Alpha/beta hydrolase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 156 |
| AccB1I | GGYRCC | 1 cut(s) | 878 |
| AccII | CGCG | 1 cut(s) | 44 |
| AccIII | TCCGGA | 1 cut(s) | 502 |
| AciI | CCGC | 7 cut(s) | 44, 156, 223, 468, 491, 537, 920 |
| AcoI | YGGCCR | 1 cut(s) | 45 |
| AcsI | RAATTY | 1 cut(s) | 583 |
| AcyI | GRCGYC | 1 cut(s) | 879 |
| AdeI | CACNNNGTG | 1 cut(s) | 760 |
| AfaI | GTAC | 2 cut(s) | 329, 452 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 445, 799 |
| AflIII | ACRYGT | 1 cut(s) | 324 |
| AgeI | ACCGGT | 1 cut(s) | 438 |
| AgsI | TTSAA | 4 cut(s) | 88, 322, 673, 698 |
| AluBI | AGCT | 3 cut(s) | 462, 703, 745 |
| AluI | AGCT | 3 cut(s) | 462, 703, 745 |
| Alw26I | GTCTC | 1 cut(s) | 500 |
| Aor13HI | TCCGGA | 1 cut(s) | 502 |
| AoxI | GGCC | 3 cut(s) | 45, 781, 852 |
| ApoI | RAATTY | 1 cut(s) | 583 |
| AseI | ATTAAT | 1 cut(s) | 956 |
| AsiGI | ACCGGT | 1 cut(s) | 438 |
| AspLEI | GCGC | 1 cut(s) | 881 |
| AspS9I | GGNCC | 2 cut(s) | 210, 436 |
| AsuHPI | GGTGA | 2 cut(s) | 833, 927 |
| AvaII | GGWCC | 2 cut(s) | 210, 436 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 117, 117, 150, 150 |
| BanI | GGYRCC | 1 cut(s) | 878 |
| BbsI | GAAGAC | 1 cut(s) | 829 |
| BceAI | ACGGC | 2 cut(s) | 274, 481 |
| BciVI | GTATCC | 1 cut(s) | 145 |
| BclI | TGATCA | 3 cut(s) | 105, 553, 823 |
| BcoDI | GTCTC | 1 cut(s) | 500 |
| BfmI | CTRYAG | 1 cut(s) | 145 |
| BfoI | RGCGCY | 1 cut(s) | 882 |
| BfuI | GTATCC | 1 cut(s) | 145 |
| BisI | GCNGC | 3 cut(s) | 45, 469, 921 |
| BlsI | GCNGC | 3 cut(s) | 46, 470, 922 |
| Bme18I | GGWCC | 2 cut(s) | 210, 436 |
| BmgT120I | GGNCC | 2 cut(s) | 210, 436 |
| BmiI | GGNNCC | 2 cut(s) | 681, 880 |
| BmrI | ACTGGG | 1 cut(s) | 106 |
| BmsI | GCATC | 3 cut(s) | 580, 700, 766 |
| BmuI | ACTGGG | 1 cut(s) | 106 |
| BpiI | GAAGAC | 1 cut(s) | 829 |
| Bsa29I | ATCGAT | 1 cut(s) | 650 |
| BsaAI | YACGTR | 1 cut(s) | 331 |
| BsaHI | GRCGYC | 1 cut(s) | 879 |
| BsaWI | WCCGGW | 4 cut(s) | 378, 438, 502, 937 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 557, 587 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 445, 799 |
| Bse118I | RCCGGY | 2 cut(s) | 225, 438 |
| Bse1I | ACTGG | 1 cut(s) | 101 |
| BseAI | TCCGGA | 1 cut(s) | 502 |
| BseCI | ATCGAT | 1 cut(s) | 650 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 445, 799 |
| BseMII | CTCAG | 1 cut(s) | 313 |
| BseNI | ACTGG | 1 cut(s) | 101 |
| BseRI | GAGGAG | 1 cut(s) | 581 |
| Bsh1236I | CGCG | 1 cut(s) | 44 |
| Bsh1285I | CGRYCG | 1 cut(s) | 650 |
| BshFI | GGCC | 3 cut(s) | 47, 783, 854 |
| BshNI | GGYRCC | 1 cut(s) | 878 |
| BshTI | ACCGGT | 1 cut(s) | 438 |
| BshVI | ATCGAT | 1 cut(s) | 650 |
| BsiEI | CGRYCG | 1 cut(s) | 650 |
| BsiSI | CCGG | 6 cut(s) | 226, 335, 379, 439, 503, 938 |
| BslFI | GGGAC | 1 cut(s) | 288 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 445, 799 |
| BsmAI | GTCTC | 1 cut(s) | 500 |
| BsmBI | CGTCTC | 1 cut(s) | 500 |
| BsmFI | GGGAC | 1 cut(s) | 288 |
| BsnI | GGCC | 3 cut(s) | 47, 783, 854 |
| Bsp13I | TCCGGA | 1 cut(s) | 502 |
| Bsp143I | GATC | 6 cut(s) | 105, 525, 553, 625, 647, 823 |
| BspACI | CCGC | 7 cut(s) | 44, 156, 223, 468, 491, 537, 920 |
| BspANI | GGCC | 3 cut(s) | 47, 783, 854 |
| BspCNI | CTCAG | 1 cut(s) | 312 |
| BspDI | ATCGAT | 1 cut(s) | 650 |
| BspEI | TCCGGA | 1 cut(s) | 502 |
| BspFNI | CGCG | 1 cut(s) | 44 |
| BspLI | GGNNCC | 2 cut(s) | 681, 880 |
| BspQI | GCTCTTC | 1 cut(s) | 350 |
| BspT107I | GGYRCC | 1 cut(s) | 878 |
| BsrFI | RCCGGY | 2 cut(s) | 225, 438 |
| BsrI | ACTGG | 1 cut(s) | 101 |
| BssAI | RCCGGY | 2 cut(s) | 225, 438 |
| BssMI | GATC | 6 cut(s) | 105, 525, 553, 625, 647, 823 |
| BssNI | GRCGYC | 1 cut(s) | 879 |
| Bst4CI | ACNGT | 8 cut(s) | 25, 153, 175, 199, 266, 455, 511, 633 |
| Bst6I | CTCTTC | 2 cut(s) | 89, 350 |
| BstACI | GRCGYC | 1 cut(s) | 879 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 785 |
| BstBAI | YACGTR | 1 cut(s) | 331 |
| BstC8I | GCNNGC | 2 cut(s) | 781, 918 |
| BstDEI | CTNAG | 2 cut(s) | 299, 794 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 797 |
| BstFNI | CGCG | 1 cut(s) | 44 |
| BstH2I | RGCGCY | 1 cut(s) | 882 |
| BstHHI | GCGC | 1 cut(s) | 881 |
| BstKTI | GATC | 6 cut(s) | 108, 528, 556, 628, 650, 826 |
| BstMAI | GTCTC | 1 cut(s) | 500 |
| BstMBI | GATC | 6 cut(s) | 105, 525, 553, 625, 647, 823 |
| BstMCI | CGRYCG | 1 cut(s) | 650 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 267, 340, 468, 700, 770, 785 |
| BstNSI | RCATGY | 1 cut(s) | 920 |
| BstSFI | CTRYAG | 1 cut(s) | 145 |
| BstUI | CGCG | 1 cut(s) | 44 |
| BstV2I | GAAGAC | 1 cut(s) | 829 |
| Bsu15I | ATCGAT | 1 cut(s) | 650 |
| BsuI | GTATCC | 1 cut(s) | 145 |
| BsuRI | GGCC | 3 cut(s) | 47, 783, 854 |
| BsuTUI | ATCGAT | 1 cut(s) | 650 |
| Cac8I | GCNNGC | 2 cut(s) | 781, 918 |
| CfoI | GCGC | 1 cut(s) | 881 |
| Cfr10I | RCCGGY | 2 cut(s) | 225, 438 |
| Cfr13I | GGNCC | 2 cut(s) | 210, 436 |
| ClaI | ATCGAT | 1 cut(s) | 650 |
| Csp6I | GTAC | 2 cut(s) | 328, 451 |
| CspAI | ACCGGT | 1 cut(s) | 438 |
| CviAII | CATG | 4 cut(s) | 487, 623, 850, 917 |
| CviQI | GTAC | 2 cut(s) | 328, 451 |
| DdeI | CTNAG | 2 cut(s) | 299, 794 |
| DinI | GGCGCC | 1 cut(s) | 880 |
| DpnI | GATC | 6 cut(s) | 107, 527, 555, 627, 649, 825 |
| DpnII | GATC | 6 cut(s) | 105, 525, 553, 625, 647, 823 |
| DraIII | CACNNNGTG | 1 cut(s) | 760 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 156 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 156 |
| EaeI | YGGCCR | 1 cut(s) | 45 |
| Eam1104I | CTCTTC | 2 cut(s) | 89, 350 |
| EarI | CTCTTC | 2 cut(s) | 89, 350 |
| EciI | GGCGGA | 1 cut(s) | 506 |
| Eco147I | AGGCCT | 1 cut(s) | 783 |
| Eco47I | GGWCC | 2 cut(s) | 210, 436 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 797 |
| EcoRI | GAATTC | 1 cut(s) | 583 |
| EgeI | GGCGCC | 1 cut(s) | 880 |
| EheI | GGCGCC | 1 cut(s) | 880 |
| Esp3I | CGTCTC | 1 cut(s) | 500 |
| FaeI | CATG | 4 cut(s) | 490, 626, 853, 920 |
| FaiI | YATR | 6 cut(s) | 50, 410, 488, 624, 851, 918 |
| FaqI | GGGAC | 1 cut(s) | 288 |
| FatI | CATG | 4 cut(s) | 486, 622, 849, 916 |
| FauI | CCCGC | 1 cut(s) | 530 |
| FbaI | TGATCA | 3 cut(s) | 105, 553, 823 |
| Fnu4HI | GCNGC | 3 cut(s) | 45, 469, 921 |
| Fsp4HI | GCNGC | 3 cut(s) | 45, 469, 921 |
| GlaI | GCGC | 1 cut(s) | 880 |
| GluI | GCNGC | 3 cut(s) | 45, 469, 921 |
| HaeII | RGCGCY | 1 cut(s) | 882 |
| HaeIII | GGCC | 3 cut(s) | 47, 783, 854 |
| HapII | CCGG | 6 cut(s) | 226, 335, 379, 439, 503, 938 |
| HhaI | GCGC | 1 cut(s) | 881 |
| Hin1I | GRCGYC | 1 cut(s) | 879 |
| Hin1II | CATG | 4 cut(s) | 490, 626, 853, 920 |
| Hin6I | GCGC | 1 cut(s) | 879 |
| HinP1I | GCGC | 1 cut(s) | 879 |
| HincII | GTYRAC | 2 cut(s) | 178, 296 |
| HindII | GTYRAC | 2 cut(s) | 178, 296 |
| HinfI | GANTC | 7 cut(s) | 117, 253, 386, 601, 643, 932, 962 |
| HpaII | CCGG | 6 cut(s) | 226, 335, 379, 439, 503, 938 |
| HphI | GGTGA | 2 cut(s) | 833, 927 |
| Hpy166II | GTNNAC | 6 cut(s) | 178, 202, 296, 328, 436, 451 |
| Hpy188I | TCNGA | 4 cut(s) | 116, 252, 376, 589 |
| Hpy188III | TCNNGA | 6 cut(s) | 208, 503, 529, 683, 800, 827 |
| Hpy8I | GTNNAC | 6 cut(s) | 178, 202, 296, 328, 436, 451 |
| HpyAV | CCTTC | 2 cut(s) | 511, 556 |
| HpyCH4III | ACNGT | 8 cut(s) | 25, 153, 175, 199, 266, 455, 511, 633 |
| HpyCH4IV | ACGT | 2 cut(s) | 324, 330 |
| HpyCH4V | TGCA | 5 cut(s) | 171, 486, 751, 779, 865 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 267, 340, 468, 700, 770, 785 |
| HpyF3I | CTNAG | 2 cut(s) | 299, 794 |
| HpySE526I | ACGT | 2 cut(s) | 324, 330 |
| Hsp92I | GRCGYC | 1 cut(s) | 879 |
| Hsp92II | CATG | 4 cut(s) | 490, 626, 853, 920 |
| HspAI | GCGC | 1 cut(s) | 879 |
| KasI | GGCGCC | 1 cut(s) | 878 |
| Kpn2I | TCCGGA | 1 cut(s) | 502 |
| Ksp22I | TGATCA | 3 cut(s) | 105, 553, 823 |
| Kzo9I | GATC | 6 cut(s) | 105, 525, 553, 625, 647, 823 |
| LguI | GCTCTTC | 1 cut(s) | 350 |
| LmnI | GCTCC | 1 cut(s) | 381 |
| LweI | GCATC | 3 cut(s) | 580, 700, 766 |
| MaeII | ACGT | 2 cut(s) | 324, 330 |
| MalI | GATC | 6 cut(s) | 107, 527, 555, 627, 649, 825 |
| MboI | GATC | 6 cut(s) | 105, 525, 553, 625, 647, 823 |
| MboII | GAAGA | 3 cut(s) | 76, 337, 829 |
| MluCI | AATT | 4 cut(s) | 583, 737, 813, 890 |
| Mly113I | GGCGCC | 1 cut(s) | 879 |
| MlyI | GAGTC | 3 cut(s) | 395, 652, 971 |
| MnlI | CCTC | 8 cut(s) | 44, 50, 80, 149, 254, 406, 559, 570 |
| MroI | TCCGGA | 1 cut(s) | 502 |
| MseI | TTAA | 3 cut(s) | 183, 956, 982 |
| MspI | CCGG | 6 cut(s) | 226, 335, 379, 439, 503, 938 |
| MvnI | CGCG | 1 cut(s) | 44 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 267, 340, 468, 700, 770, 785 |
| NarI | GGCGCC | 1 cut(s) | 879 |
| NdeII | GATC | 6 cut(s) | 105, 525, 553, 625, 647, 823 |
| NlaIII | CATG | 4 cut(s) | 490, 626, 853, 920 |
| NlaIV | GGNNCC | 2 cut(s) | 681, 880 |
| NspI | RCATGY | 1 cut(s) | 920 |
| PaeI | GCATGC | 1 cut(s) | 920 |
| PceI | AGGCCT | 1 cut(s) | 783 |
| PciSI | GCTCTTC | 1 cut(s) | 350 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 471 |
| PfeI | GAWTC | 4 cut(s) | 117, 253, 601, 932 |
| PinAI | ACCGGT | 1 cut(s) | 438 |
| PkrI | GCNGC | 3 cut(s) | 46, 470, 922 |
| Ple19I | CGATCG | 1 cut(s) | 650 |
| PleI | GAGTC | 3 cut(s) | 394, 651, 970 |
| PluTI | GGCGCC | 1 cut(s) | 882 |
| PpsI | GAGTC | 3 cut(s) | 394, 651, 970 |
| Ppu21I | YACGTR | 1 cut(s) | 331 |
| PshBI | ATTAAT | 1 cut(s) | 956 |
| PspN4I | GGNNCC | 2 cut(s) | 681, 880 |
| PspPI | GGNCC | 2 cut(s) | 210, 436 |
| PvuI | CGATCG | 1 cut(s) | 650 |
| RsaI | GTAC | 2 cut(s) | 329, 452 |
| RsaNI | GTAC | 2 cut(s) | 328, 451 |
| SapI | GCTCTTC | 1 cut(s) | 350 |
| SaqAI | TTAA | 3 cut(s) | 183, 956, 982 |
| SatI | GCNGC | 3 cut(s) | 45, 469, 921 |
| Sau3AI | GATC | 6 cut(s) | 105, 525, 553, 625, 647, 823 |
| Sau96I | GGNCC | 2 cut(s) | 210, 436 |
| SchI | GAGTC | 3 cut(s) | 395, 652, 971 |
| SetI | ASST | 9 cut(s) | 42, 72, 327, 333, 374, 464, 522, 705, 747 |
| SfaNI | GCATC | 3 cut(s) | 580, 700, 766 |
| SfcI | CTRYAG | 1 cut(s) | 145 |
| SfoI | GGCGCC | 1 cut(s) | 880 |
| SinI | GGWCC | 2 cut(s) | 210, 436 |
| SphI | GCATGC | 1 cut(s) | 920 |
| Sse9I | AATT | 4 cut(s) | 583, 737, 813, 890 |
| SseBI | AGGCCT | 1 cut(s) | 783 |
| SsiI | CCGC | 7 cut(s) | 44, 156, 223, 468, 491, 537, 920 |
| SspDI | GGCGCC | 1 cut(s) | 878 |
| StuI | AGGCCT | 1 cut(s) | 783 |
| TaaI | ACNGT | 8 cut(s) | 25, 153, 175, 199, 266, 455, 511, 633 |
| TaiI | ACGT | 2 cut(s) | 327, 333 |
| TaqI | TCGA | 3 cut(s) | 231, 646, 650 |
| TasI | AATT | 4 cut(s) | 583, 737, 813, 890 |
| TauI | GCSGC | 3 cut(s) | 47, 471, 923 |
| TfiI | GAWTC | 4 cut(s) | 117, 253, 601, 932 |
| Tru1I | TTAA | 3 cut(s) | 183, 956, 982 |
| Tru9I | TTAA | 3 cut(s) | 183, 956, 982 |
| TspGWI | ACGGA | 1 cut(s) | 281 |
| VpaK11BI | GGWCC | 2 cut(s) | 210, 436 |
| VspI | ATTAAT | 1 cut(s) | 956 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 797 |
| XapI | RAATTY | 1 cut(s) | 583 |
| XceI | RCATGY | 1 cut(s) | 920 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 406 |