAGCAGGATTCTATCAGGAGAAACTGAAAAGGGAGGGATTTGAGGTTGTGTTGCCAGATAAAGCGACCATGGAACACACTGTCATCCCTGCTATTGAAGCTTTTAATAGAAAAGACATAGAGGGGTCACAAAATCTTTTGAGGATTGCACTCCAGGATCTTCTGGTGAGGGCTGTGAACTCTGTGATTCTTGCCTTTGATGATATGCATGATCTCCTTCCCCGAGATGATCCTCTTATTAAGAAATGCATTGACCCTATGGATGCATTGGCCTGGTCAACCATAAAATGGGCTACATCTGCTGAAAAAGGTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asp_Glu_race

Protein Analysis

321

Amino Acids

35.95

Weight (kDa)

8.81

Isoelectric Point (pI)

47.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp_Glu_race PF01177 132 - 309 7.8e-24 Asp/Glu/Hydantoin racemase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 936
AciI CCGC 2 cut(s) 174, 616
AclWI GGATC 4 cut(s) 314, 398, 813, 872
AcsI RAATTY 2 cut(s) 157, 454
AcuI CTGAAG 1 cut(s) 396
AfaI GTAC 2 cut(s) 172, 961
AfiI CCNNNNNNNGG 3 cut(s) 369, 547, 936
AgsI TTSAA 5 cut(s) 25, 452, 589, 599, 746
AjnI CCWGG 3 cut(s) 602, 801, 920
AloI GAACNNNNNNTCC 2 cut(s) 516, 548
AluBI AGCT 6 cut(s) 101, 107, 340, 360, 571, 749
AluI AGCT 6 cut(s) 101, 107, 340, 360, 571, 749
Alw21I GWGCWC 3 cut(s) 50, 466, 573
AlwI GGATC 4 cut(s) 314, 398, 813, 872
Ama87I CYCGRG 1 cut(s) 870
AoxI GGCC 1 cut(s) 918
ApeKI GCWGC 1 cut(s) 180
ApoI RAATTY 2 cut(s) 157, 454
AsuHPI GGTGA 2 cut(s) 563, 826
AvaI CYCGRG 1 cut(s) 870
BanII GRGCYC 1 cut(s) 573
Bbv12I GWGCWC 3 cut(s) 50, 466, 573
BbvI GCAGC 1 cut(s) 192
BccI CCATC 1 cut(s) 515
BciT130I CCWGG 3 cut(s) 604, 803, 922
BfaI CTAG 2 cut(s) 104, 132
BisI GCNGC 1 cut(s) 181
BlsI GCNGC 1 cut(s) 182
Bme1390I CCNGG 3 cut(s) 604, 803, 922
BmeT110I CYCGRG 1 cut(s) 870
BmrFI CCNGG 3 cut(s) 604, 803, 922
BmrI ACTGGG 1 cut(s) 110
BmsI GCATC 1 cut(s) 901
BmuI ACTGGG 1 cut(s) 110
BplI GAGNNNNNCTC 2 cut(s) 344, 376
BpmI CTGGAG 2 cut(s) 295, 785
BpuEI CTTGAG 1 cut(s) 557
BsaJI CCNNGG 3 cut(s) 368, 564, 717
Bsc4I CCNNNNNNNGG 3 cut(s) 369, 547, 936
Bse1I ACTGG 2 cut(s) 116, 278
BseBI CCWGG 3 cut(s) 604, 803, 922
BseDI CCNNGG 3 cut(s) 368, 564, 717
BseGI GGATG 3 cut(s) 530, 732, 916
BseLI CCNNNNNNNGG 3 cut(s) 369, 547, 936
BseMII CTCAG 1 cut(s) 419
BseNI ACTGG 2 cut(s) 116, 278
BseRI GAGGAG 1 cut(s) 445
BseXI GCAGC 1 cut(s) 192
BshFI GGCC 1 cut(s) 920
BsiHKAI GWGCWC 3 cut(s) 50, 466, 573
BsiHKCI CYCGRG 1 cut(s) 870
BslI CCNNNNNNNGG 3 cut(s) 369, 547, 936
BsnI GGCC 1 cut(s) 920
BsoBI CYCGRG 1 cut(s) 870
Bsp1286I GDGCHC 3 cut(s) 50, 466, 573
Bsp143I GATC 5 cut(s) 319, 403, 805, 859, 877
Bsp19I CCATGG 1 cut(s) 717
BspACI CCGC 2 cut(s) 174, 616
BspANI GGCC 1 cut(s) 920
BspCNI CTCAG 1 cut(s) 420
BspPI GGATC 4 cut(s) 314, 398, 813, 872
BsrI ACTGG 2 cut(s) 116, 278
BssECI CCNNGG 3 cut(s) 368, 564, 717
BssMI GATC 5 cut(s) 319, 403, 805, 859, 877
BssT1I CCWWGG 3 cut(s) 368, 564, 717
Bst2UI CCWGG 3 cut(s) 604, 803, 922
Bst4CI ACNGT 3 cut(s) 247, 380, 730
BstC8I GCNNGC 2 cut(s) 178, 630
BstDEI CTNAG 1 cut(s) 428
BstDSI CCRYGG 1 cut(s) 717
BstF5I GGATG 3 cut(s) 530, 732, 916
BstKTI GATC 5 cut(s) 322, 406, 808, 862, 880
BstMBI GATC 5 cut(s) 319, 403, 805, 859, 877
BstMWI GCNNNNNNNGC 3 cut(s) 638, 746, 947
BstNI CCWGG 3 cut(s) 604, 803, 922
BstNSI RCATGY 1 cut(s) 180
BstSCI CCNGG 3 cut(s) 602, 801, 920
BstV1I GCAGC 1 cut(s) 192
BstX2I RGATCY 1 cut(s) 805
BstYI RGATCY 1 cut(s) 805
BsuRI GGCC 1 cut(s) 920
BtgI CCRYGG 1 cut(s) 717
BtsCI GGATG 3 cut(s) 530, 732, 916
BtsIMutI CAGTG 2 cut(s) 271, 726
Cac8I GCNNGC 2 cut(s) 178, 630
Csp6I GTAC 2 cut(s) 171, 960
CviAII CATG 4 cut(s) 177, 718, 857, 963
CviQI GTAC 2 cut(s) 171, 960
DdeI CTNAG 1 cut(s) 428
DpnI GATC 5 cut(s) 321, 405, 807, 861, 879
DpnII GATC 5 cut(s) 319, 403, 805, 859, 877
Ecl136II GAGCTC 1 cut(s) 571
Eco130I CCWWGG 3 cut(s) 368, 564, 717
Eco24I GRGCYC 1 cut(s) 573
Eco53kI GAGCTC 1 cut(s) 571
Eco57I CTGAAG 1 cut(s) 396
Eco88I CYCGRG 1 cut(s) 870
EcoICRI GAGCTC 1 cut(s) 571
EcoRI GAATTC 1 cut(s) 157
EcoRII CCWGG 3 cut(s) 602, 801, 920
EcoT14I CCWWGG 3 cut(s) 368, 564, 717
EcoT22I ATGCAT 3 cut(s) 858, 899, 916
EcoT38I GRGCYC 1 cut(s) 573
ErhI CCWWGG 3 cut(s) 368, 564, 717
FaeI CATG 4 cut(s) 180, 721, 860, 966
FatI CATG 4 cut(s) 176, 717, 856, 962
FauNDI CATATG 1 cut(s) 546
Fnu4HI GCNGC 1 cut(s) 181
FokI GGATG 3 cut(s) 537, 719, 923
FriOI GRGCYC 1 cut(s) 573
Fsp4HI GCNGC 1 cut(s) 181
FspBI CTAG 2 cut(s) 104, 132
GluI GCNGC 1 cut(s) 181
GsuI CTGGAG 2 cut(s) 295, 785
HaeIII GGCC 1 cut(s) 920
Hin1II CATG 4 cut(s) 180, 721, 860, 966
HincII GTYRAC 2 cut(s) 200, 927
HindII GTYRAC 2 cut(s) 200, 927
HindIII AAGCTT 1 cut(s) 747
HinfI GANTC 6 cut(s) 88, 239, 424, 607, 657, 835
HphI GGTGA 2 cut(s) 563, 826
Hpy166II GTNNAC 4 cut(s) 16, 200, 826, 927
Hpy188I TCNGA 4 cut(s) 258, 319, 429, 520
Hpy188III TCNNGA 3 cut(s) 143, 449, 665
Hpy8I GTNNAC 4 cut(s) 16, 200, 826, 927
HpyAV CCTTC 1 cut(s) 875
HpyCH4III ACNGT 3 cut(s) 247, 380, 730
HpyCH4V TGCA 8 cut(s) 127, 180, 471, 641, 797, 856, 897, 914
HpyF10VI GCNNNNNNNGC 3 cut(s) 638, 746, 947
HpyF3I CTNAG 1 cut(s) 428
Hsp92II CATG 4 cut(s) 180, 721, 860, 966
Kzo9I GATC 5 cut(s) 319, 403, 805, 859, 877
LmnI GCTCC 2 cut(s) 365, 568
Lsp1109I GCAGC 1 cut(s) 192
LweI GCATC 1 cut(s) 901
MaeI CTAG 2 cut(s) 104, 132
MaeIII GTNAC 1 cut(s) 774
MalI GATC 5 cut(s) 321, 405, 807, 861, 879
MboI GATC 5 cut(s) 319, 403, 805, 859, 877
MboII GAAGA 2 cut(s) 166, 800
MfeI CAATTG 1 cut(s) 210
MflI RGATCY 1 cut(s) 805
MhlI GDGCHC 3 cut(s) 50, 466, 573
MluCI AATT 6 cut(s) 41, 157, 184, 210, 219, 454
MlyI GAGTC 1 cut(s) 616
Mph1103I ATGCAT 3 cut(s) 858, 899, 916
MseI TTAA 3 cut(s) 329, 753, 888
MslI CAYNNNNRTG 2 cut(s) 476, 549
MspR9I CCNGG 3 cut(s) 604, 803, 922
MunI CAATTG 1 cut(s) 210
MvaI CCWGG 3 cut(s) 604, 803, 922
MwoI GCNNNNNNNGC 3 cut(s) 638, 746, 947
NcoI CCATGG 1 cut(s) 717
NdeI CATATG 1 cut(s) 546
NdeII GATC 5 cut(s) 319, 403, 805, 859, 877
NlaIII CATG 4 cut(s) 180, 721, 860, 966
NmuCI GTSAC 1 cut(s) 774
NsiI ATGCAT 3 cut(s) 858, 899, 916
NspI RCATGY 1 cut(s) 180
PaeI GCATGC 1 cut(s) 180
PfeI GAWTC 5 cut(s) 88, 239, 424, 657, 835
PflMI CCANNNNNTGG 1 cut(s) 936
PfoI TCCNGGA 1 cut(s) 801
PkrI GCNGC 1 cut(s) 182
PleI GAGTC 1 cut(s) 615
PpsI GAGTC 1 cut(s) 615
Psp124BI GAGCTC 1 cut(s) 573
Psp6I CCWGG 3 cut(s) 602, 801, 920
PspGI CCWGG 3 cut(s) 602, 801, 920
PsuI RGATCY 1 cut(s) 805
RsaI GTAC 2 cut(s) 172, 961
RsaNI GTAC 2 cut(s) 171, 960
RseI CAYNNNNRTG 2 cut(s) 476, 549
SacI GAGCTC 1 cut(s) 573
SaqAI TTAA 3 cut(s) 329, 753, 888
SatI GCNGC 1 cut(s) 181
Sau3AI GATC 5 cut(s) 319, 403, 805, 859, 877
SchI GAGTC 1 cut(s) 616
ScrFI CCNGG 3 cut(s) 604, 803, 922
SduI GDGCHC 3 cut(s) 50, 466, 573
SfaNI GCATC 1 cut(s) 901
SmiMI CAYNNNNRTG 2 cut(s) 476, 549
SmlI CTYRAG 1 cut(s) 572
SmoI CTYRAG 1 cut(s) 572
SphI GCATGC 1 cut(s) 180
Sse9I AATT 6 cut(s) 41, 157, 184, 210, 219, 454
SsiI CCGC 2 cut(s) 174, 616
SspMI CTAG 2 cut(s) 104, 132
SstI GAGCTC 1 cut(s) 573
StyD4I CCNGG 3 cut(s) 602, 801, 920
StyI CCWWGG 3 cut(s) 368, 564, 717
TaaI ACNGT 3 cut(s) 247, 380, 730
TaqI TCGA 1 cut(s) 237
TasI AATT 6 cut(s) 41, 157, 184, 210, 219, 454
TfiI GAWTC 5 cut(s) 88, 239, 424, 657, 835
Tru1I TTAA 3 cut(s) 329, 753, 888
Tru9I TTAA 3 cut(s) 329, 753, 888
TscAI CASTG 2 cut(s) 278, 733
TseFI GTSAC 1 cut(s) 774
TseI GCWGC 1 cut(s) 180
Tsp45I GTSAC 1 cut(s) 774
TspDTI ATGAA 2 cut(s) 402, 525
TspRI CASTG 2 cut(s) 278, 733
Van91I CCANNNNNTGG 1 cut(s) 936
XapI RAATTY 2 cut(s) 157, 454
XceI RCATGY 1 cut(s) 180
XspI CTAG 2 cut(s) 104, 132
Zsp2I ATGCAT 3 cut(s) 858, 899, 916
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.